Abstract:
:The technologies for pancreatic β-cell-specific gene overexpression or knockout are fundamental for investigations of functional genes in vivo. Here we generated the Ins1-Cre-Dsred and Ins1-rtTA mouse models, which expressed the Cre recombinase or reverse tetracycline regulatable transactivator (rtTA) without hGH minigene under the control of mouse Ins1 promoter. Our data showed that the Cre-mediated recombination and rtTA-mediated activation could be efficiently detected at embryonic day 13.5 when these models were crossed with the reporter mice (ROSA(mT/mG) or tetO-HIST1H2BJ/GFP). The Cre and rtTA expression was restricted to β-cells without leakage in the brain and other tissues. Moreover, both the transgenic lines showed normal glucose tolerance and insulin secretion. These results suggested that the Ins1-Cre-Dsred and Ins1-rtTA mice could be used to knock out or overexpress target genes in embryos and adults to facilitate β-cell researches.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Cheng Y,Su Y,Shan A,Jiang X,Ma Q,Wang W,Ning G,Cao Ydoi
10.1210/en.2015-1104subject
Has Abstractpub_date
2015-07-01 00:00:00pages
2724-31issue
7eissn
0013-7227issn
1945-7170journal_volume
156pub_type
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