Abstract:
:The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucose concentration (3.3 mmol/liter), leucine caused a dose-related biphasic increase in glucagon output in the absence of arginine, but only a transient increase in the presence of the latter amino acid. These positive responses were markedly reduced and, on occasion, abolished at a high glucose concentration (8.3 mmol/liter). Moreover, at a low glucose concentration (3.3 mmol/liter) and in the presence of arginine, the highest concentration of leucine (15.0 mmol/liter) provoked a sustained and reversible inhibition of glucagon release. Likewise, leucine (15.0 mmol/liter) reversibly inhibited glucagon secretion evoked by glutamine in the absence of glucose. Thus, leucine exerted a dual effect on the secretion of glucagon, the inhibitory effect of leucine prevailing at a high concentration of the branched chain amino acid and when glucagon secretion was already stimulated by arginine or glutamine. At a physiological concentration (0.2 mmol/liter), however, leucine was a positive stimulus for glucagon release, especially in the absence of another amino acid. Concomitantly, leucine was always a positive stimulus for both insulin and somatostatin secretion. The intimate mechanisms involved in the dual effect of leucine on glucagon secretion remain to be elucidated.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Leclercq-Meyer V,Marchand J,Woussen-Colle MC,Giroix MH,Malaisse WJdoi
10.1210/endo-116-3-1168subject
Has Abstractpub_date
1985-03-01 00:00:00pages
1168-74issue
3eissn
0013-7227issn
1945-7170journal_volume
116pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The uterotropic response of the uterus to 17β-estradiol (E2) is genetically controlled, with marked variation observed depending on the mouse strain studied. Previous genetic studies from our laboratory using inbred mice that are high (C57BL6/J; B6) or low (C3H/HeJ; C3H) responders to E2 led to the identification of q...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1910
更新日期:2014-08-01 00:00:00
abstract::Proteolytic degradation of extracellular matrix components has been suggested to play an essential role in the occurrence of ovulation. Recent studies in our laboratory have indicated that the plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are no...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1736
更新日期:2008-10-01 00:00:00
abstract::The binding of oxytocin (OT) to receptors in rabbit amnion cells stimulates PGE2 release. We previously studied the binding characteristics, changes in receptor concentration during pregnancy, and agonist specificity of OT action on amnion cells. In this study the molecular size of OT receptors in rabbit amnion was es...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1317790
更新日期:1992-06-01 00:00:00
abstract::The purpose of this study was to determine whether normal morphological development occurs in pituitary corticotrophs deprived of products of the hypothalamic paraventricular nucleus (PVN), e.g. corticotropin releasing hormone and arginine vasopressin (AVP), after PVN lesions. In addition, we have attempted to ascerta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.3.1324150
更新日期:1992-09-01 00:00:00
abstract::The crystal structure of human CG reveals that each subunit is a member of the superfamily of cystine-knot growth factors. Although the distribution of the cysteine residues in all the beta-subunits is conserved, the conformation of the human FSH dimer differs from that of the CG/LH dimers. This suggests that the func...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7821
更新日期:2000-12-01 00:00:00
abstract::Activators of protein kinase C, such as phorbol myristate acetate (PMA) and the synthetic diacylglycerol dioctanoylglycerol (diC8), either stimulate or inhibit PTH release depending on the extracellular Ca2+ concentration. By increasing PTH release at high extracellular Ca2+, these agents, in effect, block high Ca2(+)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-1-141
更新日期:1990-07-01 00:00:00
abstract::Poor growth before birth is associated with impaired insulin sensitivity later in life, increasing the risk of type 2 diabetes. The tissue sites at which insulin resistance first develops after intrauterine growth restriction (IUGR), and its molecular basis, are unclear. We have therefore characterized the effects of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1955
更新日期:2012-05-01 00:00:00
abstract::In agonadal juvenile male monkeys, continuous administration of human metastin 45-54 (hu metastin 45-54) leads to desensitization of its receptor, G protein-coupled receptor 54 (GPR54), and decreased LH. The present study extended this observation to the adult male monkey, a more preclinically relevant model in which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0207
更新日期:2007-07-01 00:00:00
abstract::Basal growth hormone (GH) levels are higher in female than male primates, and estradiol (E) treatment of gonadectomized primates increases serum GH. To determine if the effect of E is mediated at the level of the somatotroph, we verified the effect of E-treatment on serum GH in spayed macaques, and then examined the e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-2110
更新日期:1991-10-01 00:00:00
abstract::The endoplasmic reticulum (ER) mediates the first steps of protein assembly within the secretory pathway and is the site where protein folding and quality control are initiated. The storage and release of Ca2+ are critical physiological functions of the ER. Disrupted ER homeostasis activates the unfolded protein respo...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqz028
更新日期:2020-02-01 00:00:00
abstract::The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-178
更新日期:1983-07-01 00:00:00
abstract::On the basis of our previous observations indicating that a principle [possibly transforming growth factor-beta1 (TGFbeta1)] secreted by type 1 astrocytes may increase the release of LHRH in the GT1 cell line, it was deemed of interest to analyze whether TGFbeta1 might influence LHRH gene expression in addition to LHR...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940390
更新日期:1996-12-01 00:00:00
abstract::With a recently developed microdissection technique, four circumventricular organs were removed from the rat brain, and their contents of LHRH and TRH were measured. The subfornical organ, the organon vasculosum lamia terminalis, the subcommissural organ, the area postrema all contained significant quantities of both ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-2-311
更新日期:1976-02-01 00:00:00
abstract::The formation of ovarian follicles and subsequent development after birth are critical processes for female reproduction, and inappropriate coordination of these processes contributes to ovarian pathologies, such as premature ovarian failure and infertility. Identification and functional investigation of the factors i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0797
更新日期:2011-03-01 00:00:00
abstract::This study was designed to test the hypothesis that systemic immune challenge with endotoxin inhibits the reproductive axis centrally by suppressing GnRH pulsatile release into hypophyseal portal blood. Using alert, normally behaving, ovariectomized ewes, we sampled hypophyseal portal blood at 10-min intervals beginni...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5449
更新日期:1997-10-01 00:00:00
abstract::Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermatogenesis. It also prevents Leydig cells (LCs) from gonadotropin overstimulation of androgen production. In transgenic (Tg) mice carrying deletions...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2230
更新日期:2013-06-01 00:00:00
abstract::The existence of renin in a number of extrarenal tissues has been well documented, but the physiological role of extrarenal renin remains unknown. To study the physiological role of adrenal renin, we developed a serum-free culture system for adrenal capsular/zona glomrulosa explants. Explants showed good viability in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-486
更新日期:1989-07-01 00:00:00
abstract::The mechanism of the inhibitory effect of long term treatment with estradiol on dopamine synthesis in tuberoinfundibular dopaminergic (TIDA) neurons was studied by using hypothalamic slices from ovariectomized rats. Treatment with 2 mg estradiol valerate (EV) at a 3-week interval increased the weight of the anterior p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-692
更新日期:1987-08-01 00:00:00
abstract::Nuclear proteins extracted from purified nuclei with 0.4M KCl at pH 7.4 OR 8.5 are able to bind L-triiodothyronine (T3) giving rise to nuclear thyroid hormone binding protein-T3 (NTBP-T3) complexes. Binding is maximum in 3 h at 20 C. It is thermolabile even at 36 C, inhibited by p-hydroxymercuribenzoate and markedly e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-5-1201
更新日期:1975-05-01 00:00:00
abstract::Maternal nutrition can affect the susceptibility of the offspring to metabolic disease later in life, suggesting that this period is a window of opportunity for intervention to reduce the risk of metabolic disease. Resveratrol, a natural polyphenol, has a wide range of beneficial properties including anti-obesogenic, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa090
更新日期:2020-08-01 00:00:00
abstract::Binding of atrial and brain natriuretic peptides to guanylyl cyclase-A/natriuretic peptide receptor-A produces second messenger cGMP, which plays an important role in maintaining renal and cardiovascular homeostasis. Mice carrying a targeted disruption of the Npr1 gene coding for guanylyl cyclase-A/natriuretic peptide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0655
更新日期:2010-12-01 00:00:00
abstract::We have analyzed the factors responsible for the circadian variation in rat hepatic mRNA-S14. Regulation of this sequence, which is found in lipogenic tissues and encodes a protein (S14) believed to be associated with fatty acid synthesis, is an excellent model of the interaction of thyroid hormone and dietary factors...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1563
更新日期:1987-04-01 00:00:00
abstract::GH treatment increases protein deposition and the efficiency of dietary protein used for growth. To identify the mechanisms that regulate tissue protein synthesis in response to exogenous GH treatment, fully fed, growing swine were treated with GH for 7 d. Fasted and fed pigs were infused with [1-(13)C]leucine to dete...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220983
更新日期:2003-04-01 00:00:00
abstract::The present study assesses the relationship between G-proteins and protein kinase-C (PKC) in the gonadotrope. Cells were depleted of PKC with 1 microM phorbol 12-myristate 13-acetate for 12 h, followed by medium 199-BSA for 6 h before treatment with vehicle, pertussis toxin (PTX), cholera toxin (CTX), or (Bu)2cAMP (dB...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243300
更新日期:1993-12-01 00:00:00
abstract::Islet β-cell dysfunction that leads to impaired insulin secretion is a principal source of pathology of diabetes. In type 2 diabetes, this breakdown in β-cell health is associated with compromised islet-enriched transcription factor (TF) activity that disrupts gene expression programs essential for cell function and i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa213
更新日期:2021-02-01 00:00:00
abstract::Bromocriptine, a dopamine agonist, inhibited secretion of PRL and did not affect GH release from rat anterior pituitary cells in culture. The reversal of this inhibition of PRL release by butaclamol, a dopamine antagonist, was stereospecific; 10 nM d-butaclamol completely reversed the inhibition caused by 10 nM bromoc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-4-877
更新日期:1979-04-01 00:00:00
abstract::The ovulatory LH surge results in follicular inflammation with an increase in cytokines and PGs. Reactive oxygen species (ROS) are also produced during inflammatory processes. To study ROS generation during the ovulatory cascade, preovulatory follicles were dissected from immature female rats primed with PMSG. Follicl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.7961
更新日期:2001-02-01 00:00:00
abstract::The ileal proglucagon-derived peptides (PGDPs) play important roles in the regulation of gastric function and insulin secretion. We have previously demonstrated the existence of a proximal-distal enteroendocrine loop mediated by glucose-dependent insulinotropic peptide (GIP), through which intestinal PGDP secretion is...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641190
更新日期:1996-06-01 00:00:00
abstract::The TSH receptor (TSHR) is constitutively active and is further enhanced by TSH ligand binding or by stimulating TSHR antibodies (TSHR-Abs) as seen in Graves' disease. TSH is known to activate the thyroid epithelial cell via both Galphas-cAMP/protein kinase A/ERK and Galphaq-Akt/protein kinase C coupled signaling netw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0878
更新日期:2009-01-01 00:00:00
abstract::Immunoreactive neurophysin (Np) is demonstrated by the immunoperoxidase technique not only within neurones of the classical hypothalamo-neurohypophyseal system, but also in extrahypothalamic perikarya and fibers. Np-positive perikarya are found in the triangular nucleus of the septum. Np fibers join the stria terminal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-1-334
更新日期:1978-01-01 00:00:00