Abstract:
:The uptake of [125I]T3 in rat skeletal muscle was investigated by incubating intact soleus muscles with a tracer amount of [125I]T3. At 37 C [125I]T3 uptake increased asymptotically; at 60 min the muscle contained 10% of the total [125I]T3 or 0.238 +/- 0.021% per mg wet tissue. At 0 C the [125I]T3 uptake was 1/5 of that at 37 C. The specific [125I]T3 uptake, determined by subtracting the uptake in the presence of 10 microM unlabeled T3 from the total [125I]T3 uptake, attained a plateau after 60 min. Washout experiments, done by first incubating the muscle for 60 min at 37 C or 0 C with [125I]T3 and then at 0 C for 3 h with unlabeled T3, showed that 21 +/- 2% or 58 +/- 4% of the radioactivity, respectively, was released, indicating an intracellular location of the hormone after incubation at 37 C. Addition of increasing concentrations of L-T3, D-T3 and L-T4 caused a progressive inhibition of the [125I]T3 uptake; the 50% inhibitory concentrations being 400 nM, 7 microM, and more than 15 microM, respectively. Preincubation of soleus muscles with metabolic inhibitors almost completely inhibited [125I]T3 specific uptake, with oligomycin and antimycin causing 98 +/- 4% and 81 +/- 3% reduction, respectively. Monodansylcadaverine and bacitracin, inhibitors of receptor-mediated endocytosis, reduced the specific [125I]T3 uptake in a dose-dependent manner up to 67 +/- 3% and 62 +/- 2%, respectively. These results indicate the presence of a saturable, stereospecific, and energy-dependent process responsible, at least in part, for T3 uptake in rat skeletal muscle. This specific T3 uptake may be a receptor-mediated endocytosis process.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Pontecorvi A,Robbins Jdoi
10.1210/endo-119-6-2755subject
Has Abstractpub_date
1986-12-01 00:00:00pages
2755-61issue
6eissn
0013-7227issn
1945-7170journal_volume
119pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Hyperaldosteronism is linked to the development and progression of several different cardiovascular diseases. Angiotensin (Ang) II increases aldosterone secretion and adrenal blood flow. Ang II peptide fragments are produced by various peptidases, and these Angs have diverse and vital physiologic roles. Due to the unc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1160
更新日期:2013-12-01 00:00:00
abstract::UMR106-06 cells predominantly express the C1a isoform of the rat calcitonin (CT) receptor (CTR). We have compared the homologous regulation of the C1a CTR endogenously expressed in UMR106-06 cells with the cloned C1a CTR in transfected HEK 293 cells, in which expression is driven by a heterologous promoter. It was fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895320
更新日期:1996-11-01 00:00:00
abstract::Chronically elevated fatty acids contribute to insulin resistance through poorly defined mechanisms. Endoplasmic reticulum (ER) stress and the subsequent unfolded protein response (UPR) have been implicated in lipid-induced insulin resistance. However, the UPR is also a fundamental mechanism required for cell adaptati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1881
更新日期:2012-05-01 00:00:00
abstract::Insulin-like growth factors (IGFs), when isolated from serum or tissue fluids, are usually found as part of a protein complex which also contains one of several IGF binding proteins (IGFBPs). Although some IGFBPs have been shown to alter interactions of IGFs with their receptors in vitro and can modify the responses o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7507840
更新日期:1994-02-01 00:00:00
abstract::Differentiation of primary villous cytotrophoblasts into syncytia is associated with increasing production of alpha and beta human CG subunits, which is predominantly governed at the level of messenger RNA expression. Here, we present a detailed study on the mechanisms involved in the differentiation-dependent regulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.10.7713
更新日期:2000-10-01 00:00:00
abstract::We have previously shown that placental protein 12 (PP12) is synthesized and secreted by human term pregnancy decidua in vitro. In the present study, fragments of proliferative and secretory phase endometrium were cultured in media in the presence and absence of progesterone (P) and 17 beta-estradiol (E2) for 96 h. Th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-1067
更新日期:1986-03-01 00:00:00
abstract::N-methyl-D,L,-aspartate (NMA), an analog of the excitatory neurotransmitter aspartate, has been previously shown to acutely stimulate LH release in the rodent and primate. In this study, we examine the effect of NMA on LH secretion in the long term ovariectomized adult rhesus monkey. After a 3-h control period, three ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-724
更新日期:1990-08-01 00:00:00
abstract::The human breast cancer cell line MCF-7 responds to estrogens with increased progesterone receptor (PR) levels. In this study, we use dense amino acid density shift analyses to address directly the question of whether estrogen increases PR levels in MCF-7 cells by altering rates of receptor synthesis and/or degradatio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-3-935
更新日期:1988-03-01 00:00:00
abstract::Increasing adiposity predisposes to the development of the metabolic syndrome, in part, through adipose tissue dysregulation and inflammation. In addition, offspring nutrient-restricted (NR) in utero can exhibit an increased risk of early-onset insulin resistance and obesity, although the mechanisms remain unclear. We...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1784
更新日期:2009-08-01 00:00:00
abstract::In vivo and in vitro evidence indicates that FSH is a primary regulator of testicular inhibin production. However, recent reports suggest that LH may also promote inhibin secretion in vivo. To investigate whether LH regulates inhibin subunit messenger RNA (mRNA) expression as well, we examined the effects of hypophyse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1097
更新日期:1990-09-01 00:00:00
abstract::We have shown previously that neonatal testicular gonocytes express platelet-derived growth factor receptors (PDGFR) alpha and beta. We report the expression of a novel PDGFRbeta (V1-PDGFRbeta) transcript in gonocytes of 3-d-old rat testes. V1-PDGFRbeta nucleotide sequence spans from intron 6 to exon 23 of the PDGFRbe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1206
更新日期:2007-05-01 00:00:00
abstract::Atrial, brain-type, and C-type natriuretic peptides (ANP, BNP, and CNP) act via receptors with intrinsic guanylate cyclase activity. The A-type and B-type ANP receptors are selectively activated by ANP and CNP, respectively. The anterior pituitary is a site of ANP production and action, suggesting a local regulatory f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.5.7682940
更新日期:1993-05-01 00:00:00
abstract::Prolonged stimulation of cells by beta-adrenergic receptor agonists may lead to diminished responsiveness of the cells to subsequent activation by catecholamines. This phenomenon has been termed desensitization; the mechanism(s) for desensitization may involve an apparent loss in the number of beta-adrenergic receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1272
更新日期:1984-04-01 00:00:00
abstract::A clonal cell line (44-2C) which synthesizes and secretes somatostatin, neurotensin, calcitonin (CT), and CT gene-related peptide and transiently expresses c-fos was used to characterize the mechanism of action of basic fibroblast growth factor (bFGF). bFGF had two modes of action: 1) short term incubation of 44-2C ce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-3-1121
更新日期:1988-03-01 00:00:00
abstract::Androgens act to stimulate spermatogenesis through androgen receptors (ARs) on the Sertoli cells and peritubular myoid cells. Specific ablation of the AR in either cell type will cause a severe disruption of spermatogenesis. To determine whether androgens can stimulate spermatogenesis through direct action on the peri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1333
更新日期:2010-05-01 00:00:00
abstract::Task3 K(+) channels are highly expressed in the adrenal cortex and contribute to the angiotensin II and K(+) sensitivity of aldosterone-producing glomerulosa cells. Adult Task3(-/-) mice display a partially autonomous aldosterone secretion, subclinical hyperaldosteronism, and salt-sensitive hypertension. Here, we inve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1101
更新日期:2013-08-01 00:00:00
abstract::The direct effects of epinephrine and norepinephrine (NE) on lipid mobilization were studied in coho salmon liver slices incubated in vitro. Fatty acid (FA) release from liver slices was measured continuously by pH-stat titration. The pH-stat assay system was validated by simultaneous colorimetric measurement of FA an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2234
更新日期:1987-06-01 00:00:00
abstract::Thyroid dysfunction during human pregnancy is closely related to serious pregnancy outcome. However, the regulation and function of thyroid hormones during early pregnancy are largely unknown. We found that type II deiodinase, an enzyme converting T4 to activated T3, is highly expressed in the mouse uterus on days 3 a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1015
更新日期:2014-07-01 00:00:00
abstract::We have examined expression of the chicken PRL receptor (cPRLR) gene in different tissues of the chicken by Northern blot analysis. Most tissues examined (ovary, testis, oviduct, kidney, and fat) possess a prominent full-length (4.6-kb) cPRLR transcript. A larger (11.7-kb) transcript is also detected in ovary, oviduct...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6603
更新日期:1999-03-01 00:00:00
abstract::GH3 rat pituitary cells have L-type voltage-gated calcium channels, and both the rate of uptake of 45Ca2+ and the concentration of intracellular free calcium ion ([Ca2+]i) are increased by depolarization with high potassium. Cells incubated for several days with 10 nM epidermal growth factor (EGF) responded to depolar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.7686480
更新日期:1993-07-01 00:00:00
abstract::The low-density lipoprotein receptor (Ldlr) is a key molecule involved with lipid clearance. The Ldlr(-/-) mouse has been used extensively as a model for studying atherosclerosis. This study sought to characterize the energy balance phenotype of Ldlr(-/-) mice with respect to weight gain, body composition, energy expe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0496
更新日期:2010-11-01 00:00:00
abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-2014
更新日期:1981-05-01 00:00:00
abstract::Liver receptor homolog-1 (LRH-1) is an orphan nuclear receptor that belongs to the NR5A subgroup of nuclear receptors. LRH-1 induces key genes to regulate metabolic process, ovarian function, cancer cell proliferation, and steroidogenesis. In the breast, LRH-1 modulates and synergizes with endogenous estrogen signalin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1948
更新日期:2014-05-01 00:00:00
abstract::Repeated injections of recombinant human (rh) activin-A over a 2- to 3-day period are reportedly needed to stimulate the in vivo secretion of FSH. In this paper we present results showing that acute treatment with rh-activin-A caused marked and dose-dependent increases in plasma FSH, but not LH, levels in adult female...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2463
更新日期:1991-11-01 00:00:00
abstract::We have previously described FSH receptor-mediated influx of 45Ca++ in cultured Sertoli cells from immature rats and receptor-enriched proteoliposomes via activation of voltage-sensitive and voltage-independent calcium channels. We have further shown that this effect of FSH does not require cholera toxin- or pertussis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-2745
更新日期:1991-06-01 00:00:00
abstract::Ghrelin is produced by the gastrointestinal tract, and its systemic concentrations are mainly regulated by nutritional factors. Our aim was to investigate: 1) endogenous portal and systemic acylated and unacylated ghrelin levels (AG and UAG, respectively); 2) whether an iv glucose tolerance test (IVGTT) modifies AG an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0225
更新日期:2007-11-01 00:00:00
abstract::Circulating insulin-like growth factor (IGF) bioactivity is reduced in animals and patients with diabetes mellitus. We sought to determine whether the availability and levels of specific IGF binding proteins (BPs) are altered in animals with experimental diabetes, and might contribute to changes in circulating IGF bio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2614
更新日期:1990-05-01 00:00:00
abstract::Corticostatin-I (CSI) is the most potent of the corticostatic peptides isolated from rabbit lungs and neutrophils. CSI was localized in cells by immunohistochemistry using highly specific polyclonal antisera raised against the synthetic peptide. Western blot showed a single 4-kilodalton band, indicating that the antib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8504740
更新日期:1993-06-01 00:00:00
abstract::It is unclear whether the maturation of corticotrophs from the fetal to the adult type in the fetal sheep pituitary in late gestation is associated with changes in the sensitivity of the fetal pituitary to corticotrophic secretagogues and in the form of ACTH-containing peptides (IR-ACTH) secreted into the circulation....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1942
更新日期:1991-10-01 00:00:00
abstract::The present studies examine the initial events in the action of PTH, namely receptor binding and adenylate cyclase activation in the postobstructed canine kidney. Both kidneys were removed from five mongrel dogs 2 h after relief of unilateral ureteral obstruction (UUO) of 42 h duration. Basolateral membranes (BLM) wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1060
更新日期:1985-03-01 00:00:00