Abstract:
:After 5 days of incubation with 10 nM tritiated dexamethasone, the amount of glucocorticoid displaceably-bound by the ACTH-secreting, glucocorticoid-responsive AtT-20 mouse pituitary tumor cell decreases by 70-80%. This decrease takes place in both the cytosolic and nuclear fractions. Although one might predict that there should be a decrease in agonist efficacy associated with this loss of nuclear binding, there was not; long-term incubations with dexamethasone revealed that the AtT-20 cells showed no tendency to resume secreting ACTH. Hence, although there was a dramatic decrease in the amount of nuclear-bound steroid, there was not a resulting decrease in the steroid's biopotency. These results suggest that the agonist-induced decrease in receptor content is either a means of removing redundant nuclear receptors, or a new, previously unrecognized, step in the mechanism of glucocorticoid hormone action.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Svec F,Rudis Mdoi
10.1210/endo-111-2-699subject
Has Abstractpub_date
1982-08-01 00:00:00pages
699-702issue
2eissn
0013-7227issn
1945-7170journal_volume
111pub_type
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