Abstract:
:The rat esophagus shares some cellular features with skin squamous epithelium and striated muscle that express high levels of corticotropin-releasing factor type 2 (CRF2) receptors or their cognate ligand urocortin (Ucn) 1, 2, and 3. We investigated the expression and cell signaling of CRF2 receptors and ligands in the rat esophagus and lower esophageal sphincter (LES) by RT-PCR and quantitative PCR in normal and corticosterone-treated whole esophageal tissue, laser capture microdissected layers, and isolated esophageal cells. The expression of CRF2 receptor protein and intracellular cAMP and ERK1/2 responses to CRF agonists and CRF2 antagonist were determined in cultured esophageal cells and HEK-293 cells transfected with CRF2b receptors. CRF2 was abundantly expressed in the mucosa and longitudinal muscle layers of the esophagus and LES, whereas CRF1 expression was scarce. CRF2b wild-type transcript was predominantly expressed in the esophagus, and in addition, several new CRF2 splice variants including six CRF2a isoforms were identified. Expression of Ucn 1, Ucn 2, and to a smaller extent Ucn 3, but not CRF mRNA, was detected in the esophagus and LES. Ucn 1 and Ucn 2 stimulated dose-dependent cAMP production and ERK1/2 phosphorylation in the esophageal cells, whereas CRF and CRF1 agonist, cortagine, had less potent effects. In addition, Ucn 2-stimulated cAMP and ERK responses were blocked by the CRF2 antagonist, astressin2-B. These data established the presence of a prominent CRF2 signaling system in the esophagus and LES-encompassing multiple CRF2 receptor variants and Ucn, suggesting a functional role in secretomotor activity and epithelial and muscle cell proliferation.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Wu SV,Yuan PQ,Wang L,Peng YL,Chen CY,Taché Ydoi
10.1210/en.2006-0565subject
Has Abstractpub_date
2007-04-01 00:00:00pages
1675-87issue
4eissn
0013-7227issn
1945-7170pii
en.2006-0565journal_volume
148pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Complementary DNAs encoding two forms of cocaine- and amphetamine-regulated transcript (CART) peptide precursors were identified from goldfish brain and named CART I and CART II. Each cDNA contains a signal peptide sequence, the putative CART-like peptide, and a carboxy-terminal extension peptide. Form I encodes a 117...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8519
更新日期:2001-12-01 00:00:00
abstract::To study blood capillary growth in the hyperplastic thyroid gland, rats were fed 0.25% thiouracil in a low iodine diet for time intervals up to 100 days. Thyroids were fixed by vascular perfusion and embeded in Epon. Whole lobes were sectioned from pole to pole and slides were prepared of sections every 0.3 mm. Capill...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2306
更新日期:1978-12-01 00:00:00
abstract::Six pony mares were ovariectomized (OVX) on day 16 of diestrus during June and July, 1972, to study short term changes in plasma luteinizing hormone (LH) concentrations. Plasma LH was higher (P less than .05) 3 days after OVX (1.76 ng/ml) than the day after OVX (1.01 ng/ml), and a gradual increase occurred over the fi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-4-958
更新日期:1976-04-01 00:00:00
abstract::Estrogens have been implicated in the regulation of prolactin gene expression in man, although previous studies have not defined the molecular mechanism whereby estradiol activates the human prolactin gene promoter (hPrl). We found that estradiol induced a reproducible 1.8-fold activation of the hPrl gene promoter, us...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1066
更新日期:2008-02-01 00:00:00
abstract::The effects of insulin treatment and dietary glucose on the responsiveness of adipose tissue to insulin and GH after hypophysectomy were studied. Male rats, 130-150 g, were hypophysectomized. Glucose metabolism was measured by determining the production of CO2 from [14C]glucose and the incorporation of glucose into li...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-945
更新日期:1985-03-01 00:00:00
abstract::Steroidogenic acute regulatory protein (StAR) synthesis and steroidogenesis are stimulated by activation of divergent signaling pathways in the adrenal cortex. The two major physiological regulators of aldosterone synthesis in the adrenal zona glomerulosa are angiotensin II (AII) and extracellular K+, which both media...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7085
更新日期:1999-10-01 00:00:00
abstract::Insulin coordinates the complex response to feeding, affecting numerous metabolic and hormonal pathways. Forkhead box protein O1 (FoxO1) is one of several signaling molecules downstream of insulin; FoxO1 drives gluconeogenesis and is suppressed by insulin. To determine the role of FoxO1 in mediating other actions of i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00870
更新日期:2018-03-01 00:00:00
abstract::Like insulin-like growth factor binding protein-3 (IGFBP-3), IGFBP-5 forms a ternary complex with insulin-like growth factor (IGF)-I or IGF-II, and the acid-labile subunit (ALS). The study of IGFBP-5/IGFBP-6 chimeric proteins with amino-terminal and middle domain swaps, has revealed the existence of a site in the midd...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7375
更新日期:2000-01-01 00:00:00
abstract::Marked development of the mammary parenchyma (ducts, lobules, and alveoli) occurs during the last third of the 115-day gestation period in the gilt. These changes in the mammary gland are temporally correlated with elevated blood levels of relaxin, estrogen, and progesterone. We recently demonstrated that relaxin play...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070370
更新日期:1994-09-01 00:00:00
abstract::The effect of synthetic atrial natriuretic factor (ANF) on adrenal steroidogenesis has been studied in primary culture of bovine adrenal cells. ANF-(8-33) produced a potent 40-70% inhibition of angiotensin II-, ACTH-, PGE1-, and forskolin-stimulated secretion of aldosterone production from zona glomerulosa cells with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1636
更新日期:1984-10-01 00:00:00
abstract::Leptin regulates food intake and other activities through its hypothalamic receptor. Leptin receptors are also found in other organs, including the ovary. Direct effects of leptin in ovarian steroid production were studied in primary rat granulosa cells and in rat and human granulosa cell lines. Leptin (0.6-18 nM) sup...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6614
更新日期:1999-04-01 00:00:00
abstract::Carbohydrate metabolism in pregnancy reflects the balance between counterregulatory hormones, which induce insulin resistance, and lactogenic hormones, which stimulate beta-cell proliferation and insulin production. Here we explored the interactions of prolactin (PRL) and glucocorticoids in the regulation of beta-cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0051
更新日期:2008-11-01 00:00:00
abstract::We have previously demonstrated in vitro that 1alpha,25-dihydroxyvitamin D3 (calcitriol) treatment increases p27 expression and decreases cell proliferation in cultured thyroid carcinoma cell lines. We hypothesized that in vivo treatment with calcitriol would have a beneficial effect on thyroid carcinoma growth and pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0785
更新日期:2004-12-01 00:00:00
abstract::Fibrosis (extracellular matrix accumulation) is the final end point in diabetic cardiomyopathy. The current study evaluated the therapeutic effects of the antifibrotic hormone relaxin (RLX) in streptozotocin-treated transgenic mRen-2 rats, which undergo pathological and functional features similar to human diabetes. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0250
更新日期:2008-07-01 00:00:00
abstract::Interaction between Sertoli cells and germ cells is likely to be critical for normal development of the testis. We have established and characterized cocultures of neonatal Sertoli cells and gonocytes and have begun to study the physical and functional relationship between these cells in vitro. Cells were isolated fro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-2812
更新日期:1990-12-01 00:00:00
abstract::Mouse β-cell-specific reporter lines have played a key role in diabetes research. Although the rat provides several advantages, its use has lagged behind the mouse due to the relative paucity of genetic models. In this report we describe the generation and characterization of transgenic rats expressing a Renilla lucif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1550
更新日期:2016-03-01 00:00:00
abstract::The Kiss1 gene and its product kisspeptin are important regulators of reproduction. In rodents, Kiss1 is expressed in the hypothalamic arcuate (ARC) and anteroventral periventricular (AVPV)/rostral periventricular (PeN) nuclei. In the AVPV/PeN, females have more Kiss1 and tyrosine hydroxylase (TH) neurons than males. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0783
更新日期:2010-12-01 00:00:00
abstract::The gastrointestinal tract of the pig undergoes enhanced growth as well as morphological and functional differentiation during the perinatal period. Concurrently, porcine neonates ingest physiologically significant amounts of insulin-like growth factor-I (IGF-I) via colostrum and milk. The objectives of this study wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-1125
更新日期:1990-02-01 00:00:00
abstract::To determine in neonates the effects of protein restriction on growth, serum IGF-I, and IGF-I gene expression, we adapted a technique for rearing neonatal rats (days 6-18 of life) artificially by continuous infusion of milk through a gastrostomy. The artificially reared (AR) animals were given isocaloric diets contain...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2368
更新日期:1989-11-01 00:00:00
abstract::AtT20/D16v is a clonal strain of mouse pituitary tumor cells which synthesizes and secretes ACTH. Somatostatin, a hypothalamic tetradecapeptide, has been shown to inhibit the release of PRL, GH, and TSH from the pituitary gland. We have characterized specific binding sites for somatostatin on AtT20/D16v cells and demo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-281
更新日期:1981-01-01 00:00:00
abstract::Both starvation and feeding of a low protein diet have dramatic effects on serum thyroid hormone concentrations and on the serum binding proteins for thyroid hormones in rats. We examined whether similar changes might be seen in another model of undernutrition, namely underfeeding without alteration of dietary composi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2464
更新日期:1986-06-01 00:00:00
abstract::Up to now, no relevant tumor antigen has been identified in medullary thyroid carcinoma (MTC). The aim of the present study was to prove the concept of an immunization with an amino acid-modified calcitonin (CT) for the treatment of MTC in a transgenic mouse model. Amino acid-modified (human) CT has been chosen for va...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0631
更新日期:2008-11-01 00:00:00
abstract::The effects of morphine on the release of dopamine from tuberoinfundibular neurons and the release of PRL from the anterior pituitary gland were studied in diestrous female rats. Morphine ions ejected iontophoretically in minute quantities into the arcuate nuclei (using one electrode per nucleus) were found to reduce ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-767
更新日期:1981-03-01 00:00:00
abstract::To study the interaction of TSH receptor (TSHR) autoantibodies with receptor protein; it is necessary first to express the receptor in the proper conformation including the formation of correct disulfide bridges. However, the reducing environment of the Escherichia coli (E. coli) cytoplasm prevents the generation of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4920
更新日期:1997-02-01 00:00:00
abstract::In preovulatory ovarian follicles, the oocyte is maintained in meiotic prophase arrest by natriuretic peptide precursor C (NPPC) and its receptor natriuretic peptide receptor 2 (NPR2). LH treatment results in the decrease of NPR2 guanylyl cyclase activity that promotes resumption of meiosis. We investigated the regula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1133
更新日期:2013-09-01 00:00:00
abstract::Oxytocin stimulates phosphoinositide turnover in myometrium. To elucidate whether the coupling mechanism involves the interaction of oxytocin receptor with GTP-binding proteins, we examined oxytocin stimulation of guanosine triphosphatase (GTPase) activity and phospholipase-C activity in rat and human myometrial membr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7895660
更新日期:1995-04-01 00:00:00
abstract::Recent observations suggest a role for interleukin-1 (IL-1), a polypeptide product of macrophage/monocytic cells, in the immune-mediated destruction of pancreatic islet beta-cells observed in type 1 diabetes. In this study, we investigated the effects of IL-1 on both alpha- and beta-cell secretory functions in rat isl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2393
更新日期:1988-06-01 00:00:00
abstract::Pharmacological amounts of 25(s),26-dihydroxyvitamin D3 were administered to normal, vitamin D-replete rats in order to assess its pharmacological activity. Treatment with 25(s),26-dihydroxyvitamin D3 (20 micrograms/day for 1 week) caused a marked and significant fall in the circulating concentration of 1,25-dihydroxy...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1671
更新日期:1987-11-01 00:00:00
abstract::Activators of peroxisome proliferator activated receptor (PPAR) regulate fatty acid metabolism and can induce adipocyte differentiation. We show here that the gamma subtype of PPAR is expressed at high levels in adipose tissue in contrast to a variety of other tissues, where little gene expression was noted. In additi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.2.8033830
更新日期:1994-08-01 00:00:00
abstract::Dopaminergic inhibition of PRL release stimulated by agents that affect cytosolic Ca2+ concentrations, C-kinase activity, and cAMP levels was studied in perifused rat anterior pituitary cells cultured on cytodex beads. We used A23187 (20 microM) to increase intracellular Ca2+, the phorbol ester 12-O-tetradecanoyl-phor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-439
更新日期:1985-08-01 00:00:00