Abstract:
:The effect of synthetic ovine CRF on ACTH secretion of dispersed, domestic fowl pituitary cells was investigated. Cells preincubated for 2 h, 16 h, or after 48-h culture were incubated briefly with CRF (up to 4 h). ACTH was bioassayed using isolated rat adrenocortical cells; ACTH-(1-24) served as the standard for expressing the data. Results with 16-h preincubated cells were as follows: CRF induced ACTH secretion in a concentration-dependent manner: ED50 and maximal stimulatory concentrations were 1.0 nM and 5.0 nM, respectively. CRF (10 nM) induced significant ACTH secretion within 10 min of incubation; maximal secretion (370% over basal value) was attained at 2 h. Dexamethasone (DEX) inhibited basal and CRF-induced ACTH secretion in a concentration-dependent manner; half-maximal inhibitory and maximal inhibitory concentrations were approximately 10 nM and 1 microM, respectively. In addition, DEX (10 microM) acutely (within 2 h) inhibited maximal CRF-induced ACTH secretion by 46%. 8-Bromo-cAMP (1 mM) also induced ACTH secretion, and DEX inhibited this secretion with a potency equivalent to that for CRF-induced ACTH secretion. In contrast to the effect of CRF, high concentrations (100 nM) of ovine LHRH, TRH, and synthetic human pancreatic GH-releasing factor (1-32) failed to induce significant ACTH secretion, thus suggesting that the effect of CRF was peptide specific. Domestic fowl pituitary cells cultured for 48 h before treatment also responded to CRF but not to any greater extent than that of 16-h preincubated cells. In contrast to 16-h preincubated cells or 48-h cultured cells, 2-h preincubated cells had high basal values of ACTH secretion that may have partially diminished or masked the actions of CRF. These data suggest that 1) CRF is a potent and specific stimulator of ACTH secretion by domestic fowl pituitary cells and 2) 16-h preincubated cells or 48-h cultured cells are amenable for other in vitro investigations on the regulation of avian ACTH secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Carsia RV,Weber H,Perez FM Jrdoi
10.1210/endo-118-1-143subject
Has Abstractpub_date
1986-01-01 00:00:00pages
143-8issue
1eissn
0013-7227issn
1945-7170journal_volume
118pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Cross talks between the vascular and immune system play a critical role in vascular diseases, in particular in atherosclerosis. The osteoclast-associated receptor (OSCAR) is a regulator of osteoclast differentiation and dendritic cell maturation. Whether OSCAR plays a role in vascular biology and has an impact on athe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1282
更新日期:2011-12-01 00:00:00
abstract::IL-6 abundance in amniotic fluid and uterine tissues increases in late gestation or with infection-associated preterm labor. A role in regulation of labor onset is suggested by observations that IL-6 increases expression of genes controlling prostaglandin synthesis and signaling in isolated uterine cells, but whether ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0063
更新日期:2010-08-01 00:00:00
abstract::Mitochondrial reactive oxygen species have been implicated in both diabetic complications and the progression of the underlying diabetic state. However, it is not clear whether mitochondria of diabetic origin are intrinsically altered to generate excess reactive oxygen species independent of the surrounding diabetic m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0404
更新日期:2009-01-01 00:00:00
abstract::Mouse kidney and urinary glycosphingolipids from developing C57BL/6J and adults of several other inbred strains were analyzed by high performance liquid chromatographic techniques. Glycosphingolipids from male and female C57BL/6J kidneys were similar until the fifth week of age. Galactosylceramide containing nonhydrox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-251
更新日期:1983-07-01 00:00:00
abstract::Diabetic cardiomyopathy is characterized by myocyte loss and myocardial fibrosis, leading to decreased elasticity and impaired contractile function. The study examines the downstream signaling whereby oxidative stress, induced by hyperglycemia, leads to myocardial fibrosis and impaired contractile function in the left...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0877
更新日期:2008-01-01 00:00:00
abstract::Although nicotinic cholinergic agonists have functional effects on PC 12 cells, radioligand-binding sites have not been detected. We, therefore, studied PC 12 cells incubated in the presence of nerve growth factor (NGF) and determined that specific [3H]nicotine-binding sites were induced approximately 2.5-fold in the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1733730
更新日期:1992-02-01 00:00:00
abstract::We investigated the effect of epidermal growth factor (EGF) on collagen and protein synthesis in clone MC3T30-E1, a cell line which retains osteoblast-like characteristics. EGF at concentrations of 2-50 ng/ml significantly the hydroxyproline content of the cell layer. These effects were completely abolished by the add...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1810
更新日期:1982-12-01 00:00:00
abstract::Despite a wealth of evidence for CRH mediating stress-induced suppression of the hypothalamic GnRH pulse generator, and hence reproductive dysfunction, the site and mechanism of action remains elusive. The locus coeruleus (LC), a prominent noradrenergic brain stem nucleus, is innervated by CRH neurons, mediates severa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1053
更新日期:2005-01-01 00:00:00
abstract::The induction of long-term responses in the uterus following estrogen treatment is discussed, with special reference to DNA synthesis. Immature female rats injected daily with estradiol-17beta or estriol (0.01 to 1 mug) or a combination of the two steroids for one, two or three consecutive days were sacrificed at inte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1501
更新日期:1976-12-01 00:00:00
abstract::The GnRH decapeptide controls reproductive function through its release from neuroendocrine terminals in the median eminence, a site where there is a convergence of numerous nerve terminals and glial cells. Previous work showed dynamic changes in the GnRH-glial-capillary network in the median eminence under different ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0679
更新日期:2009-12-01 00:00:00
abstract::The effects of pentobarbital (Pnt) treatment on the progesterone (P)-induced afternoon increase in the medial basal hypothalamic (MBH) LHRH and serum LH and FSH levels in ovariectomized estradiol benzoate-primed rats were studied. Pnt injection before P blocked the afternoon rise in serum gonadotropins but failed to a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1299
更新日期:1981-04-01 00:00:00
abstract::NADPH-cytochrome P-450 reductase (EC 1.6.2.4), the enzyme involved in progesterone 17-hydroxylation, was purified to apparent homogeneity by detergent solubilization of the microsomal fractions of liver and testis from untreated rats. The enzymes from these two tissues were then compared with regard to several paramet...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-811
更新日期:1986-08-01 00:00:00
abstract::Potassium deficiency produced by feeding mice a low potassium diet caused a marked decrease in plasma and testicular testosterone concentrations and a concomitant fall in the weight of seminal vesicles and in renal ornithine decarboxylase activity. All of these parameters were rapidly restored when potassium supply wa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.9.8756540
更新日期:1996-09-01 00:00:00
abstract::The low-density lipoprotein receptor (Ldlr) is a key molecule involved with lipid clearance. The Ldlr(-/-) mouse has been used extensively as a model for studying atherosclerosis. This study sought to characterize the energy balance phenotype of Ldlr(-/-) mice with respect to weight gain, body composition, energy expe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0496
更新日期:2010-11-01 00:00:00
abstract::Diabetes is associated with increased generation of cytokines and tissue inflammation, but it is unclear how increased cytokine synthesis is causally related to the development of diabetic complications. Here, we report that exposure to high (25 mm) glucose, but not iso-osmotic concentrations of mannitol or 3-methyl g...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0512
更新日期:2007-09-01 00:00:00
abstract::Angiotensin II (AngII) stimulates aldosterone biosynthesis in the zona glomerulosa of the adrenal cortex. AngII also triggers the MAPK pathways (ERK1/2 and p38). Because ERK1/2 phosphorylation is a transient process, phosphatases could play a crucial role in the acute steroidogenic response. Here we show that the dual...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0241
更新日期:2007-11-01 00:00:00
abstract::Ionic mechanisms play an important role in the regulation of hormone secretion. The GHRH-induced GH release by human GH-secreting cells is transmitted through protein kinase A (PKA), which activates nonselective cation current (NSCC) and induces membrane depolarization, intracellular Ca2+ increase, and GH secretion. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6731
更新日期:1999-05-01 00:00:00
abstract::We evaluated the capacity of estradiol (E(2)) to regulate PTHrP production, cell growth, tumor growth, and metastasis to the skeleton in breast cancer. In estrogen receptor (ER)-negative human breast cancer cells, MDA-MB-231, and cells transfected with full-length cDNA encoding ER (S-30), E(2) caused a marked decrease...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0062
更新日期:2005-07-01 00:00:00
abstract::We used differential display PCR on two GnRH producing cell lines to identify genes involved in GnRH gene expression and neuronal migration. RNA from Gn10 cells (derived from a tumor in the olfactory area when GnRH neurons are migrating and make low levels of GnRH) and from GT1-7 cells (derived from a tumor in forebra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.8.6221
更新日期:1998-08-01 00:00:00
abstract::Endometriosis-associated infertility has a multifactorial etiology. We tested the hypothesis that the endometrial response to the early embryonic signal, human chorionic gonadotropin (hCG), alters over time in a nonhuman primate model of endometriosis. Animals with experimental or spontaneous endometriosis were treate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0275
更新日期:2010-10-01 00:00:00
abstract::The present study assesses the relationship between G-proteins and protein kinase-C (PKC) in the gonadotrope. Cells were depleted of PKC with 1 microM phorbol 12-myristate 13-acetate for 12 h, followed by medium 199-BSA for 6 h before treatment with vehicle, pertussis toxin (PTX), cholera toxin (CTX), or (Bu)2cAMP (dB...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243300
更新日期:1993-12-01 00:00:00
abstract::LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867570
更新日期:1995-03-01 00:00:00
abstract::Glucagon-like peptide 1 (7-36)amide (GLP-1) is an insulinotropic intestinal peptide hormone with a potential role as antidiabetogenic therapeutic agent. It mediates a potentiation of glucose-induced insulin secretion, by activation of adenylate cyclase and subsequent elevation of cytosolic free calcium, [Ca2+]cyt. We ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6947
更新日期:1999-09-01 00:00:00
abstract::The neuroendocrine hormones ACTH and corticotropin- releasing factor (CRF), which are involved in the stress response, have acute effects on arterial pressure. New evidence indicates that urocortin (UCN), the putative agonist for the CRF type 2 receptor, has selective cardiovascular actions. The responses to long-term...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0432
更新日期:2004-12-01 00:00:00
abstract::The cells from the 2B8 clonal strain of pituitary cells which previously have been reported to produce only PRL were incubated with varying amounts of ovine PRL (oPRL) ranging from 0.01--1000 ng/ml. After 1 h, significantly less PRL was measured in culture media from cells incubated with all doses of oPRL employed. Th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-1-97
更新日期:1979-01-01 00:00:00
abstract::The peptides angiotensin II (ANGII) and atrial natriuretic factor (ANF) regulate blood pressure and salt and water balance by producing antagonistic physiological effects in a variety of tissues. We used in vitro autoradiography with [125I] ANGII and [125I]ANF to compare receptor regulation for both peptides in variou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2799
更新日期:1989-06-01 00:00:00
abstract::The present studies were performed to determine whether the major biologically active metabolite of vitamin D3, 1,25-dihydroxycholecalciferol [1,25(OH)2D3], could influence the activities of rat colonic particulate guanylate cyclase and adenylate cyclase. To address these issues, colonocytes were harvested from Spragu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8104780
更新日期:1993-11-01 00:00:00
abstract::The contribution of the insulin receptor beta-subunit acidic domain, amino acids 1262-1291, to receptor function was analyzed. A mutant insulin receptor complementary DNA lacking this domain was created. Rat-1 fibroblasts were stably transfected with plasmids containing the mutant insulin receptor complementary DNA an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.8396020
更新日期:1993-09-01 00:00:00
abstract::Certain metabolic properties of hormonally responsive osteogenic sarcoma cells derived from a transplantable rat tumor have been compared with those of related normal rat bone cells. All studies were carried out on cells grown in monolayer culture. Normal rat bone cells derived by repeated collagenase/trypsin digestio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-213
更新日期:1981-01-01 00:00:00
abstract::Small amounts of TRH are present in the thyroid, and TRH has been shown in several studies to have a moderate direct inhibitory effect on TSH-stimulated thyroid hormone secretion. In a recent publication, however, data were presented to demonstrate that very low doses of TRH given in short pulses induce immediate incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-385
更新日期:1988-02-01 00:00:00