Induction of differentiation by radiation and hyperthermia in neuroblastoma-glioma hybrid cells.

Abstract:

:The effects of either radiation or hyperthermia on the differentiation potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108-15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action potential and a diphasic (inward following outward) current, respectively. An increase in the specific activity of acetylcholinesterase was also observed. In addition, both treatments induced an elevation of the concentration of intracellular calcium in some cells. The increase in intracellular calcium concentration caused by applying the calcium ionophore, A23187, induced differentiation. It is suggested that both the radiation- and the hyperthermia-induced increases of electrical excitability and acetylcholinesterase activity may have originated from an increase in intracellular Ca2+ concentration.

journal_name

Cell Biochem Funct

authors

Sugihara M,Fujita Y,Enomoto K,Maeno T,Ishida T

doi

10.1002/cbf.290120209

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

137-42

issue

2

eissn

0263-6484

issn

1099-0844

journal_volume

12

pub_type

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