A putative binding site for Sp1 is involved in transcriptional regulation of CYP17 gene expression in bovine ovary.

Abstract:

:In the bovine ovary, thecal cells are the only cell type capable of expressing the CYP17 gene in response to LH. With the onset of ovulation and luteinization in the cow, there is complete loss of P450c17alpha expression. To characterize the molecular mechanisms involved in tissue-specific regulation of the CYP17 gene in the bovine ovary, deletion mutations of the bovine CYP17 promoter were ligated into a promoterless luciferase expression vector, and reporter constructs were transiently transfected into primary cultures of bovine thecal and luteal cells. Deletion of the promoter sequences between -191 and 101 bp dramatically decreased the levels of reporter gene activity in both thecal and luteal cells. Computer-assisted analysis revealed the presence of a putative inverted Sp1-like binding site at -188/-180 bp. Deletion or mutation of this sequence caused a decrease in both basal and forskolin-stimulated reporter gene activity. In addition, mutation or deletion of this sequence also decreased reporter gene expression induced by overexpression of the protein kinase A catalytic subunit. Electrophoretic mobility shift assays showed that this sequence binds to a nuclear protein(s) from both thecal and luteal cells that is related to Sp1, as suggested by the results of gel mobility supershift assay employing an antibody raised against Sp1. DNA-binding activity was not increased by the addition of forskolin to thecal or luteal cells. We conclude that this inverted Sp1-like binding sequence is involved in constitutive as well as cAMP-dependent expression of the CYP17 gene in the bovine ovary.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Borroni R,Liu Z,Simpson ER,Hinshelwood MM

doi

10.1210/endo.138.5.5112

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

2011-20

issue

5

eissn

0013-7227

issn

1945-7170

journal_volume

138

pub_type

杂志文章
  • Insulin and norepinephrine regulate ghrelin secretion from a rat primary stomach cell culture.

    abstract::Ghrelin is a peptide hormone primarily produced in the previously unidentified X/A endocrine cells of the stomach. Extensive studies have focused on the effects of ghrelin on growth hormone release and appetite regulation. However, the mechanisms regulating ghrelin secretion are less understood. In the present study, ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2012-1040

    authors: Gagnon J,Anini Y

    更新日期:2012-08-01 00:00:00

  • Serum bone gla protein and the vitamin D endocrine system in the oophorectomized rat.

    abstract::Because of the importance of estrogen in osteoporosis, the effects of decreased estrogen production using sensitive measurements of bone mineral metabolism were studied in oophorectomized rats. Serum levels of ionized calcium, bone gla protein (BGP), vitamin D metabolites (25-hydroxyvitamin D and 1,25-dihydroxyvitamin...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-122-2-624

    authors: Ismail F,Epstein S,Fallon MD,Thomas SB,Reinhardt TA

    更新日期:1988-02-01 00:00:00

  • Testosterone regulation of proopiomelanocortin messenger ribonucleic acid in the arcuate nucleus of the male rat.

    abstract::GnRH regulates the secretion of LH and FSH, which stimulate the secretion of testicular hormones. Acting in a reciprocal fashion, these hormones, including testosterone and inhibin, exert a negative feedback effect on GnRH and gonadotropin secretion. Endogenous opioid peptides (EOPs) have been implicated to play a rol...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-124-4-1697

    authors: Chowen-Breed J,Fraser HM,Vician L,Damassa DA,Clifton DK,Steiner RA

    更新日期:1989-04-01 00:00:00

  • Oxyntomodulin inhibits food intake in the rat.

    abstract::Oxyntomodulin is derived from proglucagon processing in the intestine and the central nervous system. To date, no role in the central nervous system has been demonstrated. We report here that oxyntomodulin inhibits refeeding when injected intracerebroventricularly and into the hypothalamic paraventricular nucleus of 2...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.142.10.8430

    authors: Dakin CL,Gunn I,Small CJ,Edwards CM,Hay DL,Smith DM,Ghatei MA,Bloom SR

    更新日期:2001-10-01 00:00:00

  • Rottlerin inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes by uncoupling mitochondrial oxidative phosphorylation.

    abstract::There is increasing evidence that protein kinase C (PKC) isoforms modulate insulin-signaling pathways in both positive and negative ways. Recent reports have indicated that the novel PKCdelta mediates some of insulin's actions in muscle and liver cells. Many studies use the specific inhibitor rottlerin to demonstrate ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-220259

    authors: Kayali AG,Austin DA,Webster NJ

    更新日期:2002-10-01 00:00:00

  • Kisspeptin, neurokinin B, and dynorphin act in the arcuate nucleus to control activity of the GnRH pulse generator in ewes.

    abstract::Recent work has led to the hypothesis that kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the arcuate nucleus play a key role in GnRH pulse generation, with kisspeptin driving GnRH release and neurokinin B (NKB) and dynorphin acting as start and stop signals, respectively. In this study, we tested this hypothesis...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2013-1331

    authors: Goodman RL,Hileman SM,Nestor CC,Porter KL,Connors JM,Hardy SL,Millar RP,Cernea M,Coolen LM,Lehman MN

    更新日期:2013-11-01 00:00:00

  • 19-Nor progesterone is a mineralocorticoid agonist.

    abstract::19-Nor progesterone (19-nor P) has previously been shown to have a approximately 3-fold higher affinity for mineralocorticoid receptors than progesterone (P), its C-19 methylated parent steroid; in contrast, 19-nor aldosterone has less than 1% the mineralocorticoid receptor affinity of aldosterone. In the present stud...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-109-1-313

    authors: Funder JW,Adam WR

    更新日期:1981-07-01 00:00:00

  • Unmasking of neuropeptide-Y inhibitory effects on in vitro gonadotropin secretion from pituitaries of metestrous, but not proestrous, rats.

    abstract::We have recently demonstrated that GnRH-stimulated gonadotropin secretion is augmented by coadministration with neuropeptide-Y (NPY) in anterior pituitaries removed in the afternoon from proestrous, but not metestrous, rats. To test the hypothesis that these effects of NPY are due to an interaction with progesterone (...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.136.1.7828530

    authors: Knox KL,Bauer-Dantoin AC,Levine JE,Schwartz NB

    更新日期:1995-01-01 00:00:00

  • Short ghrelin peptides neither displace ghrelin binding in vitro nor stimulate GH release in vivo.

    abstract::Ghrelin is an acylated peptide recently isolated from rat stomach that potently stimulates GH release in vitro and in vivo in rat and man. Ghrelin specifically activates the receptor for the growth hormone secretagogues (GHS-Rla), and it has been proposed as the endogenous ligand mimicked by these synthetic compounds....

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.143.5.8894

    authors: Torsello A,Ghe' C,Bresciani E,Catapano F,Ghigo E,Deghenghi R,Locatelli V,Muccioli G

    更新日期:2002-05-01 00:00:00

  • Effect of aging on the dissociation kinetics and estradiol receptor nuclear interactions in mouse uteri: correlation with biological effects.

    abstract::The dissociation kinetics of uterine estradiol receptor (UER) from young, middle-aged, and old mice have been studied in vitro and correlated with interactions of the steroid receptor with nuclear suspensions in a cell-free system. Furthermore, we have determined in these various age groups of mice the concentration o...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-118-2-750

    authors: Belisle S,Bellabarba D,Lehoux JG,Robel P,Baulieu EE

    更新日期:1986-02-01 00:00:00

  • Maternal modulation of growth hormone secretion in the neonatal rat. I. Involvement of milk factors.

    abstract::The present experiments investigated the time course of maternal modulation of GH secretion and examined the possible role of milk in the regulation of GH secretion in neonatal rats. Serum GH concentrations in neonatal rats were high at birth and declined over time postpartum. Separation of rat pups from their mothers...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-125-3-1326

    authors: Kacsoh B,Terry LC,Meyers JS,Crowley WR,Grosvenor CE

    更新日期:1989-09-01 00:00:00

  • Correlation between suppression of c-myc and antiproliferative effect of transforming growth factor-beta 1 in thyroid carcinoma cell growth.

    abstract::The growth regulatory activity of transforming growth factor-beta 1 (TGF beta 1) was studied in a clonal strain of thyroid papillary carcinoma cell (NPA). Despite the presence of TGF beta 1 and its receptor messenger RNA in thyroid carcinoma, the molecular mechanism of TGF beta 1 action on cell growth of thyroid carci...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.135.4.7925100

    authors: Usa T,Tsukazaki T,Namba H,Ohtsuru A,Kimura H,Villadolid MC,Nagataki S,Yamashita S

    更新日期:1994-10-01 00:00:00

  • Sequential patterns of circulating LH and FSH in female sheep during the early postnatal period: effect of gonadectomy.

    abstract::To determine the basis for the marked daily variability in circulating luteinizing hormone (LH) in the female lamb shortly after birth and to determine whether circulating follicle-stimulating hormone (FSH) exhibits similar variability during this period of life, serum levels of both gonadotropins were monitored frequ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-96-1-15

    authors: Foster DL,Jaffe RB,Niswender GD

    更新日期:1975-01-01 00:00:00

  • D-Trp-6-luteinizing hormone-releasing hormone inhibits hyperprolactinemia in female rats.

    abstract::The effect of a potent agonistic LHRH analog D-Trp-6-LHRH on the hyperprolactinemia induced by haloperidol was tested in intact and ovariectomized female rats. The administration of D-Trp-6-LHRH at two dose levels (5 and 50 micrograms/day) for 20 days blocked the increase in serum PRL induced by haloperidol in intact ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-116-6-2227

    authors: Debeljuk L,Torres-Alemán I,Schally AV

    更新日期:1985-06-01 00:00:00

  • The impact of the nonpeptide corticotropin-releasing hormone antagonist antalarmin on behavioral and endocrine responses to stress.

    abstract::The nonpeptide CRH antagonist antalarmin has been shown to block both behavioral and endocrine responses to CRH. However, it's potential activity in blunting behavioral and endocrine sequelae of stressor exposure has not been assessed. Because antagonism of central CRH by alpha-helical CRH attenuates conditioned fear ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.140.1.6415

    authors: Deak T,Nguyen KT,Ehrlich AL,Watkins LR,Spencer RL,Maier SF,Licinio J,Wong ML,Chrousos GP,Webster E,Gold PW

    更新日期:1999-01-01 00:00:00

  • Hyperparathyroidism is associated with reduced expression of a parathyroid calcium receptor mechanism defined by monoclonal antiparathyroid antibodies.

    abstract::Parathyroid tissue from patients with hyperparathyroidism (HPT) exhibited reduced immunohistochemical reactivity with monoclonal antiparathyroid antibodies, previously shown to stain intensely the surface of normal human parathyroid cells and to interfere with a receptor mechanism of these cells which is involved in t...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-122-6-2999

    authors: Juhlin C,Klareskog L,Nygren P,Ljunghall S,Gylfe E,Rastad J,Akerström G

    更新日期:1988-06-01 00:00:00

  • Modulation by the hypothalamus of glucagon and insulin secretion in rabbits: studies with electrical and chemical stimulations.

    abstract::The effects of electrical and chemical stimulations of the hypothalamus on the plasma levels of glucagon, insulin, and glucose were studied in undisturbed and free-moving rabbits. Electrical stimulation of the ventromedial hypothalamic nucleus (VMH) caused a marked increase in the circulating level of glucagon coincid...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-108-2-605

    authors: Shimazu T,Ishikawa K

    更新日期:1981-02-01 00:00:00

  • Muscarinic regulation of intracellular signaling and neurosecretion in gonadotropin-releasing hormone neurons.

    abstract::Agonist activation of cholinergic receptors expressed in perifused hypothalamic and immortalized GnRH-producing (GT1-7) cells induced prominent peaks in GnRH release, each followed by a rapid decrease, a transient plateau, and a decline to below basal levels. The complex profile of GnRH release suggested that acetylch...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.139.10.6267

    authors: Krsmanovic LZ,Mores N,Navarro CE,Saeed SA,Arora KK,Catt KJ

    更新日期:1998-10-01 00:00:00

  • Regulation of Kiss1 expression by sex steroids in the amygdala of the rat and mouse.

    abstract::Kisspeptin (encoded by the Kiss1 gene) is an important regulator of reproduction. In rodents, Kiss1 is expressed in two hypothalamic regions, the arcuate nucleus and anteroventral periventricular/ periventricular continuum, where it is regulated by sex steroids. However, the distribution, regulation, and functional si...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2010-1498

    authors: Kim J,Semaan SJ,Clifton DK,Steiner RA,Dhamija S,Kauffman AS

    更新日期:2011-05-01 00:00:00

  • Production and measurement of exophthalmos-producing factor in guinea pigs.

    abstract::Although the extent of proptosis in exophthalmic Graves' disease has been measured directly and shown to correlate with serum content of a bioassayable exophthalmus-producing factor (EPS;1), a comparable relationship in an experimental model has not been reported. Progressive exophthalmos, measured from photographs an...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-96-1-151

    authors: Valk TW,Taylor RE Jr,Barker SB

    更新日期:1975-01-01 00:00:00

  • Urocortin-related peptides increase interleukin-6 output via cyclic adenosine 5'-monophosphate-dependent pathways in A7r5 aortic smooth muscle cells.

    abstract::Corticotropin-releasing factor receptor type 2beta, expressed in the rodent cardiovascular system, is a member of the G protein-coupled receptor family. This receptor is coupled positively to adenylate cyclase and is bound preferentially by the urocortin (Ucn)-related peptides (Uncs): Ucn, Ucn II, and Ucn III. In the ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-0023

    authors: Kageyama K,Suda T

    更新日期:2003-06-01 00:00:00

  • Inhibitory GH receptor extracellular domain monoclonal antibodies: three-dimensional epitope mapping.

    abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2011-1336

    authors: Jiang J,Wan Y,Wang X,Xu J,Harris JM,Lobie PE,Zhang Y,Zinn KR,Waters MJ,Frank SJ

    更新日期:2011-12-01 00:00:00

  • A novel extracellular nucleotide receptor coupled to phosphoinositidase-C in pituitary cells.

    abstract::In primary cultures of sheep pituitary cells extracellular nucleotides stimulated rapid increases in inositol tris- and bisphosphate, accompanied by intracellular Ca2+ mobilization. A similar stimulation of inositol phosphate production by extracellular nucleotides was observed in rat and baboon pituitary cells. The i...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-126-1-80

    authors: Davidson JS,Wakefield IK,Sohnius U,van der Merwe PA,Millar RP

    更新日期:1990-01-01 00:00:00

  • Xylazine-induced hyperglycemia in cattle: a possible involvement of alpha 2-adrenergic receptors regulating insulin release.

    abstract::The mechanism by which xylazine raises plasma glucose but lowers insulin concentrations was examined in Holstein cows. Intravenous injection of xylazine (15-150 micrograms/kg) induced a dose-dependent hyperglycemia and hypoinsulinemia for 3-4 h. An alpha 2-adrenergic blocking agent, yohimbine, and an alpha 1- and alph...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-109-3-825

    authors: Hsu WH,Hummel SK

    更新日期:1981-09-01 00:00:00

  • Functional coupling of neonatal rat Sertoli cells and gonocytes in coculture.

    abstract::Interaction between Sertoli cells and germ cells is likely to be critical for normal development of the testis. We have established and characterized cocultures of neonatal Sertoli cells and gonocytes and have begun to study the physical and functional relationship between these cells in vitro. Cells were isolated fro...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-127-6-2812

    authors: Orth JM,Boehm R

    更新日期:1990-12-01 00:00:00

  • Differential patterns of expression of DAX-1 and steroidogenic factor-1 (SF-1) in the fetal rat testis.

    abstract::Gonadal differentiation is dependent upon a cascade of molecular and morphological events. Steroidogenic factor 1 (SF-1) and DAX-1 have been implicated in this process. A SF-1 binding site has been reported to be present on the DAX-1 gene. We therefore used immunocytochemistry to determine whether these two transcript...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.137.8.8754790

    authors: Majdic G,Saunders PT

    更新日期:1996-08-01 00:00:00

  • Ca2+ Channels in Anterior Pituitary Somatotrophs: A Therapeutic Perspective.

    abstract::Ca2+ influx through voltage-gated Ca2+ channels (VGCCs) plays a key role in GH secretion. In this review, we summarize the current state of knowledge regarding the physiology and molecular machinery of VGCCs in pituitary somatotrophs. We next discuss the possible involvement of Ca2+ channelopathies in pituitary diseas...

    journal_title:Endocrinology

    pub_type: 杂志文章,评审

    doi:10.1210/en.2018-00743

    authors: Nussinovitch I

    更新日期:2018-12-01 00:00:00

  • Pituitary regulation of postnatal small intestinal ontogeny in the rat: differential regulation of digestive hydrolase maturation by thyroxine and growth hormone.

    abstract::During the third week of postnatal life, dramatic ontogenic changes occur in the morphology and enzymology of the small intestine of the infant rat, enabling the animal to make the transition from milk to solid food. To investigate the roles of T4 and GH in regulation of these changes, infant rats were hypophysectomiz...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-129-3-1417

    authors: Castillo RO,Glasscock GF,Noren KM,Reisenauer AM

    更新日期:1991-09-01 00:00:00

  • Autoregulation of pituitary growth hormone messenger ribonucleic acid levels in rats bearing transplantable mammosomatotrophic pituitary tumors.

    abstract::Female Wistar-Furth rats were implanted sc with GH3 rat pituitary tumor cells. Tumors were palpable by 4 weeks, and animals were killed periodically from 5-9 weeks. Tumor-bearing rats (n = 10) were heavier than their respective controls, reaching a weight of 372 +/- 3 by 9 weeks vs. 195 +/- 5 g in controls (mean +/- S...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-118-3-915

    authors: Yamashita S,Slanina S,Kado H,Melmed S

    更新日期:1986-03-01 00:00:00

  • beta-Endorphin secretion of arginine vasopressin in vivo.

    abstract::The effect of beta-endorphin upon plasma arg8-vasopressin release was studied in vivo in rabbits and in vitro in rat neural lobes. Following intravenous administration of 200 mug/kg synthetic beta-endorphin plasma AVP rose significantly by five minutes after injection and remained elevated for twenty-five minutes comp...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-101-5-1643

    authors: Weitzman RE,Fisher DA,Minick S,Ling N,Guillemin R

    更新日期:1977-11-01 00:00:00