Abstract:
:Vascular endothelial growth factor (VEGF) is an endothelium-specific mitogen and a promising inducer of angiogenesis and lymphangiogenesis. The VEGF receptors on endothelial cell membrane include the tyrosine kinases VEGFR-1 (Flt-1), VEGFR-2 (Flk-1/KDR) and VEGFR-3 (Flt-4). KDR is a major mediator of mitogenic, angiogenic and permeability-enhancing effects of VEGF. KDR is upregulated in response to hypoxia, a major inducer of VEGF gene transcription. A HEK293 cell line overexpressing KDR was established under cell hypoxic stress to explore the function of KDR. A hypoxia-inducing agent, cobalt chloride (CoCl(2)) was applied to detect whether KDR was able to prevent against chemical hypoxic toxicity. The results indicate that KDR attenuated CoCl(2)-induced cell injury in HEK293 cells. Furthermore, the underlying mechanisms may be explained by the increased expression of Bcl-2, AKT1 and phosphorylated AKT, key members of cell survival pathway, and decreased expression of pro-apoptosis protein Bax.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Ge X,Zhao L,He L,Chen W,Li Xdoi
10.1002/cbf.1829subject
Has Abstractpub_date
2012-03-01 00:00:00pages
151-7issue
2eissn
0263-6484issn
1099-0844journal_volume
30pub_type
杂志文章abstract::Hyperglycemia induces overproduction of superoxide and it is related to diabetic complications. In this study, we analyzed the antioxidant enzymatic defense and the lipid peroxidation of rat salivary glands in six different periods of diabetic condition. Ninety-six rats were divided into 12 groups: C7/14/21/28/45/60 (...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1683
更新日期:2010-08-01 00:00:00
abstract::Diabetes induces oxidative stress in aged human and rat, although daily supplementation of vitamins C and E (VCE) can be beneficial to aged diabetic rats by reducing free radical production. The aim of the present study was to evaluate whether dietary VCE supplementation relieves oxidative stress in streptozotocin (ST...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1749
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journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1215
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journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2918
更新日期:2013-07-01 00:00:00
abstract::Human adipose-derived stromal/stem cells (ASC) have immunomodulatory properties and the potential to differentiate into several cell lines, important for application in regenerative medicine. However, the contamination with dermal fibroblasts (FIB) can impair the beneficial effects of ASC in cell therapy. It is then e...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3610
更新日期:2021-01-03 00:00:00
abstract::In pancreatic islets prepared from fed rats and incubated at a low concentration (1.7 mM) of D-glucose, D-mannoheptulose (10.0 mM) virtually fails to affect the metabolism of the hexose. Likewise, in islets from starved rats, the relative extent of the inhibitory action of D-mannoheptulose upon D-glucose metabolism is...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
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abstract::The protective effects of carnosine as a natural dipeptide were investigated in mouse bone marrow cells against genotoxicity induced by cyclophosphamide. Mice were injected with solutions of carnosine at three different doses (10, 50 and 100 mg kg(-1) bw) for five consecutive days. On the fifth day of treatment, mice ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1294
更新日期:2006-11-01 00:00:00
abstract::Glucocorticoids are important for skeletal muscle energy metabolism, regulating glucose utilization, insulin sensitivity, and muscle mass. Nicotinamide adenine dinucleotide phosphate-dependent 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)-mediated glucocorticoid activation in the sarcoplasmic reticulum (SR) is in...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3272
更新日期:2017-07-01 00:00:00
abstract::We report a functional characterisation of the novel protein MGC13096. MGC13096 over-expression restrained proliferation of HEK293T cells. DNA/flow cytometry analysis showed that the over-expression of MGC13096 severely delays cell cycle progression at S phase. Transcription factors such as activator protein-1 (AP-1),...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1410
更新日期:2008-03-01 00:00:00
abstract::MicroRNA (miRNAs) are short non-coding RNA molecules that downregulate gene expression at post-transcriptional level. miRNAs are post-transcriptional regulators of gene expression important for neuron development and function. This report demonstrated that a putative and chemically synthesized miRNA rno-mir-541 played...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1747
更新日期:2011-06-01 00:00:00
abstract::During remodelling of pulmonary artery, marked proliferation of pulmonary artery smooth muscle cells (PASMCs) occurs, which contributes to pulmonary hypertension. Thromboxane A2 (TxA2) has been shown to produce pulmonary hypertension. The present study investigates the inhibitory effect of epigallocatechin-3-gallate (...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3136
更新日期:2015-10-01 00:00:00
abstract::Organochalcogens are extensively produced and employed by industry and agriculture, and the risk of occupational and environmental toxicity to them has been poorly understood. Here, we investigated the acute effect of a new organochalcogen 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on biochemical and hematologic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2806
更新日期:2012-06-01 00:00:00
abstract::Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1612
更新日期:2009-12-01 00:00:00
abstract::The gene expression of heme oxygenase-1 (HO-1) was studied in mammalian cell lines exposed to hyperoxia. Northern blot analysis demonstrated that hyperoxic exposure increased the HO-1 mRNA levels in various types of cells, including human hepatoma (HepG2) cells. This increase was time- and dose-dependent, and reversib...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199809)16:3<183::AID-CBF78
更新日期:1998-09-01 00:00:00
abstract::The object was to determine the influence of dietary vitamin E, selenium and their combination on the fatty acid content of erythrocytes, bone marrow and spleen lipids of Akkaraman lambs. After supplementation for 15 days, the amount of all fatty acids was slightly higher (p < 0.05) in the vitamin E as compared to the...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199703)15:1<1::AID-CBF694>
更新日期:1997-03-01 00:00:00
abstract::The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF7
更新日期:1997-06-01 00:00:00
abstract::It is generally accepted that hypertension and other vascular pathologies increase in diabetes mellitus (DM) patients as a result of the renin-angiotensin-aldosterone (RAA) system. In this study, changes in the renin-angiotensin-aldosterone (RAA) system level was determined in Streptozotocin (STZ)-injected rats. A tot...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199909)17:3<193::AID-CBF82
更新日期:1999-09-01 00:00:00
abstract::Familial Mediterranean Fever (FMF) is an autosomal recessive hereditary disease leading mostly to renal failure and nephrotic syndrome. The ultrastructure of kidney has not been fully investigated in FMF associated renal disease. The aim of this study is to provide further evidence on the ultrastructure of kidney in p...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1286
更新日期:2007-07-01 00:00:00
abstract::Astroglial cells dispersed from newborn rat hemispheres were established in medium supplemented with 20 per cent fetal calf serum (FBS) and then grown to a confluent monolayer in the presence of 10 per cent FBS or charcoal-stripped FBS (CS). Type 1 astrocytes were subcultured and either maintained under the same condi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.645
更新日期:1996-03-01 00:00:00
abstract::Pig thyroid Golgi vesicles incubated in a suitable medium were able to concentrate iodide from the medium. This trapping required the integrity of the vesicles, was time- and temperature-dependent, and was inhibited by a competitive inhibitor of iodide active transport (perchlorate), suggesting a facilitated transport...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030106
更新日期:1985-01-01 00:00:00
abstract::The aim of this experimental study was to investigate the possible role of adenosine deaminase (AD) and xanthine oxidase (XO) in the pathogenesis of cisplatin-induced nephrotoxicity and the effect of erdosteine in decreasing the toxicity. The intraperitoneal injection of cisplatin (7 mg kg(-1) body weight) induced a s...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1069
更新日期:2004-05-01 00:00:00
abstract::The heart responds to an increased demand arising due to physiological stimuli or pathological insults by hypertrophy of myocytes. Reactive oxygen species (ROS) have recently been identified as the molecular intermediates in the translation of mechanical stimuli to cellular response. Different signal transduction path...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1449
更新日期:2008-04-01 00:00:00
abstract::In view of the antioxidant properties of melatonin, the effects of melatonin on the oxidative-antioxidative status of tissues affected by diabetes, e.g. liver, heart and kidneys, were investigated in streptozotocin (STZ)-induced diabetic rats in the present study. Concentrations of malondialdehyde (MDA) and reduced gl...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1006
更新日期:2003-06-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::Ras-GTPases regulate many central signalling pathways in the cell. Hypoxia induces nitrosative/oxidative stress and dysregulates Ras-dependent downstream processes. H-Ras possesses two cysteine residues (C181 and C184) in the C-termini, which are palmitoylated once or twice. Palmitoylation is sufficient for promoting ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3431
更新日期:2019-10-01 00:00:00
abstract::Incidence and mortality rates of cancer continue to increase greatly despite the improved diagnostic and therapeutic methods. Based on GLOBOCAN estimates, the numbers of new cancer cases reported in 2018 were ~18.1 million, while the numbers of cancer mortalities were ~9.6 million. It remains difficult to diagnose mos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3601
更新日期:2020-11-06 00:00:00
abstract::Mitochondrial, endoplasmic reticular and plasma membrane fractions were isolated by a new method from control male Fischer 344 rats and rats given CCl4 by gavage. After 1 h of CCl4 treatment, rats were in glucose and pancreatic hormone balance but plasma levels of T3 and T4 were decreased 29 and 22%, respectively. Aft...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.934
更新日期:2002-03-01 00:00:00
abstract::Psoriasis is a common chronic and recurrent inflammatory skin disease with unknown etiology that has been associated with abnormal plasma lipid metabolism and oxidative stress. There are controversial results in the previous studies investigating oxidant/antioxidant systems in psoriasis.The aim of this work was to eva...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1553
更新日期:2009-04-01 00:00:00
abstract::Regeneration remains a major challenge in skeletal muscle repair after injury. Recently, transforming growth factor-β (TGF-β)/Smad pathway was found to play an important role in inhibiting myogenesis, a crucial stage in skeletal muscle regeneration. In our previous study, microRNA-122-5p (miR-122) was proved to have t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3460
更新日期:2020-03-01 00:00:00