Abstract:
:Groups of female ferrets born in the laboratory received sc Silastic capsules containing testosterone (T), 5 alpha-dihydrotestosterone (DHT), 17 beta-estradiol (E), or no steroid for 15 days beginning on the day of birth; an additional group of male ferrets received empty sc capsules neonatally. All ferrets were gonadectomized at 11 weeks of age and were subsequently tested for masculine and feminine sexual behaviors while being treated consecutively over an 8-month period with several different gonadal steroids. The ability to display masculine sexual behavior was studied in the absence of replacement hormones and during a counterbalanced sequence of treatments with Silastic capsules containing T, E, or DHT. The maximal amount of neck grip, mount, and pelvic thrusting behavior displayed, regardless of adult endocrine treatment, was significantly greater in control male and neonatally T-treated females than in females that had received no hormone, E, or DHT neonatally. Animals in all five groups displayed equivalent increments in sexual receptivity in response to daily sc injections of increasing dosages of estradiol benzoate. Polyacrylamide gel electrophoresis of plasma collected from newborn female ferrets revealed no binding of either [3H]E or [3H]T, whereas two binding peaks were found for [3H]DHT. After the administration of androgen in adulthood, equivalent clitoral growth and ossification occurred in females given either T or DHT neonatally. These results suggest that in ferrets, behavioral masculinization occurs in response to neonatal exposure to T itself and not to its major neural metabolites, E and DHT. They also show that behavioral defeminization fails to occur in ferrets even after neonatal exposure to pharmacological amounts of E, T, or DHT.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Baum MJ,Gallagher CA,Martin JT,Damassa DAdoi
10.1210/endo-111-3-773subject
Has Abstractpub_date
1982-09-01 00:00:00pages
773-80issue
3eissn
0013-7227issn
1945-7170journal_volume
111pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Hypothalamic proopiomelanocortin (POMC)-derived MSH peptides and the melanocortin receptor antagonist, agouti-related protein (AgRP), interact to regulate energy balance. Both POMC and AgRP neurons express estrogen receptors, but little is known about estrogen regulation of the melanocortin system in the primate. We h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0853
更新日期:2010-03-01 00:00:00
abstract::We previously reported that TNF-alpha-induced apoptosis of lactotropes is estrogen dependent and predominant at proestrus. Here we observed that TNF-alpha (50 ng/ml) failed to induce apoptosis of anterior pituitary cells from ovariectomized rats cultured in the presence of progesterone (10(-6) m). However, progesteron...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1276
更新日期:2005-02-01 00:00:00
abstract::Neuropeptide Y (NPY) is highly expressed in the hypothalamus, where it regulates feeding and energy homeostasis. Interestingly, NPY and its receptors are also expressed in peripheral tissues with roles in metabolism, including pancreatic islets. In islets, NPY is known to suppress insulin secretion acutely. In additio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1537
更新日期:2014-12-01 00:00:00
abstract::This report describes properties of highly purified cathepsin-B and an additional cysteine proteinase, designated cysteine proteinase I, obtained from human thyroids. Both enzymes are localized to lysosomes. The activity profile of cysteine proteinase I combined with its sensitivity to the active site inhibitor Z-Phe-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-2-1089
更新日期:1988-08-01 00:00:00
abstract::We have previously demonstrated in vitro that 1alpha,25-dihydroxyvitamin D3 (calcitriol) treatment increases p27 expression and decreases cell proliferation in cultured thyroid carcinoma cell lines. We hypothesized that in vivo treatment with calcitriol would have a beneficial effect on thyroid carcinoma growth and pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0785
更新日期:2004-12-01 00:00:00
abstract::In granulosa cells derived from medium-sized porcine follicles, certain androgens have been shown to inhibit FSH-stimulated progesterone synthesis. To determine the site at which this inhibition takes place, the effects of androgens on FSH- and (Bu)2cAMP-stimulated pregnenolone and progesterone syntheses were examined...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-6-1999
更新日期:1983-12-01 00:00:00
abstract::Steady state levels of plasma membrane receptors (measured in radioligand assays) result from processes that contribute to the production of receptors (synthesis, recycling, and unmasking) as well as those that contribute to the loss of receptors (degradation, internalization, and inactivation). Accordingly, we have p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2577
更新日期:1990-05-01 00:00:00
abstract::To assess the possible source of progesterone in the fetal fluids of pregnant sheep, we measured the changes in progesterone concentrations in amniotic fluid (AF) and allantoic fluid (ALF) during pregnancy and compared these values with those in the maternal and fetal circulations. In acute experiments, the concentrat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-2-481
更新日期:1982-02-01 00:00:00
abstract::To define the specific role of IGF-I receptor (IGF-IR) in adipogenic and thermogenic differentiation of brown adipocytes during late fetal life, we have established immortalized brown adipocyte cell lines from fetuses of IGF-IR-deficient mice (IGF-IR(-/-)) as well as from wild-type mice (IGF-IR(+/+)). IGF-IR(-/-) cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220828
更新日期:2003-02-01 00:00:00
abstract::Brown adipose tissue (BAT) thermogenesis is activated by the sympathetic nervous system. BAT responses to norepinephrine are blunted in hypothyroidism and are rapidly restored by thyroid hormone. We examined in rats the effects of thyroid hormone on BAT beta 1- and beta 2-adrenergic receptors (AR) expression and capac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628360
更新日期:1995-08-01 00:00:00
abstract::Pharmacological amounts of 25(s),26-dihydroxyvitamin D3 were administered to normal, vitamin D-replete rats in order to assess its pharmacological activity. Treatment with 25(s),26-dihydroxyvitamin D3 (20 micrograms/day for 1 week) caused a marked and significant fall in the circulating concentration of 1,25-dihydroxy...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1671
更新日期:1987-11-01 00:00:00
abstract::Somatostatin (SRIH) inhibits cell proliferation by interacting with five distinct SRIH receptor subtypes (SSTRs) activating several pathways in many tissues. We previously demonstrated that SRIH, by activating Src homology-2-containing protein, inhibits cell proliferation of the human medullary thyroid carcinoma cell ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1479
更新日期:2006-07-01 00:00:00
abstract::The effect of TSH stimulation on cellular (c)-myc mRNA content in cultured thyroid cells was examined by Northern blot analysis. Polyadenylated mRNA was prepared from quiescent FRTL-5 rat thyroid cells and from cells stimulated by TSH for 8 h. A major 2.1 kilobase (kb) transcript was enhanced 5.5-fold by TSH. (Bu)2cAM...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-2249
更新日期:1985-11-01 00:00:00
abstract::The effect of synthetic ovine CRF on ACTH secretion of dispersed, domestic fowl pituitary cells was investigated. Cells preincubated for 2 h, 16 h, or after 48-h culture were incubated briefly with CRF (up to 4 h). ACTH was bioassayed using isolated rat adrenocortical cells; ACTH-(1-24) served as the standard for expr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-143
更新日期:1986-01-01 00:00:00
abstract::The present studies aimed to identify mechanisms contributing to amylin/leptin synergy in reducing body weight and adiposity. We reasoned that if amylin/leptin harnessed complementary neuronal pathways, then in the leptin-sensitive state, amylin should augment leptin signaling/binding and that in the absence of endoge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0546
更新日期:2010-01-01 00:00:00
abstract::Pulsatile administration of glucagon to perifused rat hepatocytes stimulates hepatocyte glucose production (HGP) more effectively than continuous administration. Having established that this effect was due to delayed relaxation of glucagon-stimulated HGP (t1/2 for decay = 3.54 +/- 0.60 min) we wished to examine the me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-732
更新日期:1987-08-01 00:00:00
abstract::In a previous study we found that PTH stimulated bone resorption and release of insulin-like growth factor-I (IGF-I) and IGF-II from cultured neonatal mouse calvaria. Since IGF-I and IGF-II stimulate osteoblast proliferation and collagen synthesis, these results suggested that increased release of IGFs during resorpti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1511
更新日期:1991-03-01 00:00:00
abstract::Administration of the preferential mu-opioid receptor agonist, morphine, and selective K-opioid receptor agonists elicits activation of the hypothalamus-pituitary-adrenocortical axis, although the site or the molecular mechanisms for these effects have not been determined. The expression ofFos, the protein product of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.4.7407
更新日期:2000-04-01 00:00:00
abstract::Kisspeptins, products of the KiSS-1 gene with ability to bind G protein-coupled receptor 54 (GPR54), have been recently identified as major gatekeepers of reproductive function with ability to potently activate the GnRH/LH axis. Yet, despite the diversity of functional states of the female gonadotropic axis, pharmacol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1463
更新日期:2006-06-01 00:00:00
abstract::One day of fasting suppresses pulsatile LH secretion in adult male rhesus monkeys. In the present study, we determined whether the hypothalamic-pituitary-adrenal (HPA) axis is activated by 1 day of fasting, and if there is any evidence that an increase in the activity of the HPA axis causes the fasting-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8389280
更新日期:1993-06-01 00:00:00
abstract::Trophoblast hypoxia and injury, key components of placental dysfunction, are associated with fetal growth restriction and other complications of pregnancy. Accumulation of lipid droplets has been found in hypoxic nonplacental cells. Unique to pregnancy, lipid accumulation in the placenta might perturb lipid transport ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00752
更新日期:2018-12-01 00:00:00
abstract::Plasma immunoreactive PRL responses to indoleamines and their metabolites were studied in urethane-anesthetized rats. All drugs were injected into the lateral ventricle and blood samples were serially collected from a jugular vein. Serotonin and melatonin caused a significant increase in plasma PRL with peak values at...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-1-254
更新日期:1978-07-01 00:00:00
abstract::The proglucagon gene (glu) encodes the incretin hormone glucagon-like peptide-1 (GLP-1), produced in the intestinal endocrine L cells. We found previously that the bipartite transcription factor beta-catenin/T cell factor (cat/TCF), the major effector of the canonical Wnt signaling pathway, activates intestinal glu ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1142
更新日期:2008-05-01 00:00:00
abstract::Insulin-like growth factors I and II (IGF-I and -II) are polypeptides secreted by skeletal cells and are considered regulators of bone formation. IGF-I and -II were studied for their effects on collagen synthesis and degradation in cultures of intact fetal rat calvariae and on type I collagen transcript levels in oste...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-301
更新日期:1989-01-01 00:00:00
abstract::B-cell lymphoma 2 (BCL2) family kin (BFK) is a recently identified novel protein that is similar to proteins of the BCL2 family. In the present study, we discovered that the mouse Bfk transcript is expressed at the highest level in the epididymis. Two transcripts of 0.9 and 2.6 kb in size were identified, with alterna...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0018
更新日期:2007-07-01 00:00:00
abstract::Signals derived from other pituitary cells can have a dramatic effect on PRL gene expression and secretion by mammotropes. However, the intracellular mechanisms by which these effects are manifested on the target cell remain unexplored. Inasmuch as calcium is a key modulator of both gene expression and hormone export ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6071
更新日期:1998-06-01 00:00:00
abstract::Levels of plasma prolactin were estimated in ovariectomized, estrogen-treated rats following the systemic administration of several neural blocking and stimulating drugs. Phenoxybenzamine, an alpha-adrenergic blocker, at high doses, increased plasma prolactin. Phenotlamine, another alpha-adrenergic blocker, and propra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-313
更新日期:1975-02-01 00:00:00
abstract::The lactogen-dependent rat Nb2 lymphoma is a useful model to investigate PRL signaling pathways that lead to regulation of gene transcription. A primary mechanism coupled to PRL receptor (PRLR) activation in Nb2 cells involves phosphorylation by Jak-family tyrosine kinases of one or more signal transducers and activat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7215
更新日期:1999-12-01 00:00:00
abstract::A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) was purified by sequential fractionation of rat liver cytosol on Q-Sepharose, phenyl-Sepharose, red-Sepharose, and polyacrylamide gel electrophoresis under nondenaturing conditions. The CTBP had a sedimentation coefficient of 5.1S...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1701
更新日期:1991-10-01 00:00:00
abstract::The Pax6 transcription factor is crucial for pancreatic α-cells. Indeed, Pax6-deficient mouse models are characterized by markedly altered α-cell differentiation. Our objective was to investigate the role of Pax6 in glucagon secretion process. We used a Pax6-deficient model in rat primary enriched-α cells with specifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1425
更新日期:2012-09-01 00:00:00