Multiple forms of affinity-labeled estrogen receptors in rat distinct pituitary cells.

abstract：:Dopamine D2 receptor agonists are commonly used in the control of PRL-secreting adenomas, and the sensitivity of dopamine agonists during long term therapy is exquisite. However, the molecular mechanisms responsible for the maintenance of this cellular sensitivity to dopamine agonists remain poorly understood. In the ...

journal_title：Endocrinology

authors： Ng GY,Varghese G,Chung HT,Trogadis J,Seeman P,O'Dowd BF,George SR

更新日期：1997-10-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF) blocks LH-stimulated androstenedione production by immature rat theca-interstitial cells (TIC) in vitro. The mechanism for TNF inhibition of LH-induced androstenedione is unknown and was investigated. LH stimulation of androstenedione synthesis in TIC is mediated via a cAMP-dependent ...

journal_title：Endocrinology

authors： Zachow RJ,Tash JS,Terranova PF

更新日期：1993-11-01 00:00:00

abstract：:Bone morphogenetic protein (BMP)-4 is a key mediator of anterior pituitary organogenesis. However, through inappropriate expression patterns, BMP-4 is also pathogenic in a pituitary adenoma subtype-specific context. In these cases, increase or decrease in BMP-4 in lactotroph- and corticotroph-derived adenomas, respect...

journal_title：Endocrinology

authors： Yacqub-Usman K,Duong CV,Clayton RN,Farrell WE

更新日期：2012-08-01 00:00:00

abstract：:Reports of estrone (E1) and dehydroepiandrosterone (DHEA) sulfatase (sulfohydrolase) activities within many human breast cancers have prompted us to undertake the identification and partial characterization of these enzyme activities within MCF-7 human breast cancer cells. Enzyme assays were performed within subcellul...

journal_title：Endocrinology

authors： MacIndoe JH

更新日期：1988-09-01 00:00:00

abstract：:Because there are sex differences in feeding behavior in rats, we looked for a possible sex difference in the response to glucose of melanin-concentrating hormone (MCH) neurons in the lateral hypothalamic area using phosphorylated cAMP response element-binding protein (pCREB) as a marker of neural activity. Intact mal...

journal_title：Endocrinology

authors： Mogi K,Funabashi T,Mitsushima D,Hagiwara H,Kimura F

更新日期：2005-08-01 00:00:00

abstract：:Historically, much of the research on health effects of environmental pollutants focused on ascertaining whether compounds were carcinogenic. More recent findings show that environmental contaminants also exert insidious effects by disrupting hormone action. Of particular concern are findings that developmental exposu...

journal_title：Endocrinology

authors： Petersen SL,Krishnan S,Hudgens ED

更新日期：2006-06-01 00:00:00

abstract：:Modulation of activin and other TGF beta superfamily signaling is the primary mechanism of action for both follistatin (FS) and FS-like 3 (FSTL-3). However, most studies of these ligands use activin A due to its wide availability. We have now tested the ability of FS288 and FSTL-3 to bind and neutralize activin B rela...

journal_title：Endocrinology

authors： Schneyer A,Schoen A,Quigg A,Sidis Y

更新日期：2003-05-01 00:00:00

abstract：:The technologies for pancreatic β-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH mini...

journal_title：Endocrinology

authors： Cheng Y,Su Y,Shan A,Jiang X,Ma Q,Wang W,Ning G,Cao Y

更新日期：2015-07-01 00:00:00

abstract：:Although it is evident that androgens increase muscle mass and strength, little is known about the critical molecular targets of androgens in skeletal muscle. In rodents, the skeletal alpha-actin gene is a tissue-specific gene expressed only in the levator ani and other skeletal muscles but not in the prostate or prep...

journal_title：Endocrinology

authors： Hong MH,Sun H,Jin CH,Chapman M,Hu J,Chang W,Burnett K,Rosen J,Negro-Vilar A,Miner JN

更新日期：2008-03-01 00:00:00

abstract：:Mice lacking steroid 5 alpha-reductase 1 and 2 were produced by gene targeting and breeding. Male mice without 5 alpha-reductase 2 or without both enzymes had fully formed internal and external genitalia and were fertile, but had smaller prostates and seminal vesicles than controls. T accumulated to high levels in the...

journal_title：Endocrinology

authors： Mahendroo MS,Cala KM,Hess DL,Russell DW

更新日期：2001-11-01 00:00:00

abstract：:Acute effects of bisphenol (BPA), an environmental chemical, on estradiol (17α or β-E2)-dependent recognition memory and dendritic spines in the medial prefrontal cortex and hippocampus were investigated in adult female rats. Ovariectomized rats received BPA 30 min before or immediately after a sample trial (viewing o...

journal_title：Endocrinology

authors： Inagaki T,Frankfurt M,Luine V

更新日期：2012-07-01 00:00:00

abstract：:[125I]-[D-Ser(TBU)6]LHRH-EA binds to a single class of high affinity sites in rat anterior pituitary cells in culture at an apparent dissociation constant of 0.25 nM at 0-4C. The order of potency of a representative group of LHRH agonists and antagonists to displace the labeled ligand is similar to their LH-releasing ...

journal_title：Endocrinology

authors： Giguere V,Lefebvre FA,Labrie F

更新日期：1981-01-01 00:00:00

abstract：:The effect of osmotic changes on aldosterone production, [Ca2+]i and voltage-gated Ca2+ currents, was studied in cultured rat glomerulosa cells. Alteration of osmolarity by sucrose addition in the 250-330 mosM range did not influence aldosterone production per se, but it substantially affected K+-stimulated aldosteron...

journal_title：Endocrinology

authors： Makara JK,Petheö GL,Tóth A,Spät A

更新日期：2000-05-01 00:00:00

abstract：:Dispersed cells from whole testes or from isolated interstitial tissue of mature rats, yielded two distinct populations of Leydig cells when subjected to centrifugation in a 0-40% metrizamide gradient. One population (I) was found in a fractions with a density of 1.085-1.117 g/cm3, and the other population (II) was fo...

journal_title：Endocrinology

authors： Payne AH,Downing JR,Wong KL

更新日期：1980-05-01 00:00:00

abstract：:The effect of synthetic ovine CRF on ACTH secretion of dispersed, domestic fowl pituitary cells was investigated. Cells preincubated for 2 h, 16 h, or after 48-h culture were incubated briefly with CRF (up to 4 h). ACTH was bioassayed using isolated rat adrenocortical cells; ACTH-(1-24) served as the standard for expr...

journal_title：Endocrinology

authors： Carsia RV,Weber H,Perez FM Jr

更新日期：1986-01-01 00:00:00

abstract：:In amphibians, the secretion of alpha-MSH by melanotrope cells is stimulated by TRH and inhibited by NPY. We have previously shown that NPY abrogates the stimulatory effect of TRH on alpha-MSH secretion. The aim of the present study was to characterize the receptor subtypes mediating the action of NPY and to investiga...

journal_title：Endocrinology

authors： Galas L,Tonon MC,Beaujean D,Fredriksson R,Larhammar D,Lihrmann I,Jegou S,Fournier A,Chartrel N,Vaudry H

更新日期：2002-05-01 00:00:00

abstract：:The Rice H500 rat Leydig cell tumor is a well characterized model of humoral hypercalcemia of malignancy (HHM). Circulating concentrations of PTH-related protein (PTHRP) have not been reported in this or any other animal model of HHM. Taking advantage of the marked N-terminal amino acid homology between rodent and hum...

journal_title：Endocrinology

authors： Gaich G,Burtis WJ

更新日期：1990-09-01 00:00:00

abstract：:Proper development of the seminiferous tubules (or testis cords in embryos) is critical for male fertility. Sertoli cells, somatic components of the seminiferous tubules, serve as nurse cells to the male germline, and thus their numbers decide the quantity of sperm output in adulthood. We previously identified activin...

journal_title：Endocrinology

authors： Archambeault DR,Tomaszewski J,Childs AJ,Anderson RA,Yao HH

更新日期：2011-11-01 00:00:00

abstract：:An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77,000, its N-terminal amino acid sequence is the same as prothrombin's for 15 residues, it contains gamma-carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-rea...

journal_title：Endocrinology

authors： Henrikson KP,Jazin EE,Greenwood JA,Dickerman HW

更新日期：1990-01-01 00:00:00

abstract：:The electrical responses of rat islet cells maintained in culture with varying levels of glucose were determined. After culture with 2.8 or 4.2 mM glucose, the beta-cells did not respond electrically to 27.8 mM glucose. However, after culture in 5.6 mM glucose, the cells responded to 27.8 mM glucose in short term expe...

journal_title：Endocrinology

authors： Pace CS,Conant S,Lacy PE

更新日期：1978-05-01 00:00:00

abstract：:The levels of the GnRH receptor (GnRHR) and its messenger RNA depend on the pattern of administration of GnRH. In this study, internal deletion mutants in a luciferase reporter gene vector (GnRHR-pXP2) containing a 1226-bp promoter fragment of mouse GnRHR gene were used to examine the regulation of GnRHR gene transcri...

journal_title：Endocrinology

authors： Maya-Núñez G,Conn PM

更新日期：1999-08-01 00:00:00

abstract：:Acylation-stimulating protein (ASP) is a potent lipogenic protein produced by adipocytes. In vitro studies have shown that ASP increases triglyceride synthesis and glucose transport in both murine and human adipocytes. Our initial study indicated that complement C3-deficient (-/-) mice (and, therefore, ASP deficient) ...

journal_title：Endocrinology

authors： Murray I,Havel PJ,Sniderman AD,Cianflone K

更新日期：2000-03-01 00:00:00

abstract：:alpha-Fetoprotein (AFP) is present in high levels in fetal fluids, certain neoplasias, and regenerating liver. Although AFP's physiological role remains an enigma, we have recently demonstrated mitogenic activity for AFP. Using a primary monolayer culture system, we have further investigated the proliferative activity...

journal_title：Endocrinology

authors： Keel BA,Eddy KB,Cho S,May JV

更新日期：1991-07-01 00:00:00

abstract：:The multifunctional cytokine, transforming growth factor-beta (TGF beta), is found in many tissues in a latent or inactive form. The nature and composition of the latent complex can vary depending on tissue type. The release of active TGF beta from its latent complex is a potentially important mechanism for regulation...

journal_title：Endocrinology

authors： Bonewald LF,Oreffo RO,Lee CH,Park-Snyder S,Twardzik D,Mundy GR

更新日期：1997-02-01 00:00:00

abstract：:In an effort to define biochemical events relevant to the adipogenic action of GH, the effect of GH on expression of the cytoskeletal element vinculin was studied in 3T3-F442A preadipose cells. Results from Western blotting indicated that in serum-free medium 2 nM met-hGH induced an approximately 100% increase in vinc...

journal_title：Endocrinology

authors： Guller S,Corin RE,Yuan-Wu K,Sonenberg M

更新日期：1991-07-01 00:00:00

abstract：:Insulin-like growth factor (IGF) I, a polypeptide synthesized by skeletal cells, and its amino terminus truncated derivative desamino-(1-3)-IGF I (des-IGF I) were compared for their effects on bone formation in vitro. Des-IGF I and IGF I were studied for their effects on DNA and collagen synthesis in cultures of intac...

journal_title：Endocrinology

authors： Canalis E,McCarthy TL,Centrella M

更新日期：1991-07-01 00:00:00

abstract：:The effects of retinoic acids (RAs) on development of seminal vesicles (SVs) of neonatal mice were investigated in vitro. SVs from 0-day-old male mice were cultured for 2-6 days in serum-free, chemically defined medium containing transferrin and BSA supplemented with 5alpha-dihydrotestosterone (DHT; 10(-8) M) and insu...

journal_title：Endocrinology

authors： Tanji N,Yokoyama M,Takeuchi M,Terada N,Dahiya R,Cunha GR

更新日期：1996-07-01 00:00:00

abstract：:In the present study, we report that ACTH induces a transient chloride current. The lack of correlation between ACTH-induced cAMP production and amplitude of the Cl- current, as well as the absence of stimulation by forskolin or 8Br-cAMP indicated that the ACTH-induced current was not cAMP-dependent. We explored the p...

journal_title：Endocrinology

authors： Chorvátová A,Gendron L,Bilodeau L,Gallo-Payet N,Payet MD

更新日期：2000-02-01 00:00:00

abstract：:Specific retinoid X receptor (RXR) agonists, such as LG100268 (LG268), and the thiazolidinedione (TZD) PPARgamma agonists, such as rosiglitazone, produce insulin sensitization in rodent models of insulin resistance and type 2 diabetes. In sharp contrast to the TZDs that produce significant increases in body weight gai...

journal_title：Endocrinology

authors： Leibowitz MD,Ardecky RJ,Boehm MF,Broderick CL,Carfagna MA,Crombie DL,D'Arrigo J,Etgen GJ,Faul MM,Grese TA,Havel H,Hein NI,Heyman RA,Jolley D,Klausing K,Liu S,Mais DE,Mapes CM,Marschke KB,Michellys PY,Montrose-Rafi

更新日期：2006-02-01 00:00:00

abstract：:Pituitary homeobox 1 (Ptx1) is a homeodomain-containing transcription factor acting on transcription of all pituitary hormone genes. Its expression is first detected in the stomodeal ectoderm and is maintained in all derivatives of this structure, including Rathke's pouch. We now show that Ptx1 is expressed in all pit...

journal_title：Endocrinology

authors： Lanctôt C,Gauthier Y,Drouin J

更新日期：1999-03-01 00:00:00