Abstract:
:Cysteamine [2-aminoethanethiol (CySH)] reduces measurable PRL concentrations in vivo and in vitro. Since secretion is also inhibited, CySH may block conversion from a poorly assayable hormone storage form(s) to readily assayable, releasable PRL. This would represent a previously unrecognized mechanism for secretory regulation. We undertook the present study to identify the sites involved in the loss of measurable PRL (depletion) induced by cysteamine. The disulfide cystamine was ineffective on secretory granules unless combined with reduced glutathione, indicating the generation of the active CySH-thiol form. Pretreatment of granules with thiol-blocking agents resulted in dose-dependent enhancement of CySH inhibition, achieving nearly complete inhibition with 5 mM iodoacetamide. In contrast, pretreatment with reduced glutathione or dithiothreitol, respectively, impaired or abolished the CySH effect. Similar results were obtained when tissues were exposed to thiols and/or alkaline conditions after CySH treatment; for example, exposure to 50 mM glutathione reduced the lowering of measured pituitary homogenate PRL from 76% to 37%. These data suggest that the mechanism by which CySH causes PRL depletion is mediated by granule disulfides and the -SH of CySH. The regulation of thiol:disulfide equilibria appears to be an important determinant of the detectability of PRL storage forms and of their secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lorenson MY,Jacobs LSdoi
10.1210/endo-115-4-1492subject
Has Abstractpub_date
1984-10-01 00:00:00pages
1492-5issue
4eissn
0013-7227issn
1945-7170journal_volume
115pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The adrenal cortex contains a kallikrein-like enzyme that may lead to bradykinin (BK) formation. This study was designed to determine whether BK acts on adrenocortical cells to stimulate steroid secretion. BK, Lys-BK, a specific BK 2 (B2) receptor agonist, and desArg9-BK, a specific BK 1 (B1) receptor agonist, all sti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1312440
更新日期:1992-04-01 00:00:00
abstract::In fish spermatogenesis, the main action of progestins is generally regarded as the induction of sperm maturation. Our previous in vitro study demonstrated that a progestin, 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), induced the initiation of meiosis in spermatogenesis in the Japanese eel (Anguilla japonica). In ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0391
更新日期:2006-11-01 00:00:00
abstract::Diabetics have at least twice the risk of stroke and may show performance deficit in a wide range of cognitive domains. The mechanisms underlying this gradually developing end-organ damage may involve both vascular changes and direct damage to neuronal cells as a result of overproduction of superoxide by the respirato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0712
更新日期:2005-12-01 00:00:00
abstract::The mammalian suprachiasmatic nucleus is the site of the circadian rhythm generator. The degrees of expression there of several neuropeptides, including vasopressin and vasoactive intestinal polypeptide, follow a diurnal rhythm. The vasopressin V1a receptor, whose activation results in phosphoinositol hydrolysis and m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8344200
更新日期:1993-08-01 00:00:00
abstract::Previous studies from our laboratory have shown that messenger RNAs (mRNAs) coding for a cAMP-specific phosphodiesterase (PDE4A) are present in mature rat and mouse germ cells. However, no information is available about the properties of the expressed proteins. To determine their structure and regulation, the PDE4A is...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641200
更新日期:1996-06-01 00:00:00
abstract::Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0826
更新日期:2003-12-01 00:00:00
abstract::Limited proteolysis in vivo of insulin-like growth factor-binding protein-3 (IGFBP-3) by as yet unidentified serine proteases plays a key role in controlling the bioavailability of IGFBP-3-associated insulin-like growth factors (IGFs). Both the IGF system and the system of plasminogen activators (PAs) and their inhibi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7527330
更新日期:1994-12-01 00:00:00
abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588270
更新日期:1995-12-01 00:00:00
abstract::Posttranslational processing of proadrenomedullin generates two biologically active peptides, adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP). Sequence comparison of homologous proadrenomedullin genes in vertebrate evolution shows a high degree of stability in the reading frame for AM, whereas P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0105
更新日期:2006-07-01 00:00:00
abstract::The regulation of cholecystokinin octapeptide (CCK-8) secretion was studied using a fetal cerebral cortical cell culture system. After 2-3 weeks in culture, the cells were utilized for short-term release experiments. CCK-8 was measured by RIA and its identity was confirmed by HPLC. Depolarization of the cells with K+ ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3125
更新日期:1991-12-01 00:00:00
abstract::A midregion fragment of PTH-related protein (PTHrP), which is intensively conserved across species, has been identified as a secretory product of several different cell types, including keratinocytes and squamous carcinomas. As recent data suggest that a midregion PTHrP fragment may be biologically active, we hypothes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940360
更新日期:1996-12-01 00:00:00
abstract::Kisspeptin-neurokinin B-dynorphin (KNDy) cells of the hypothalamus are a key component in the neuroendocrine regulation of GnRH secretion. Evidence in sheep and other species suggests that dynorphin released by KNDy cells inhibits pulsatile GnRH secretion by acting upon κ-opioid receptors (KOR). However, the precise a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1763
更新日期:2016-06-01 00:00:00
abstract::Hypothalamic enzymatic activities capable of degrading LHRH may play a physiological role in the neuroendocrine control of LHRH. There is increasing evidence, however, that these enzymes are not peptide specific. The present study in the ewe analyzed the possibility that the specificity of LHRH-degrading activity (LHR...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2410
更新日期:1985-06-01 00:00:00
abstract::In adult male rhesus monkeys, 1 day of fasting leads to a profound suppression of LH secretion that is rapidly reversed with refeeding. To test the hypothesis that changes in insulin secretion during fasting and refeeding mediate the changes in LH secretion, blood samples for LH measurement were obtained between 0900-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641210
更新日期:1996-06-01 00:00:00
abstract::To study blood capillary growth in the hyperplastic thyroid gland, rats were fed 0.25% thiouracil in a low iodine diet for time intervals up to 100 days. Thyroids were fixed by vascular perfusion and embeded in Epon. Whole lobes were sectioned from pole to pole and slides were prepared of sections every 0.3 mm. Capill...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2306
更新日期:1978-12-01 00:00:00
abstract::The mechanism of the inhibitory effect of long term treatment with estradiol on dopamine synthesis in tuberoinfundibular dopaminergic (TIDA) neurons was studied by using hypothalamic slices from ovariectomized rats. Treatment with 2 mg estradiol valerate (EV) at a 3-week interval increased the weight of the anterior p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-692
更新日期:1987-08-01 00:00:00
abstract::Preincubation of rat pituitary cells in primary culture with rat GH-releasing factor (rGRF) resulted in substantial desensitization to subsequent GRF stimulation. rGRF-directed GH release and intracellular cAMP accumulation decreased in the desensitized cells. Whereas prior treatment of rat pituitary cells caused part...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1334
更新日期:1985-04-01 00:00:00
abstract::The original objective of this study was to use available distribution volume (V) and deca constant (k) estimates for rat LH and FSH to determine the amounts of these hormones synthesized and released during a dynamic secretory event, the postpartum preovulatory surge. The problem was approached by 1) determining the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-3-665
更新日期:1978-09-01 00:00:00
abstract::Circulating levels of calcitonin precursors (CTpr), including procalcitonin (ProCT), increase up to several thousand-fold in human sepsis, and immunoneutralization improves survival in two animal models of this disease. Herein, we analyzed inflammation-mediated calcitonin I gene (CALC I) expression in human adipocyte ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0854
更新日期:2003-12-01 00:00:00
abstract::Up-regulation of maternal islet function is essential to accommodate the increased demand for insulin during pregnancy. Previously, we suggested that lactogenic activity regulates islet function during pregnancy. However, this hypothesis was based on the effect of homologous PRLs on islets, since the homologous placen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425500
更新日期:1993-02-01 00:00:00
abstract::A functioning rat thyroid cell line (FRTL-5) responds acutely to the addition of TSH, norepinephrine, and the Ca2+ ionophore A23187 by a depression in iodide (I-) uptake levels. The decrease in I- content measured at the steady state depends on the presence of external Ca2+ and can be accounted for by an effect on sti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1108
更新日期:1984-04-01 00:00:00
abstract::These studies have examined the ontogeny of specific GH- and PRL-binding proteins in rabbit liver cytosol across the fetal, early neonatal, and adult periods. The precise hormonal specificity of binding of the somatotropic/lactogenic ligand, [125I]human GH, was determined by displacement with monoclonal antibodies aga...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-516
更新日期:1989-07-01 00:00:00
abstract::The effects of insulin to stimulate metabolic and mitogenic responses were examined in Rat 1 fibroblast cells that overexpressed either normal (HIRc) or kinase-deficient human insulin receptors. When studied at the optimal growth stage for each cell line, insulin-stimulated responses measured in cells containing kinas...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7534700
更新日期:1995-04-01 00:00:00
abstract::The expression of the microsomal (M) antigen on the surface and in the cytoplasm of a strain of rat thyroid cells (FRTL-5) is under the regulation of TSH. In the present report the mechanism by which TSH induces such expression was investigated with the use of human microsomal antibody-positive serum and an indirect i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-2-1140
更新日期:1988-08-01 00:00:00
abstract::Cultured pineal ependymal cells from rat fetuses aged 17 to 19 days post-coitum release into their media a substance that has hydroosmotic, antidiuretic and rat uterine activities. Paper chromatography of concentrates from culture media demonstrates that the substance possessing all the above activities was eluted fro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-1-205
更新日期:1977-01-01 00:00:00
abstract::The nonpeptide CRH antagonist antalarmin has been shown to block both behavioral and endocrine responses to CRH. However, it's potential activity in blunting behavioral and endocrine sequelae of stressor exposure has not been assessed. Because antagonism of central CRH by alpha-helical CRH attenuates conditioned fear ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6415
更新日期:1999-01-01 00:00:00
abstract::This study was designed to test the hypothesis that systemic immune challenge with endotoxin inhibits the reproductive axis centrally by suppressing GnRH pulsatile release into hypophyseal portal blood. Using alert, normally behaving, ovariectomized ewes, we sampled hypophyseal portal blood at 10-min intervals beginni...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5449
更新日期:1997-10-01 00:00:00
abstract::Sex differences in vocal communication are prevalent in both the animals and humans. The mechanism(s) mediating gender differences in human language are unknown, although, sex hormones, principally androgens, play a central role in the development of vocalizations in a wide variety of animal species. The discovery of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1486
更新日期:2014-12-01 00:00:00
abstract::The development of androgen responsiveness in the submandibular gland of normal and androgen-resistant (Tfm) mice of different ages was studied after varying hormonal treatments. Total esteroproteolytic (tamase) activity of submandibular gland homogenates was used as a marker for androgen action. Newborn mice of all f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1571
更新日期:1975-06-01 00:00:00
abstract::The metabolic consequences of visceral obesity have been associated with amplification of glucocorticoid action by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in adipose tissue. This study aimed to assess in a rat model of diet-induced obesity the effects of pharmacological 11beta-HSD1 inhibition on the m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1199
更新日期:2007-05-01 00:00:00