当前位置： SCI文献检索 > ENDOCRINOLOGY期刊下所有文献 > Commercially available preparations of porcine glucose-dependent insulinotropic polypeptide (GIP) contain a biologically inactive GIP-fragment and cholecystokinin-33/-39.

Commercially available preparations of porcine glucose-dependent insulinotropic polypeptide (GIP) contain a biologically inactive GIP-fragment and cholecystokinin-33/-39.

Abstract：

:Commercially available preparations of natural porcine glucose-dependent insulinotropic polypeptide (GIP) were subjected to reverse phase HPLC. The material was found to give rise to 4 peaks which were characterized by HPLC-retention time and N-terminal sequence analysis. They represented: intact porcine GIP(1-42), 58% (wt/wt); the GIP-fragment des-tyr-ala-GIP(3-42), 32% (wt/wt); cholecystokinin (CCK)-33 2% (wt/wt); CCK-39 2% (wt/wt). HPLC-pure GIP(1-42) stimulated insulin release in rat isolated pre-cultured pancreatic islets in the presence of 16.7 mM glucose up to 240% vs. control, whereas the fragment des-tyr-ala-GIP(3-42) did neither increase insulin release nor exhibit antagonistic activity to GIP(1-42) at 100 ng/ml. These results indicate that commercially available porcine GIP-preparations may contain the biologically inactive des-tyr-ala-GIP(3-42) in high amounts, and in addition may be contaminated by CCK-peptides. HPLC-characterization of these peptide preparations prior to any biological study is crucial.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Schmidt WE,Siegel EG,Kümmel H,Gallwitz B,Creutzfeldt W

doi

10.1210/endo-120-2-835

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

835-7

issue

2

eissn

0013-7227

issn

1945-7170

journal_volume

120

pub_type

杂志文章

相关文献

ENDOCRINOLOGY文献大全