Abstract:
:Although acute leptin administration in the hypothalamus decreases food intake and increases peripheral energy metabolism, the peripheral actions of central chronic leptin administration are less understood. In this study, we investigated what effects chronic (7 d) intracerebroventricular (ICV) administration of leptin has on energy metabolism and insulin sensitivity in diet-induced obese mice. C57/BL mice were fed a low-fat diet (LFD; 10% total calories) or high-fat diet (HFD; 60% total calories) for 8 wk after which leptin was administered ICV for 7 consecutive days. Mice fed a HFD showed signs of insulin resistance, as evidenced by an impaired glucose tolerance test. Chronic leptin treatment resulted in a decrease in food intake and body weight and normalization of glucose clearance but no improvement in insulin sensitivity. Chronic ICV leptin increased hypothalamic signal transducer and activator of transcription-3 and AMP-activated protein kinase phosphorylation but did not change hypothalamic malonyl CoA levels in HFD fed and LFD-fed mice. In the gastrocnemius muscles, the levels of malonyl CoA in both leptin-treated groups were lower than their respective control groups, suggesting an increase in fatty acid oxidation. However, only in the muscles of ICV leptin-treated LFD mice was there a decrease in lipid metabolites including diacylglycerol, triacylglycerol, and ceramide. Our results suggest that chronic ICV leptin decreases food consumption and body weight via a mechanism different from acute ICV leptin administration. Although chronic ICV leptin treatment in HFD mice improves glucose tolerance, this occurs independent of changes in insulin sensitivity in the muscles of HFD mice.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Keung W,Palaniyappan A,Lopaschuk GDdoi
10.1210/en.2011-1254subject
Has Abstractpub_date
2011-11-01 00:00:00pages
4127-37issue
11eissn
0013-7227issn
1945-7170pii
en.2011-1254journal_volume
152pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Specific binding of human chorionic gonadotropin (hCG), and the hCG-sensitive adenyl cyclase of granulosa cells from small (1-2 mm), medium (3-5 mm), and large (6-12 mm) porcine ovarian follicles have been studied. The number of hCG-binding sites per cell (n) increases during follicle maturation without a change in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-1-42
更新日期:1976-07-01 00:00:00
abstract::Administration of ovine or rat PRL to animals, including man, has resulted in decreased urine volume and increased urine osmolality. Contamination of PRL preparations with vasopressin is the most likely explanation for the apparent antidiuretic effect. In this study, diabetes insipidus rats lacking vasopressin(homozyg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-4-1001
更新日期:1979-10-01 00:00:00
abstract::Activin A is a gonadal protein originally isolated from follicular fluid and is recognized as a local regulator of granulosa cell differentiation. Whether activin A promotes folliculogenesis, however, still remains unclarified. The present study was designed to elucidate the effect of activin A on follicular growth in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.11.5526
更新日期:1997-11-01 00:00:00
abstract::In healthy individuals, peripheral insulin resistance evoked by dietary saturated lipid can be accompanied by increased insulin secretion such that glucose tolerance is maintained. Substitution of long-chain omega-3 fatty acids for a small percentage of dietary saturated fat prevents insulin resistance in response to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0479
更新日期:2003-09-01 00:00:00
abstract::The objective of this study was to assess estrogen-dependent cellular mechanisms that could contribute to the acid pH of the vaginal lumen. Cultures of normal human cervical-vaginal epithelial (hECE) cells and endocervical cells were grown on filters, and acidification of the extracellular solutions on the luminal (L-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1153
更新日期:2005-02-01 00:00:00
abstract::Testosterone propionate causes a striking increase in in vivo uptake of 2-deoxyglucose by the levator ani muscle of immature male rats. Autoradiography showed the labeled 2-deoxyglucose to be uniformly distributed over the entire muscle. Liquid scintillation counting of whole muscles allowed quantification of 2-deoxyg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-119
更新日期:1983-07-01 00:00:00
abstract::The effects of L-leucine, D-leucine, and L-isoleucine upon the secretion of glucagon and insulin were investigated using the isolated, perfused rat pancreas. All experiments were conducted in the presence of 5.6 mM D-glucose. Ten-minute perfusions of 2, 5, and 10 mM L-leucine induced the release of glucagon and insuli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1208
更新日期:1978-10-01 00:00:00
abstract::Thyroid dysfunction during human pregnancy is closely related to serious pregnancy outcome. However, the regulation and function of thyroid hormones during early pregnancy are largely unknown. We found that type II deiodinase, an enzyme converting T4 to activated T3, is highly expressed in the mouse uterus on days 3 a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1015
更新日期:2014-07-01 00:00:00
abstract::Insulin coordinates the complex response to feeding, affecting numerous metabolic and hormonal pathways. Forkhead box protein O1 (FoxO1) is one of several signaling molecules downstream of insulin; FoxO1 drives gluconeogenesis and is suppressed by insulin. To determine the role of FoxO1 in mediating other actions of i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00870
更新日期:2018-03-01 00:00:00
abstract::Ionic mechanisms play an important role in the regulation of hormone secretion. The GHRH-induced GH release by human GH-secreting cells is transmitted through protein kinase A (PKA), which activates nonselective cation current (NSCC) and induces membrane depolarization, intracellular Ca2+ increase, and GH secretion. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6731
更新日期:1999-05-01 00:00:00
abstract::Prostaglandin E2 (PGE2) stimulates the formation of osteoclast-like tartrate-resistant acid phosphatase-positive multinucleated cells (TRAP + MNC) in vitro. This effect likely results from stimulation of adenylyl cyclase, which is mediated by two PGE2 receptors, designated EP2 and EP4. We used cells from mice in which...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.6.7518
更新日期:2000-06-01 00:00:00
abstract::A rare 20K isoform of GH-V (here abbreviated as GHv) was discovered in 1998. To date, only 1 research article has characterized this isoform in vivo, observing that GHv treatment in male high-fat fed rats had several GH-like activities, but unlike GH lacked diabetogenic and lactogenic activities and failed to increase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa097
更新日期:2020-08-01 00:00:00
abstract::Previous studies have demonstrated that uterine progesterone (P) receptor is under dual hormonal control; estradiol (E2) induces the synthesis of cytosolic P receptor, and P induces the loss of its own receptor and antagonizes E2-induced P receptor replenishment. The objective of this study was to examine the contribu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2488
更新日期:1986-06-01 00:00:00
abstract::GnRH neurons are the central regulators of fertility, and their activity is modulated by steroid feedback. In women with hyperandrogenemic infertility and in animal models of these disorders, elevated androgen levels interfere with progesterone (P) negative feedback. Our previous work showed that steroids altered the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1029
更新日期:2006-03-01 00:00:00
abstract::Immune activation results in the activation of adrenal steroidogenesis and inhibition of gonadal steroidogenesis. Previous studies indicated that these effects were caused primarily by activation and suppression of the secretion of ACTH and LH, respectively. However, other evidence indicated a direct effect of the imm...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7780
更新日期:2000-11-01 00:00:00
abstract::The uptake of [125I]T3 in rat skeletal muscle was investigated by incubating intact soleus muscles with a tracer amount of [125I]T3. At 37 C [125I]T3 uptake increased asymptotically; at 60 min the muscle contained 10% of the total [125I]T3 or 0.238 +/- 0.021% per mg wet tissue. At 0 C the [125I]T3 uptake was 1/5 of th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2755
更新日期:1986-12-01 00:00:00
abstract::To identify factors that regulate the tempo of growth and puberty, the present study examined how the environment influenced the timing of menarche and first ovulation in rhesus monkeys and how these events were related to differential rates of growth. Spring-born females were raised from 12 months of age under natura...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2653
更新日期:1988-12-01 00:00:00
abstract::The contributions of estradiol and testosterone to atherosclerotic lesion progression are not entirely understood. Cross-sex hormone therapy (XHT) for transgender individuals dramatically alters estrogen and testosterone levels and consequently could have widespread consequences for cardiovascular health. Yet, no prec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00884
更新日期:2018-02-01 00:00:00
abstract::Homeostatic control of endocrine systems proceeds via feedforward (agonistic, stimulatory) and feedback (antagonistic, inhibitory) interactions mediated via implicit dose-response functions. However, neither the feedback/feedforward pathways nor the dose-response interfaces are directly observed in vivo. Thus, the goa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1461
更新日期:2011-12-01 00:00:00
abstract::The CRH-binding protein (CRH-BP) antagonizes the ACTH-releasing activity of the neuropeptide CRH in vitro. However, the function of CRH-BP in vivo and the molecular mechanisms that regulate CRH-BP expression are not well understood. In this study, the rat CRH-BP gene was characterized, and CRH-BP promoter sequences we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5128
更新日期:1997-05-01 00:00:00
abstract::The microenvironment of placental mesenchymal stem cells (PMSCs) is dynamic throughout gestation and determines changes in cell fate. In vivo, PMSCs initially develop in low-oxygen tension and low IGF-I concentrations, and both increase gradually with gestation. The impact of varying concentrations of IGF-I and changi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1842
更新日期:2014-04-01 00:00:00
abstract::Glucose homeostasis depends on the coordinated secretion of glucagon, insulin, and Glucagon-like peptide (GLP)-1 by pancreas and intestine. Obesity, which is associated with an increased risk of developing insulin resistance and type 2 diabetes, affects the function of these organs. Here, we investigate the functional...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1433
更新日期:2016-10-01 00:00:00
abstract::Patients with Graves' disease and exophthalmos demonstrate delayed hypersensitivity to antigens present in extracts of certain normal human tissue; namely,thyroid gland and retroorbital tissue. The delayed hypersensitivity can be assayed in vitro by quantitating the amount of a lymphokine, migration inhibition factor ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-351
更新日期:1977-02-01 00:00:00
abstract::The insulin-like growth factors (IGF)-I and -II are bound to specific carrier proteins in the circulation. For investigation of their physiological role, the acid-stable subunit of the major binding protein (SmBP) was isolated from human plasma Cohn fraction IV. Its effect on the mitogenic activity of IGF-I was studie...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-766
更新日期:1989-08-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::Insulin-like growth factor-binding protein-3 (IGFBP-3), the major IGFBP in the adult circulation, is produced by a wide range of cell and tissue types. IGFBP-3 appears to be regulated by transcriptional and/or posttranslational mechanisms in a species-, cell-, and development-specific manner. In vitro and in vivo stud...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.2.7530650
更新日期:1995-02-01 00:00:00
abstract::Steady state levels of plasma membrane receptors (measured in radioligand assays) result from processes that contribute to the production of receptors (synthesis, recycling, and unmasking) as well as those that contribute to the loss of receptors (degradation, internalization, and inactivation). Accordingly, we have p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2577
更新日期:1990-05-01 00:00:00
abstract::We have recently identified gonadotropes as target cells for ATP action via ATP receptors of the P2U subtype. The present studies have used gonadotrope-derived alpha T3-1 cells to examine the possible signaling mechanisms subserving ATP action in gonadotropes. Addition of ATP produced a biphasic intracellular Ca2+ (Ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536620
更新日期:1996-01-01 00:00:00
abstract::Basal serum gonadotropin levels were monitored weekly in female hamsters from birth to 10 weeks of age. Hamsters raised on three different photoperiods presented uniform pre- and postpubertal patterns of serum LH and FSH, suggesting that gonadotropin release in the young hamster occurs independently of ambient photope...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1262
更新日期:1982-04-01 00:00:00
abstract::To determine if CRH and glucocorticoids are respectively required for hypophagia and catabolism in malnutrition, we have subjected wild-type (WT) and CRH knockout (KO) mice to dietary protein deprivation. Compared with WT mice, CRH KO mice exhibited greater decreases in food intake and negligible change in plasma cort...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6910
更新日期:1999-08-01 00:00:00