Abstract:
:To determine in neonates the effects of protein restriction on growth, serum IGF-I, and IGF-I gene expression, we adapted a technique for rearing neonatal rats (days 6-18 of life) artificially by continuous infusion of milk through a gastrostomy. The artificially reared (AR) animals were given isocaloric diets containing 8%, 13.5%, or 18% lactalbumin protein. The AR rats were compared to rats reared by their mothers (MR) for 18 days. The growth of AR rats was related to the amount of dietary protein, with the pups given 18% protein having the best growth (25.55 g gained during the 12 days of the study) and those given 8% protein having the worst (13.42 g). The 13.5% protein-fed animals were intermediate in weight gain (18.39 g). The weight gains of the 18% and 8% protein-fed pups were significantly different from that of the MR animals (18.37 g). An identical pattern of tail length growth was noted among the groups. Mean serum IGF-I concentrations followed the same pattern (MR, 1.66 U/ml on day 18 of life; 18% AR, 2.53; 13.5% AR, 1.52; 8% AR, 1.31). Liver IGF-I mRNA was rank-ordered identically with weight gain and serum IGF-I [MR, 23.10 pg/micrograms poly(A+) RNA; 18% AR, 27.66; 13.5% AR, 21.02; 8% AR, 18.76]. Unexpectedly, the 7.5-kilobase IGF-I mRNA size class showed a 2- to 3-fold higher abundance in all groups of AR rats compared to that in MR controls (P less than 0.01), suggesting that this IGF-I size class is regulated independently of the other species. The reductions in serum IGF-I and IGF-I mRNA during protein restriction of neonatal rats suggest that these responses are mediated by decreased IGF-I gene expression at the level of transcription or RNA stabilization.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Moats-Staats BM,Brady JL Jr,Underwood LE,D'Ercole AJdoi
10.1210/endo-125-5-2368subject
Has Abstractpub_date
1989-11-01 00:00:00pages
2368-74issue
5eissn
0013-7227issn
1945-7170journal_volume
125pub_type
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