Abstract:
:The present study was designed to evaluate the relative contribution of endogenous excitatory amino acids to the control of the estradiol-induced LH surge using specific blockers for N-methyl-D-aspartic acid (NMDA) and non-NMDA receptor types. Adult female rats ovariectomized for 2-3 weeks were implanted with third ventricular cannulae one week before the experiments. Silastic capsules (3 cm active surface) containing estradiol benzoate (250 micrograms/ml dissolved in sesame oil) were implanted subcutaneously two days prior to bleeding. Blood samples were collected at hourly intervals (from 1300 to 2100 h) through indwelling atrial cannulae implanted the day before the bleeding. (+) 2-amino-7-phosphoheptanoic acid (AP-7), a NMDA receptor antagonist, and 6,7-dinitroquinoxaline-2,3-dione (DNQX), a non-NMDA receptor antagonist, were administered (10 and 20 nmole dissolved in 10 microliters 0.9% sodium chloride, respectively) at 1300 and 1400 h into the third ventricle. LH, FSH and PRL levels were assayed by RIA in plasma samples. AP-7 and DNQX administration completely blocked the estradiol-induced LH surge, whereas PRL and FSH secretion was not affected by the treatments. These results indicate that endogenous EAA play an important role in controlling LH secretion. Furthermore, the study reveals that both EAA receptor types; i.e. NMDA and non-NMDA, appear to be necessary for the physiological mechanism(s) triggering the estradiol-induced LH surge.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
López FJ,Donoso AO,Negro-Vilar Adoi
10.1210/endo-126-3-1771subject
Has Abstractpub_date
1990-03-01 00:00:00pages
1771-3issue
3eissn
0013-7227issn
1945-7170journal_volume
126pub_type
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