Abstract:
:Aminoglutethimide, an inhibitor of pregnenolone synthesis, was used to study the relationship between oxidative phosphorylation and steroidogenesis in ovarian mitochondria. In vitro addition of aminoglutethimide to mitochondria prepared from porcine corpora lutea resulted in a decrease in oxygen uptake and an increase in the ADP to oxygen ratio, with maximal changes occurring at a concentration of 300 microM. Similar decreases in oxygen uptake were seen when aminoglutethimide was added to ovarian mitochondria from untreated and PMS gonadotropin-treated immature rats and intact and hypophysectomized mature rats. Also, aminoglutethimide increased the ADP to oxygen ratio in ovarian mitochondria from PMS gonadotropin-treated immature rats and intact mature rats. The addition of aminoglutethimide to luteal mitochondria inhibited their [4-14C]cholesterol conversion activity but increased their ATP synthesis rate by 25%. It is concluded that inhibition of ovarian mitochondrial steroidogenesis by aminoglutethimide is accompanied by both a decrease in oxygen consumption and an increase in ATP synthesis.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Dimino MJ,Chavis TR,Downing JRdoi
10.1210/endo-106-5-1528subject
Has Abstractpub_date
1980-05-01 00:00:00pages
1528-31issue
5eissn
0013-7227issn
1945-7170journal_volume
106pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The mechanism of mitogen-activated protein kinase (MAPK, ERK) stimulation by the GnRH analog [D-Trp6]GnRH (GnRH-a) was investigated in the gonadotroph-derived alphaT3-1 cell line. GnRH-a as well as the protein kinase C (PKC) activator 12-O-tetradecanoyl phorbol-13-acetate (TPA) stimulated a sustained response of MAPK ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.4.5057
更新日期:1997-04-01 00:00:00
abstract::Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. H...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1016
更新日期:2014-09-01 00:00:00
abstract::The ontogeny of expression of the CRF gene in the paraventricular hypothalamic nucleus and POMC gene in the pituitary was studied in rats using in situ hybridization histochemistry and Northern blotting techniques, respectively. CRF mRNA was first detected on day 17 of gestation (E17) in the paraventricular nucleus of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-60
更新日期:1989-01-01 00:00:00
abstract::Insulin is a potent regulator of Sp1 transcription factor. To examine if glucagon, which usually antagonizes insulin, regulates Sp1, we assessed the levels of Sp1 by Western blotting from H-411E cells exposed to glucagon with or without insulin. Glucagon alone (1.5 x 10(-9) to 1.5 x 10(-5) M) stimulated Sp1 accumulati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.4.8756
更新日期:2002-04-01 00:00:00
abstract::We investigated the effects of intraventricular (ivt) injection of bombesin on the TRH-induced increase in temperature in the midbrain reticular formation (mrf) of the rat brain under pentobarbital anesthesia. Ivt injection of TRH in a dose of 3 nmol significantly antagonized the decrease in brain temperature induced ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1864
更新日期:1986-10-01 00:00:00
abstract::We previously reported that cAMP inhibits autocrine IGF-I gene expression in rat C6 glioma cells. In this study we examined the influence of cAMP on IGF-binding protein gene expression in C6 cells. cAMP potently inhibited IGF-binding protein-3 mRNA and, to a lesser extent, IGF-binding protein-4 mRNA and transiently st...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8357
更新日期:2001-09-01 00:00:00
abstract::Evidence is presented that in vitamin D deficiency there are aberrations of estrogen metabolism in the laying hen. Five White Leghorn hens, 35 weeks of age, all laying soft shelled eggs as a result of complete withdrawal of supplemental vitamin D3 (D3) from the diet, were found to have a 3-fold higher molar ratio of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2170
更新日期:1984-12-01 00:00:00
abstract::The observation that angiotensin III is present in the circulation of the rat in amounts similar to those of angiotensin II has led to the notion that it may, in part, be formed by the action of converting enzyme on des-Asp-angiotensin I without the prior formation of angiotensin II. This possibility was studied in co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-406
更新日期:1981-02-01 00:00:00
abstract::Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. Gremlin, a BMP antagonist, is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. However, its effects in vivo are not known. To investigate the actions of gremlin on bone remodeling in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0766
更新日期:2005-02-01 00:00:00
abstract::It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0484
更新日期:2010-12-01 00:00:00
abstract::Insulin acts directly as a mitogen on isolated embryonic tissues but has been shown to be inactive at physiological concentrations on several fetal cell types. Since insulin availability is obligatory for optimal fetal growth, we have investigated its mitogenic actions on chondrocytes isolated from the epiphyseal grow...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2661
更新日期:1990-05-01 00:00:00
abstract::Regularly menstruating female rhesus monkeys were implanted sc with Silastic tubing packed with estrone. The implants were replaced every 6-8 months to maintain the serum estrone and estradiol concentrations constantly elevated 1.5-2.5-fold, i.e. in the midfollicular phase range, for 4 yr. Increasing the estrone and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2373
更新日期:1989-05-01 00:00:00
abstract::The majority of ovarian follicles undergo atresia through a mechanism involving apoptotic cell death. Although GnRH and its agonists have been shown to suppress ovarian growth and differentiation in hypophysectomized rats, studies on the induction of follicle atresia by GnRH are contradictory. In the present study, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275940
更新日期:1994-01-01 00:00:00
abstract::In rodents, soluble leptin receptor (SLR) may be generated by alternative splicing of ObR mRNA and/or as a cleavage product of ObR membrane-anchored receptors. In this study, we investigated the contribution of both processes on the generation of SLR in 3-, 8-, and 24-month-old Wistar rats fed ad libitum (AL) or under...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0220
更新日期:2005-11-01 00:00:00
abstract::To examine the relationship between the expression of insulin-like growth factor (IGF)-binding protein-2 (IGFBP-2) and cell growth in a cell type with a defined IGF/IGFBP system, an ovine IGFBP-2 complementary DNA was overexpressed in C6 glioma cells. C6 cells produce IGFBP-3, IGFBP-4, a negligible amount of IGFBP-2, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6498
更新日期:1999-02-01 00:00:00
abstract::The control of ACTH secretion by opiates seems to involve stimulatory and inhibitory pathways, since opiate agonists and antagonists are capable of releasing ACTH in conscious rats. To elucidate the role of different opiate receptors in the control of ACTH release, rats were treated with receptor-selective opiate agon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2688
更新日期:1985-06-01 00:00:00
abstract::L-700,653 is a potent nonpeptidyl GH secretagogue consisting of a benzolactam structure: (4'-[[3(R)-[[3-[(2(S),3-dihydroxypropyl) amino]3-methyl-1-oxobutyl]amino]2,3,4,5-tetrahydro-2-oxo-1H-1-benzaze pin- 1-yl]methyl][1,1'-biphenyl]2-carboxamide hydrochloride). When administered sc by a Medi-Jector device at 0, 0.003,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867560
更新日期:1995-03-01 00:00:00
abstract::The possibility of direct pituitary effects of sex steroids on gonadotropin gene expression and synthesis was studied in male rats. The animals were treated with a potent GnRH antagonist, Ac-D-pClPhe-D-pClPhe-D-Trp-Ser-Tyr-D-Arg-Leu-Arg-Pro-D-Ala-+ ++NH2CH3COOH (Org 30276; 0.5 mg/kg BW, sc, twice daily) for 10 days. G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-3204
更新日期:1990-06-01 00:00:00
abstract::One day of fasting suppresses pulsatile LH secretion in adult male rhesus monkeys. In the present study, we determined whether the hypothalamic-pituitary-adrenal (HPA) axis is activated by 1 day of fasting, and if there is any evidence that an increase in the activity of the HPA axis causes the fasting-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8389280
更新日期:1993-06-01 00:00:00
abstract::Molecular and cellular mechanisms involved in artificially induced ovarian differentiation were analyzed by exposing embryos of medaka (Oryzias latipes) to a potent nonsteroidal estrogen, diethylstilbestrol (DES). Embryos were exposed for short-exposure (SE) [from 0 to 8 d postfertilization (dpf)] and long-exposure (L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0812
更新日期:2011-02-01 00:00:00
abstract::In adult rats hysterectomized on day 8 of pseudopregnancy, the mean serum progesterone (P) level fell from 88 ng/ml on day 8 to 44 ng/ml on day 15 (n = 61). In response to a single sc injection of 0.5 ml of a specific antiserum to LH (LHAS) on day 10, the P level fell to less than 10 by day 15 in 29 of 33 rats; howeve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1750
更新日期:1986-10-01 00:00:00
abstract::The effect of collagenase dissociation of virgin mouse mammary glands on the level of mammary epithelial cytosolic estrogen receptors (ER) and progesterone receptors (PR) was assessed. After cell dissociation, ER was present in mammary epithelial cells at concentrations similar to those found in the whole gland. Howev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-105
更新日期:1985-01-01 00:00:00
abstract::Lactating rats exhibit stable individual differences in pup licking/grooming (LG) over the first week postpartum. Such naturally occurring variations in maternal behavior are associated with differences in estrogen-inducible oxytocin receptors in the medial preoptic area (MPOA) of the hypothalamus. We compared levels ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0564
更新日期:2003-11-01 00:00:00
abstract::Hereditary vitamin D-resistant rickets (HVDRR) is an autosomal recessive disease caused by mutations in the vitamin D receptor (VDR). We studied a young Saudi Arabian girl who exhibited the typical clinical features of HVDRR, but without alopecia. Analysis of her VDR gene revealed a homozygous T to C mutation in exon ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0080
更新日期:2004-11-01 00:00:00
abstract::The effect of conditioned vs. fresh culture medium on the dopaminergic inhibition of TSH and PRL secretion by primary cultures of male rat anterior pituitary cells has been studied. In the presence of conditioned medium (that had been in contact with the cells over the 3-day culture period) 10(-6) M dopamine (DA) inhi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1319
更新日期:1986-04-01 00:00:00
abstract::Stimulators of bone resorption, such as PTH, 1,25-dihydroxycholecalciferol [1,25-(OH)2D3], or prostaglandin E2 (PGE2), do not cause calcium release from cultured calvaria of the genetically determined osteopetrotic microphthalmic (mi/ mi) mouse, due to a defect in the function of osteoclasts. To investigate the capaci...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-945
更新日期:1983-03-01 00:00:00
abstract::Diets enriched in monounsaturated fatty acids (MUFA)s have been shown to benefit glycemic control. Furthermore, MUFAs specifically stimulate secretion of the antidiabetic hormone, Glucagon-like peptide-1 (GLP-1) in vitro. To determine whether the MUFA-induced benefit in glycemic tolerance in vivo is due to increased G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8034
更新日期:2001-03-01 00:00:00
abstract::Estrogen is implicated in many autoimmune diseases and is a robust immunomodulator. For example, it regulates interferon (IFN)-gamma, a cytokine believed to up-regulate inducible nitric oxide synthase (iNOS). A notable gap in the literature is a lack of information on the regulation of nitric oxide in immune tissues b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0829
更新日期:2006-02-01 00:00:00
abstract::Addition of somatostatin-14 (SRIF) inhibits corticotropin releasing factor (CRF) and forskolin-stimulated cyclic AMP formation and ACTH release from tumor cells of the mouse anterior pituitary (AtT-20/D16-16). After long-term pretreatment of these cells with SRIF, the ability of SRIF to inhibit CRF and forskolin-stimu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-2-811
更新日期:1983-08-01 00:00:00
abstract::The present study used perforated-patch electrophysiology and calcium imaging in GnRH transgenic mouse lines to determine the mechanisms underlying the potent excitatory effects of kisspeptin upon GnRH neurons in the acute brain slice preparation. Kisspeptin (100 nm) depolarized (6 +/- 1 mV) and/or evoked an 87 +/- 4%...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0321
更新日期:2008-09-01 00:00:00