Altered endocrine and autocrine metabolism of vitamin D in a mouse model of gastrointestinal inflammation.

Abstract:

:The active form of vitamin D, 1,25-dihydroxyvitamin D3, [1,25(OH)2D3] has potent actions on innate and adaptive immunity. Although endocrine synthesis of 1,25(OH)2D3 takes place in the kidney, the enzyme that catalyzes this, 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27b1 in humans, Cyp27b1 in mice), is expressed at many extra-renal sites including the colon. We have shown previously that colonic expression of CYP27b1 may act to protect against the onset of colitis. To investigate this further, we firstly characterized changes in Cyp27b1 expression in a mouse model of colitis. Mice treated with dextran sodium sulfate (DSS) showed weight loss, histological evidence of colitis, and increased expression of inflammatory cytokines. This was associated with decreased renal expression of Cyp27b1 (5-fold, P=0.013) and lower serum 1,25(OH)2D3 (51.8+/-5.9 pg/nl vs. 65.1+/-1.6 in controls, P<0.001). However, expression of CYP27b1 was increased in the proximal colon of DSS mice (4-fold compared with controls, P<0.001). Further studies were carried out using Cyp27b1 null (-/-) mice. Compared with+/-controls the Cyp27b1-/-mice showed increased weight loss (4.9% vs. 22.8%, P<0.001) and colitis. This was associated with raised IL-1 in the distal colon and IL-17 in the proximal and distal colon. Conversely, DSS-treated Cyp27b1-/-mice exhibited lower IL-10 in the proximal colon and toll-like receptors 2 and 4 in the distal colon. These data indicate that both local and endocrine synthesis of 1,25(OH)2D3 affect colitis in DSS-treated mice. Lack of Cyp27b1 exacerbates disease in this model, suggesting that similar effects may occur with vitamin D deficiency.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Liu N,Nguyen L,Chun RF,Lagishetty V,Ren S,Wu S,Hollis B,DeLuca HF,Adams JS,Hewison M

doi

10.1210/en.2008-0060

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

4799-808

issue

10

eissn

0013-7227

issn

1945-7170

pii

en.2008-0060

journal_volume

149

pub_type

杂志文章
  • The regulation of insulin-like growth factor-binding protein 1 messenger ribonucleic acid in cultured rat hepatocytes: the roles of glucagon and growth hormone.

    abstract::In previous studies it was shown that bovine GH (bGH) suppressed and glucagon stimulated the level of 24- and 30- to 34-kilodalton insulin-like growth factor-binding proteins (IGFBPs) in the media of cultured rat hepatocytes. In the present study we have evaluated the regulation of IGFBP-1 gene expression in primary r...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.135.5.7525250

    authors: Kachra Z,Yang CR,Murphy LJ,Posner BI

    更新日期:1994-11-01 00:00:00

  • IL-1alpha and TNFalpha down-regulate CRH receptor-2 mRNA expression in the mouse heart.

    abstract::Two receptors (CRH receptor type 1 and CRH receptor type 2) have been identified for the stress-induced neuropeptide, CRH and related peptides, urocortin, and urocortin II. We previously found marked down-regulation of cardiac CRH receptor type 2 expression following administration of bacterial endotoxin, lipopolysacc...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.142.8.8342

    authors: Coste SC,Heldwein KA,Stevens SL,Tobar-Dupres E,Stenzel-Poore MP

    更新日期:2001-08-01 00:00:00

  • Monocarboxylate transporter 10 functions as a thyroid hormone transporter in chondrocytes.

    abstract::Thyroid hormone is essential for normal proliferation and differentiation of chondrocytes. Thus, untreated congenital hypothyroidism is marked by severe short stature. The monocarboxylate transporter 8 (MCT8) is a highly specific transporter for thyroid hormone. The hallmarks of Allan-Herndon-Dudley syndrome, caused b...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2011-1713

    authors: Abe S,Namba N,Abe M,Fujiwara M,Aikawa T,Kogo M,Ozono K

    更新日期:2012-08-01 00:00:00

  • Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor.

    abstract::Primary functional bovine adrenal cortical cell cultures have been developed to study the factors controlling adrenal cell growth. Cells were prepared by the collagenase technique and maintained in F-12 medium containing fetal calf serum and horse serum. Cells contained abundant lipid as demonstrated by staining with ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-100-4-1080

    authors: Gospodarowicz D,Ill CR,Hornsby PJ,Gill GN

    更新日期:1977-04-01 00:00:00

  • Inhibition of adenylyl cyclase by caveolin peptides.

    abstract::Caveolae and their principal component caveolin have been implicated in playing a major role in G protein-mediated transmembrane signaling. We examined whether caveolin interacts with adenylyl cyclase, an effector of G protein signaling, using a 20-mer peptide derived from the N-terminus scaffolding domain of caveolin...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.139.4.5957

    authors: Toya Y,Schwencke C,Couet J,Lisanti MP,Ishikawa Y

    更新日期:1998-04-01 00:00:00

  • Galanin-like peptide stimulates food intake via activation of neuropeptide Y neurons in the hypothalamic dorsomedial nucleus of the rat.

    abstract::Galanin-like peptide (GALP), a 29-amino-acid neuropeptide, is located in the hypothalamic arcuate nucleus (ARC), binds to galanin receptor subtype 2, and induces food intake upon intracerebroventricular (icv) injection in rats. However, neural mechanisms underlying its orexigenic action remain unclear. We aimed to ide...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2005-0907

    authors: Kuramochi M,Onaka T,Kohno D,Kato S,Yada T

    更新日期:2006-04-01 00:00:00

  • The effect of somatostatin on the release of thyrotropin and its subunits from bovine anterior pituitary cells in vitro.

    abstract::The effects of somatostatin (SRIF) on the production and release of TSH and its subunits have been investigated in bovine anterior pituitary monolayer cultures. SRIF caused a dose-dependent inhibition of TSH and subunit release by TRH, with a half-maximal effective dose of 3 X 10(-8) M. This effect was time dependent ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-112-6-1937

    authors: Ridgway EC,Klibanski A,Martorana MA,Milbury P,Kieffer JD,Chin WW

    更新日期:1983-06-01 00:00:00

  • Adrenal medullary responses to repeated hemorrhage in the anesthetized dog.

    abstract::Adrenal secretory rates of catecholamines in response to a repeated stimulus have not been described. We have used repeated small hemorrhages spaced 24 h apart in pentobarbital anesthetized dogs prepared with a chronic adrenal venous catheter to study this question. Our results confirm earlier reports that an initial ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-111-6-1917

    authors: Lilly MP,Engeland WC,Gann DS

    更新日期:1982-12-01 00:00:00

  • A targeted thyroid hormone receptor alpha gene dominant-negative mutation (P398H) selectively impairs gene expression in differentiated embryonic stem cells.

    abstract::Thyroid hormone and retinoic acid (RA) are essential for normal neural development in vivo, yet all in vitro differentiation strategies of embryonic stem (ES) cells use only RA. We developed a novel differentiation strategy of mouse ES cells using T(3). A dominant-negative knock-in point mutation (P398H) was introduce...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.143.7.8906

    authors: Liu YY,Tachiki KH,Brent GA

    更新日期:2002-07-01 00:00:00

  • Parathyroid hormone degradation by chymotrypsin-like endopeptidase in the opossum kidney cell.

    abstract::Cathepsin-D has been previously reported to cleave intact PTH into PTH-(1-34) and -(35-84) in membranous fractions of rat and bovine kidney. Whether PTH degradation occurs by intact kidney cells, however, has not been examined in detail. We have, therefore, examined this possibility using an opossum kidney (OK) cell l...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-123-6-2812

    authors: Yamaguchi T,Fukase M,Nishikawa M,Fujimi T,Fujita T

    更新日期:1988-12-01 00:00:00

  • Dysregulation of corticosterone secretion in streptozotocin-diabetic rats: modulatory role of the adrenocortical nitrergic system.

    abstract::An increased activity of the hypothalamo-pituitary-adrenal axis resulting in exaggerated glucocorticoid secretion has been repeatedly described in patients with diabetes mellitus and in animal models of this disease. However, it has been pointed out that experimental diabetes is accompanied by a decreased glucocortico...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2009-0592

    authors: Repetto EM,Sanchez R,Cipelli J,Astort F,Calejman CM,Piroli GG,Arias P,Cymeryng CB

    更新日期:2010-01-01 00:00:00

  • Bovine thyrotropin has a specific bioactivity 5- to 10-fold that of previous estimates for highly purified hormone.

    abstract::Bovine TSH (bTSH) was subjected to reverse phase HPLC using a Synchropak RP-P C18 column. Elution was with a linear gradient of 0-50% 1-propranol in 0.1% trifluoroacetic acid. Individual fractions were assayed for TSH bioactivity by the cultured thyroid cell cAMP response assay. Optimum separation of biologically acti...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-116-4-1379

    authors: Rapoport B,Seto P

    更新日期:1985-04-01 00:00:00

  • Estrogen-induced calbindin-D 9k gene expression in the rat uterus during the estrous cycle: late antagonistic effect of progesterone.

    abstract::Progesterone modulates estrogen-stimulated responses in the uterus. Calbindin-D 9k (CaBP9k), a 17 beta-estradiol-responsive gene expressed in the uterus, was used as a marker to examine the interactions between endogenous progesterone and estradiol in the rat. The variations in uterine CaBP9k messenger RNAs (mRNAs) du...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.132.2.8425470

    authors: L'Horset F,Blin C,Brehier A,Thomasset M,Perret C

    更新日期:1993-02-01 00:00:00

  • Pyridine nucleotides in pancreatic islets during inhibition of insulin release by exogenous insulin.

    abstract::In vitro addition of rat insulin (200, 400 or 800 muU/ml) to collagenase-isolated pancreatic islets of adult rats diminished glucose (3 mg/ml)-induced insulin release which was correlated with a decrease of the ratio of total NADPH/NADP and inhibition of glucose oxidation via the pentose phosphate shunt (PPS). NADH an...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-99-6-1469

    authors: Ammon HP,Verspohl E

    更新日期:1976-12-01 00:00:00

  • The mineralocorticoid antagonist activity of an 11 beta,18-oxidopregnane.

    abstract::Removal of the 18-hydroxy group of the hemiacetal form of aldosterone transforms its activity from that of pure agonist to predominant antagonist. The 18-deoxy derivative possesses one third of the binding affinity of aldosterone for the cytoplasmic mineralocorticoid receptor of rat kidney and exhibits an approximate ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-104-5-1352

    authors: Ulick S,Marver D,Adam WR,Funder JW

    更新日期:1979-05-01 00:00:00

  • Phosphorylation of mitochondrial proteins in isolated porcine ovarian follicles after treatment with luteinizing hormone.

    abstract::LH treatment of isolated medium (3- to 7-mm) follicles from porcine ovaries stimulated pregnenolone synthesis by mitochondria isolated from these tissues. This LH stimulation of pregnenolone synthesis was observed regardless of whether exogenous cholesterol was added to the mitochondrial assay system, suggesting that ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-114-2-588

    authors: Neymark MA,Bieszczad RR,Dimino MJ

    更新日期:1984-02-01 00:00:00

  • Characterization and expression of steroidogenic acute regulatory protein and MLN64 cDNAs in trout.

    abstract::Complementary DNA-encoding proteins with high homology to steroidogenic acute regulatory proteins (StAR) of mammals were cloned from rainbow trout head kidney and a mixture of several brook trout tissues. A cDNA encoding an MLN64 homolog was also cloned from brook trout. The C-terminal domains of rainbow trout StAR an...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.143.6.8672

    authors: Kusakabe M,Todo T,McQuillan HJ,Goetz FW,Young G

    更新日期:2002-06-01 00:00:00

  • Diurnal variation in hepatic expression of the rat S14 gene is synchronized by the photoperiod.

    abstract::We have analyzed the factors responsible for the circadian variation in rat hepatic mRNA-S14. Regulation of this sequence, which is found in lipogenic tissues and encodes a protein (S14) believed to be associated with fatty acid synthesis, is an excellent model of the interaction of thyroid hormone and dietary factors...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-120-4-1563

    authors: Kinlaw WB,Fish LH,Schwartz HL,Oppenheimer JH

    更新日期:1987-04-01 00:00:00

  • Regulation of insulin-like growth factor I receptor promoter activity by wild-type and mutant versions of the WT1 tumor suppressor.

    abstract::The insulin-like growth factor I (IGF-I) receptor is a transmembrane tyrosine kinase that mediates the growth-promoting effects of IGF-I and IGF-II. Changes in IGF-I receptor messenger RNA levels are reflected in cell surface receptor number, and modulation of IGF-I receptor levels affects tumorigenicity in numerous c...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.140.10.7065

    authors: Tajinda K,Carroll J,Roberts CT Jr

    更新日期:1999-10-01 00:00:00

  • Sterol regulatory element binding protein-1a transactivates 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene promoter.

    abstract::6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key modulator of glycolysis-gluconeogenesis. To gain insight into the molecular mechanism behind hormonal and nutritional regulation of PFKFB expression, we have cloned and characterized...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2005-1506

    authors: Metón I,Egea M,Anemaet IG,Fernández F,Baanante IV

    更新日期:2006-07-01 00:00:00

  • Endoplasmic reticulum stress-induced activation of activating transcription factor 6 decreases cAMP-stimulated hepatic gluconeogenesis via inhibition of CREB.

    abstract::The expression of genes encoding key hepatic gluconeogenic enzymes, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), is regulated at the transcriptional level by a network of transcription factors and cofactors, including cAMP response element-binding protein (CREB). It has been ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2009-0641

    authors: Seo HY,Kim MK,Min AK,Kim HS,Ryu SY,Kim NK,Lee KM,Kim HJ,Choi HS,Lee KU,Park KG,Lee IK

    更新日期:2010-02-01 00:00:00

  • Low degree of overlap between kisspeptin, neurokinin B, and dynorphin immunoreactivities in the infundibular nucleus of young male human subjects challenges the KNDy neuron concept.

    abstract::Previous immunohistochemical and in situ hybridization studies of sheep, goats, and rodents indicated that kisspeptin (KP), neurokinin B (NKB), and dynorphin A (DYN) are extensively colocalized in the hypothalamic arcuate nucleus, thus providing a basis for the KP/NKB/DYN (KNDy) neuron concept; in both sexes, KNDy neu...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2012-1545

    authors: Hrabovszky E,Sipos MT,Molnár CS,Ciofi P,Borsay BÁ,Gergely P,Herczeg L,Bloom SR,Ghatei MA,Dhillo WS,Liposits Z

    更新日期:2012-10-01 00:00:00

  • S14: insights from knockout mice.

    abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...

    journal_title:Endocrinology

    pub_type: 杂志文章,评审

    doi:10.1210/en.2006-0473

    authors: LaFave LT,Augustin LB,Mariash CN

    更新日期:2006-09-01 00:00:00

  • Stimulation of renal prostaglandin synthesis by the pressor activity of vasopressin.

    abstract::Renal prostaglandin E2 (PGE2) synthesis is enhanced by exogenous vasopressin (AVP). To determine whether the pressor or the antidiuretic activities of AVP are related to its effect on PGE2 synthesis, AVP and the nonpressor analog, 1-deamino-8-D-arginine vasopressin (dD'AVP), were administered to the isolated perfused ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-108-2-495

    authors: Zipser RD,Myers SI,Needleman P

    更新日期:1981-02-01 00:00:00

  • Unbound progesterone receptors are in equilibrium between the nucleus and cytoplasm in cells of the rat uterus.

    abstract::The classical model for the mechanism of action of steroids holds that unbound receptors for steroids reside exclusively in the cytoplasmic compartment and that they undergo translocation to the nucleus when bound to steroids in a process which is temperature sensitive. We have in the past proposed that unbound recept...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-108-4-1533

    authors: Sheridan PJ,Buchanan JM,Anselmo VC,Martin PM

    更新日期:1981-04-01 00:00:00

  • Regulation of matrix metalloproteinases (MMP-2, -3, -9, and -13) by interleukin-1 and interleukin-6 in mouse calvaria: association of MMP induction with bone resorption.

    abstract::Interleukin-1 (IL-1) greatly induces osteoclast formation and stimulates bone resorption of mouse calvaria in culture. In the presence of soluble IL-6 receptor (sIL-6R), IL-6 similarly induces osteoclast formation, but the potency of IL-6 in inducing bone resorption in organ culture is weaker than that of IL-1. To stu...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.139.3.5818

    authors: Kusano K,Miyaura C,Inada M,Tamura T,Ito A,Nagase H,Kamoi K,Suda T

    更新日期:1998-03-01 00:00:00

  • Stanozolol and danazol, unlike natural androgens, interact with the low affinity glucocorticoid-binding sites from male rat liver microsomes.

    abstract::Some 17 alpha-alkylated androgens used as anabolic agents, such as stanozolol (ST) and danazol (DA), have specific effects on the liver that are not exerted by testosterone. This gives rise to the possibility that a steroid-binding protein, other than the androgen receptor, could modulate the intracellular actions of ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.134.3.8119180

    authors: Fernández L,Chirino R,Boada LD,Navarro D,Cabrera N,del Rio I,Díaz-Chico BN

    更新日期:1994-03-01 00:00:00

  • Calcitonin inhibits thyrotropin-releasing hormone-induced increases in cytosolic Ca2+ in isolated rat anterior pituitary cells.

    abstract::Calcitonin (CT) and related peptides, such as CT gene-related peptide and salmon CT (sCT)-like peptide, are present in the rat nervous system and the pituitary gland, and sCT markedly inhibits basal and TRH-stimulated PRL release from anterior pituitary (AP) cells. Because TRH-induced PRL release is known to involve i...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-127-2-613

    authors: Shah GV,Kennedy D,Dockter ME,Crowley WR

    更新日期:1990-08-01 00:00:00

  • Changes in insulin, somatostatin, and glucagon secretion during the development of obesity in ventromedial hypothalamic-lesioned rats.

    abstract::Changes in insulin, somatostatin, and glucagon secretion during the development of obesity in rats after ventromedial hypothalamic (VMH) lesions were evaluated by measuring fasting hormone levels and their secretion from the isolated perfused pancreas. Fasting peripheral insulin levels were not altered 1 week after th...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-114-2-457

    authors: Seino Y,Seino S,Takemura J,Tsuda K,Nishi S,Ishida H,Seno M,Usami M,Ikeda M,Imura H

    更新日期:1984-02-01 00:00:00

  • Progesterone-induced secretion of dipeptidyl peptidase-IV (cluster differentiation antigen-26) by the uterine endometrium of the ewe and cow that costimulates lymphocyte proliferation.

    abstract::Dipeptidyl peptidase-IV (DPPIV) is a serine proteinase widely distributed in mammalian tissues, including lymphocytes, where it is identical to the T-cell activation antigen, cluster differentiation antigen-26. In the present study, DPPIV enzymatic activity was found in uterine secretions of unilaterally pregnant ewes...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.136.2.7835310

    authors: Liu WJ,Hansen PJ

    更新日期:1995-02-01 00:00:00