Abstract:
:Insulin is a potent regulator of Sp1 transcription factor. To examine if glucagon, which usually antagonizes insulin, regulates Sp1, we assessed the levels of Sp1 by Western blotting from H-411E cells exposed to glucagon with or without insulin. Glucagon alone (1.5 x 10(-9) to 1.5 x 10(-5) M) stimulated Sp1 accumulation but inhibited insulin's (10,000 microU/ml) stimulatory effect on Sp1. We also assessed the effect of TNF-alpha, wortmannin, a PI3K inhibitor, and cAMP-dependent protein kinase inhibitor on Sp1 accumulation. While TNF-alpha (5 ng/ml) blocked insulin-stimulated Sp1, it failed to block stimulation of Sp1 by glucagon (1.5 x 10(-5) M). Similarly, wortmannin inhibited insulin- but not glucagon-stimulated Sp1, whereas protein kinase inhibitor had an opposite effect. Thus, insulin acts primarily via PI3K, and glucagon apparently stimulates through a cAMP-dependent pathway. Insulin increased the staining intensity of Sp1 seen exclusively in the nuclei of H-411E cells. Sp1 was demonstrable in both nucleus and cytoplasm after glucagon treatment. Finally, as judged by immunoblotting to specific antibody, insulin but not glucagon, stimulated O-glycosylation of Sp1. Thus, unique signaling mechanisms mediate the response of Sp1 to glucagon in the presence or absence of insulin.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Keembiyehetty CN,Candelaria RP,Majumdar G,Raghow R,Martinez-Hernandez A,Solomon SSdoi
10.1210/endo.143.4.8756subject
Has Abstractpub_date
2002-04-01 00:00:00pages
1512-20issue
4eissn
0013-7227issn
1945-7170journal_volume
143pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Small amounts of TRH are present in the thyroid, and TRH has been shown in several studies to have a moderate direct inhibitory effect on TSH-stimulated thyroid hormone secretion. In a recent publication, however, data were presented to demonstrate that very low doses of TRH given in short pulses induce immediate incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-385
更新日期:1988-02-01 00:00:00
abstract::Mesenchymal stroma/stem cells (MSCs) exist in adult tissues, such as adipose tissue and bone marrow, and differentiate into cells of multiple lineages. In previous studies, we found that MSCs differentiate into steroidogenic cells by forced expression of steroidogenic factor 1 (SF-1)/adrenal 4 binding protein (Ad4BP),...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa007
更新日期:2020-03-01 00:00:00
abstract::Up-regulation of maternal islet function is essential to accommodate the increased demand for insulin during pregnancy. Previously, we suggested that lactogenic activity regulates islet function during pregnancy. However, this hypothesis was based on the effect of homologous PRLs on islets, since the homologous placen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425500
更新日期:1993-02-01 00:00:00
abstract::In the rat a GH-binding protein (GHBP) exists that is derived from the GH receptor gene by an alternative messenger RNA splicing mechanism such that the transmembrane and intracellular domains of the GH receptor are replaced by a hydrophilic carboxy terminus. Previous immunohistochemical studies detailing the localiza...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1374020
更新日期:1992-05-01 00:00:00
abstract::The secretion of LH, PRL, and cortisol was investigated in 4 sexually mature female rhesus macaques with cardiac catheters protected by tethers. Based on endocrine parameters, all 4 of the animals ovulated within 2 months from the time they were tethered, and regular menstrual cycles of 24-34 days were observed. The c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-261
更新日期:1984-07-01 00:00:00
abstract::This study was undertaken to examine short photoperiod (SD; 8 h of light, 16 h of darkness)-induced alterations in reproductive endocrine and neuroendocrine parameters in the male white-footed mouse, Peromyscus leucopus. Exposure to SD for 8 weeks caused dramatic reductions in testis and seminal vesicle weights, decre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-2-1119
更新日期:1988-08-01 00:00:00
abstract::Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of anterior pituitary hormones. The little mutation in mice is analogous to autosomal recessive, isolated, partial deficiency...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-5-1672
更新日期:1982-05-01 00:00:00
abstract::Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0826
更新日期:2003-12-01 00:00:00
abstract::Amplification of insulin secretion by cAMP is mediated by protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). Using selective activators, we determined how each effector influences the cytosolic free Ca(2+) concentration ([Ca(2+)]c) and insulin secretion in mouse islets. Alone PKA activator ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1247
更新日期:2014-09-01 00:00:00
abstract::Deciduomal reactions in response to artificial stimuli were induced on the tube-ligated uterine horns of unilateral pregnant hamsters. Serum progesterone levels were determined, and the changes of estrogen and progesterone receptors (ER and PR, respectively) were simultaneously studied in the deciduoma. After stimulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-302
更新日期:1989-07-01 00:00:00
abstract::The classical model for the mechanism of action of steroids holds that unbound receptors for steroids reside exclusively in the cytoplasmic compartment and that they undergo translocation to the nucleus when bound to steroids in a process which is temperature sensitive. We have in the past proposed that unbound recept...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1533
更新日期:1981-04-01 00:00:00
abstract::We showed that insulin appears in the chick embryo before beta-cells are recognizable, as well as in the egg constituents even before fertilization. Acid-ethanol extracts of 2- to 8-day-old embryos were gel-filtered on Sephadex G-50. The peak of immunoreactive insulin chromatographed in a position corresponding to tha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1909
更新日期:1982-12-01 00:00:00
abstract::Adrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1080
更新日期:2005-03-01 00:00:00
abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013350
更新日期:1994-07-01 00:00:00
abstract::Studies on hormone-receptor interactions generally assume that the formation of a hormone-receptor complex is a reversible process. This assumption has been examined directly in three experiments using liver membrane receptor preparations from pregnant rats and ovine PRL (oPRL). In Exp 1, Receptors were preincubated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-1-402
更新日期:1980-01-01 00:00:00
abstract::Using a sensitive method for the simultaneous measurement of TSH subunit mRNAs, we have investigated their hormonal regulation in the pituitary glands of normal and hypothyroid rats. Oligodeoxyribonucleotides (probes) complementary to coding regions of rat alpha- and TSH beta-subunit mRNAs were synthesized. These prob...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-1272
更新日期:1985-09-01 00:00:00
abstract::Although estrogens are neuroprotective in young adult animal models of stroke, clinical trials demonstrate that estrogens increase the incidence and severity of stroke in aged women. We have previously shown that experimental stroke pathophysiology differs between young adult and aged rats. The aim of this study was t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1859
更新日期:2012-07-01 00:00:00
abstract::We report here 1) that GnRH and a potent GnRH agonist inhibit basal and LH stimulated ovarian androgen biosynthesis in vitro, 2) that the inhibitory effects of GnRH are dose-dependent and completely inhibited by concomitant treatment with a GnRH antagonist and 3) that the GnRH action is very rapid (t1/2 = 10 min) and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-661
更新日期:1981-08-01 00:00:00
abstract::To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LH beta mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the correspondi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-773
更新日期:1990-02-01 00:00:00
abstract::Recent evidence indicates that endogenous GnRH is required for maintenance of its own pituitary receptors (GnRH-R). We have measured GnRH-R in pituitaries of hypogonadotrophic hypogonadal (hpg) mice, in whom hypothalamic GnRH is deficient or absent. The GnRH-R concentration in hpg male mouse pituitaries was 10.6 +/- 1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-55
更新日期:1983-07-01 00:00:00
abstract::The response of purified rat testicular peritubular myoid cells (PMC) to platelet-derived growth factor (PDGF) was studied. Freshly isolated PMC were devoid of measurable amounts of PDGF-binding sites. However, after 1 day in culture in serum-free conditions, specific high affinity receptors were detected. The estimat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404630
更新日期:1993-10-01 00:00:00
abstract::To test the hypothesis that vitamin D-dependent calcium-binding protein (CaBP) and active calcium (Ca) transport in the small intestine of vitamin D-replete lactating rats are regulated by dietary Ca intake, pregnant rats were given a high Ca (1.6% Ca and 1.4% phosphorus) or low Ca (0.1% Ca and 0.4% phosphorus) diet s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-1-278
更新日期:1987-07-01 00:00:00
abstract::Prolonged exposure to elevated FFA levels has been shown to induce peripheral insulin resistance and to alter the beta-cell secretory response to glucose. To investigate the effects of FFAs on preproinsulin gene expression, we measured insulin release, cell content, and messenger RNA (mRNA) levels in rat islets after ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6953
更新日期:1999-09-01 00:00:00
abstract::SH2-containing inositol 5'-phosphatase 2 (SHIP2) is a 5'-lipid phosphatase hydrolyzing the phosphatidylinositol (PI) 3-kinase product PI(3,4,5)P(3) to PI(3,4)P(2) in the regulation of insulin signaling, and is shown to be increased in peripheral tissues of diabetic C57BL/KSJ-db/db mice. To clarify the impact of SHIP2 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0820
更新日期:2008-02-01 00:00:00
abstract::To find out whether the hippocampus is involved in central nervous system-mediated glucoregulation, we injected saline, neostigmine, dopamine, norepinephrine, bombesin, beta-endorphin, somatostatin, and prostaglandin F2 alpha into the dorsal hippocampus in anesthetized fed rats. After injection of dopamine, norepineph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2449
更新日期:1989-05-01 00:00:00
abstract::After 5 days of incubation with 10 nM tritiated dexamethasone, the amount of glucocorticoid displaceably-bound by the ACTH-secreting, glucocorticoid-responsive AtT-20 mouse pituitary tumor cell decreases by 70-80%. This decrease takes place in both the cytosolic and nuclear fractions. Although one might predict that t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-2-699
更新日期:1982-08-01 00:00:00
abstract::Magnocellular hypothalamic neurons in the rat supraoptic nucleus (SON) normally produce either vasopressin (VP) or oxytocin (OT). Here we demonstrate that many magnocellular neurons in the SON of lactating rats synthesize both hormones at the same time. We show the colocalization of the messenger (m) RNA that encodes ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1814
更新日期:1991-10-01 00:00:00
abstract::Early postnatal hyperalimentation has long-term implications for obesity and developing renal disease. Suppressor of cytokine signaling (SOCS) 3 inhibits phosphorylation of signal transducer and activator of transcription (STAT) 3 and ERK1/2 and thereby plays a pivotal role in mediating leptin resistance. In addition,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1670
更新日期:2012-03-01 00:00:00
abstract::Recent evidence has suggested that a circadian rhythm exists for plasma beta-endorphin-like immunoreactivity. The purpose of the present study was to examine the long term effects of surgical trauma on plasma beta-endorphin dynamics. Blood samples for RIA were obtained from female baboons every 4 h for three 48-h peri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1734
更新日期:1987-05-01 00:00:00
abstract::IGF-I and IGF-II play important roles in growth and development via interactions with cell-surface receptors; however, in nature, IGFs are sequestered by at least six soluble, high-affinity IGF-binding proteins (IGFBPs), namely IGFBPs 1-6. Herein, we demonstrate that the stromal cell-derived extracellular matrix-degra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0636
更新日期:2004-02-01 00:00:00