Abstract:
:Experiments were conducted to investigate the actions of glucocorticoids and insulin on the induction of a specific mRNA (p33-mRNA) expressed in rat H4 hepatoma cells. Previous studies have found a 10-fold increase in this mRNA following 60 min of insulin addition. In the present study, dexamethasone (Dex) induced the cellular concentration of p33-mRNA 10- to 15-fold. This effect was time and dose dependent. The effect of Dex could be accounted for by a 10- to 15-fold increase in p33-mRNA transcription. However, insulin administration resulted in only a 3-fold increase in the transcription of p33-mRNA. The insulin induction of transcription was time and dose dependent and was blocked by the addition of alpha-amanitin. There was no increase in the transcription of a control gene, beta-tubulin, by either insulin or Dex. Neither insulin nor Dex altered the stability of p33-mRNA. Since the cellular concentration of p33-mRNA was induced to a greater extent than was transcription, insulin must be regulating at least one other step in the sequence between RNA synthesis and RNA stability.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Messina JLdoi
10.1210/endo-124-2-754subject
Has Abstractpub_date
1989-02-01 00:00:00pages
754-61issue
2eissn
0013-7227issn
1945-7170journal_volume
124pub_type
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