Bone re/modeling is more dynamic in the endothelial nitric oxide synthase(-/-) mouse.


:Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO synthase (eNOS) in bone cells and bone progenitor cells at regular intervals up to 10 wk after acute estrogen deprivation. Ovariectomy led to an anticipated initial decline in bone cell eNOS production, but surprisingly, 17 d after ovariectomy, eNOS expression by bone and marrow stromal cells dramatically rebounded and was maintained at high levels for at least 10 wk after surgery. We examined the long-term consequences of eNOS in the process of ovariectomy-induced bone loss by prospectively analyzing bone mineral density in wild-type and eNOS(-/-) mice for 10 wk after ovariectomy. Ovariectomized eNOS(-/-) mice were observed to undergo an exaggerated state of estrogen-deficiency-induced bone remodeling compared with wild-type controls, suggesting that eNOS may act to mitigate this process. Furthermore, we found that whereas bone formation in estrogen-replete wild-type mice slowed between 14 and 20 wk of age, eNOS knockout mice continued to accrue basal bone mass at a high rate and showed no sign of entering a remodeling stage. Our data suggest that eNOS may play an important role in limiting ovariectomy-induced bone remodeling as well as regulating the transition from basal modeling to remodeling.






Grassi F,Fan X,Rahnert J,Weitzmann MN,Pacifici R,Nanes MS,Rubin J




Has Abstract


2006-09-01 00:00:00














  • A new hormone-response hydrolase activity in the mouse uterus.

    abstract::A new hydrolase activity has been identified by its capability of cleaving t-boc-Ala-Ala-Pro-Ala-AMC, the released 7-amino-4-methylcoumarin (AMC) being quantified fluorometrically. The activity in the 28,000 X g supernatant fraction of homogenates of weanling mouse uterus was about one fifth that of the adult mouse. T...


    pub_type: 杂志文章


    authors: Katz J,Finlay TH,Tom C,Levitz M

    更新日期:1980-12-01 00:00:00

  • Minireview: The sodium-iodide symporter NIS and pendrin in iodide homeostasis of the thyroid.

    abstract::Thyroid hormones are essential for normal development and metabolism. Thyroid hormone biosynthesis requires iodide uptake into the thyrocytes and efflux into the follicular lumen, where it is organified on selected tyrosyls of thyroglobulin. Uptake of iodide into the thyrocytes is mediated by an intrinsic membrane gly...


    pub_type: 杂志文章,评审


    authors: Bizhanova A,Kopp P

    更新日期:2009-03-01 00:00:00

  • Evidence for a specific uptake and retention mechanism for 25-hydroxyvitamin D (25OHD) in skeletal muscle cells.

    abstract::Little is known about the mechanism for the prolonged residence time of 25-hydroxyvitamin D (25OHD) in blood. Several lines of evidence led us to propose that skeletal muscle could function as the site of an extravascular pool of 25OHD. In vitro studies investigated the capacity of differentiated C2 murine muscle cell...


    pub_type: 杂志文章


    authors: Abboud M,Puglisi DA,Davies BN,Rybchyn M,Whitehead NP,Brock KE,Cole L,Gordon-Thomson C,Fraser DR,Mason RS

    更新日期:2013-09-01 00:00:00

  • Regulation and actions of Smad7 in the modulation of activin, inhibin, and transforming growth factor-beta signaling in anterior pituitary cells.

    abstract::Activins and transforming growth factor-beta (TGF beta) are crucial autocrine, paracrine, and endocrine modulators of anterior pituitary function. Activins regulate most pituitary cells and lactotropes are targets of TGF beta. Smad2 and Smad3 are two cellular mediators of activin/TGF beta signaling, whereas Smad7 is a...


    pub_type: 杂志文章


    authors: Bilezikjian LM,Corrigan AZ,Blount AL,Chen Y,Vale WW

    更新日期:2001-03-01 00:00:00

  • Activation of antimetastatic Nm23-H1 gene expression by estrogen and its alpha-receptor.

    abstract::Metastasis of various malignant cells is inversely related to the abundance of the Nm23-H1 protein. The role of estrogens in tumor metastasis has now been investigated by examining the effect of E2 on the expression of the Nm23-H1 gene. Three human breast carcinoma cell lines, in which endogenous ERalpha is expressed ...


    pub_type: 杂志文章


    authors: Lin KH,Wang WJ,Wu YH,Cheng SY

    更新日期:2002-02-01 00:00:00

  • Immunological properties of the beta-subunit of human chorionic gonadotropin. I. Effect of chemical and enzymatic modifications.

    abstract::The beta-subunit of hCG (hCG beta) displays immunological cross-reactivity with human LH (hLH). A detailed study was undertaken to investigate the effect of chemical and enzymatic modifications on the immunological behavior of hCG beta, particularly on its cross-reactivity with hLH, with a view to obtaining a highly h...


    pub_type: 杂志文章


    authors: Ghai RD,Mise T,Pandian MR,Bahl OP

    更新日期:1980-11-01 00:00:00

  • Therapeutic effects of dehydroepiandrosterone metabolites in diabetes mutant mice (C57BL/KsJ-db/db).

    abstract::Dehydroepiandrosterone (DHEA) fed at 0.4% in the diet is known to exert strong antihyperglycemic effects in C57BL/KsJ genetically diabetic (db/db) mice. Three of the major metabolic products of DHEA; DHEA sulfate, alpha-hydroxyetiocholanolone (alpha-ET), and beta-hydroxyetiocholanolone (beta-ET) when fed at 0.1% in th...


    pub_type: 杂志文章


    authors: Coleman DL,Leiter EH,Applezweig N

    更新日期:1984-07-01 00:00:00

  • Genetic Disruption of 21-Hydroxylase in Zebrafish Causes Interrenal Hyperplasia.

    abstract::Congenital adrenal hyperplasia is a group of common inherited disorders leading to glucocorticoid deficiency. Most cases are caused by 21-hydroxylase deficiency (21OHD). The systemic consequences of imbalanced steroid hormone biosynthesis due to severe 21OHD remains poorly understood. Therefore, we developed a zebrafi...


    pub_type: 杂志文章


    authors: Eachus H,Zaucker A,Oakes JA,Griffin A,Weger M,Güran T,Taylor A,Harris A,Greenfield A,Quanson JL,Storbeck KH,Cunliffe VT,Müller F,Krone N

    更新日期:2017-12-01 00:00:00

  • Inactivation of early glucocorticoid feedback by corticotropin-releasing factor in vitro.

    abstract::We have investigated the interaction between hypothalamic ACTH secretagogues and adrenocortical glucocorticoids in rat anterior pituitary tissue using an in vitro perifusion system. Repeated 5 min pulses of 41-residue CRF (CRF-41) or arginine vasopressin (AVP) were applied at 1 h intervals for up to 7 h. Administratio...


    pub_type: 杂志文章


    authors: Shipston MJ,Antoni FA

    更新日期:1992-04-01 00:00:00

  • Biphasic effects of transforming growth factor-beta on the production of osteoclast-like cells in mouse bone marrow cultures: the role of prostaglandins in the generation of these cells.

    abstract::Osteoclast-like multinucleated giant cells are induced in bone marrow cultures by 1,25-dihydroxyvitamin D3 and other agents. These cells resemble osteoclasts in their morphology, their ability to resorb bone, and the possession of calcitonin receptors. We report here a biphasic effect of transforming growth factor-bet...


    pub_type: 杂志文章


    authors: Shinar DM,Rodan GA

    更新日期:1990-06-01 00:00:00

  • Tissue-specific regulation of alpha 1B, beta 1, and beta 2-adrenergic receptor mRNAs by thyroid state in the rat.

    abstract::Steady state levels of the mRNAs for alpha 1B, beta 1- and beta 2-adrenergic receptors (alpha 1BAR, beta 1AR, beta 2AR) were quantified by DNA excess solution hybridization assays in the heart, lungs, and liver of rats. Tissues for RNA extraction were obtained from euthyroid and thyroidectomized rats and from thyroide...


    pub_type: 杂志文章


    authors: Lazar-Wesley E,Hadcock JR,Malbon CC,Kunos G,Ishac EJ

    更新日期:1991-08-01 00:00:00

  • Parathyroid hormone messenger ribonucleic acid in the rat hypothalamus.

    abstract::Polyadenylated RNA, extracted from rat hypothalami, cross-hybridized with a RNA probe complementary in sequence to rat PTH (rPTH) messenger RNA (mRNA). Amplification of complementary DNA (cDNA) by the polymerase chain reaction also demonstrated the presence of rPTH mRNA in the rat hypothalamus and parathyroid gland. r...


    pub_type: 杂志文章


    authors: Fraser RA,Kronenberg HM,Pang PK,Harvey S

    更新日期:1990-11-01 00:00:00

  • Parathyroid hormone-like effects of rainbow trout Stannius products on bone resorption of embryonic mouse calvaria in vitro.

    abstract::Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-depe...


    pub_type: 杂志文章


    authors: Lafeber FP,Schaefer HI,Herrmann-Erlee MP,Wendelaar Bonga SE

    更新日期:1986-11-01 00:00:00

  • Differential effects of growth hormone versus insulin-like growth factor-I on the mouse plasma proteome.

    abstract::The GH/IGF-I axis has both pre- and postpubertal metabolic effects. However, the differential effects of GH and/or IGF-I on animal physiology or the plasma proteome are still being unraveled. In this report, we analyzed several physiological effects along with the plasma proteome after treatment of mice with recombina...


    pub_type: 杂志文章


    authors: Ding J,List EO,Bower BD,Kopchick JJ

    更新日期:2011-10-01 00:00:00

  • Inhibition of adenylate cyclase by angiotensin II in rat renal cortex.

    abstract::Adenylate cyclase of rat renal cortex was inhibited by angiotensin II (AII). Inhibition required Na+ (100-200 mM) and GTP (10(-8)-10(-4) M) and was opposed by the receptor antagonist [1-sarcosine, 8-isoleucine]AII. The EC50 value (+/- SE)for inhibition by AII was 3.7 +/- 1.2 nM, and the maximum inhibition (+/- SE) was...


    pub_type: 杂志文章


    authors: Woodcock EA,Johnston CI

    更新日期:1982-11-01 00:00:00

  • An autocrine progesterone positive feedback loop mediates oxytocin upregulation in bovine granulosa cells during luteinization.

    abstract::During luteinization of bovine granulosa cells in vitro in the presence of insulin, or insulin plus forskolin, there is a massive upregulation not only of progesterone production, but also of the gene for the peptide hormone oxytocin, with secretion of the peptide into the medium. By using the progesterone receptor an...


    pub_type: 杂志文章


    authors: Lioutas C,Einspanier A,Kascheike B,Walther N,Ivell R

    更新日期:1997-11-01 00:00:00

  • Pituitary homeobox 1 (Ptx1) is differentially expressed during pituitary development.

    abstract::Pituitary homeobox 1 (Ptx1) is a homeodomain-containing transcription factor acting on transcription of all pituitary hormone genes. Its expression is first detected in the stomodeal ectoderm and is maintained in all derivatives of this structure, including Rathke's pouch. We now show that Ptx1 is expressed in all pit...


    pub_type: 杂志文章


    authors: Lanctôt C,Gauthier Y,Drouin J

    更新日期:1999-03-01 00:00:00

  • Insulin radioreceptor assay on murine splenic leukocytes and peripheral erythrocytes.

    abstract::Insulin radioreceptor assays were developed using splenic leukocytes and peripheral erythrocytes from individual mice. Splenic leukocytes were prepared using an NH4Cl buffer which did not alter insulin binding, but gave much higher yields than density gradient methods. Mouse erythrocytes were isolated from heparinized...


    pub_type: 杂志文章


    authors: Shimizu F,Kahn CR

    更新日期:1982-02-01 00:00:00

  • Estradiol stimulation of c-fos and c-jun expressions and activator protein-1 deoxyribonucleic acid binding activity in rat white adipocyte.

    abstract::In order to elucidate the molecular mechanisms whereby ovarian hormones, and particularly estrogens, modulate fat cell metabolism, we investigated the effects of estradiol administration on c-fos and c-jun expressions in fat cells from ovariectomized (OVX) rats. Estradiol treatment resulted in a rapid increase in c-fo...


    pub_type: 杂志文章


    authors: Garcia E,Lacasa D,Giudicelli Y

    更新日期:2000-08-01 00:00:00

  • Structural manifestations of the rat Sertoli cell to hypophysectomy: a correlative morphometric and endocrine study.

    abstract::Although the Sertoli cell is a key cell mediating the actions of FSH- and LH-stimulated testosterone (T) in the testis, there is little information to indicate how this cell responds structurally to hormonal insufficiency. The present study used morphometric techniques to study the structural manifestations of the Ser...


    pub_type: 杂志文章


    authors: Ghosh S,Bartke A,Grasso P,Reichert LE Jr,Russell LD

    更新日期:1992-07-01 00:00:00

  • Chronic Exposure to TNFα Impairs Secretion of Glucagon-Like Peptide-1.

    abstract::Obesity is associated with systemic inflammation and elevated levels of TNFα, leading to impaired glucose tolerance. In humans, obesity is also associated with reduced nutrient-stimulated secretion of the intestinal incretin hormone, glucagon-like peptide-1 (GLP-1). We hypothesized that TNFα plays a direct role in the...


    pub_type: 杂志文章


    authors: Gagnon J,Sauvé M,Zhao W,Stacey HM,Wiber SC,Bolz SS,Brubaker PL

    更新日期:2015-11-01 00:00:00

  • Effect of osmolarity on aldosterone production by rat adrenal glomerulosa cells.

    abstract::The effect of osmotic changes on aldosterone production, [Ca2+]i and voltage-gated Ca2+ currents, was studied in cultured rat glomerulosa cells. Alteration of osmolarity by sucrose addition in the 250-330 mosM range did not influence aldosterone production per se, but it substantially affected K+-stimulated aldosteron...


    pub_type: 杂志文章


    authors: Makara JK,Petheö GL,Tóth A,Spät A

    更新日期:2000-05-01 00:00:00

  • Reevaluation of the role of cyclic adenosine 3',5'-monophosphate and protein kinase in the stimulation of steroidogenesis by luteinizing hormone in bovine corpus luteum slices.

    abstract::Incubation of bovine corpus luteum slices at 37 C with luteinizing hormone showed that 10.0 microng LH/ml caused a maximum rise in tissue cyclic AMP content within 15 min. Slices incubated with 1.0 or 0.1 microng LH/ml showed a much more gradual accumulation of this nucleotide. In the absence of added LH, a marked dec...


    pub_type: 杂志文章


    authors: Ling WY,Marsh JM

    更新日期:1977-06-01 00:00:00

  • Marked seasonal changes in response to the negative feedback action of estradiol on luteinizing hormone secretion in the female Japanese monkey.

    abstract::The seasonal changes in response to the negative feedback action of estradiol on LH secretion were studied in two groups of ovariectomized Japanese monkeys (Macaca fuscata fuscata; n = 6). Blood samples were collected two to three times a week, and changes in serum concentrations of LH, estradiol, and progesterone wer...


    pub_type: 杂志文章


    authors: Nozaki M,Watanabe G,Taya K

    更新日期:1991-03-01 00:00:00

  • Metabolic clearance of endogenous and radioiodinated thyroglobulin in rats.

    abstract::The basal concentration and metabolic clearance of radiolabeled and endogenous thyroglobulin (Tg) were measured in normal male rats. Serum Tg concentrations (by RIA) in Sprague-Dawley adult males ranged from 70-680 ng/ml (mean +/- SD, 218 +/- 177). In male Fisher rats, basal serum Tg concentrations were 32-263 ng/ml. ...


    pub_type: 杂志文章


    authors: Izumi M,Larsen PR

    更新日期:1978-07-01 00:00:00

  • Down regulation of insulin receptors in primary cultures of adult rat hepatocytes in monolayer.

    abstract::Down regulation of insulin receptor number by insulin at physiological concentrating (10(-9) M) has been demonstrated in primary cultures of adult rat liver parenchymal cells in monolayer. However, because insulin is rapidly degraded in this culture system, special efforts are necessary to maintain the concentration o...


    pub_type: 杂志文章


    authors: Blackard WG,Guzelian PS,Small ME

    更新日期:1978-08-01 00:00:00

  • Electrical responses of rat islets maintained in culture with varying levels of glucose.

    abstract::The electrical responses of rat islet cells maintained in culture with varying levels of glucose were determined. After culture with 2.8 or 4.2 mM glucose, the beta-cells did not respond electrically to 27.8 mM glucose. However, after culture in 5.6 mM glucose, the cells responded to 27.8 mM glucose in short term expe...


    pub_type: 杂志文章


    authors: Pace CS,Conant S,Lacy PE

    更新日期:1978-05-01 00:00:00

  • Acute effects of estradiol on circulating luteinizing hormone and prolactin concentrations and on serotonin turnover in individual brain nuclei.

    abstract::Two experiments tested whether serotonergic neurons participate in the acute feedback effects of estradiol on LH and PRL secretion. In the first study, ovariectomized female rats received oil vehicle or estradiol benzoate and were killed 3 h later. The turnover of serotonin [5-hydroxytryptamine (5-HT)] in microdissect...


    pub_type: 杂志文章


    authors: Johnson MD,Crowley WR

    更新日期:1983-12-01 00:00:00

  • Estrogen stimulation of cell migration involves multiple signaling pathway interactions.

    abstract::Hormone-dependent breast cancers respond to inhibitors of estrogen synthesis or action with tumor regression and with a reduction of new metastases. The mechanisms underlying the effects of estrogen on metastasis likely differ from those on tumor regression. Cell migration is a key first step in the metastatic process...


    pub_type: 杂志文章


    authors: Li Y,Wang JP,Santen RJ,Kim TH,Park H,Fan P,Yue W

    更新日期:2010-11-01 00:00:00

  • Bradykinin stimulates aldosterone release from cultured bovine adrenocortical cells through bradykinin B2 receptors.

    abstract::The adrenal cortex contains a kallikrein-like enzyme that may lead to bradykinin (BK) formation. This study was designed to determine whether BK acts on adrenocortical cells to stimulate steroid secretion. BK, Lys-BK, a specific BK 2 (B2) receptor agonist, and desArg9-BK, a specific BK 1 (B1) receptor agonist, all sti...


    pub_type: 杂志文章


    authors: Rosolowsky LJ,Campbell WB

    更新日期:1992-04-01 00:00:00