Effect of K+ deprivation and ouabain upon glucagon release.

Abstract:

:The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (8.3 mM) concentration of glucose, whereas ouabain enhanced glucagon secretion. In the presence of arginine, K+ deprivation induced limited and short lived oscillations in the rate of glucagon release at both low and high glucose levels. In the presence of arginine, ouabain induced first a short lived stimulation and, thereafter, a severe and sustained inhibition of glucagon release. The restoration of the normal K+ concentration or the removal of ouabain was followed by a marked and transient inhibition of glucagon release which was most prominent in the presence of arginine. In agreement with previous investigations, K+ deprivation or ouabain facilitated insulin release evoked by glucose and/or arginine. The results suggest that a primary alteration in K+ availability and/or transport affects in a dual manner the release of glucagon, depending on environmental factors such as the type of stimulus used to activate the secretory process.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Leclercq-Meyer V,Marchand J,Malaisse WJ

doi

10.1210/endo-113-1-178

subject

Has Abstract

pub_date

1983-07-01 00:00:00

pages

178-83

issue

1

eissn

0013-7227

issn

1945-7170

journal_volume

113

pub_type

杂志文章
  • Regulation of Sertoli cell differentiation by the testicular paracrine factor PModS: analysis of common signal transduction pathways.

    abstract::In the testis the mesenchymally derived peritubular cells produce a paracrine factor, PModS, that mediates mesenchymal-epithelial interactions and modulates Sertoli cell functions essential for the process of spermatogenesis. PModS has a more dramatic effect on Sertoli cell differentiated functions in vitro than any r...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.134.1.7903930

    authors: Norton JN,Vigne JL,Skinner MK

    更新日期:1994-01-01 00:00:00

  • The effect of carbonyl cyanide trifluoromethoxyphenylhydrazone and methylamine on the processing and secretion of the glycoprotein hormone chorionic gonadotropin by human choriocarcinoma cells.

    abstract::Carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP), a protonophore, and methylamine, a weak base, agents that dissipate hydrogen gradients across cellular membranes, were used to probe the coupling of hydrogen gradients to the processing and secretion of the glycoprotein hormone hCG by human choriocarcinoma cells...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-119-1-416

    authors: Peters BP,Krzesicki RF,Perini F,Ruddon RW

    更新日期:1986-07-01 00:00:00

  • Genome-Wide Estrogen Receptor Activity in Breast Cancer.

    abstract::The largest subtype of breast cancer is characterized by the expression and activity of the estrogen receptor alpha (ERalpha/ER). Although several effective therapies have significantly improved survival, the adaptability of cancer cells means that patients frequently stop responding or develop resistance to endocrine...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endocr/bqaa224

    authors: Farcas AM,Nagarajan S,Cosulich S,Carroll JS

    更新日期:2021-02-01 00:00:00

  • Carboxypeptidase H processing and secretion in rat clonal beta-cell lines.

    abstract::Carboxypeptidase H (CP-H, EC 3.4.17.10) removes C-terminal basic amino acids from insulin and many other peptide hormone intermediates in endocrine and neural tissues. CP-H was studied in two rat insulinoma-derived clonal beta-cell lines that were chosen in part because their profile of hormone production is restricte...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.131.3.1505450

    authors: Fiedorek FT Jr,Parkinson D

    更新日期:1992-09-01 00:00:00

  • Depot-specific modulation of rat intraabdominal adipose tissue lipid metabolism by pharmacological inhibition of 11beta-hydroxysteroid dehydrogenase type 1.

    abstract::The metabolic consequences of visceral obesity have been associated with amplification of glucocorticoid action by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in adipose tissue. This study aimed to assess in a rat model of diet-induced obesity the effects of pharmacological 11beta-HSD1 inhibition on the m...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2006-1199

    authors: Berthiaume M,Laplante M,Festuccia W,Gélinas Y,Poulin S,Lalonde J,Joanisse DR,Thieringer R,Deshaies Y

    更新日期:2007-05-01 00:00:00

  • An intramolecular disulfide bond between conserved extracellular cysteines in the gonadotropin-releasing hormone receptor is essential for binding and activation.

    abstract::In this study, site-directed mutagenesis and biochemical strategies have been used to establish whether disulfide bonding between extracellular Cys residues contributes to the structural integrity of the GnRH receptor (GnRH-R) and, if so, to delineate the nature of the bonding patterns involved. The majority of G prot...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.138.7.5233

    authors: Cook JV,Eidne KA

    更新日期:1997-07-01 00:00:00

  • Transforming growth factor-alpha is a potential mediator of estrogen action in the mouse uterus.

    abstract::To better understand the role of peptide growth factors in sex steroid hormone-mediated growth of the female reproductive tract, the effect of estrogen on the expression of transforming growth factor-alpha (TGF alpha) in mouse uterus was investigated. Our results show that estrogen induces the expression of TGF alpha ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.131.4.1396310

    authors: Nelson KG,Takahashi T,Lee DC,Luetteke NC,Bossert NL,Ross K,Eitzman BE,McLachlan JA

    更新日期:1992-10-01 00:00:00

  • Effect of dexamethasone on glucose tolerance and fat metabolism in a diet-induced obesity mouse model.

    abstract::Prolonged exposure to elevated glucocorticoid levels is known to produce insulin resistance (IR), a hallmark of diabetes mellitus. Although not fully elucidated, the underlying molecular mechanisms by which glucocorticoids induce IR may provide potential targets for pharmacological interventions. Here we characterized...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2007-1214

    authors: Gounarides JS,Korach-André M,Killary K,Argentieri G,Turner O,Laurent D

    更新日期:2008-02-01 00:00:00

  • Differential effects of insulin-like growth factor (IGF)-binding protein-3 and its proteolytic fragments on ligand binding, cell surface association, and IGF-I receptor signaling.

    abstract::Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3), the predominant IGF carrier protein in circulation, is posttranslationally modified in vivo by IGFBP-3 protease(s) into a number of fragments. Based on the ascertained and predicted recognition sites for known IGFBP-3 proteases, FLAG-epitope tagged intact I...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.141.11.7781

    authors: Devi GR,Yang DH,Rosenfeld RG,Oh Y

    更新日期:2000-11-01 00:00:00

  • The tyrosine phosphatase Shp-2 mediates intracellular signaling initiated by Ret mutants.

    abstract::The Src homology 2-containing tyrosine phosphatase, Shp-2, is a crucial enzyme that mediates intracellular signaling and is implicated in cell proliferation and differentiation. Here we investigated the involvement of the Shp-2 tyrosine phosphatase in determining the downstream signaling pathways initiated by the Ret ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2003-0620

    authors: D'Alessio A,Califano D,Incoronato M,Santelli G,Florio T,Schettini G,Carlomagno MS,Cerchia L,de Franciscis V

    更新日期:2003-10-01 00:00:00

  • Prolactin-releasing peptide as a novel stress mediator in the central nervous system.

    abstract::A1/A2 noradrenergic neurons in the medulla oblongata are well known to mediate stress signals in the central nervous system. Stress activates A1/A2 noradrenergic neurons, and then noradrenaline (NA) stimulates ACTH secretion through hypothalamic CRH. On the other hand, PRL-releasing peptide (PrRP) was recently isolate...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.142.5.8118

    authors: Maruyama M,Matsumoto H,Fujiwara K,Noguchi J,Kitada C,Fujino M,Inoue K

    更新日期:2001-05-01 00:00:00

  • Differential gonadotropin-releasing hormone (GnRH) and GnRH receptor messenger ribonucleic acid expression patterns in different tissues of the female rat across the estrous cycle.

    abstract::GnRH, the main regulator of reproduction, is produced in a variety of tissues outside of the hypothalamus, its main site of synthesis and release. We aimed to determine whether GnRH produced in the female rat pituitary and ovaries is involved in the processes leading to ovulation. We studied the expression patterns of...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2005-0240

    authors: Schirman-Hildesheim TD,Bar T,Ben-Aroya N,Koch Y

    更新日期:2005-08-01 00:00:00

  • Selective deletion of leptin receptors in gonadotropes reveals activin and GnRH-binding sites as leptin targets in support of fertility.

    abstract::The adipokine, leptin (LEP), is a hormonal gateway, signaling energy stores to appetite-regulatory neurons, permitting reproduction when stores are sufficient. Dual-labeling for LEP receptors (LEPRs) and gonadotropins or GH revealed a 2-fold increase in LEPR during proestrus, some of which was seen in LH gonadotropes....

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2014-1132

    authors: Akhter N,CarlLee T,Syed MM,Odle AK,Cozart MA,Haney AC,Allensworth-James ML,Beneš H,Childs GV

    更新日期:2014-10-01 00:00:00

  • Estradiol induces both qualitative and quantitative changes in the pattern of gonadotropin-releasing hormone secretion during the presurge period in the ewe.

    abstract::The follicular phase rise in circulating estradiol causes a suppression of GnRH pulse amplitude and an increase in pulse frequency before stimulation of the preovulatory GnRH surge in the ewe. Studies in which samples were collected at 10-min intervals were found unsuitable to determine whether GnRH secretion is exclu...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.136.4.7895670

    authors: Evans NP,Dahl GE,Mauger D,Karsch FJ

    更新日期:1995-04-01 00:00:00

  • Functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat.

    abstract::Frequent blood sampling via intraatrial cannula revealed daily rhythms of TSH and thyroid hormones in both male and female Wistar rats. Thermic ablation of the biological clock, i.e. the suprachiasmatic nucleus (SCN), eliminated the diurnal peak in circulating TSH and thyroid hormones. In addition, SCN lesions produce...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.141.10.7709

    authors: Kalsbeek A,Fliers E,Franke AN,Wortel J,Buijs RM

    更新日期:2000-10-01 00:00:00

  • Hormonal regulation of osteocalcin plasma production and clearance in sheep.

    abstract::The plasma osteocalcin (OC) concentration correlates with histological parameters of bone formation and has been used as an index of osteoblast activity. Although the rate of OC synthesis is likely to be a major determinant of the plasma OC level, the contribution of other processes, for example, OC plasma clearance, ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-124-6-2785

    authors: Fortune CL,Farrugia W,Tresham J,Scoggins BA,Wark JD

    更新日期:1989-06-01 00:00:00

  • Multiple effects of leucine on glucagon, insulin, and somatostatin secretion from the perfused rat pancreas.

    abstract::The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucos...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-116-3-1168

    authors: Leclercq-Meyer V,Marchand J,Woussen-Colle MC,Giroix MH,Malaisse WJ

    更新日期:1985-03-01 00:00:00

  • 1-(o-Chlorophenyl)-1 (p-chlorophenyl)2,2,2-trichloroethane induces functional progestin receptors in the rat hypothalamus and pituitary gland.

    abstract::A recent report suggests that estrogen induction of hypothalamic progestin receptors is not a prerequisite for the facilitation by progesterone of female sexual behavior in rats. Progesterone was found to facilitate sexual behavior despite no apparent induction of progestin receptors by 1-(o-chlorophenyl) 1-(p-chlorop...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-115-6-2052

    authors: Brown TJ,Blaustein JD

    更新日期:1984-12-01 00:00:00

  • Anabolic effects of 1,25-dihydroxyvitamin D3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells.

    abstract::Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.138.7.5275

    authors: Wang DS,Miura M,Demura H,Sato K

    更新日期:1997-07-01 00:00:00

  • Localization of olfactory cyclic nucleotide-gated channels in rat gonadotropin-releasing hormone neurons.

    abstract::The GT1 GnRH cell lines express all three subunits of the cyclic nucleotide-gated (CNG) channels (CNG2, 4.3 and 5) expressed in olfactory neurons. We investigated using in situ hybridization and double immunofluorescence whether endogenous GnRH neurons in therat also express CNG channel subunits. Sections from male an...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.143.6.8951

    authors: El-Majdoubi M,Weiner RI

    更新日期:2002-06-01 00:00:00

  • Connective tissue growth factor (IGFBP-rP2) expression and regulation in cultured bovine endothelial cells.

    abstract::Media from large vessel endothelial cells (pulmonary artery, aorta) contained intact connective tissue growth factor (CTGF) and a dominant 19-kDa band. N-terminal analysis of the 19-kDa band showed sequence corresponding to CTGF amino acid 181-190, suggesting that the 19-kDa band represented a proteolytic fragment of ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.140.4.6633

    authors: Boes M,Dake BL,Booth BA,Erondu NE,Oh Y,Hwa V,Rosenfeld R,Bar RS

    更新日期:1999-04-01 00:00:00

  • Human uridine diphosphate-glucuronosyltransferase UGT2B7 conjugates mineralocorticoid and glucocorticoid metabolites.

    abstract::Mineralocorticoid and glucocorticoid hormones are metabolized as glucuronide conjugates. Using labeled [(14)C]uridine diphosphate glucuronic acid and microsomal preparations from human embryonic kidney 293 cells stably expressing the different human and monkey uridine diphosphate glucuronosyltransferase (UGT)2B enzyme...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-0052

    authors: Girard C,Barbier O,Veilleux G,El-Alfy M,Bélanger A

    更新日期:2003-06-01 00:00:00

  • Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: biochemical and in situ detection of deoxyribonucleic acid fragmentation in granulosa cells.

    abstract::The majority of ovarian follicles undergo atresia through a mechanism involving apoptotic cell death. Although GnRH and its agonists have been shown to suppress ovarian growth and differentiation in hypophysectomized rats, studies on the induction of follicle atresia by GnRH are contradictory. In the present study, th...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.134.1.8275940

    authors: Billig H,Furuta I,Hsueh AJ

    更新日期:1994-01-01 00:00:00

  • Rab8B GTPase and junction dynamics in the testis.

    abstract::Throughout spermatogenesis, germ cells migrate from the basal to the adluminal compartment while remaining attached to Sertoli cells via actin-based adherens and intermediate filament-based anchoring junctions. However, the events that trigger deadhesion and adhesion remain largely unknown. As part of our continued ef...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-220893

    authors: Lau AS,Mruk DD

    更新日期:2003-04-01 00:00:00

  • Unexpected virilization in male mice lacking steroid 5 alpha-reductase enzymes.

    abstract::Mice lacking steroid 5 alpha-reductase 1 and 2 were produced by gene targeting and breeding. Male mice without 5 alpha-reductase 2 or without both enzymes had fully formed internal and external genitalia and were fertile, but had smaller prostates and seminal vesicles than controls. T accumulated to high levels in the...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.142.11.8510

    authors: Mahendroo MS,Cala KM,Hess DL,Russell DW

    更新日期:2001-11-01 00:00:00

  • Developmental exposure to Bisphenol a impairs the uterine response to ovarian steroids in the adult.

    abstract::Morphoregulator genes like members of the Hox gene family regulate uterine development and are associated with endocrine-related processes such as endometrial proliferation and differentiation in the adult uterus. Exposure to neonatal endocrine disruptors could affect signaling events governed by Hox genes, altering t...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2008-0651

    authors: Varayoud J,Ramos JG,Bosquiazzo VL,Muñoz-de-Toro M,Luque EH

    更新日期:2008-11-01 00:00:00

  • Calmodulin dependence of somatostatin release stimulated by growth hormone-releasing factor.

    abstract::Experiments were performed in vitro to examine the possible role of calcium and calmodulin in GRF-induced somatostatin (SRIF) release from the median eminence. Adult male rats were used as tissue donors. The median eminences were first prestimulated in 0.4 ml Krebs Ringer bicarbonate glucose buffer (pH 7.4) containing...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-123-1-305

    authors: Aguila MC,McCann SM

    更新日期:1988-07-01 00:00:00

  • Metformin inhibits androgen-induced IGF-IR up-regulation in prostate cancer cells by disrupting membrane-initiated androgen signaling.

    abstract::We have previously demonstrated that, in prostate cancer cells, androgens up-regulate IGF-I receptor (IGF-IR) by inducing cAMP-response element-binding protein (CREB) activation and CREB-dependent IGF-IR gene transcription through androgen receptor (AR)-dependent membrane-initiated effects. This IGF-IR up-regulation i...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2013-1925

    authors: Malaguarnera R,Sacco A,Morcavallo A,Squatrito S,Migliaccio A,Morrione A,Maggiolini M,Belfiore A

    更新日期:2014-04-01 00:00:00

  • Autoregulation of pituitary growth hormone messenger ribonucleic acid levels in rats bearing transplantable mammosomatotrophic pituitary tumors.

    abstract::Female Wistar-Furth rats were implanted sc with GH3 rat pituitary tumor cells. Tumors were palpable by 4 weeks, and animals were killed periodically from 5-9 weeks. Tumor-bearing rats (n = 10) were heavier than their respective controls, reaching a weight of 372 +/- 3 by 9 weeks vs. 195 +/- 5 g in controls (mean +/- S...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-118-3-915

    authors: Yamashita S,Slanina S,Kado H,Melmed S

    更新日期:1986-03-01 00:00:00

  • Prolonged activation of ERK2 by epidermal growth factor and other growth factors requires a functional insulin-like growth factor 1 receptor.

    abstract::We have investigated the activation of ERK2, a serine/threonine kinase necessary for transmission of mitogenic signals, in cells derived from mouse embryos homozygous for a null mutation of the insulin-like growth factor (IGF)-1R gene (R- cells) and from wild-type littermates (W cells), respectively. Stimulation of qu...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.140.7.6766

    authors: Swantek JL,Baserga R

    更新日期:1999-07-01 00:00:00