Abstract:
:We have developed a highly sensitive and specific RIA for propylthiouracil (PTU) which uses 125I-labeled PTU as the radioactive ligand. At a final antibody dilution of 1:10,000, the detection limit for PTU was 100 pg; cross-reactivity with circulating, urinary, and intrathyroid PTU metabolites was negligible. Using this assay, serum and thyroid PTU levels were determined after short term (1 week) and long term (1 month) PTU treatment at doses of 0.0001-0.05%. Serum PTU was a linear function of the PTU dose (r = 0.99; P less than 0.001), whereas thyroid PTU was a linear function of the logarithm of the PTU dose (r = 0.99; P less than 0.001). Serum PTU levels were higher after 1 month of treatment than after administration for 1 week, probably because steady state conditions were not achieved after 1 week. At several doses, thyroid PTU levels were also higher after 1 month of treatment, but the differences were not as striking as those seen in the serum levels. The pharmacokinetic data are consistent with a multicompartmental model for PTU distribution. The logarithmic relationship between thyroid PTU and PTU dose suggests a saturable uptake mechanism for PTU by the thyroid; inhibition of thyroid PTU uptake by PTU itself could also explain these observations.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Halpern R,Cooper DS,Kieffer JD,Saxe V,Mover H,Maloof F,Ridgway ECdoi
10.1210/endo-113-3-915subject
Has Abstractpub_date
1983-09-01 00:00:00pages
915-20issue
3eissn
0013-7227issn
1945-7170journal_volume
113pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Neonatal thymectomy at 3 days of age in mice results in ovarian dysgenesis, characterized by early follicular and oocyte loss, followed by a high incidence of ovarian tumorigenesis. Thymectomized animals with dysgenetic ovaries had extremely high levels of circulating PRL and gonadotropins before the appearance of the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-6-2375
更新日期:1981-06-01 00:00:00
abstract::Brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP) are present in brain regions regulating LH secretion. Similarities in the molecular structure of these peptides suggest similar physiological function within the brain. This study examined the effects of centrally administered BNP and ANP on LH secre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-801
更新日期:1991-08-01 00:00:00
abstract::Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunoh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8588
更新日期:2002-01-01 00:00:00
abstract::We have previously demonstrated that, in prostate cancer cells, androgens up-regulate IGF-I receptor (IGF-IR) by inducing cAMP-response element-binding protein (CREB) activation and CREB-dependent IGF-IR gene transcription through androgen receptor (AR)-dependent membrane-initiated effects. This IGF-IR up-regulation i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1925
更新日期:2014-04-01 00:00:00
abstract::A healthy nutritional state is required for all aspects of reproduction and is signaled by the adipokine leptin. Leptin acts in a relatively narrow concentration range: too much or too little will compromise fertility. The leptin signal timing is important to prepubertal development in both sexes. In the brain, leptin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa204
更新日期:2021-02-01 00:00:00
abstract::The proliferation and differentiation of testicular progenitor stem cells into highly specialized germ cells (spermatozoa) are largely controlled by the hormonally (FSH and testosterone) regulated adjacent supporting Sertoli cells. However, the factors involved in this control remain largely unknown. In the present st...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.8.7625
更新日期:2000-08-01 00:00:00
abstract::In a previous study we found that PTH stimulated bone resorption and release of insulin-like growth factor-I (IGF-I) and IGF-II from cultured neonatal mouse calvaria. Since IGF-I and IGF-II stimulate osteoblast proliferation and collagen synthesis, these results suggested that increased release of IGFs during resorpti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1511
更新日期:1991-03-01 00:00:00
abstract::LH treatment of isolated medium (3- to 7-mm) follicles from porcine ovaries stimulated pregnenolone synthesis by mitochondria isolated from these tissues. This LH stimulation of pregnenolone synthesis was observed regardless of whether exogenous cholesterol was added to the mitochondrial assay system, suggesting that ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-588
更新日期:1984-02-01 00:00:00
abstract::Acidosis has been implicated in the pathogenesis of various metabolic bone diseases. Experimental acid ingestion is known to stimulate the release of bone mineral in humans and increase osteoclastic resorption surfaces in animals, but the mechanism has remained unclear. We have investigated the effect of medium pH on ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-1-119
更新日期:1986-07-01 00:00:00
abstract::We have investigated the role of protein kinase C (PKC) isoenzymes in the differential growth regulation of human pancreatic carcinoma cell lines by all-trans retinoic acid (RA). RA treatment results in dose-dependent stimulation of anchorage-independent growth in AsPc1 cells and growth inhibition in Capan 2 cells. Bo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754760
更新日期:1996-08-01 00:00:00
abstract::The present studies were performed to determine whether the major biologically active metabolite of vitamin D3, 1,25-dihydroxycholecalciferol [1,25(OH)2D3], could influence the activities of rat colonic particulate guanylate cyclase and adenylate cyclase. To address these issues, colonocytes were harvested from Spragu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8104780
更新日期:1993-11-01 00:00:00
abstract::The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-178
更新日期:1983-07-01 00:00:00
abstract::Compelling evidence indicates a crucial role of prostaglandin F2α (PGF2α) in parturition. Both the maternal and fetal sides of the fetal membranes synthesize PGF2α, which exerts effects via the prostaglandin F2α receptor (FP) that is coupled to the activation of protein kinase C (PKC). Cyclooxygenase-2 (COX-2) catalyz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1441
更新日期:2012-10-01 00:00:00
abstract::A member of the interferon-inducible p200 family of proteins, p204, has recently been reported to function in the development of many mesoderm-derived tissues, such as bone, muscle, and cartilage. However, no published study has yet investigated the role of p204 in adipogenesis. Our preliminary experiments showed that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1381
更新日期:2010-07-01 00:00:00
abstract::The relationship between serum levels of rat GH (rGH), rat PRL (rPRL), corticosterone, estrogen, and testosterone and the liver-binding sites specific to [125I]iodo-human GH (hGH), -human PRL (hPRL), and -rPRL were investigated in normal rats, in rats bearing the GH- and PRL-secreting tumor (GH3), and in rats 14 days ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2053
更新日期:1978-12-01 00:00:00
abstract::The present study was undertaken to evaluate the efficacy of an estradiol-chemical delivery system (E2-CDS) for the brain vs. estradiol benzoate (E2-BNZ) in suppressing serum testosterone (T) and weights of the ventral prostate and seminal vesicle in male rats. Also, the role of serum T in the weight reduction of andr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-726
更新日期:1991-08-01 00:00:00
abstract::Estrogen regulates the function, growth, and proliferation of lactotropes in the pituitary. We report here that low-density lipoprotein (LDL) receptor (LDLR) gene expression and LDL uptake are strongly up-regulated by estrogen in pituitary somatolactotropic GH(3) cells. The uptake of LDL was significantly inhibited by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0228
更新日期:2004-07-01 00:00:00
abstract::The scaffolding protein, protein targeting to glycogen (PTG), orchestrates the signaling of several metabolic enzymes involved in glycogen synthesis. However, little is known concerning the regulation of PTG itself. In this study, we have cloned and characterized the mouse promoter of PTG. We identified multiple FoxA2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1513
更新日期:2006-07-01 00:00:00
abstract::Depletion of hypothalamic norepinephrine (NE) and epinephrine by administration of diethyldithiocarbamate abolished the stimulatory effects of intraventricular (IVT) angiotensin II (AII) on LH release in ovariectomized rats pretreated with estradiol and progesterone. The increase in blood LH produced by IVT NE or iv L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2728
更新日期:1986-12-01 00:00:00
abstract::The secretion of LH, PRL, and cortisol was investigated in 4 sexually mature female rhesus macaques with cardiac catheters protected by tethers. Based on endocrine parameters, all 4 of the animals ovulated within 2 months from the time they were tethered, and regular menstrual cycles of 24-34 days were observed. The c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-261
更新日期:1984-07-01 00:00:00
abstract::Experiments were carried out to characterize the prolactin stimulation of labeled uridine uptake into mammary gland explants which were initially preincubated for 2 days in medium containing insulin plus hydrocortisone. Prolactin was found to enhance labeled uridine uptake after 4 h but not after 2 h when uridine conc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-5-1307
更新日期:1975-05-01 00:00:00
abstract::In the murine Ob 17 preadipocyte cell line, the thyroid hormone T3 is an adipogenic factor necessary at an early stage for differentiation into adipocyte. We demonstrate here that this T3 dependence may involve a transient expression (at both the messenger RNA and the protein levels) of c-ErbA beta-type receptors (T3R...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.7.6860
更新日期:1999-07-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) enhances insulin biosynthesis and secretion as well as transcription of the insulin, GLUT2 and glucokinase genes. The latter are also regulated by the PDX-1 homeoprotein. We investigated the possibility that GLP-1 may be having its long-term pleiotropic effects through a hitherto unknow...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7158
更新日期:1999-10-01 00:00:00
abstract::A series of rat and mouse monoclonal antibodies to the human progesterone receptor (PR) has recently been produced. These antibodies were used for the immunocytochemical identification of PR in several mammalian species including humans. The specificity of the monoclonal antibodies for PR was confirmed by using compet...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-3-1165
更新日期:1988-03-01 00:00:00
abstract::The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.7689950
更新日期:1993-09-01 00:00:00
abstract::Brown adipose tissue (BAT) thermogenesis is activated by the sympathetic nervous system. BAT responses to norepinephrine are blunted in hypothyroidism and are rapidly restored by thyroid hormone. We examined in rats the effects of thyroid hormone on BAT beta 1- and beta 2-adrenergic receptors (AR) expression and capac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628360
更新日期:1995-08-01 00:00:00
abstract::The multifunctional cytokine, transforming growth factor-beta (TGF beta), is found in many tissues in a latent or inactive form. The nature and composition of the latent complex can vary depending on tissue type. The release of active TGF beta from its latent complex is a potentially important mechanism for regulation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4944
更新日期:1997-02-01 00:00:00
abstract::Multiple forms of PRL differing in their physicochemical and biological characteristics have been described. We have analyzed the molecular forms of human (h) PRL released in culture by pure hPRL-secreting tumors with a particular attention to glycosylated hPRL. The prolactinoma cells from six different tumors release...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2667
更新日期:1988-06-01 00:00:00
abstract::The aim of this study was to characterize the dynamics and functional relevance of interleukin-1 beta (IL-1 beta)-induced nitric oxide production in isolated pancreatic islets. Thus, islets were isolated from adult rats, precultured for 3-5 days in medium RPMI-1640 plus 10% fetal calf serum, and then exposed to IL-1 b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3167
更新日期:1991-12-01 00:00:00
abstract::Bone morphogenetic protein (BMP)-4 is a key mediator of anterior pituitary organogenesis. However, through inappropriate expression patterns, BMP-4 is also pathogenic in a pituitary adenoma subtype-specific context. In these cases, increase or decrease in BMP-4 in lactotroph- and corticotroph-derived adenomas, respect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1231
更新日期:2012-08-01 00:00:00