Abstract:
:We have examined expression of the chicken PRL receptor (cPRLR) gene in different tissues of the chicken by Northern blot analysis. Most tissues examined (ovary, testis, oviduct, kidney, and fat) possess a prominent full-length (4.6-kb) cPRLR transcript. A larger (11.7-kb) transcript is also detected in ovary, oviduct, testis, and kidney after longer exposure. A unique pattern of cPRLR expression was found in the testis of sexually mature chickens, which have an unusually high abundance of three small transcripts (1.2, 1.7, and 2 kb) in addition to the 4.6-kb transcript found in other tissues. Three domain-specific complementary DNA (cDNA) probes were constructed that correspond to the first and second ligand-binding regions in the extracellular domain and the transmembrane-intracellular domain. With these probes, Northern blot analysis of polyadenylated RNA prepared from the testes of a mature (22-week-old) chicken indicates that the highly abundant (1.2- and 1.7-kb) and less abundant (2.0-kb) cPRLR transcripts in testis hybridize only to the intracellular domain probe. Two types of truncated testis-specific cPRLR transcripts were identified using 5'-RACE (rapid amplification of cDNA ends) analysis of polyadenylated RNA from the testis of a 22-week-old chicken. The predominant truncated cDNA sequence contains the highly conserved box 1 motif [(+)box 1 cDNA] and diverges (at nucleotide 1396) from that of the cPRLR cDNA, just downstream of the transmembrane domain. The other truncated cDNA lacks the box 1 motif [(-)box 1 cDNA], which is replaced by 39 bases that could encode a hydrophobic N-terminus with a putative 5'-untranslated region of 131 bases. Young chickens predominately express the full-length cPRLR messenger RNA (4.6 kb) in the testis. At the onset of sexual maturity, there is a dramatic increase in abundance of the testis-specific (+)box 1 transcript, whereas expression of the full-length cPRLR is depressed. The presence of truncated [(+) or (-)box 1] cPRLR transcripts in the sexually mature chicken testis suggests a complex mechanism of PRL action on gonadal function.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Mao JN,Burnside J,Li L,Tang J,Davolos C,Cogburn LAdoi
10.1210/endo.140.3.6603subject
Has Abstractpub_date
1999-03-01 00:00:00pages
1165-74issue
3eissn
0013-7227issn
1945-7170journal_volume
140pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The occupancy of atrial natriuretic peptide (ANP) receptors of the ANPA type in human neuroblastoma NB-OK-1 cells elevates cGMP. In this study, ANP concentrations of 10 nM or more increased total K+ uptake. Data obtained in the presence of bumetanide and/or ouabain demonstrated that 1 microM ANP induced a primary stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8391430
更新日期:1993-07-01 00:00:00
abstract::Experiments were conducted to investigate the actions of glucocorticoids and insulin on the induction of a specific mRNA (p33-mRNA) expressed in rat H4 hepatoma cells. Previous studies have found a 10-fold increase in this mRNA following 60 min of insulin addition. In the present study, dexamethasone (Dex) induced the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-754
更新日期:1989-02-01 00:00:00
abstract::The potential role of basic fibroblast growth factor (bFGF) as a mediator of cell-cell interactions in the growth and development of the testis was examined. Nuclease protection analysis was used to evaluate bFGF gene expression in the testis and other male reproductive tract tissues. bFGF expression was evident in se...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446630
更新日期:1992-12-01 00:00:00
abstract::PTH stimulates synthesis and secretion of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in renal proximal tubule cells through activation of the protein kinase-A (PKA) or the protein kinase-C (PKC) signaling pathway. The relative contribution of the two transducing systems was explored using PTH fragments with selective act...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8344210
更新日期:1993-08-01 00:00:00
abstract::Kisspeptins, products of the KiSS-1 gene with ability to bind G protein-coupled receptor 54 (GPR54), have been recently identified as major gatekeepers of reproductive function with ability to potently activate the GnRH/LH axis. Yet, despite the diversity of functional states of the female gonadotropic axis, pharmacol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1463
更新日期:2006-06-01 00:00:00
abstract::Pulsatile administration of glucagon to perifused rat hepatocytes stimulates hepatocyte glucose production (HGP) more effectively than continuous administration. Having established that this effect was due to delayed relaxation of glucagon-stimulated HGP (t1/2 for decay = 3.54 +/- 0.60 min) we wished to examine the me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-732
更新日期:1987-08-01 00:00:00
abstract::IGF-I has been shown to play a role in the progression of atherosclerosis in experimental animal models. IGF-binding protein-4 (IGFBP-4) binds to IGF-I and prevents its association with receptors. Overexpression of a protease-resistant form of IGFBP-4 has been shown to inhibit the ability of IGF-I to stimulate normal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0571
更新日期:2007-10-01 00:00:00
abstract::The role of GH during the ovine estrous cycle is unknown. In this study, pituitary GH mRNA and peripheral serum GH levels were measured in ewes representing the major phases of the estrous cycle. There was a significant rise (P less than 0.05) in both GH mRNA and serum GH concentrations during the follicular phase of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1474
更新日期:1989-09-01 00:00:00
abstract::Angiotensin-II, the most important biologically active product of the renin-angiotensin system, has been reported to play a role in neovascularization, and prorenin has been found in the vitreous of human eyes, particularly in those affected by proliferative diabetic retinopathy, a disease characterized by neovascular...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1673
更新日期:1990-03-01 00:00:00
abstract::The direct actions of kit-ligand/stem cell factor (KL) in developing ovarian follicles were investigated. Previous studies have shown that granulosa cells express KL that can support oocyte development. The current study demonstrates that KL can also act directly on theca cells to promote cellular growth and different...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5368
更新日期:1997-09-01 00:00:00
abstract::The circadian rhythm of blood corticosterone was examined in rats whose feeding was restricted to a specific time of day. The peak of the corticosterone level was found just before feeding time regardless of whether, the rats were kept on a 12-h light, 12-h dark cycle or in constant light or were blinded. It took at l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-3-918
更新日期:1980-03-01 00:00:00
abstract::It has previously only been possible to assess osteoclastic bone resorption in intact bone, where other cell types may modify or mediate osteoclastic responses to environmental agents. We have recently developed techniques which enable us to measure bone resorption by osteoclasts extracted from bone and have used thes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-234
更新日期:1985-01-01 00:00:00
abstract::Hypoglycemia associated with diabetes management is a potential risk for cardiovascular diseases. However, the effect of hypoglycemic episodes including a surge of sympathetic activity on the progression of neointima formation after vascular injury remains largely unknown. In this study, insulin was injected intraperi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1628
更新日期:2014-06-01 00:00:00
abstract::A member of the interferon-inducible p200 family of proteins, p204, has recently been reported to function in the development of many mesoderm-derived tissues, such as bone, muscle, and cartilage. However, no published study has yet investigated the role of p204 in adipogenesis. Our preliminary experiments showed that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1381
更新日期:2010-07-01 00:00:00
abstract::Angiotensin II (AngII) stimulates aldosterone biosynthesis in the zona glomerulosa of the adrenal cortex. AngII also triggers the MAPK pathways (ERK1/2 and p38). Because ERK1/2 phosphorylation is a transient process, phosphatases could play a crucial role in the acute steroidogenic response. Here we show that the dual...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0241
更新日期:2007-11-01 00:00:00
abstract::The present studies were designed to evaluate the binding characteristics of arginine vasopressin (AVP), using rat anterior pituitary dispersed cells, in correlation with the biological activity of the peptide. Synthetic AVP released ACTH from dispersed anterior pituitary cells in a concentration-dependent fashion, wi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-6-2247
更新日期:1984-06-01 00:00:00
abstract::Livers from fed rats (180-240 g) were perfused noncyclically with a hemoglobin-free medium in vitro to determine whether vasoactive intestinal peptide (VIP) increases hepatic glucose production through a cAMP- or a Ca(2+)-dependent mechanism. Glucose output did not increase, but cAMP increased maximally during 10(-9) ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1372240
更新日期:1992-04-01 00:00:00
abstract::To understand the physiological role of ghrelin, it is crucial to study both the actions of ghrelin and the regulation of ghrelin secretion. Although ghrelin actions have been extensively revealed, the direct factors regulating ghrelin secretion by ghrelin-producing cells (X/A-like cells), however, is not fully unders...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1455
更新日期:2011-07-01 00:00:00
abstract::Angiotensin II and extracellular potassium stimulate aldosterone production in adrenal glomerulosa cells by mobilizing the calcium messenger system. This response requires calcium influx across the plasma membrane, followed by calcium uptake into the mitochondria. It has been proposed that calcium is transported to th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0268
更新日期:2003-10-01 00:00:00
abstract::The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1168
更新日期:1985-03-01 00:00:00
abstract::Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0826
更新日期:2003-12-01 00:00:00
abstract::Recent observations suggest a role for interleukin-1 (IL-1), a polypeptide product of macrophage/monocytic cells, in the immune-mediated destruction of pancreatic islet beta-cells observed in type 1 diabetes. In this study, we investigated the effects of IL-1 on both alpha- and beta-cell secretory functions in rat isl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2393
更新日期:1988-06-01 00:00:00
abstract::We recently identified a 32 K mol wt insulin-like growth factor (IGF)-binding protein (BP) which is markedly increased in the serum of streptozotocin-diabetic rats and recognized by antiserum against the human amniotic fluid IGFBP (hIGFBP-1). In the present study we sought to confirm that this protein represents the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-789
更新日期:1990-08-01 00:00:00
abstract::3-Iodothyronamine (3-T 1 AM) and thyronamine (T AM) are novel endogenous signaling molecules that exhibit great structural similarity to thyroid hormones but apparently antagonize classical thyroid hormone (T(3)) actions. Their proposed biosynthesis from thyroid hormones would require decarboxylation and more or less ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1678
更新日期:2008-06-01 00:00:00
abstract::Maternal and fetal metabolism of vitamin D was examined in term pregnant rabbits fed a normal diet and in those supplemented with a large amount of vitamin D3. Term pregnant rabbits (27--30 days of gestation) fed the normal diet showed lower levels of plasma calcium, 25-hydroxyvitamin D3 (250HD3), and 24,25-dihydroxyv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-1950
更新日期:1982-06-01 00:00:00
abstract::For centuries our image of the skeleton has been one of an inert structure playing a supporting role for muscles and a protective role for inner organs like the brain. Cell biology and physiology modified this view in the 20st century by defining the constant interplay between bone-forming and bone resorbing cells tha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa149
更新日期:2020-10-01 00:00:00
abstract::We have investigated the interaction between hypothalamic ACTH secretagogues and adrenocortical glucocorticoids in rat anterior pituitary tissue using an in vitro perifusion system. Repeated 5 min pulses of 41-residue CRF (CRF-41) or arginine vasopressin (AVP) were applied at 1 h intervals for up to 7 h. Administratio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1312450
更新日期:1992-04-01 00:00:00
abstract::Pancreatic membrane receptors for cholecystokinin (CCK) in obese and nonobese Zucker rats were compared with the use of a biologically active [125I]iodo-CCK-8 radioprobe. Membrane homogenates from obese rats bound half the amount of radioligand in 2 h as did membranes from lean rats (specifically bound, 7.0% vs. 14.0%...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-546
更新日期:1986-08-01 00:00:00
abstract::GnRH, the main regulator of reproduction, is produced in a variety of tissues outside of the hypothalamus, its main site of synthesis and release. We aimed to determine whether GnRH produced in the female rat pituitary and ovaries is involved in the processes leading to ovulation. We studied the expression patterns of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0240
更新日期:2005-08-01 00:00:00
abstract::Bone remodeling is a complex process of coordinated resorption and formation of bone, which is regulated by systemic hormones and by local factors. We have previously shown that the peptide hormone adrenomedullin is mitogenic to osteoblastic cells in vitro and that it promotes bone growth in vivo. The aim of the prese...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8152
更新日期:2001-05-01 00:00:00