Abstract:
:The uptake of [125I]triiodothyroacetic acid ([125I]Triac) in anterior pituitary cells was investigated and compared with that of [125I]T3. Furthermore, the effects of Triac, T3, and T4 on TSH release were compared. Cells isolated from adult male Wistar rats were cultured for 3 days in medium with 10% fetal calf serum. Uptake was measured at 37 C with [125I]Triac (100,000 cpm; 120 pM) or [125I]T3 (50,000 cpm; 50 pM) in medium with 0.5% BSA. In this medium, the ratio of the free fractions of Triac, T3, and T4 was 1:8:1. Exposure of cells to 100 nM TRH for 2 h stimulated TSH release by 80-110% (P < 0.001). Comparing total hormone levels (1 nM to 1 microM), Triac and T3 were equally effective in reducing this response, and both were 10-fold more effective than T4. The time course (15 min to 4 h) of [125I]Triac uptake was similar to that of [125I]T3, showing equilibrium after 1 h. Unlabeled Triac (1 microM) reduced the uptake of [125I]Triac and [125I]T3 at all time intervals. Expressed per pM free hormone, the cellular and nuclear uptake of [125I]Triac were twice those of [125I]T3. The 15-min uptake of [125I]Triac was reduced by incubation with 10 nM unlabeled Triac (35%; P < 0.001). Maximum inhibition (56%; P < 0.001) was found with 10 microM Triac. A similar effect was seen with 10 microM T3, T4, or 3,3',5,5'-tetraiodothyroacetic acid. Preincubation (30 min) and incubation (15 min) with 10 microM oligomycin reduced the cellular ATP content by 51% (P < 0.001), [125I]T3 uptake by 77% (P < 0.001), and [125I]Triac uptake by only 25% (P < 0.001). The temperature dependence of [125I]Triac and [125I]T3 uptake was the same. Preincubation and incubation with 10 microM monensin (reduces the Na+ gradient) or 10 microM monodansylcadaverine (inhibits receptor-mediated endocytosis) reduced 15-min [125I] Triac uptake by 15% (P < 0.005) and 19% (P < 0.005), respectively. The data show that 1) Triac, on the basis of the free hormone concentration, is more potent than T3 or T4 in suppressing TSH secretion; and 2) the rapid uptake of [125I]Triac by the anterior pituitary occurs by a carrier-mediated mechanism that is only partially dependent on ATP or the Na+ gradient.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Everts ME,Visser TJ,Moerings EP,Docter R,van Toor H,Tempelaars AM,de Jong M,Krenning EP,Hennemann Gdoi
10.1210/endo.135.6.7988460subject
Has Abstractpub_date
1994-12-01 00:00:00pages
2700-7issue
6eissn
0013-7227issn
1945-7170journal_volume
135pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Tamoxifen, a partial competitive antagonist to the estrogen receptor, is a potent inhibitor of the proliferation of experimental mammary carcinoma in the rat and is widely used clinically in the treatment of breast cancer. Blockade of estrogen receptors present on neoplastic cells represents the classic mechanism of a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1350760
更新日期:1992-06-01 00:00:00
abstract::Human pituitary prolactin (hPRL) contains a single major component. Upon iodination by a lactoperoxidase procedure approximately 50% of this component remains ulaltered in molecular size and net charge by the criteria of quantitative polyacrylamide gel electrophoresis (PAGE). Aminiotic fluid hPRL contains two componen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-406
更新日期:1975-08-01 00:00:00
abstract::Hypoxia and biological responses to hypoxia are commonly encountered in both normal and pathologic cellular processes. Here we report that extracellular superoxide dismutase (EC-SOD) plays a major role in regulating the magnitude of hypoxia-induced erythropoietin (Epo) gene expression, thus implicating superoxide as a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1007
更新日期:2005-01-01 00:00:00
abstract::In previous studies we have shown that aromatase cytochrome P450 (P450arom) mRNA and protein increase markedly in luteal tissue between days 10-19 of gestation, whereas cholesterol side-chain cleavage cytochrome P450 (P450scc) appears to be constitutively maintained regardless of hormonal changes occurring during preg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1673
更新日期:1989-09-01 00:00:00
abstract::The epidermis has a great potential as a bioreactor to produce proteins with systemic action. However, the consequences of ectopic epidermal protein overexpression need to be carefully addressed to avoid both local and systemic adverse effects. Thus, the long-term effects of leptin on skin physiology have not been stu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0156
更新日期:2005-10-01 00:00:00
abstract::Hashimoto thyroiditis can be partially reproduced in mice by immunization with thyroglobulin or, more recently, thyroperoxidase. This experimental autoimmune thyroiditis (EAT) model has been extensively characterized during early disease phases (up to d 35 after immunization). By extending the analysis of EAT to 100 d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0639
更新日期:2009-01-01 00:00:00
abstract::Pituitary homeobox 1 (Ptx1) is a homeodomain-containing transcription factor acting on transcription of all pituitary hormone genes. Its expression is first detected in the stomodeal ectoderm and is maintained in all derivatives of this structure, including Rathke's pouch. We now show that Ptx1 is expressed in all pit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6549
更新日期:1999-03-01 00:00:00
abstract::Humanin (HN) is a novel 24-amino acid mitochondrial-derived peptide that has demonstrated diverse cytoprotective effects, including an emerging role in diabetes. The purpose of this study was to examine the pharmacokinetics of humanin analogues, which show great potential as therapeutic agents (HNG and the non-IGFBP-3...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2004
更新日期:2013-10-01 00:00:00
abstract::GnRH peptides in the hypothalami of marsupials (tammar wallaby, short-nosed bandicoot, and eastern quoll) and a monotreme (echidna) were investigated by reverse phase HPLC and RIA with region-specific antisera. In the wallaby hypothalamic extract, a single form of GnRH was present, which eluted in the same position as...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2244
更新日期:1989-11-01 00:00:00
abstract::We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as asses...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7545100
更新日期:1995-10-01 00:00:00
abstract::In the present study we have used a high affinity photoaffinity label (PAL) agonist (pGlu-His-Trp-Ser-125I-iodoTyr-D-Lys(para-N3-Benzoyl)-Leu-Arg-P roNHEt) and a PAL antagonist (NAcD2Nal-D4ClPhe-D3Pal-Ser-125I-iodo-NMeTyr-D-L ys(para-N3-Benzoyl)Leu-Lys(Isp) - Pro-D-Ala-NH2) to covalently label the GnRH receptor. Rat p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8393780
更新日期:1993-08-01 00:00:00
abstract::This review describes formation of the islet basement membrane and the function of extracellular matrix (ECM) components in β-cell proliferation and survival. Implications for islet transplantation are discussed. The insulin-producing β-cell is key for maintaining glucose homeostasis. The islet microenvironment greatl...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2019-00206
更新日期:2019-08-01 00:00:00
abstract::The regulation of cholecystokinin octapeptide (CCK-8) secretion was studied using a fetal cerebral cortical cell culture system. After 2-3 weeks in culture, the cells were utilized for short-term release experiments. CCK-8 was measured by RIA and its identity was confirmed by HPLC. Depolarization of the cells with K+ ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3125
更新日期:1991-12-01 00:00:00
abstract::Interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) are proinflammatory cytokines that affect the secretion of several neuroendocrine hormones. In addition, glial cells synthesize and release IL-6, suggesting a paracrine role for this cytokine in the brain. We have examined the regulation of IL-6 release from glial ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6536
更新日期:1999-02-01 00:00:00
abstract::We examined the effects of ACTH and the Ca++ ionophore, A23187, on steroidogenesis and phospholipid metabolism during incubation of dispersed rat adrenal cells. Increasing doses of ACTH elicited nearly parallel increases in corticosterone production and adrenal inositide (mono- and di-) concentrations. As reported pre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1243
更新日期:1981-04-01 00:00:00
abstract::The purpose of this investigation was to use an approach targeted specifically on endogenous relaxin to determine the influence of relaxin on birth in the rat. To that end, a monoclonal antibody specific for rat relaxin, designated MCA1, was used to passively neutralize endogenous relaxin in intact pregnant rats. MCA1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2479
更新日期:1988-11-01 00:00:00
abstract::TSH is the most important biomarker in the interpretation of thyroid function in man. Its levels are determined by circulating thyroid hormone (TH) levels that feed back centrally to regulate the expression of the subunits that comprise TSH from the pituitary. The nuclear corepressor 1 (NCoR1), is a critical coregulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1504
更新日期:2012-10-01 00:00:00
abstract::Leydig cells from mature rat testes contain high levels of 11 beta-hydroxysteroid dehydrogenase (11HSD), an enzyme that oxidatively inactivates glucocorticoids. We have proposed that the 11HSD of Leydig cells protects the testis from the effects of high levels of glucocorticoids, as may occur in stress and Cushing's d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119160
更新日期:1994-03-01 00:00:00
abstract::Acromegaly is associated with an increased incidence of cardiovascular disease. Transgenic mice expressing bovine GH (bGH) gene have previously been used to examine the effects of chronic GH stimulation on cardiovascular function. Results concerning systolic blood pressure (SBP) in bGH mice are conflicting. We hypothe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1899
更新日期:2014-03-01 00:00:00
abstract::Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. H...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1016
更新日期:2014-09-01 00:00:00
abstract::To investigate GH's role in diabetic end organ damage, experimental diabetes was induced with streptozotocin (STZ) in bovine GH (bGH) or bGH antagonist transgenic mice and in their nontransgenic (NTG) litter mates. Body growth, blood glucose, serum insulin-like growth factor-I levels, liver GH receptor (GHR) binding, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.2.7835300
更新日期:1995-02-01 00:00:00
abstract::The guinea pig is unusual in that its postnatal growth appears to be independent of GH even though its pituitary gland produces a GH molecule. The effects of fasting on the GH secretory pattern and the GH responses to insulin, GH-releasing factor (GRF), and somatostatin (SS) during fasting have now been studied by aut...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-716
更新日期:1990-08-01 00:00:00
abstract::The preoptic area (POA) of the male dove is a known target area for separable behavioral actions of testosterone and 17 beta-estradiol (E2) and contains an active aromatase system. We have examined the regulatory influence of gonadal hormones on aromatase using an in vitro microassay that measures conversion of [1 alp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2180
更新日期:1986-06-01 00:00:00
abstract::To mimick a state of hyperinsulinemia, normal rats were infused with insulin for 4 days via minipumps, and compared to saline infused rats. At the end of the experimental period, the abundance of mRNA was increased in white adipose tissue (WAT) and decreased in muscles of "insulinized" rats compared to controls. These...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3246
更新日期:1990-12-01 00:00:00
abstract::The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0349
更新日期:2005-08-01 00:00:00
abstract::Endoglin is an integral membrane glycoprotein predominantly expressed on human endothelial cells and recently shown to bind transforming growth factor-beta 1 (TGF beta 1) with high affinity. We now report the cloning and sequencing of a full-length murine endoglin complementary DNA of 2902 base pairs which hybridizes ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.6.8194490
更新日期:1994-06-01 00:00:00
abstract::The distribution and density of selectively labeled mu-, delta-, and kappa-opioid binding sites were examined by in vitro radioautography in the hypothalamus of normal, estradiol valerate (EV)-injected, and estradiol (E2)-implanted female rats. Hypothalamic beta-endorphin concentration was also examined by RIA in thes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-2969
更新日期:1990-12-01 00:00:00
abstract::Although acute leptin administration in the hypothalamus decreases food intake and increases peripheral energy metabolism, the peripheral actions of central chronic leptin administration are less understood. In this study, we investigated what effects chronic (7 d) intracerebroventricular (ICV) administration of lepti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1254
更新日期:2011-11-01 00:00:00
abstract::Gonadal differentiation is dependent upon a cascade of molecular and morphological events. Steroidogenic factor 1 (SF-1) and DAX-1 have been implicated in this process. A SF-1 binding site has been reported to be present on the DAX-1 gene. We therefore used immunocytochemistry to determine whether these two transcript...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754790
更新日期:1996-08-01 00:00:00
abstract::To determine the relative role of the androgenic and/or estrogenic components of the action of dehydroepiandrosterone (DHEA) on the histomorphology and structure of the rat mammary gland, ovariectomized (OVX) female animals received DHEA administered alone or in combination with the pure antiandrogen flutamide or the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5762
更新日期:1998-02-01 00:00:00