Abstract:
:Studies in the rat and rabbit indicate that facilitatory effects of neuropeptide Y (NPY) as well as norepinephrine (NE) on LH and LHRH release are dependent on the presence of the ovarian steroid estrogen. However, we have previously found the NE and an alpha-1-adrenergic agonist are both stimulatory to pulsatile LHRH release in ovariectomized rhesus monkeys. In the present experiment the effects of NPY on LHRH release were examined in conscious monkeys using a push-pull perfusion method. Twelve gonadectomized monkeys (8 females and 4 males) were used. Perfusate samples from the stalk-median eminence (S-ME) were obtained through a push-pull cannula at 10-min intervals for 12 h, and the amount of LHRH in samples were determined with RIA. NPY dissolved in a modified Krebs-Ringer phosphate buffer solution at concentrations of 10(-8), 10(-7), 10(-6), and 10(-5) M was directly infused into the S-ME through the push cannula for 10 min at 90-min intervals. Vehicle was infused as a control. Since sex differences in LHRH response to NPY were not present, data from males and females were combined for analysis. NPY infusion into the S-ME stimulated LHRH release in a dose-dependent manner (P less than 0.001). The peak LHRH responses (mean +/- SEM) to NPY at different concentrations were: 10(-8) M = 2.1 +/- 0.4 pg/ml; 10(-7) M = 2.6 +/- 0.5 pg/ml; 10(-6) M = 6.5 +/- 1.1 pg/ml; 10(-5) M = 15.1 +/- 2.9 pg/ml, whereas to vehicle 0.37 +/- 0.17 pg/ml. All NPY doses tested were significantly effective as compared to vehicle (P less than 0.01). The LHRH response to 10(-6) M was greater (P less than 0.01) than that of 10(-8) M or 10(-7) M, and the response to 10(-5) M was greater (P less than 0.01) than that of all lower doses. The results indicate that NPY infusion into the S-ME elicits the release of LHRH in vivo in a dose-dependent manner in the monkey. The data further suggest that LHRH neurons and/or neuroterminals in the monkey are responsive to NPY stimulation in the absence of gonadal steroids. It is concluded that in addition to NE, NPY is an important regulator of pulsatile LHRH release in the nonhuman primate.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Woller MJ,Terasawa Edoi
10.1210/endo-128-2-1144subject
Has Abstractpub_date
1991-02-01 00:00:00pages
1144-50issue
2eissn
0013-7227issn
1945-7170journal_volume
128pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Isolated renal cortical slices were used to study the conversion of 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and 24,25-dihydroxyvitamin D3 [24,25](OH2)D3] by the rat kidney. Production of 1,25-(OH)2D3 and 24,25-(OH)2D3 was linear with time (30-90 min) and tissue weight (40-250 mg). Production of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-218
更新日期:1981-07-01 00:00:00
abstract::The uptake of [125I]triiodothyroacetic acid ([125I]Triac) in anterior pituitary cells was investigated and compared with that of [125I]T3. Furthermore, the effects of Triac, T3, and T4 on TSH release were compared. Cells isolated from adult male Wistar rats were cultured for 3 days in medium with 10% fetal calf serum....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988460
更新日期:1994-12-01 00:00:00
abstract::In dairy goats, treatments associating a progestogen and the equine chorionic gonadotropin (eCG) are the easiest way to induce and synchronize estrus and ovulation and to permit artificial insemination (AI) and/or out of season breeding. From the first treatment, the injection of eCG induces, in some females, the prod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0595
更新日期:2004-01-01 00:00:00
abstract::To investigate further brain-pituitary-gonadal interrelationships we have generated mice in which the gene encoding the FSH receptor has been disrupted. Female FSH receptor knockout (FSHRKO) mice were infertile. The ovaries were significantly reduced in size, with follicular development arrested at the preantral stage...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7456
更新日期:2000-05-01 00:00:00
abstract::Metastatic pheochromocytoma continues to be an incurable disease, and treatment with conventional cytotoxic chemotherapy offers limited efficacy. In the present study, we evaluated a novel topoisomerase I inhibitor, LMP-400, as a potential treatment for this devastating disease. We found a high expression of topoisome...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1476
更新日期:2015-11-01 00:00:00
abstract::When colonic crypt cells isolated from intact rats are incubated with [3H]corticosterone specific nuclear binding is displaced by neither aldosterone nor the antiglucocorticoid RU38486, suggesting that [3H]corticosterone is binding to a site distinct from classical mineralocorticoid and glucocorticoid receptors. TLC r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754750
更新日期:1996-08-01 00:00:00
abstract::Mutations of the monocarboxylate transporter 8 gene (MCT8, SLC16A2) cause the Allan-Herndon-Dudley syndrome, an X-linked syndrome of severe intellectual deficit and neurological impairment. Mct8 transports thyroid hormones (T4 and T3), and the Allan-Herndon-Dudley syndrome is likely caused by lack of T3 transport to n...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1014
更新日期:2011-04-01 00:00:00
abstract::The low-density lipoprotein receptor (Ldlr) is a key molecule involved with lipid clearance. The Ldlr(-/-) mouse has been used extensively as a model for studying atherosclerosis. This study sought to characterize the energy balance phenotype of Ldlr(-/-) mice with respect to weight gain, body composition, energy expe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0496
更新日期:2010-11-01 00:00:00
abstract::Previously, a human CYP3A4-transgenic (Tg-CYP3A4) mouse line was reported to exhibit enhanced metabolism of midazolam by cytochrome P450 3A4 (CYP3A4) expressed in small intestine. Here we show that expression of CYP3A4 and murine cyp3a and cyp2b was both age and sex dependent. CYP3A4 was expressed in the livers of mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1248
更新日期:2005-07-01 00:00:00
abstract::Thyroid hormones are essential for normal development and metabolism. Thyroid hormone biosynthesis requires iodide uptake into the thyrocytes and efflux into the follicular lumen, where it is organified on selected tyrosyls of thyroglobulin. Uptake of iodide into the thyrocytes is mediated by an intrinsic membrane gly...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2008-1437
更新日期:2009-03-01 00:00:00
abstract::The PTH and PTH-related protein (PTHrP) system consists of two hormones, at least two G protein-coupled seven-transmembrane domain receptors, and at least two intracellular signal transduction pathways for each receptor. The PTH/PTHrP receptor is present in the conventional target tissues of PTH action, namely kidney ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.10.8828480
更新日期:1996-10-01 00:00:00
abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0473
更新日期:2006-09-01 00:00:00
abstract::Parathyroid tissue from patients with hyperparathyroidism (HPT) exhibited reduced immunohistochemical reactivity with monoclonal antiparathyroid antibodies, previously shown to stain intensely the surface of normal human parathyroid cells and to interfere with a receptor mechanism of these cells which is involved in t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2999
更新日期:1988-06-01 00:00:00
abstract::In agonadal juvenile male monkeys, continuous administration of human metastin 45-54 (hu metastin 45-54) leads to desensitization of its receptor, G protein-coupled receptor 54 (GPR54), and decreased LH. The present study extended this observation to the adult male monkey, a more preclinically relevant model in which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0207
更新日期:2007-07-01 00:00:00
abstract::We examined the effects of bombesin on rat pancreatic digestive enzyme gene expression using cloned complementary DNA probes for amylase, trypsinogen I, chymotrypsinogen B, and lysophospholipase. Rats were injected sc three times daily with 5 nmol/kg body wt bombesin. Pancreata were investigated after 6, 12, 24, 48, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1371450
更新日期:1992-03-01 00:00:00
abstract::The binding characteristics of 125I-labeled Leu15-gastrin and the molecular identification of the gastrin receptor was investigated in dispersed guinea pig gastric glands. The binding of [125I]gastrin to gastric glands was temperature dependent, saturable, and specific. At 37 C, the binding was rapid, became maximal w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-1881
更新日期:1989-04-01 00:00:00
abstract::Using a primary culture of neonatal rat heart, we investigated the properties of iodothyronine 5'-deiodinating activity in the heart. 125I- release from [125I] reverse T3 incubated with cell homogenates depended on the concentrations of protein and dithiothreitol, pH, incubation time, and temperature of the incubation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3105
更新日期:1991-06-01 00:00:00
abstract::High density lipoprotein (HDL) participates in reverse cholesterol transport and in the delivery of cholesterol to steroid-producing tissues. Scavenger receptor class B type I (SR-BI) was recently shown to bind HDL and mediate internalization of its cholesterol content. We have cloned the rat homolog of this receptor,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5666
更新日期:1998-01-01 00:00:00
abstract::The receptor for the orexigenic peptide, ghrelin, is one of the most constitutively active 7TM receptors known, as demonstrated under in vitro conditions. Change in expression of a constitutively active receptor is associated with change in signaling independent of the endogenous ligand. In the following study, we fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1638
更新日期:2009-11-01 00:00:00
abstract::In this study the hypothesis that the LHRH neurosecretory system of the prepubertal female monkey has the capacity to function in a manner comparable to that of monkeys in more mature stages of development was tested. Using push-pull perfusion in the stalk-median eminence, effects of electrical stimulation of the medi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3014
更新日期:1990-12-01 00:00:00
abstract::In contrast to other systems in which angiotensin-II (AII) inhibits adenylyl cyclase, in fetal skin fibroblasts the peptide stimulates cAMP accumulation. The mechanism of this novel effect was studied by analysis of the actions of AII and other regulators on the adenylyl cyclase system in cultured cells. In the presen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1330500
更新日期:1992-11-01 00:00:00
abstract::The monocarboxylate transporter Mct10 (Slc16a10; T-type amino acid transporter) facilitates the cellular transport of thyroid hormone (TH) and shows an overlapping expression with the well-established TH transporter Mct8. Because Mct8 deficiency is associated with distinct tissue-specific alterations in TH transport a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1800
更新日期:2014-01-01 00:00:00
abstract::Bone marrow-derived cells are major cellular components of human decidua, with macrophages comprising about 30% of the cells in term tissue. Because cytokines released by bone marrow-derived cells are known to affect hormone release in many tissues, we examined whether cytokines affect the release of PRL from human de...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275950
更新日期:1994-01-01 00:00:00
abstract::In several mammalian species, luteinizing hormone-releasing hormone (LHRH) neurons have been shown to migrate from nasal regions to the brain during early development. Using immunocytochemistry, we have identified LHRH containing neurons in developing chick embryos. In embryonic day 4 (E4) and E5 animals, a small grou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1676
更新日期:1991-03-01 00:00:00
abstract::Glucose release from the liver is mediated by hypothalamic norepinephrine (NE) neuronal activity, but glucose itself (or a metabolite of it) exerts negative feedback effects on central NE activity. The aim of the present study was to investigate a possible role for insulin in these central glucose homeostatic mechanis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1590
更新日期:1985-10-01 00:00:00
abstract::We are investigating the mechanisms by which aromatic hydrocarbons, such as 3-methylcholanthrene (MC), suppress hepatic cytochrome P450 2C11 (CYP2C11) gene expression. CYP2C11 is an enzyme expressed in the liver of male rats and is regulated by a pulsatile pattern of GH secretion. We have previously shown that MC atte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220212
更新日期:2002-09-01 00:00:00
abstract::The purpose of this investigation was to use an approach targeted specifically on endogenous relaxin to determine the influence of relaxin on birth in the rat. To that end, a monoclonal antibody specific for rat relaxin, designated MCA1, was used to passively neutralize endogenous relaxin in intact pregnant rats. MCA1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2479
更新日期:1988-11-01 00:00:00
abstract::Congenital hypothyroidism with thyroid dysgenesis (TD) is a frequent human condition characterized by elevated levels of TSH in response to reduced thyroid hormone levels. Congenital hypothyroidism is a genetically heterogeneous disease. In the majority of cases studied, no causative mutations have been identified and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0882
更新日期:2005-12-01 00:00:00
abstract::Impaired wound healing of skin is one of the most serious complications of diabetes. However, the pathogenesis of this process is not known, and it is unclear whether impaired insulin signaling could directly affect skin physiology. To elucidate the role of insulin in skin, we studied skin insulin receptor (IR) null m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.7988
更新日期:2001-03-01 00:00:00
abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013350
更新日期:1994-07-01 00:00:00