Hormone release by islet B cell-enriched and A and D cell-enriched populations prepared by flow cytometry.

abstract：:We investigated the effect of epidermal growth factor (EGF) on collagen and protein synthesis in clone MC3T30-E1, a cell line which retains osteoblast-like characteristics. EGF at concentrations of 2-50 ng/ml significantly the hydroxyproline content of the cell layer. These effects were completely abolished by the add...

journal_title：Endocrinology

authors： Hiramatsu M,Kumegawa M,Hatakeyama K,Yajima T,Minami N,Kodama H

更新日期：1982-12-01 00:00:00

abstract：:Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...

journal_title：Endocrinology

authors： Murray DK,Nelson DH

更新日期：1981-05-01 00:00:00

abstract：:A rat medullary thyroid carcinoma, which was previously shown to produce high levels of immunoreactive cholecystokinin (CCK), was used to establish a stable cell line. Transplantable tumors were subjected to four series of alternate in vitro and in vivo passages. Cells were prepared from the fourth series of tumors un...

journal_title：Endocrinology

authors： Haun RS,Beinfeld MC,Roos BA,Dixon JE

更新日期：1989-08-01 00:00:00

abstract：:A variety of chronic stress paradigms have been shown to increase basal activity in the hypothalamic-pituitary-adrenocortical axis, resulting in hypercorticoidism. Despite this, chronically stressed rats typically exhibit facilitated ACTH responses to acute novel stress, suggesting that the activity of some central ne...

journal_title：Endocrinology

authors： Scribner KA,Akana SF,Walker CD,Dallman MF

更新日期：1993-12-01 00:00:00

abstract：:GH and insulin-like growth factor (IGF)-I are important components of the growth axis. We undertook to determine how plasma levels of these hormones altered with different seasonal and nutritional states in young male red deer to provide an insight into how the growth axis changes under these conditions. Growth rate a...

journal_title：Endocrinology

authors： Webster JR,Corson ID,Littlejohn RP,Stuart SK,Suttie JM

更新日期：1996-02-01 00:00:00

abstract：:The insulin-like growth factor-binding proteins (IGFBPs) comprise at least six distinct species that may modulate the action of IGFs. IGFs are important regulators of fetal growth and differentiation. To define sites of IGFBP mRNA synthesis, we have used in situ hybridization techniques in mouse conceptuses of differe...

journal_title：Endocrinology

authors： Schuller AG,Zwarthoff EC,Drop SL

更新日期：1993-06-01 00:00:00

abstract：:The circadian rhythm of glucocorticoids affects diverse physiological systems, including stress responses and the coordination of rhythmic functions in peripheral and central tissues. Circadian clocks are considered to be important coordinators of glucocorticoid release and loss of the core clock component Brain and m...

journal_title：Endocrinology

authors： Dumbell R,Leliavski A,Matveeva O,Blaum C,Tsang AH,Oster H

更新日期：2016-11-01 00:00:00

abstract：:Oxytocin stimulates phosphoinositide turnover in myometrium. To elucidate whether the coupling mechanism involves the interaction of oxytocin receptor with GTP-binding proteins, we examined oxytocin stimulation of guanosine triphosphatase (GTPase) activity and phospholipase-C activity in rat and human myometrial membr...

journal_title：Endocrinology

authors： Ku CY,Qian A,Wen Y,Anwer K,Sanborn BM

更新日期：1995-04-01 00:00:00

abstract：:GH is known to increase the formation of bone and hard tissues of the tooth (dentine, cementum, and enamel), as do bone morphogenetic proteins. GH receptors are expressed in these tissues and could mediate local growth responses. Here we report that both GH and insulin-like growth factor I (IGF-I) are able to increase...

journal_title：Endocrinology

authors： Li H,Bartold PM,Zhang CZ,Clarkson RW,Young WG,Waters MJ

更新日期：1998-09-01 00:00:00

abstract：:Loss of circulating 17β-estradiol (E2) that occurs during menopause can have detrimental effects on cognitive function. The efficacy of hormone replacement therapy declines as women become farther removed from the menopausal transition, yet the molecular mechanisms underlying this age-related switch in E2 efficacy are...

journal_title：Endocrinology

authors： Shults CL,Pinceti E,Rao YS,Pak TR

更新日期：2015-11-01 00:00:00

abstract：:The antithyroid drug 6-propylthiouracil (PTU) was previously shown in our laboratory to have an unexpectedly prolonged inhibitory effect on iodination in the thyroid glands of rats. Eighteen hours after injection of a relatively small dose, iodination in the thyroid remained inhibited by more than 90%. We previously s...

journal_title：Endocrinology

authors： Taurog A,Dorris ML

更新日期：1989-06-01 00:00:00

abstract：:The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...

journal_title：Endocrinology

authors： Otsuka F,Moore RK,Wang X,Sharma S,Miyoshi T,Shimasaki S

更新日期：2005-08-01 00:00:00

abstract：:Inflammatory mediators, such as cytokines and prostaglandins, play a fundamental role in estrogen-dependent breast cancer through their ability to up-regulate aromatase expression and subsequent local production of estrogens in the breast. To study the link between estrogens and inflammation further, we examined the r...

journal_title：Endocrinology

authors： Frasor J,Weaver AE,Pradhan M,Mehta K

更新日期：2008-12-01 00:00:00

abstract：:In previous studies we have shown that aromatase cytochrome P450 (P450arom) mRNA and protein increase markedly in luteal tissue between days 10-19 of gestation, whereas cholesterol side-chain cleavage cytochrome P450 (P450scc) appears to be constitutively maintained regardless of hormonal changes occurring during preg...

journal_title：Endocrinology

authors： Hickey GJ,Oonk RB,Hall PF,Richards JS

更新日期：1989-09-01 00:00:00

abstract：:The nuclear receptor steroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential regulator of endocrine development and function, and the expression of the corresponding gene (sf-1/ad4bp) is precisely regulated in a time- and tissue-dependent manner. We previously demonstrated that the basal promoter...

journal_title：Endocrinology

authors： Hoivik EA,Bjanesoy TE,Mai O,Okamoto S,Minokoshi Y,Shima Y,Morohashi K,Boehm U,Bakke M

更新日期：2011-05-01 00:00:00

abstract：:Insulin-like growth factor (IGF) action in the brain is modulated by IGF-binding proteins (IGFBPs) whose abundance can be altered by other locally expressed growth factors. However, the mechanisms involved are unclear. We here employed the neuroblastoma cell line SK-N-MC as a model to define the mechanisms involved in...

journal_title：Endocrinology

authors： Russo VC,Rekaris G,Baker NL,Bach LA,Werther GA

更新日期：1999-07-01 00:00:00

abstract：:The effect of 3 alpha-hydroxy-4-pregnen-20-one (3HP), a Sertoli cell steroid, on the secretion of gonadotropins from rat anterior pituitary cells in culture was examined and subsequently compared with the action of other gonadal steroids and steroids structurally related to 3HP. Pituitary cells from randomly cycling, ...

journal_title：Endocrinology

authors： Wood PH,Wiebe JP

更新日期：1989-07-01 00:00:00

abstract：:By the use of sucrose density gradient and protamine sulfate analyses, the cytosol fraction of rat lung has been found to contain binding activities for both androgenic and estrogenic steroids. These activities are present in lungs of rats of both sexes and at all ages examined. Both activities interacted stereospecif...

journal_title：Endocrinology

authors： Morishige WK,Uetake CA

更新日期：1978-06-01 00:00:00

abstract：:Decidualization of the endometrial stroma in the rat gives rise to two different cell populations, located either mesometrially or antimesometrially in the uterus. We have previously shown that the rat decidua is the site of production and action of a PRL-like hormone. In this investigation we examined, using reverse ...

journal_title：Endocrinology

authors： Gu Y,Srivastava RK,Clarke DL,Linzer DI,Gibori G

更新日期：1996-11-01 00:00:00

abstract：:Although kisspeptin is the primary stimulator of gonadotropin-releasing hormone secretion and therefore the hypothalamic-pituitary-gonadal axis, recent findings suggest kisspeptin can also regulate additional neuroendocrine processes including release of growth hormone (GH). Here we show that central delivery of kissp...

journal_title：Endocrinology

authors： Foradori CD,Whitlock BK,Daniel JA,Zimmerman AD,Jones MA,Read CC,Steele BP,Smith JT,Clarke IJ,Elsasser TH,Keisler DH,Sartin JL

更新日期：2017-10-01 00:00:00

abstract：:The mechanisms whereby tissue sensitivity to PRL is controlled are not well understood. Here we report that expression of mRNA and protein for members of the SOCS/CIS/JAB family of cytokine signaling inhibitors is increased by PRL administration in ovary and adrenal gland of the lactating rat deprived of circulating P...

journal_title：Endocrinology

authors： Tam SP,Lau P,Djiane J,Hilton DJ,Waters MJ

更新日期：2001-11-01 00:00:00

abstract：:Prostaglandin E2 (PGE2) stimulates the formation of osteoclast-like tartrate-resistant acid phosphatase-positive multinucleated cells (TRAP + MNC) in vitro. This effect likely results from stimulation of adenylyl cyclase, which is mediated by two PGE2 receptors, designated EP2 and EP4. We used cells from mice in which...

journal_title：Endocrinology

authors： Li X,Okada Y,Pilbeam CC,Lorenzo JA,Kennedy CR,Breyer RM,Raisz LG

更新日期：2000-06-01 00:00:00

abstract：:The hypothalamic-pituitary-adrenal axis adapts to periods of chronic or repeated stress. We have now investigated whether there is any differential effect of repeated immobilization stress on CRH and arginine vasopressin (AVP) gene transcription in the hypothalamic paraventricular nucleus (PVN), using probes for both ...

journal_title：Endocrinology

authors： Ma XM,Levy A,Lightman SL

更新日期：1997-10-01 00:00:00

abstract：:The study of epigenetics allows for the understanding of gene × environmental interactions and provides a mechanism by which brief internal or external environmental changes can shape lasting differences in gene function and behavior. Epigenetic processes appear to impact a wide variety of physiological processes with...

journal_title：Endocrinology

authors： Auger AP,Auger CJ

更新日期：2011-02-01 00:00:00

abstract：:Continuous labeling of primary cultures of human syncytiotrophoblasts with [125I]iodoepidermal growth factor (iodo-EGF) at 37 C revealed a progressive increase in intracellular EGF with 5% of total label observed in intracellular organelles at 2 min and 88% by 60 min. Detailed analysis by electron microscope radioauto...

journal_title：Endocrinology

authors： Lai WH,Guyda HJ,Bergeron JJ

更新日期：1986-01-01 00:00:00

abstract：:IGF-II has been reported to decrease neonatal islet cell apoptosis and in vitro adult islet cell necrosis and apoptosis, but the usefulness of IGF-II in a transplantation setting is unknown. We evaluated the effect of in vitro IGF-II incubations on microencapsulated rat islet survival both in vitro and in minimal mass...

journal_title：Endocrinology

authors： Robitaille R,Dusseault J,Henley N,Rosenberg L,Hallé JP

更新日期：2003-07-01 00:00:00

abstract：:Neonatal thymectomy at 3 days of age in mice results in ovarian dysgenesis, characterized by early follicular and oocyte loss, followed by a high incidence of ovarian tumorigenesis. Thymectomized animals with dysgenetic ovaries had extremely high levels of circulating PRL and gonadotropins before the appearance of the...

journal_title：Endocrinology

authors： Michael SD,Taguchi O,Nishizuka Y

更新日期：1981-06-01 00:00:00

abstract：:Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals ...

journal_title：Endocrinology

authors： Schroeder MD,Brockman JL,Walker AM,Schuler LA

更新日期：2003-12-01 00:00:00

abstract：:Some 17 alpha-alkylated androgens used as anabolic agents, such as stanozolol (ST) and danazol (DA), have specific effects on the liver that are not exerted by testosterone. This gives rise to the possibility that a steroid-binding protein, other than the androgen receptor, could modulate the intracellular actions of ...

journal_title：Endocrinology

authors： Fernández L,Chirino R,Boada LD,Navarro D,Cabrera N,del Rio I,Díaz-Chico BN

更新日期：1994-03-01 00:00:00

abstract：:LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...

journal_title：Endocrinology

authors： Bassetti M,Pasini DL,Rosa P

更新日期：1995-03-01 00:00:00