Bone re/modeling is more dynamic in the endothelial nitric oxide synthase(-/-) mouse.


:Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO synthase (eNOS) in bone cells and bone progenitor cells at regular intervals up to 10 wk after acute estrogen deprivation. Ovariectomy led to an anticipated initial decline in bone cell eNOS production, but surprisingly, 17 d after ovariectomy, eNOS expression by bone and marrow stromal cells dramatically rebounded and was maintained at high levels for at least 10 wk after surgery. We examined the long-term consequences of eNOS in the process of ovariectomy-induced bone loss by prospectively analyzing bone mineral density in wild-type and eNOS(-/-) mice for 10 wk after ovariectomy. Ovariectomized eNOS(-/-) mice were observed to undergo an exaggerated state of estrogen-deficiency-induced bone remodeling compared with wild-type controls, suggesting that eNOS may act to mitigate this process. Furthermore, we found that whereas bone formation in estrogen-replete wild-type mice slowed between 14 and 20 wk of age, eNOS knockout mice continued to accrue basal bone mass at a high rate and showed no sign of entering a remodeling stage. Our data suggest that eNOS may play an important role in limiting ovariectomy-induced bone remodeling as well as regulating the transition from basal modeling to remodeling.






Grassi F,Fan X,Rahnert J,Weitzmann MN,Pacifici R,Nanes MS,Rubin J




Has Abstract


2006-09-01 00:00:00














  • Growth hormone (GH) secretion in the pituitary-grafted male rat: in vivo effects of GH-releasing hormone and isoproterenol and in vitro release by individual somatotropes.

    abstract::Although the pituitary-grafted rat is a classic model of chronic PRL excess, the presence of somatotropes in grafted pituitary tissue indicates a potential for GH secretion. The current study was designed to investigate GH-releasing hormone (GRH)-induced GH secretion and beta-adrenergic inhibition of GH release in ani...


    pub_type: 杂志文章


    authors: Krieg RJ Jr,Johnson JH,Adler RA

    更新日期:1989-11-01 00:00:00

  • See-saw signal processing: reciprocal effects of stimulus deprivation on vasoactive intestinal peptide-stimulated adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate accumulation in rat pinealocytes.

    abstract::In the present study the effects of stimulus deprivation on vasoactive intestinal peptide (VIP)- and alpha 1-adrenergically mediated amplification of VIP-stimulated cAMP and cGMP accumulation were examined. Dispersed pinealocytes were prepared from either Sprague-Dawley rats maintained for 2 weeks in a normal lighting...


    pub_type: 杂志文章


    authors: Chik CL,Ho AK

    更新日期:1991-02-01 00:00:00

  • Cloning and expression of kiss2 in the zebrafish and medaka.

    abstract::Newly discovered kisspeptin (metastin), encoded by the Kiss1/KISS1 gene, is considered as a major gatekeeper of puberty through the regulation of GnRH. In the present study, we cloned a novel kisspeptin gene (kiss2) in the zebrafish Danio rerio and the medaka Oryzias latipes, which encodes a sequence of 125 and 115 am...


    pub_type: 杂志文章


    authors: Kitahashi T,Ogawa S,Parhar IS

    更新日期:2009-02-01 00:00:00

  • The pharmacokinetics of thyrotropin-releasing hormone (TRH) and deamido-TRH in the rat.

    abstract::The pharmacokinetics of TRH and its metabolite deamido-TRH (TRH-OH) were evaluated and compared. After a 60- to 90-min infusion of the appropriate peptide into rats, the MCR, half-life of disappearance (t 1/2), and volume of distribution were calculated. The MCR of TRH averaged 4 ml/min, whereas that of TRH-OH was 6.5...


    pub_type: 杂志文章


    authors: Safran M,Wu CF,Matys R,Alex S,Emerson CH

    更新日期:1984-09-01 00:00:00

  • Developmental changes in epithelial cadherin messenger ribonucleic acid and immunocytochemical localization of epithelial cadherin during postnatal epididymal development in the rat.

    abstract::The formation of junctional complexes between adjacent epithelial principal cells leads to formation of the blood-epididymal barrier; this barrier is complete by 21 days of postnatal age. Cadherins are cell surface proteins that mediate intercellular adhesion and are involved in the formation of adherence, gap, and ti...


    pub_type: 杂志文章


    authors: Cyr DG,Hermo L,Robaire B

    更新日期:1993-03-01 00:00:00

  • First intron excision of GnRH pre-mRNA during postnatal development of normal mice and adult hypogonadal mice.

    abstract::Previously, we demonstrated that excision of the GnRH first intron (intron A) was largely attenuated in non-GnRH-producing tissues but accelerated in GnRH neurons. In the present study, we examined the splicing rate of GnRH pre-mRNA in developing normal mice and adult hypogonadal mice. The preoptic area and cerebral c...


    pub_type: 杂志文章


    authors: Seong JY,Kim BW,Park S,Son GH,Kim K

    更新日期:2001-10-01 00:00:00

  • Expression and biological activity of parathyroid hormone-related peptide in pregnant rat uterine artery: any role for 8-iso-prostaglandin F2alpha?

    abstract::PTHrP is produced in vessels and acts as a local modulator of tone. We recently reported that PTHrP(1-34) is able to induce vasorelaxation in rat uterine arteries, but in pregnancy, this response is blunted and becomes strictly endothelium dependent. The present study aimed to get insights into the mechanisms involved...


    pub_type: 杂志文章


    authors: Meziani F,Tesse A,Welsch S,Kremer H,Barthelmebs M,Andriantsitohaina R,Schneider F,Gairard A

    更新日期:2008-02-01 00:00:00

  • Triiodothyronine inhibits phorbol ester-induced thyrotropin release from elutriated bovine thyrotrophs.

    abstract::The inhibitory effect of T3 on TSH release was studied on a population of thyrotroph-enriched cells prepared from bovine pituitary glands by centrifugal elutriation. The cells (2.0 X 10(5)/ml) were cultured for 2 days and then exposed to TRH, phorbol-12 myristate-13 acetate (PMA), or calcium ionophore (A23187) with or...


    pub_type: 杂志文章


    authors: Yanagisawa M,Mirell CJ,Hershman JM

    更新日期:1988-05-01 00:00:00

  • Effect of antibodies to estrogen receptor on the binding of 3H-labeled antiestrogens and androstanediol in the uterus.

    abstract::We report here the effects of antibodies to estrogen receptor (ER) on the binding of tritiated 5 alpha-androstane-3 beta-17 beta-diol and antiestrogens to receptor in calf uterine cytosol. The antibodies, prepared from a rabbit immunized with purified estradiol-receptor complex from calf uterine nuclei, were found to ...


    pub_type: 杂志文章


    authors: Garcia M,Greene G,Rochefort H,Jensen EV

    更新日期:1982-04-01 00:00:00

  • Regulation and actions of Smad7 in the modulation of activin, inhibin, and transforming growth factor-beta signaling in anterior pituitary cells.

    abstract::Activins and transforming growth factor-beta (TGF beta) are crucial autocrine, paracrine, and endocrine modulators of anterior pituitary function. Activins regulate most pituitary cells and lactotropes are targets of TGF beta. Smad2 and Smad3 are two cellular mediators of activin/TGF beta signaling, whereas Smad7 is a...


    pub_type: 杂志文章


    authors: Bilezikjian LM,Corrigan AZ,Blount AL,Chen Y,Vale WW

    更新日期:2001-03-01 00:00:00

  • Prostaglandin F2 alpha mediates ovarian sterol carrier protein-2 expression during luteolysis.

    abstract::In the corpus luteum, prostaglandin F2 alpha (PGF2 alpha) appears to be a physiological agent with both antisteroidogenic and luteolytic actions. It is hypothesized that the antisteroidogenic action of PGF2 alpha acts through altered transport of cholesterol to the mitochondrial cytochrome P450 side-chain cleavage enz...


    pub_type: 杂志文章


    authors: McLean MP,Billheimer JT,Warden KJ,Irby RB

    更新日期:1995-11-01 00:00:00

  • Ultrastructural localization of relaxin immunoreactivity in corpora lutea of pregnant rats.

    abstract::Relaxin was localized in cells of corpora lutea of pregnant rats at the ultrastructural level using a highly specific antirat relaxin serum and the unlabeled peroxidase-antiperoxidase technique. Electron-dense, membrane-bound granules (maximum diameter, 270 nm), which are present in luteal cells during the last third ...


    pub_type: 杂志文章


    authors: Anderson MB,Sherwood OD

    更新日期:1984-04-01 00:00:00

  • An irradiation-altered bone marrow microenvironment impacts anabolic actions of PTH.

    abstract::PTH stimulates bone formation and increases hematopoietic stem cells through mechanisms as yet uncertain. The purpose of this study was to identify mechanisms by which PTH links actions on cells of hematopoietic origin with osteoblast-mediated bone formation. C57B6 mice (10 d) were nonlethally irradiated and then admi...


    pub_type: 杂志文章


    authors: Koh AJ,Novince CM,Li X,Wang T,Taichman RS,McCauley LK

    更新日期:2011-12-01 00:00:00

  • Expression of vesicular glutamate transporter-2 in gonadotropin-releasing hormone neurons of the adult male rat.

    abstract::Isoforms of the recently cloned vesicular glutamate transporters (VGLUT1-3) selectively accumulate glutamic acid into synaptic vesicles in excitatory axon terminals and are viewed as reliable markers for glutamatergic neurons. Our present studies provided dual-label in situ hybridization evidence that virtually all (9...


    pub_type: 杂志文章


    authors: Hrabovszky E,Turi GF,Kalló I,Liposits Z

    更新日期:2004-09-01 00:00:00

  • 11 beta-Hydroxysteroid dehydrogenase type 2 complementary deoxyribonucleic acid stably transfected into Chinese hamster ovary cells: specific inhibition by 11 alpha-hydroxyprogesterone.

    abstract::The 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta HSD-2) enzyme is thought to confer aldosterone specificity upon mineralocorticoid target tissues by protecting the mineralocorticoid receptor from binding by the more abundant glucocorticoids, corticosterone and cortisol. We have developed a Chinese hamster ovar...


    pub_type: 杂志文章


    authors: Morita H,Zhou M,Foecking MF,Gomez-Sanchez EP,Cozza EN,Gomez-Sanchez CE

    更新日期:1996-06-01 00:00:00

  • Effects of bombesin on pancreatic digestive enzyme gene expression.

    abstract::We examined the effects of bombesin on rat pancreatic digestive enzyme gene expression using cloned complementary DNA probes for amylase, trypsinogen I, chymotrypsinogen B, and lysophospholipase. Rats were injected sc three times daily with 5 nmol/kg body wt bombesin. Pancreata were investigated after 6, 12, 24, 48, a...


    pub_type: 杂志文章


    authors: Rosewicz S,Liehr RM,Solomon TE,Riecken EO,Logsdon CD

    更新日期:1992-03-01 00:00:00

  • Bfk, a novel member of the bcl2 gene family, is highly expressed in principal cells of the mouse epididymis and demonstrates a predominant nuclear localization.

    abstract::B-cell lymphoma 2 (BCL2) family kin (BFK) is a recently identified novel protein that is similar to proteins of the BCL2 family. In the present study, we discovered that the mouse Bfk transcript is expressed at the highest level in the epididymis. Two transcripts of 0.9 and 2.6 kb in size were identified, with alterna...


    pub_type: 杂志文章


    authors: Pujianto DA,Damdimopoulos AE,Sipilä P,Jalkanen J,Huhtaniemi I,Poutanen M

    更新日期:2007-07-01 00:00:00

  • Failure of prolactin short loop feedback mechanism to operate in old as compared to young female rats.

    abstract::The short loop feedback effect of PRL was studied in young (4-5 months of age) and old (24-26 months of age) ovariectomized rats after a single iv injection of bovine PRL (bPRL, 500 micrograms/100 g BW) or BSA (500 micrograms/100 g BW). Blood samples were collected via intraatrial cannula every 20 min for assay of PRL...


    pub_type: 杂志文章


    authors: Sarkar DK,Miki N,Meites J

    更新日期:1983-10-01 00:00:00

  • Vascular endothelial growth factor receptor-2 activation induces vascular permeability in hyperstimulated rats, and this effect is prevented by receptor blockade.

    abstract::The existence of a vasoactive molecule released in response to hCG is believed to be the main feature in the development of ovarian hyperstimulation syndrome (OHSS) in women, and vascular endothelial growth factor (VEGF) is the main candidate as the human chorionic gonadotropin (hCG) mediator. This study was conducted...


    pub_type: 杂志文章


    authors: Gómez R,Simón C,Remohí J,Pellicer A

    更新日期:2002-11-01 00:00:00

  • Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary.

    abstract::Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains cha...


    pub_type: 杂志文章


    authors: Jacobs S,Calebiro D,Nikolaev VO,Lohse MJ,Schulz S

    更新日期:2010-09-01 00:00:00

  • Pancreastatin: molecular and immunocytochemical characterization of a novel peptide in porcine and human tissues.

    abstract::Pancreastatin, a novel 49-amino acid peptide isolated from porcine pancreas, shows over 70% sequence homology to the central part of bovine and human chromogranin-A. Using an N-terminal and C-terminal synthetic peptide, we developed two sensitive and specific RIAs for the detection of pancreastatin-like immunoreactivi...


    pub_type: 杂志文章


    authors: Schmidt WE,Siegel EG,Lamberts R,Gallwitz B,Creutzfeldt W

    更新日期:1988-09-01 00:00:00

  • Prolactin restores plasma testosterone levels and stimulates testicular growth in hamsters exposed to short day-length.

    abstract::In male hamsters, light deprivation was reported to reduce both testis weight and prolactin (PRL) levels. Therefore, we decided to examine the effects of PRL on testicular function in hamsters exposed to short day-length. Adult hamsters were exposed to 5 h of light per day to induce gonadal atrophy, and, starting two ...


    pub_type: 杂志文章


    authors: Bartke A,Croft BT,Dalterio S

    更新日期:1975-12-01 00:00:00

  • Steroid-binding proteins in guinea pig milk and plasma.

    abstract::The levels of two steroid-binding proteins, progesterone-binding globulin (PBG) (1) and corticosteroid-binding globulin (CBG), present in the plasma of pregnant guinea pigs were determined just before and after parturition. Both PBG (3.37 +/- 2.1 microM) and CBG (10.1 +/- 1.7 microM) were present in high levels just b...


    pub_type: 杂志文章


    authors: Raymoure WJ,Kuhn RW

    更新日期:1980-06-01 00:00:00

  • Effect of estrogen on adenosine 3'5',cyclic monophosphate in quail oviduct: possible involvement in estradiol-activated growth.

    abstract::Previous studies have shown that estradiol indirectly stimulated the proliferation of oviduct epithelial cells in the quail. The present study was undertaken to investigate the effects of estradiol and other steroid hormones on the cAMP concentration. The ability of forskolin, a specific activator of the catalytic sub...


    pub_type: 杂志文章


    authors: Laugier C,Courion C,Pageaux JF,Fanidi A,Dumas MY,Sandoz D,Nemoz G,Prigent AF,Pacheco H

    更新日期:1988-01-01 00:00:00

  • Cysteamine induces depletion of both immunological and biological prolactin activity in the anterior pituitary and blood of the rat.

    abstract::Previous studies have shown that cysteamine [2-aminoethanethiol (CSH)], given in vivo or in vitro, rapidly but reversibly depletes immunoreactive PRL from the anterior pituitary (AP) through a mechanism which, in vitro, does not appear to involve the dopamine (DA) receptor. In the present investigation, these effects ...


    pub_type: 杂志文章


    authors: Millard WJ,Sagar SM,Simpkins JW,Owens RE,Badger TM,Friesen HG,Martin JB

    更新日期:1983-12-01 00:00:00

  • In pursuit of leucine-rich repeat-containing G protein-coupled receptor-5 regulation and function in the uterus.

    abstract::Leucine-rich repeat-containing G protein-coupled receptor (LGR)-5 is a recently identified marker of stem cells in adult intestinal epithelium and hair follicles. Because of this characteristic, we studied the status of Lgr5 expression in the mouse uterus under various conditions. Lgr5 is highly expressed in the uteri...


    pub_type: 杂志文章


    authors: Sun X,Jackson L,Dey SK,Daikoku T

    更新日期:2009-11-01 00:00:00

  • Dopamine and L-dopa: inhibition of thyrotropin-stimulated thyroidal thyroxine release.

    abstract::Previous studies had suggested that norepinephrine (NE) and its precursors dopamine (DA) and L-DOPA acted similarly on iodine metabolism of isolated thyroid cells. Present studies indicate that this similarity extends to the inhibition by catecholamines of TSH-stimulated T4 release by mouse thyroids incubated in vitro...


    pub_type: 杂志文章


    authors: Maayan ML,Sellitto RV,Volpert EM

    更新日期:1986-02-01 00:00:00

  • Insulin induced alterations of acyl groups of the cerebroside fraction isolated from fat cell ghosts.

    abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...


    pub_type: 杂志文章


    authors: Murray DK,Nelson DH

    更新日期:1981-05-01 00:00:00

  • Influence of prenatal photoperiods on postnatal reproductive responses to daily infusions of melatonin in the Siberian hamster (Phodopus sungorus).

    abstract::Prepubertal reproductive development in juvenile male Siberian hamsters can be strongly influenced by photoperiod information received during gestation. Information transmitted from the mother hamster to her fetuses appears to modify the photoperiodic mechanism of the developing hamsters so that they may respond diffe...


    pub_type: 杂志文章


    authors: Shaw D,Goldman BD

    更新日期:1995-10-01 00:00:00

  • Identification of biologically active epidermal growth factor precursor in human fluids and secretions.

    abstract::A biologically active form of the epidermal growth factor (EGF) precursor has been detected in human fluids and secretions. The secreted protein identified in human urine and milk has an apparent molecular mass of 160-170 kilodaltons and exhibits an affinity for the glycosaminoglycan heparin. More importantly, the sec...


    pub_type: 杂志文章


    authors: Mroczkowski B,Reich M

    更新日期:1993-01-01 00:00:00