Abstract:
:Despite a wealth of evidence for CRH mediating stress-induced suppression of the hypothalamic GnRH pulse generator, and hence reproductive dysfunction, the site and mechanism of action remains elusive. The locus coeruleus (LC), a prominent noradrenergic brain stem nucleus, is innervated by CRH neurons, mediates several behavioral stress responses, and is implicated in the control of pulsatile LH secretion. The aim of this study was to test the hypothesis that LC CRH has a critical role in mediating stress-induced suppression of pulsatile LH secretion in the rat. Ovariectomized rats with 17beta-estradiol or oil-filled s.c. capsules were implanted with bilateral LC and i.v. cannulae. Central administration of CRH (10 ng to 1 microg) resulted in a dose-dependent suppression of LH pulses, which was reversed by a CRH receptor antagonist (alpha-helical CRF(9-41), 1 microg). The induction of c-fos expression in glutamic acid decarboxylase67 immunostained neurons in the preoptic area suggests activation of the secretion of gamma-aminobutyric acid in response to intracoerulear administration of CRH; 17beta-estradiol further increased the percentage of glutamic acid decarboxylase67-positive neurons that expressed fos and augmented suppression of LH pulses. Furthermore, intracoerulear administration of alpha-helical CRF(9-41) completely blocked restraint stress-induced suppression of LH pulses, without affecting the inhibitory response to hypoglycemia. These results suggest that CRH innervation of the LC may play a pivotal, but differential, role in the normal physiological response of stress-induced suppression of the GnRH pulse generator and hence the reproductive system.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Mitchell JC,Li XF,Breen L,Thalabard JC,O'Byrne KTdoi
10.1210/en.2004-1053subject
Has Abstractpub_date
2005-01-01 00:00:00pages
323-31issue
1eissn
0013-7227issn
1945-7170pii
en.2004-1053journal_volume
146pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Prolactin (PRL) is a vertebrate hormone with diverse actions in osmoregulation, metabolism, reproduction, and in growth and development. Osmoregulation is fundamental to maintaining the functional structure of the macromolecules that conduct the business of life. In teleost fish, PRL plays a critical role in osmoregul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1969
更新日期:2016-09-01 00:00:00
abstract::We previously reported that human fibroblasts secrete a protease into their conditioned medium that cleaves insulin-like growth factor binding protein-5 (IGFBP-5) into non-IGF-I binding fragments. Because the protease activity in the fibroblast medium has characteristics of both serine and metalloproteases, the activi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940380
更新日期:1996-12-01 00:00:00
abstract::Daily treatment with 1 mg of the GnRH antagonist [Ac-delta 3-Pro1,pC1-D-Phe2,D-Trp3,6,N alpha MeLeu7]-GnRH abolished mating behavior and disrupted spermatogenesis in male rats within 2 weeks. This was accompanied by a sharp decrease in plasma testosterone (T) levels as well as sexual organ weights while body weight re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1998
更新日期:1981-05-01 00:00:00
abstract::The maternal plasma concentrations of GH and PRL increase dramatically upon the initiation of lactation in the rat. In light of the fact that these two hormones have evolved from one common precursor, we sought to determine if the neuroendocrine mechanisms regulating their concomitant increase during lactation are com...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-464
更新日期:1989-01-01 00:00:00
abstract::Human pituitary prolactin (hPRL) contains a single major component. Upon iodination by a lactoperoxidase procedure approximately 50% of this component remains ulaltered in molecular size and net charge by the criteria of quantitative polyacrylamide gel electrophoresis (PAGE). Aminiotic fluid hPRL contains two componen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-406
更新日期:1975-08-01 00:00:00
abstract::The insulin-like growth factors (IGFs) stimulate the proliferation of human breast cancer cells, including the estrogen-dependent cell line MCF-7. These cells secrete regulatory IGF-binding proteins (IGFBPs) which may enhance or attenuate IGF-stimulated cell proliferation. In this study, we have used RIA to quantify t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7532580
更新日期:1995-03-01 00:00:00
abstract::The effects of peripheral glucagon like peptide-1 receptor (GLP-1R) stimulation on feeding, gastric emptying, and energetic responses involve vagal transmission and central nervous system processing. Despite a lack of studies aimed at determining which central nervous system regions are critical for the GLP-1R respons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1743
更新日期:2008-08-01 00:00:00
abstract::Proinflammatory cytokines have been shown to influence the expression and function of the glucocorticoid receptor (GR). Specifically, several studies have found that cytokines induce a decrease in GR function, as evidenced by reduced sensitivity to glucocorticoid effects on functional end points. To investigate the po...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6986
更新日期:1999-09-01 00:00:00
abstract::Previous studies have shown that estradiol indirectly stimulated the proliferation of oviduct epithelial cells in the quail. The present study was undertaken to investigate the effects of estradiol and other steroid hormones on the cAMP concentration. The ability of forskolin, a specific activator of the catalytic sub...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-1-158
更新日期:1988-01-01 00:00:00
abstract::Addition of calcitonin gene-related peptide (CGRP), 1 X 10(-7) M, to cultured neonatal rat heart cells resulted in rapid increases in beating rate and cellular concentrations of cAMP. Calcitonin (1 X 10(-7) M), in contrast, had no significant effect on heart cell beating rate or cAMP content. CGRP-stimulated increases...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-106
更新日期:1988-07-01 00:00:00
abstract::In vitro addition of rat insulin (200, 400 or 800 muU/ml) to collagenase-isolated pancreatic islets of adult rats diminished glucose (3 mg/ml)-induced insulin release which was correlated with a decrease of the ratio of total NADPH/NADP and inhibition of glucose oxidation via the pentose phosphate shunt (PPS). NADH an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1469
更新日期:1976-12-01 00:00:00
abstract::Estradiol (E(2)) rapidly and strongly induces vascular endothelial growth factor (VEGF) transcription in uterine endometrial epithelial cells in vivo. We have shown that this is mediated by both the estrogen receptor-alpha and hypoxia-inducible factor (HIF)-1alpha. By contrast, E(2) induces little or no VEGF expressio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0884
更新日期:2009-12-01 00:00:00
abstract::We tested further our postulate that enhanced de novo synthesis of phosphatidic acid is responsible for ACTH- and cAMP-induced increases in adrenal phospholipids in the phosphatidate polyphosphoinositide pathway. During incubation of adrenal sections or cells in vitro, ACTH and cAMP increased the concentrations of and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-1895
更新日期:1981-12-01 00:00:00
abstract::Antisera were raised against synthetic replicates of the carboxyl-terminal (C-terminal) fragment of the precursor to human GH-releasing factor (GRF) (pre-proGRF) whose structure was predicted from the complementary DNA cloned from a pancreatic tumor. These antisera were used along with antisera to human GRF itself to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-156
更新日期:1986-01-01 00:00:00
abstract::It has previously been established that neuropeptide-Y (NPY) is a potent inhibitor of alpha MSH release from the pars intermedia of the amphibian Xenopus laevis. The location of binding sites for NPY in the pars intermedia of the pituitary has now been studied with light microscopic autoradiography, using a dispersed ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-735
更新日期:1991-02-01 00:00:00
abstract::The intermediate filament protein nestin was originally found to be expressed in neuronal progenitor cells, but recent studies have shown that other cell types, including endocrine and vascular endothelial cells, express nestin. In the present study, we examined the expression and localization of nestin in the ovaries...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0774
更新日期:2008-01-01 00:00:00
abstract::Mitochondrial reactive oxygen species have been implicated in both diabetic complications and the progression of the underlying diabetic state. However, it is not clear whether mitochondria of diabetic origin are intrinsically altered to generate excess reactive oxygen species independent of the surrounding diabetic m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0404
更新日期:2009-01-01 00:00:00
abstract::Oxytocin (OT) is released by the posterior pituitary during male orgasm and is supposed to participate in the ejaculatory process. We now report evidence demonstrating the presence of an OT receptor gene (real-time RT-PCR and Northern blot) and protein (immunohistochemistry, Western blot, and binding studies) expressi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0962
更新日期:2004-04-01 00:00:00
abstract::The developmental patterns of thyroid-stimulating hormone (TSH) was examined in female Long-Evans rats by radioimmunoassay of pituitary and plasma from 5 through 80 days of age. The effects of hypothyroidism on normal TSH levels were examined in animals radiothyroidectomized at birth. Control animals showed a peak in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-1-237
更新日期:1975-07-01 00:00:00
abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0473
更新日期:2006-09-01 00:00:00
abstract::Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0334
更新日期:2006-09-01 00:00:00
abstract::We previously examined the segregation patterns of two polymorphic restriction enzyme sites for EcoRI and HindIII in the 3'-flanking region of human CG-alpha gene in placenta and choriocarcinoma. We observed that a unique combination of restriction sites predominates in choriocarcinoma while this pattern was rare in p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-2124
更新日期:1986-11-01 00:00:00
abstract::The GH/IGF-I axis has both pre- and postpubertal metabolic effects. However, the differential effects of GH and/or IGF-I on animal physiology or the plasma proteome are still being unraveled. In this report, we analyzed several physiological effects along with the plasma proteome after treatment of mice with recombina...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1217
更新日期:2011-10-01 00:00:00
abstract::Neuropeptide Y (NPY) is a peptide found in a variety of hypothalamic loci which is frequently colocalized with catecholamines. It is also secreted into hypophyseal portal vessels. The injection of NPY into the third ventricle (3V) lowered plasma GH levels in conscious, freely moving male rats. To determine the physiol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2296
更新日期:1990-05-01 00:00:00
abstract::Complementary DNAs encoding two forms of cocaine- and amphetamine-regulated transcript (CART) peptide precursors were identified from goldfish brain and named CART I and CART II. Each cDNA contains a signal peptide sequence, the putative CART-like peptide, and a carboxy-terminal extension peptide. Form I encodes a 117...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8519
更新日期:2001-12-01 00:00:00
abstract::Trans-10, cis-12 conjugated linoleic acid (10,12 CLA) is a dietary fatty acid that promotes weight loss and disproportionate fat loss. Obese mice fed a high-fat, high-sucrose (HFHS) diet containing 10,12 CLA are resistant to weight gain and contain markedly reduced subcutaneous fat and adiponectin, with a concurrent l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00213
更新日期:2017-09-01 00:00:00
abstract::Perifused anterior hemipituitaries from one male and 4 female monkeys released GH and PRL in a pulsatile pattern, with mean +/- SE interpulse intervals of 8.2 +/- 0.4 and 8.5 +/- 0.3 min, as determined by a cycle detection computer algorithm. Mean hormone concentrations in the perifusate fractions collected at 2-min i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-1
更新日期:1985-01-01 00:00:00
abstract::To investigate trafficking in neuroendocrine cells, green fluorescent protein (GFP) tags were fused to various portions of the preproneuropeptide Y (NPY) precursor. Two neuroendocrine cell lines, AtT-20 corticotrope tumor cells and PC-12 pheochromocytoma cells, along with primary anterior pituitary cells, were examine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.7929
更新日期:2001-02-01 00:00:00
abstract::The human estrogen receptor alpha (ERalpha) and the recently identified ERbeta share a high degree of amino acid homology; however, there are significant differences in regions of these receptors that would be expected to influence transcriptional activity. Consequently, we compared the mechanism(s) by which these rec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7179
更新日期:1999-12-01 00:00:00
abstract::We previously reported a cooperative effect between insulin-like growth factor I (IGF-I) and 1,25-dihydroxy-vitamin D3 [1,25-(OH)2D3] in murine clonal osteoblastic cells, MCT3T3-E1. In the present study, the possible mechanism of interaction between these hormones was investigated. The effect of IGF-I on 1,25-(OH)2D3 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-4-2088
更新日期:1990-04-01 00:00:00