当前位置： SCI文献检索 > ENDOCRINOLOGY期刊下所有文献 > Peptones stimulate cholecystokinin secretion and gene transcription in the intestinal cell line STC-1.

Peptones stimulate cholecystokinin secretion and gene transcription in the intestinal cell line STC-1.

Abstract：

:In rats, protein hydrolysates (peptones) stimulate cholecystokinin (CCK) release both in vivo and in a model of isolated vascularly perfused duodeno-jejunum. However, the mechanisms involved in peptone-induced stimulation of CCK cells are not well understood. In particular, the possibility that peptones may directly interact with CCK-producing cells to stimulate CCK release and gene transcription has not yet been examined. To test this hypothesis, we used the enteroendocrine cell line STC-1. Incubation of STC-1 cells for 2 h with albumin egg hydrolysate over the concentration range 0.01-1% (wt/ vol) caused a dose-dependent release of CCK, with a maximal increase at 1420% of the control value. In contrast, BSA (1%, wt/vol) or a mixture of amino acids (1%, wt/vol) induced a modest rise in CCK secretion. A dose-dependent, hydrolysate-specific, increase in the CCK steady state RNA level was also observed. It was detectable by 2-4 h of peptone treatment and sustained until 24-48 h. Peptones did not increase the CCK RNA level in the colonic CCK-producing cell line GLUTag or in nonintestinal CCK-expressing cell lines, namely the pancreatic cell line RINm5F and the medullar thyroid carcinoma cell line CA77. The peptone-induced increase in the CCK RNA level resulted from enhanced gene transcription, because labeled CCK transcripts from nuclear run-on incubations increased 3-fold when cells were incubated with peptones, whereas the level of beta-actin transcripts was not modified. Finally, peptones dose-dependently stimulated the transcriptional activity of an 800-bp fragment of CCK gene promoter transfected in STC-1 cells. These studies indicate that peptones specifically stimulate CCK secretion and gene transcription in the intestinal cell line STC-1, and that cis-acting elements conferring peptone inducibility are located in the first 800 bp of the 5'-flanking region of the CCK gene.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Cordier-Bussat M,Bernard C,Haouche S,Roche C,Abello J,Chayvialle JA,Cuber JC

doi

10.1210/endo.138.3.5023

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

1137-44

issue

eissn

0013-7227

issn

1945-7170

journal_volume

138

pub_type

杂志文章

在线工具

Peptones stimulate cholecystokinin secretion and gene transcription in the intestinal cell line STC-1.