Abstract:
:The aim of this study was to determine whether bisphenol A (BPA) has adverse effects on cardiovascular functions in CD-1 mice and define sex-specific modes of BPA action in the heart. Dams and analyzed progeny were maintained on a defined diet containing BPA (0.03, 0.3, 3, 30, or 300 ppm) that resulted in BPA exposures from 4-5 to approximately 5000 μg/kg · d or a diet containing 17α-ethinyl estradiol (EE; ∼0.02, 0.2, and 0.15 μg/kg · d) as an oral bioavailable estrogen control. Assessment of electrocardiogram parameters using noninvasive methods found that ventricular functions in both male and female mice were not altered by either BPA or EE. However, exposure-related changes in the rates of ventricular contraction, suggestive of a shift in sympathovagal balance of heart rate control toward increased parasympathetic activity, were detected in males. Decreased systolic blood pressure was observed in males exposed to BPA above 5 μg/kg · d and in females from the highest BPA exposure group. Morphometric histological measures revealed sexually dimorphic changes in the composition of the cardiac collagen extracellular matrix, increases in fibrosis, and evidence of modest exposure-related remodeling. Experiments using the α-selective adrenergic agonist phenylephrine found that BPA enhanced reflex bradycardia in females, but not males, revealed that BPA and EE exposure sex specifically altered the sympathetic regulation of the baroreflex circuits. Increased sensitivity to the cardiotoxic effects of the β-adrenergic agonist isoproterenol was observed in BPA- and EE-exposed females. This effect was not observed in males, in which BPA or EE exposures were protective of isoproterenol-induced ischemic damage and hypertrophy. The results of RNA sequence analysis identified significant sex-specific changes in gene expression in response to BPA that were consistent with the observed exposure-related phenotypic changes in the collagenous and noncollagenous extracellular matrix, cardiac remodeling, altered autonomic responses, changes in ion channel and transporter functions, and altered glycolytic and lipid metabolism.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Belcher SM,Gear RB,Kendig ELdoi
10.1210/en.2014-1847subject
Has Abstractpub_date
2015-03-01 00:00:00pages
882-95issue
3eissn
0013-7227issn
1945-7170journal_volume
156pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Studies in rodents demonstrate that the mitogen, IGF-I, stimulates intestinal peptide YY (PYY) expression. To investigate whether the stimulatory influence of IGF-I is exerted at the level of gene transcription, rat PYY 5'-upstream sequences (-2800/+37 bp, -770/+37 bp, -127/+37 bp) fused to the firefly luciferase (luc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0770
更新日期:2004-02-01 00:00:00
abstract::Hypophysectomized female rats which received renal grafts of anterior pituitary (AP) or weight-matched intact controls were sampled under urethane anesthesia. Plasma growth hormone (GH) in sequential samples from each rat was measured by radioimmunoassay to determine the effect of exogenous thyrotropin-releasing hormo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-3-807
更新日期:1976-03-01 00:00:00
abstract::The regulation of cholecystokinin octapeptide (CCK-8) secretion was studied using a fetal cerebral cortical cell culture system. After 2-3 weeks in culture, the cells were utilized for short-term release experiments. CCK-8 was measured by RIA and its identity was confirmed by HPLC. Depolarization of the cells with K+ ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3125
更新日期:1991-12-01 00:00:00
abstract::The transcription factor Stat3 is activated through tyrosine phosphorylation by many cytokines and is a fundamental mediator of their signals. In the mammary gland, Stat3 activity increases sharply shortly after weaning, and involution is delayed in mice, that contain a mutant Stat3 lacking 33 amino acids including th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220224
更新日期:2002-09-01 00:00:00
abstract::Previous studies from our laboratory have demonstrated that epidermal growth factor (EGF), induces intracellular alkalinization in chicken granulosa cells by activating a sodium-dependent and amiloride-sensitive Na+/H+ antiporter. In the present investigation we have examined the possible involvement of protein kinase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-2957
更新日期:1991-12-01 00:00:00
abstract::Enhanced epinephrine secretion from adrenal chromaffin cells (ACCs) is an important homeostatic response to severe systemic inflammation during sepsis. Evidence suggests that increased activation of ACCs by preganglionic sympathetic neurons and direct alterations in ACC function contribute to this response. However, t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1623
更新日期:2014-01-01 00:00:00
abstract::Neuropeptide Y (NPY) is highly expressed in the hypothalamus, where it regulates feeding and energy homeostasis. Interestingly, NPY and its receptors are also expressed in peripheral tissues with roles in metabolism, including pancreatic islets. In islets, NPY is known to suppress insulin secretion acutely. In additio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1537
更新日期:2014-12-01 00:00:00
abstract::Homeostatic control of endocrine systems proceeds via feedforward (agonistic, stimulatory) and feedback (antagonistic, inhibitory) interactions mediated via implicit dose-response functions. However, neither the feedback/feedforward pathways nor the dose-response interfaces are directly observed in vivo. Thus, the goa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1461
更新日期:2011-12-01 00:00:00
abstract::In this study we used live-cell immunocytochemistry and confocal microscopy to study the release from a single vesicle in a simplified system called membrane lawns. The lawns were prepared by exposing differentiated pituitary prolactin (PRL)-secreting cells to a hypoosmotic shear stress. The density of the immunolabel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2022
更新日期:2013-03-01 00:00:00
abstract::The possibility that the responsiveness of plasma aldosterone concentration to angiotensin II alters with changes in sodium balance was investigated in male beagle dogs under conditions of controlled sodium and potassium intake. Angiotensin II was infused at four different rates (usually 3, 6, 12, and 24 ng/kg/min), e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-2-485
更新日期:1978-02-01 00:00:00
abstract::Evidence is presented that in vitamin D deficiency there are aberrations of estrogen metabolism in the laying hen. Five White Leghorn hens, 35 weeks of age, all laying soft shelled eggs as a result of complete withdrawal of supplemental vitamin D3 (D3) from the diet, were found to have a 3-fold higher molar ratio of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2170
更新日期:1984-12-01 00:00:00
abstract::GH is the major regulator of circulating IGF-I, which, in return, controls pituitary GH secretion by negative feedback. IGF-binding protein-1 (IGFBP-1) is believed to modify this feedback through its effects on free IGF-I. In the present study we investigated the potential influence of IGFBP-1 on GH secretion in the a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1742
更新日期:2004-09-01 00:00:00
abstract::The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1683
更新日期:1985-10-01 00:00:00
abstract::A subline of an x-ray-induced transplantable rat insulinoma has been studied in vivo and in vitro. Tumors grew rapidly after sc transplantation and were rich in insulin, but contained only small amounts of glucagon and somatostatin. Despite marked basal hyperinsulinemia, iv glucose administration caused a further incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2091
更新日期:1982-12-01 00:00:00
abstract::Magnocellular hypothalamic neurons in the rat supraoptic nucleus (SON) normally produce either vasopressin (VP) or oxytocin (OT). Here we demonstrate that many magnocellular neurons in the SON of lactating rats synthesize both hormones at the same time. We show the colocalization of the messenger (m) RNA that encodes ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1814
更新日期:1991-10-01 00:00:00
abstract::The effect of amino acid substitutions introduced to the third alpha-helix in bovine GH (bGH) was investigated. A GH analog (bGH-M8), in which three amino acids were substituted to form an idealized amphiphilic alpha-helix, possessed the same specific binding affinity as wild-type bGH to cell membranes prepared from 3...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547740
更新日期:1992-04-01 00:00:00
abstract::The quantities of estrone and estradiol were determined by radioimmunoassay in maternal and fetal plasma of the rhesus monkey from day 59 to 163 of gestation. A two way analysis of variance of data classified according to fetal sex and 3 pooled gestational ages for each hormone and for mother or fetus (4 analyses) rev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-425
更新日期:1975-08-01 00:00:00
abstract::Pancreatic islets express the superoxide-producing nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system, but its role remains unknown. To address this, we studied the mechanisms of impaired insulin secretion induced by diphenyleneiodium (DPI), an NADPH oxidase inhibitor. We investigated the effects of DP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0186
更新日期:2008-11-01 00:00:00
abstract::To address controversies of estrogen therapy, in vitro models of perimenopause and prevention vs. treatment modes of 17beta-estradiol (E(2)) exposure were developed and used to assess the neuroprotective efficacy of E(2) against beta-amyloid-1-42 (Abeta(1-42))-induced neurodegeneration in rat primary hippocampal neuro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0495
更新日期:2006-11-01 00:00:00
abstract::There is growing international interest in how environmental conditions experienced during development can shape adult phenotypes and the extent to which such induced changes are adaptive. One physiological system that links an individual to changes in environmental circumstances during development is the hypothalamic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1471
更新日期:2009-04-01 00:00:00
abstract::Signals derived from other pituitary cells can have a dramatic effect on PRL gene expression and secretion by mammotropes. However, the intracellular mechanisms by which these effects are manifested on the target cell remain unexplored. Inasmuch as calcium is a key modulator of both gene expression and hormone export ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6071
更新日期:1998-06-01 00:00:00
abstract::Sterol regulatory element binding protein 1c (SREBP1c) is a master regulator of lipogenic gene expression in liver and adipose tissue, where its expression is regulated by a heterodimer of nuclear receptor-type transcription factors retinoid X receptor-alpha (RXRalpha) and liver X receptor-alpha (LXRalpha). Despite th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1461
更新日期:2008-05-01 00:00:00
abstract::A recent report suggests that estrogen induction of hypothalamic progestin receptors is not a prerequisite for the facilitation by progesterone of female sexual behavior in rats. Progesterone was found to facilitate sexual behavior despite no apparent induction of progestin receptors by 1-(o-chlorophenyl) 1-(p-chlorop...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2052
更新日期:1984-12-01 00:00:00
abstract::The lateral hypothalamic area (LHA) is essential for motivated ingestive and locomotor behaviors that impact body weight, yet it remains unclear how the neurochemically defined subpopulations of LHA neurons contribute to energy balance. In particular, the role of the large population of LHA neurotensin (Nts) neurons h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00311
更新日期:2018-09-01 00:00:00
abstract::We have tested the hypothesis that basic fibroblast growth factor (bFGF) and transforming growth factor beta (TGF beta) regulate the proliferation of osteoblast-like cells. Cells which migrated from central bone explants of fetal calf calvaria expressed markers characteristic of the osteoblast phenotype, including ost...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-98
更新日期:1988-07-01 00:00:00
abstract::Anterior pituitary cells from adult male rats were cultured on a natural extracellular matrix (ECM). From the 5th day, spindle-shaped cells, fusing to form elongated fibers, were observed among the epithelial cells. These fibers later increased in size and number and were identified as striated muscle fibers, based on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-3002
更新日期:1988-06-01 00:00:00
abstract::We have used immunohistochemistry to define the cellular distribution of GH receptors in the gastrointestinal tract (GIT) and its derivatives. Immunohistochemistry was performed in the adult rat GIT with a panel of characterized monoclonal antibodies to the GH receptor. The most intense and heterogeneous immunoreactiv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-299
更新日期:1990-01-01 00:00:00
abstract::Aminoglutethimide, an inhibitor of pregnenolone synthesis, was used to study the relationship between oxidative phosphorylation and steroidogenesis in ovarian mitochondria. In vitro addition of aminoglutethimide to mitochondria prepared from porcine corpora lutea resulted in a decrease in oxygen uptake and an increase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-5-1528
更新日期:1980-05-01 00:00:00
abstract::The disappearance of 125-I-ACTH from the circulation of 4 pregnant and fetal sheep has been followed after a single injection to the ewe or fetus. The mean metabolic clearance rate for the fetus and the ewe was 55 and 34 ml/min/kg respectively, giving a half-life in each case of about 1 min. The higher fetal than mate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-1-231
更新日期:1975-01-01 00:00:00
abstract::The effects of insulin on lipoprotein lipase (LPL) messenger RNA (mRNA) were studied in mammary glands from virgin and late-pregnant rats. Virgin and pregnant rats (at 17 days of gestation) were subjected to a continuous infusion (35 ml/day) with 50% glucose for 72 h to produce a prolonged hyperinsulinemic-euglycemic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6565
更新日期:1999-03-01 00:00:00