Abstract:
:The active form of vitamin D, 1,25-dihydroxyvitamin D3, [1,25(OH)2D3] has potent actions on innate and adaptive immunity. Although endocrine synthesis of 1,25(OH)2D3 takes place in the kidney, the enzyme that catalyzes this, 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27b1 in humans, Cyp27b1 in mice), is expressed at many extra-renal sites including the colon. We have shown previously that colonic expression of CYP27b1 may act to protect against the onset of colitis. To investigate this further, we firstly characterized changes in Cyp27b1 expression in a mouse model of colitis. Mice treated with dextran sodium sulfate (DSS) showed weight loss, histological evidence of colitis, and increased expression of inflammatory cytokines. This was associated with decreased renal expression of Cyp27b1 (5-fold, P=0.013) and lower serum 1,25(OH)2D3 (51.8+/-5.9 pg/nl vs. 65.1+/-1.6 in controls, P<0.001). However, expression of CYP27b1 was increased in the proximal colon of DSS mice (4-fold compared with controls, P<0.001). Further studies were carried out using Cyp27b1 null (-/-) mice. Compared with+/-controls the Cyp27b1-/-mice showed increased weight loss (4.9% vs. 22.8%, P<0.001) and colitis. This was associated with raised IL-1 in the distal colon and IL-17 in the proximal and distal colon. Conversely, DSS-treated Cyp27b1-/-mice exhibited lower IL-10 in the proximal colon and toll-like receptors 2 and 4 in the distal colon. These data indicate that both local and endocrine synthesis of 1,25(OH)2D3 affect colitis in DSS-treated mice. Lack of Cyp27b1 exacerbates disease in this model, suggesting that similar effects may occur with vitamin D deficiency.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Liu N,Nguyen L,Chun RF,Lagishetty V,Ren S,Wu S,Hollis B,DeLuca HF,Adams JS,Hewison Mdoi
10.1210/en.2008-0060subject
Has Abstractpub_date
2008-10-01 00:00:00pages
4799-808issue
10eissn
0013-7227issn
1945-7170pii
en.2008-0060journal_volume
149pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::In previous studies it was shown that bovine GH (bGH) suppressed and glucagon stimulated the level of 24- and 30- to 34-kilodalton insulin-like growth factor-binding proteins (IGFBPs) in the media of cultured rat hepatocytes. In the present study we have evaluated the regulation of IGFBP-1 gene expression in primary r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7525250
更新日期:1994-11-01 00:00:00
abstract::Two receptors (CRH receptor type 1 and CRH receptor type 2) have been identified for the stress-induced neuropeptide, CRH and related peptides, urocortin, and urocortin II. We previously found marked down-regulation of cardiac CRH receptor type 2 expression following administration of bacterial endotoxin, lipopolysacc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8342
更新日期:2001-08-01 00:00:00
abstract::Thyroid hormone is essential for normal proliferation and differentiation of chondrocytes. Thus, untreated congenital hypothyroidism is marked by severe short stature. The monocarboxylate transporter 8 (MCT8) is a highly specific transporter for thyroid hormone. The hallmarks of Allan-Herndon-Dudley syndrome, caused b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1713
更新日期:2012-08-01 00:00:00
abstract::Primary functional bovine adrenal cortical cell cultures have been developed to study the factors controlling adrenal cell growth. Cells were prepared by the collagenase technique and maintained in F-12 medium containing fetal calf serum and horse serum. Cells contained abundant lipid as demonstrated by staining with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-4-1080
更新日期:1977-04-01 00:00:00
abstract::Caveolae and their principal component caveolin have been implicated in playing a major role in G protein-mediated transmembrane signaling. We examined whether caveolin interacts with adenylyl cyclase, an effector of G protein signaling, using a 20-mer peptide derived from the N-terminus scaffolding domain of caveolin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5957
更新日期:1998-04-01 00:00:00
abstract::Galanin-like peptide (GALP), a 29-amino-acid neuropeptide, is located in the hypothalamic arcuate nucleus (ARC), binds to galanin receptor subtype 2, and induces food intake upon intracerebroventricular (icv) injection in rats. However, neural mechanisms underlying its orexigenic action remain unclear. We aimed to ide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0907
更新日期:2006-04-01 00:00:00
abstract::The effects of somatostatin (SRIF) on the production and release of TSH and its subunits have been investigated in bovine anterior pituitary monolayer cultures. SRIF caused a dose-dependent inhibition of TSH and subunit release by TRH, with a half-maximal effective dose of 3 X 10(-8) M. This effect was time dependent ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-1937
更新日期:1983-06-01 00:00:00
abstract::Adrenal secretory rates of catecholamines in response to a repeated stimulus have not been described. We have used repeated small hemorrhages spaced 24 h apart in pentobarbital anesthetized dogs prepared with a chronic adrenal venous catheter to study this question. Our results confirm earlier reports that an initial ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1917
更新日期:1982-12-01 00:00:00
abstract::Thyroid hormone and retinoic acid (RA) are essential for normal neural development in vivo, yet all in vitro differentiation strategies of embryonic stem (ES) cells use only RA. We developed a novel differentiation strategy of mouse ES cells using T(3). A dominant-negative knock-in point mutation (P398H) was introduce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.7.8906
更新日期:2002-07-01 00:00:00
abstract::Cathepsin-D has been previously reported to cleave intact PTH into PTH-(1-34) and -(35-84) in membranous fractions of rat and bovine kidney. Whether PTH degradation occurs by intact kidney cells, however, has not been examined in detail. We have, therefore, examined this possibility using an opossum kidney (OK) cell l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2812
更新日期:1988-12-01 00:00:00
abstract::An increased activity of the hypothalamo-pituitary-adrenal axis resulting in exaggerated glucocorticoid secretion has been repeatedly described in patients with diabetes mellitus and in animal models of this disease. However, it has been pointed out that experimental diabetes is accompanied by a decreased glucocortico...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0592
更新日期:2010-01-01 00:00:00
abstract::Bovine TSH (bTSH) was subjected to reverse phase HPLC using a Synchropak RP-P C18 column. Elution was with a linear gradient of 0-50% 1-propranol in 0.1% trifluoroacetic acid. Individual fractions were assayed for TSH bioactivity by the cultured thyroid cell cAMP response assay. Optimum separation of biologically acti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1379
更新日期:1985-04-01 00:00:00
abstract::Progesterone modulates estrogen-stimulated responses in the uterus. Calbindin-D 9k (CaBP9k), a 17 beta-estradiol-responsive gene expressed in the uterus, was used as a marker to examine the interactions between endogenous progesterone and estradiol in the rat. The variations in uterine CaBP9k messenger RNAs (mRNAs) du...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425470
更新日期:1993-02-01 00:00:00
abstract::In vitro addition of rat insulin (200, 400 or 800 muU/ml) to collagenase-isolated pancreatic islets of adult rats diminished glucose (3 mg/ml)-induced insulin release which was correlated with a decrease of the ratio of total NADPH/NADP and inhibition of glucose oxidation via the pentose phosphate shunt (PPS). NADH an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1469
更新日期:1976-12-01 00:00:00
abstract::Removal of the 18-hydroxy group of the hemiacetal form of aldosterone transforms its activity from that of pure agonist to predominant antagonist. The 18-deoxy derivative possesses one third of the binding affinity of aldosterone for the cytoplasmic mineralocorticoid receptor of rat kidney and exhibits an approximate ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-5-1352
更新日期:1979-05-01 00:00:00
abstract::LH treatment of isolated medium (3- to 7-mm) follicles from porcine ovaries stimulated pregnenolone synthesis by mitochondria isolated from these tissues. This LH stimulation of pregnenolone synthesis was observed regardless of whether exogenous cholesterol was added to the mitochondrial assay system, suggesting that ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-588
更新日期:1984-02-01 00:00:00
abstract::Complementary DNA-encoding proteins with high homology to steroidogenic acute regulatory proteins (StAR) of mammals were cloned from rainbow trout head kidney and a mixture of several brook trout tissues. A cDNA encoding an MLN64 homolog was also cloned from brook trout. The C-terminal domains of rainbow trout StAR an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8672
更新日期:2002-06-01 00:00:00
abstract::We have analyzed the factors responsible for the circadian variation in rat hepatic mRNA-S14. Regulation of this sequence, which is found in lipogenic tissues and encodes a protein (S14) believed to be associated with fatty acid synthesis, is an excellent model of the interaction of thyroid hormone and dietary factors...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1563
更新日期:1987-04-01 00:00:00
abstract::The insulin-like growth factor I (IGF-I) receptor is a transmembrane tyrosine kinase that mediates the growth-promoting effects of IGF-I and IGF-II. Changes in IGF-I receptor messenger RNA levels are reflected in cell surface receptor number, and modulation of IGF-I receptor levels affects tumorigenicity in numerous c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7065
更新日期:1999-10-01 00:00:00
abstract::6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key modulator of glycolysis-gluconeogenesis. To gain insight into the molecular mechanism behind hormonal and nutritional regulation of PFKFB expression, we have cloned and characterized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1506
更新日期:2006-07-01 00:00:00
abstract::The expression of genes encoding key hepatic gluconeogenic enzymes, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), is regulated at the transcriptional level by a network of transcription factors and cofactors, including cAMP response element-binding protein (CREB). It has been ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0641
更新日期:2010-02-01 00:00:00
abstract::Previous immunohistochemical and in situ hybridization studies of sheep, goats, and rodents indicated that kisspeptin (KP), neurokinin B (NKB), and dynorphin A (DYN) are extensively colocalized in the hypothalamic arcuate nucleus, thus providing a basis for the KP/NKB/DYN (KNDy) neuron concept; in both sexes, KNDy neu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1545
更新日期:2012-10-01 00:00:00
abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0473
更新日期:2006-09-01 00:00:00
abstract::Renal prostaglandin E2 (PGE2) synthesis is enhanced by exogenous vasopressin (AVP). To determine whether the pressor or the antidiuretic activities of AVP are related to its effect on PGE2 synthesis, AVP and the nonpressor analog, 1-deamino-8-D-arginine vasopressin (dD'AVP), were administered to the isolated perfused ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-495
更新日期:1981-02-01 00:00:00
abstract::The classical model for the mechanism of action of steroids holds that unbound receptors for steroids reside exclusively in the cytoplasmic compartment and that they undergo translocation to the nucleus when bound to steroids in a process which is temperature sensitive. We have in the past proposed that unbound recept...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1533
更新日期:1981-04-01 00:00:00
abstract::Interleukin-1 (IL-1) greatly induces osteoclast formation and stimulates bone resorption of mouse calvaria in culture. In the presence of soluble IL-6 receptor (sIL-6R), IL-6 similarly induces osteoclast formation, but the potency of IL-6 in inducing bone resorption in organ culture is weaker than that of IL-1. To stu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5818
更新日期:1998-03-01 00:00:00
abstract::Some 17 alpha-alkylated androgens used as anabolic agents, such as stanozolol (ST) and danazol (DA), have specific effects on the liver that are not exerted by testosterone. This gives rise to the possibility that a steroid-binding protein, other than the androgen receptor, could modulate the intracellular actions of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119180
更新日期:1994-03-01 00:00:00
abstract::Calcitonin (CT) and related peptides, such as CT gene-related peptide and salmon CT (sCT)-like peptide, are present in the rat nervous system and the pituitary gland, and sCT markedly inhibits basal and TRH-stimulated PRL release from anterior pituitary (AP) cells. Because TRH-induced PRL release is known to involve i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-613
更新日期:1990-08-01 00:00:00
abstract::Changes in insulin, somatostatin, and glucagon secretion during the development of obesity in rats after ventromedial hypothalamic (VMH) lesions were evaluated by measuring fasting hormone levels and their secretion from the isolated perfused pancreas. Fasting peripheral insulin levels were not altered 1 week after th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-457
更新日期:1984-02-01 00:00:00
abstract::Dipeptidyl peptidase-IV (DPPIV) is a serine proteinase widely distributed in mammalian tissues, including lymphocytes, where it is identical to the T-cell activation antigen, cluster differentiation antigen-26. In the present study, DPPIV enzymatic activity was found in uterine secretions of unilaterally pregnant ewes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.2.7835310
更新日期:1995-02-01 00:00:00