Abstract:
:We studied the involvement of the cAMP pathway in the regulation of beta TC1 cell growth with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the activator of adenylate cyclase forskolin. We examined the effect of the increase in cAMP content on the serum-induced resumption of the cell cycle of quiescent cells. IBMX and forskolin both inhibited the mitogenic effect of serum in a concentration-dependent manner. Intracellular cAMP levels were, respectively, enhanced 3.0- and 8.6-fold by IBMX (0.5 mM) and forskolin (20 microM) within 1 h. IBMX and forskolin were also inducers of insulin release, indicating that the growth-arrested beta TC1 cells have retained the essential characteristics of the normal differentiated beta-cells. The effects of IBMX and forskolin were correlated with a modulation of cell cycle-related gene expression. IBMX induced expression of the c-fos gene, which was further enhanced by the simultaneous addition of serum, whereas forskolin alone elicited maximal induction of this gene. Interestingly, c-jun expression was only enhanced with forskolin. We also studied the effects of IBMX and forskolin on the expression of the simian virus-40 T-antigen controlled by the rat insulin II promoter in beta TC1 cells. IBMX and forskolin inhibited the serum-induced accumulation of simian virus-40 T-antigen mRNA in quiescent as well as exponentially growing beta TC1 cells.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lavergne C,Breant B,Rosselin Gdoi
10.1210/endo.131.5.1385100subject
Has Abstractpub_date
1992-11-01 00:00:00pages
2351-6issue
5eissn
0013-7227issn
1945-7170journal_volume
131pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::System A is one of the most highly regulated transport systems for transport of neutral amino acids into mammalian cells. Stimulation of uptake of alpha-[3H]methylaminoisobutyric acid (MeAIB), a nonmetabolizable system A substrate, by a novel insulin-sensitizing agent, troglitazone, in 3T3-L1 adipocytes was investigat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5795
更新日期:1998-03-01 00:00:00
abstract::Contrary to widespread belief, the implantation of an embryo for the initiation of pregnancy is like a battle, in that the embryo uses a variety of coercive tactics to force its acceptance by the endometrium. We propose that embryo implantation involves a three-step process: (1) identification of a receptive endometri...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2017-03082
更新日期:2018-02-01 00:00:00
abstract::Osteocalcin (OC), a bone specific protein expressed during differentiation and mineralization of the bone extracellular matrix, is down-regulated upon treatment with transforming growth factor (TGF)-beta 1. To address the potential role of OC gene expression in relation to TGF-beta 1 regulation of bone formation and r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612540
更新日期:1996-05-01 00:00:00
abstract::Mutations in the PCSK1 gene encoding prohormone convertase 1/3 (PC1/3) are strongly associated with obesity in humans. The PC1/3(N222D) mutant mouse thus far represents the only mouse model that mimics the PC1/3 obesity phenotype in humans. The present investigation addresses the cell biology of the N222D mutation. Me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1985
更新日期:2014-07-01 00:00:00
abstract::We sequenced the complementary DNA (cDNA) encoding guinea pig LHRH from an expression library derived from the preoptic area-anterior hypothalamus. Data from in situ hybridization and RNase protection assays verified that the cloned cDNA was complementary to guinea pig LHRH messenger RNA. The architecture of the deduc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5454
更新日期:1997-10-01 00:00:00
abstract::A single injection of either natural (cortisol, corticosterone) or synthetic [dexamethasone (DEX), triamcinolone acetonide] glucocorticosteroids to estradiol-primed, withdrawn chicks, resulted in a dose-dependent increase in the relative rates of ovalbumin and conalbumin synthesis. The simultaneous injection of equal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2384
更新日期:1985-06-01 00:00:00
abstract::Commercially available preparations of natural porcine glucose-dependent insulinotropic polypeptide (GIP) were subjected to reverse phase HPLC. The material was found to give rise to 4 peaks which were characterized by HPLC-retention time and N-terminal sequence analysis. They represented: intact porcine GIP(1-42), 58...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-835
更新日期:1987-02-01 00:00:00
abstract::The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1168
更新日期:1985-03-01 00:00:00
abstract::Type I iodothyronine 5' deiodinase (5'DI) contains selenocysteine, encoded by a UGA codon, and this amino acid is essential for maximum catalytic efficiency in this enzyme. We recently showed that translation of UGA as selenocysteine in this protein requires a specific sequence of about 250 nucleotides in the 3' untra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1396330
更新日期:1992-10-01 00:00:00
abstract::Women with type 1 diabetes are subfertile. Diabetes negatively affects pregnancy by causing early miscarriage and poor prenatal outcomes. In this study we examine consequences of maternal type 1 diabetes on early embryo development, metabolic gene expression, and the pattern of insulin receptor (IR) and IGF-I receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0187
更新日期:2010-09-01 00:00:00
abstract::Estrogens have been shown to have positive and negative effects on anxiety and depressive-like behaviors, perhaps explained by the existence of two distinct estrogen receptor (ER) systems, ERalpha and ERbeta. The ERbeta agonist, diarylpropionitrile (DPN) has been shown to have anxiolytic properties in rats. DPN exists...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1355
更新日期:2009-04-01 00:00:00
abstract::Pheochromocytomas (PCCs) are tumors arising from neural crest-derived chromaffin cells. There are currently few animal models of PCC that recapitulate the key features of human tumors. Because such models may be useful for investigations of molecular pathomechanisms and development of novel therapeutic interventions, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1108
更新日期:2016-08-01 00:00:00
abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-2014
更新日期:1981-05-01 00:00:00
abstract::Adipose tissue secretes a variety of bioactive signaling molecules, termed adipokines, which regulate numerous biological functions including appetite, energy balance, glucose homeostasis, and inflammation. Chemerin is a novel adipokine that regulates adipocyte differentiation and metabolism by binding to and activati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1490
更新日期:2012-02-01 00:00:00
abstract::Alpha-calcitonin gene-related peptide (alphaCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary physiological role has thus far remained obscure. Increased lipid content, particularly in skeletal muscle and liver, is ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0583
更新日期:2008-01-01 00:00:00
abstract::In this study, site-directed mutagenesis and biochemical strategies have been used to establish whether disulfide bonding between extracellular Cys residues contributes to the structural integrity of the GnRH receptor (GnRH-R) and, if so, to delineate the nature of the bonding patterns involved. The majority of G prot...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5233
更新日期:1997-07-01 00:00:00
abstract::Studies show that a subset of transgender men desire children; however, there is a paucity of literature on the effect of gender-affirming testosterone therapy on reproductive function. In this manuscript, we will review the process of gender-affirming hormone therapy for transgender men and what is known about ovaria...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa014
更新日期:2020-03-01 00:00:00
abstract::The recently discovered estrogen receptor-beta (ERbeta) is expressed in rodent and human testes. To obtain insight in the physiological role of ERbeta we have investigated the cell type-specific expression pattern of ERbeta messenger RNA (mRNA) and protein in the testis of rats of various ages by in situ hybridization...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6438
更新日期:1999-01-01 00:00:00
abstract::Sodium vanadate, an agent known to have multiple cellular actions, was studied for its effects on aspects of bone formation in cultures of 21-day-old fetal rat calvariae. Vanadate (0.1-10 microM) stimulated the incorporation of [3H] thymidine into acid-insoluble residues (DNA); the effect appeared after 3 h and was su...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-855
更新日期:1985-03-01 00:00:00
abstract::Even though functional CRH receptors have been identified in several brain regions by ligand binding, the identity of brain areas expressing the CRH receptor gene has not been described. The recent cloning of the rat CRH receptor gene has permitted us to conduct an in situ hybridization histochemistry study to localiz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7956950
更新日期:1994-11-01 00:00:00
abstract::In the spiny dogfish (Squalus acanthias), germ cells and adjacent steroidogenic elements are topographically segregated within the testis according to stage of development. In the experiments reported here, we have taken advantage of this favorable anatomical arrangement to demonstrate stage-specific variations in ste...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1328
更新日期:1985-10-01 00:00:00
abstract::Complementary DNAs encoding two forms of cocaine- and amphetamine-regulated transcript (CART) peptide precursors were identified from goldfish brain and named CART I and CART II. Each cDNA contains a signal peptide sequence, the putative CART-like peptide, and a carboxy-terminal extension peptide. Form I encodes a 117...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8519
更新日期:2001-12-01 00:00:00
abstract::The fragile X syndrome is the most frequent hereditary form of mental retardation. This X-linked disorder is, in most cases, caused by an unstable and expanding trinucleotide CGG repeat located in the 5'-untranslated region of the gene involved, the fragile X mental retardation 1 (FMR1) gene. Expansion of the CGG repe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5706
更新日期:1998-01-01 00:00:00
abstract::We have investigated the adrenergic control of mitogen-activated protein kinase (MAPK) activity in brown adipocytes. Cold exposure in rats led to an activation of MAPK in brown adipose tissue, as determined by the gel mobility shift assay and in-gel kinase assay. In contrast, no activation was seen after surgical symp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.1.4832
更新日期:1997-01-01 00:00:00
abstract::The effect of 3 alpha-hydroxy-4-pregnen-20-one (3HP), a Sertoli cell steroid, on the secretion of gonadotropins from rat anterior pituitary cells in culture was examined and subsequently compared with the action of other gonadal steroids and steroids structurally related to 3HP. Pituitary cells from randomly cycling, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-41
更新日期:1989-07-01 00:00:00
abstract::Chemerin is an adipokine with important regulatory roles in adipogenesis. In humans, serum total chemerin (i.e. prochemerin plus chemerin) levels are positively associated with body mass index and metabolic syndrome. However, the mechanisms that increase serum chemerin concentration are unknown. We hypothesized that c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0794
更新日期:2010-06-01 00:00:00
abstract::Recently, heterogeneity of POMC mRNA content between intermediate lobe melanotropes of the rat pituitary gland was demonstrated by in situ hybridization of tissue sections. In the present study the heterogeneity of POMC mRNA content in dispersed rat pituitary cells has been investigated. Acutely dispersed cells from a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1359
更新日期:1989-03-01 00:00:00
abstract::Leptin, a recently-discovered hormonal product of the obese gene, circulates in the blood at levels paralleling those of fat reserves and regulates satiety and improves reproductive performance if injected into mice lacking circulating leptin. Therefore, we tested the hypothesis that leptin signals metabolic informati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5311
更新日期:1997-08-01 00:00:00
abstract::ACTH produced a 75% increase in pregnenolone biosynthesis from endogenous precursors in isolated cells prepared from the rat Snell adrenocortical carcinoma 494. On the addition of 24- and 25-hydroxycholesterol to the tumor cells, the rate of pregnenolone synthesis increased 10-fold but was insensitive to the presence ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-5-1230
更新日期:1979-11-01 00:00:00
abstract::Impaired wound healing of skin is one of the most serious complications of diabetes. However, the pathogenesis of this process is not known, and it is unclear whether impaired insulin signaling could directly affect skin physiology. To elucidate the role of insulin in skin, we studied skin insulin receptor (IR) null m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.7988
更新日期:2001-03-01 00:00:00