Dexamethasone-induced changes in phosphorylation of the insulin and epidermal growth factor receptors and their substrates in intact rat hepatocytes.


:Dexamethasone-induced changes in insulin and epidermal growth factor (EGF) receptor number, autophosphorylation, and kinase activity were studied in intact rat hepatocytes. Hepatocytes were freshly isolated from Sprague-Dawley rats treated with dexamethasone (1 mg/kg) for 4 days and from untreated littermates. Dexamethasone had no effect on insulin receptor number, while EGF receptor binding was slightly increased (21.3% vs. 17.2% binding/10(6) cells) after dexamethasone treatment. In hepatocytes from both control and dexamethasone-treated animals labeled with 32P, insulin induced tyrosine phosphorylation of the beta-subunit of the insulin receptor as well as of a 175K protein believed to be its endogenous substrate. The degree of phosphorylation of the insulin receptor was decreased 34% by dexamethasone treatment compared to the control value when studied in fasted animals. In contrast, phosphorylation was increased to a similar extent by dexamethasone treatment in fed animals. In addition, the beta-subunit of the insulin receptor extracted from dexamethasone-treated animals migrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a slightly increased mobility compared to normal (89 +/- 1.2K vs. 92.5 +/- 0.4K). EGF induced tyrosine phosphorylation of its own receptor and of a 120K protein in intact hepatocytes. Their degree of phosphorylation was decreased by 30% as a result of dexamethasone treatment in the fasted animal and was unchanged in the fed animals. Our data indicate that glucocorticoids modulate insulin and EGF receptor kinase activity, but the nature of their effect depends on other factors, including the dietary state of the animal. These studies also suggest that postreceptor changes account for a major component of glucocorticoid-induced insulin resistance.






Karasik A,Kahn CR




Has Abstract


1988-11-01 00:00:00












  • Detection of placental growth hormone variant and chorionic somatomammotropin ribonucleic acid expression in human trophoblastic neoplasms by reverse transcriptase-polymerase chain reaction.

    abstract::Attempts to assess human placental GH variant (hGH-V) and chorionic somatomammotropin (hCS) RNA in choriocarcinoma cell lines have been hampered by low levels of expression and limited sensitivity of RNA blotting analysis. We examined human choriocarcinoma BeWo, JAR, and JEG-3 cell lines as well as samples of complete...


    pub_type: 杂志文章


    authors: Lytras A,Bock ME,Dodd JG,Cattini PA

    更新日期:1994-06-01 00:00:00

  • Effects of indoleamines and their newly identified metabolites on prolactin release in rats.

    abstract::Plasma immunoreactive PRL responses to indoleamines and their metabolites were studied in urethane-anesthetized rats. All drugs were injected into the lateral ventricle and blood samples were serially collected from a jugular vein. Serotonin and melatonin caused a significant increase in plasma PRL with peak values at...


    pub_type: 杂志文章


    authors: Iwasaki Y,Kato Y,Ohgo S,Abe H,Imura H,Hirata F,Senoh S,Tokuyama T,Hayaishi O

    更新日期:1978-07-01 00:00:00

  • Inhibition of the enzymatic degradation of Met-enkephalin by catecholamines.

    abstract::The influence of the catecholamine hormones and some of their metabolites on the enzymatic degradation of met-enkephalin by rat brain homogenates has been evaluated. The enzyme activity was determined by the release of [125I]-tyrosine from radioiodinated met-enkephalin following Porapak Q column chromatography. Each o...


    pub_type: 杂志文章


    authors: Caffrey JL,Hodges DH

    更新日期:1982-01-01 00:00:00

  • Estrogen-induced calbindin-D 9k gene expression in the rat uterus during the estrous cycle: late antagonistic effect of progesterone.

    abstract::Progesterone modulates estrogen-stimulated responses in the uterus. Calbindin-D 9k (CaBP9k), a 17 beta-estradiol-responsive gene expressed in the uterus, was used as a marker to examine the interactions between endogenous progesterone and estradiol in the rat. The variations in uterine CaBP9k messenger RNAs (mRNAs) du...


    pub_type: 杂志文章


    authors: L'Horset F,Blin C,Brehier A,Thomasset M,Perret C

    更新日期:1993-02-01 00:00:00

  • Two molecular forms of gonadotropin-releasing hormone from the brain of the frog, Rana ribibunda: purification, characterization, and distribution.

    abstract::The molecular forms of GnRH in amphibia have not been characterized structurally. An antiserum to mammalian GnRH that shows appreciable cross-reactivity with chicken GnRH-I ([Gln8]GnRH), chicken GnRH-II ([His5,Trp7,Tyr8]GnRH), and salmon GnRH ([Trp7, Leu8]GnRH) was used in a RIA to detect GnRH-related peptides in an e...


    pub_type: 杂志文章


    authors: Conlon JM,Collin F,Chiang YC,Sower SA,Vaudry H

    更新日期:1993-05-01 00:00:00

  • Pegylated leptin antagonist is a potent orexigenic agent: preparation and mechanism of activity.

    abstract::Leptin, a pleiotropic adipokine, is a central regulator of appetite and weight and a key immunomodulatory protein. Although inborn leptin deficiency causes weight gain, it is unclear whether induced leptin deficiency in adult wild-type animals would be orexigenic. Previous work with a potent competitive leptin antagon...


    pub_type: 杂志文章


    authors: Elinav E,Niv-Spector L,Katz M,Price TO,Ali M,Yacobovitz M,Solomon G,Reicher S,Lynch JL,Halpern Z,Banks WA,Gertler A

    更新日期:2009-07-01 00:00:00

  • Acute stress differentially affects aromatase activity in specific brain nuclei of adult male and female quail.

    abstract::The rapid and temporary suppression of reproductive behavior is often assumed to be an important feature of the adaptive acute stress response. However, how this suppression operates at the mechanistic level is poorly understood. The enzyme aromatase converts testosterone to estradiol in the brain to activate reproduc...


    pub_type: 杂志文章


    authors: Dickens MJ,Cornil CA,Balthazart J

    更新日期:2011-11-01 00:00:00

  • Thyrotropin regulates thyrotroph responsiveness to dopamine in vitro.

    abstract::The effect of conditioned vs. fresh culture medium on the dopaminergic inhibition of TSH and PRL secretion by primary cultures of male rat anterior pituitary cells has been studied. In the presence of conditioned medium (that had been in contact with the cells over the 3-day culture period) 10(-6) M dopamine (DA) inhi...


    pub_type: 杂志文章


    authors: Foord SM,Peters JR,Dieguez C,Shewring G,Hall R,Scanlon MF

    更新日期:1986-04-01 00:00:00

  • Minireview: A hypothalamic role in energy balance with special emphasis on leptin.

    abstract::The hypothalamus is a major site for integration of central and peripheral signals that regulate energy homeostasis. Within the hypothalamus, neurons residing in the ARC (arcuate nucleus)-PVN (paraventricular)-PF/LH (perifornical/lateral hypothalamus) axis communicate among each other and are subjected to the influenc...


    pub_type: 杂志文章,评审


    authors: Sahu A

    更新日期:2004-06-01 00:00:00

  • Prenatal Dexamethasone Exposure Induced Ovarian Developmental Toxicity and Transgenerational Effect in Rat Offspring.

    abstract::Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian developmental toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d dexamethasone from ge...


    pub_type: 杂志文章


    authors: Lv F,Wan Y,Chen Y,Pei L,Luo D,Fan G,Luo M,Xu D,Wang H

    更新日期:2018-03-01 00:00:00

  • Growth hormone (GH) binding protein and GH interactions in vivo in the guinea pig.

    abstract::GH binding proteins (GHBPs) are present in the blood of several species and by complexing with circulating GH may alter its clearance and distribution. The actions of human GHBP (hGHBP) have hitherto been studied indirectly via their effects on the clearance of hGH. In the present experiments, recombinant preparations...


    pub_type: 杂志文章


    authors: Fairhall KM,Carmignac DF,Robinson IC

    更新日期:1992-10-01 00:00:00

  • Effect of bisphosphonates on the rapidly growing male murine skeleton.

    abstract::Bisphosphonates are effective for preventing and treating skeletal disorders associated with hyperresorption. Their safety and efficacy has been studied in adults where the growth plate is fused and there is no longitudinal bone growth and little appositional growth. Although bisphosphonate use in the pediatric popula...


    pub_type: 杂志文章


    authors: Zhu ED,Louis L,Brooks DJ,Bouxsein ML,Demay MB

    更新日期:2014-04-01 00:00:00

  • Effect of recombinant activin-A on gonadotropin secretion in the female rat.

    abstract::Repeated injections of recombinant human (rh) activin-A over a 2- to 3-day period are reportedly needed to stimulate the in vivo secretion of FSH. In this paper we present results showing that acute treatment with rh-activin-A caused marked and dose-dependent increases in plasma FSH, but not LH, levels in adult female...


    pub_type: 杂志文章


    authors: Rivier C,Vale W

    更新日期:1991-11-01 00:00:00

  • Parathyroid hormone-related protein and interleukin-1 alpha synergistically stimulate bone resorption in vitro and increase the serum calcium concentration in mice in vivo.

    abstract::To elucidate the mechanism of humoral hypercalcemia elicited by human esophageal carcinoma cells (EC-GI), which constitutively produced interleukin-1 alpha (IL-1 alpha) and PTH-like factor, the effects of IL-1 alpha and PTH-related protein (PTH-rP) on bone resorption in vitro and on serum calcium concentrations in viv...


    pub_type: 杂志文章


    authors: Sato K,Fujii Y,Kasono K,Ozawa M,Imamura H,Kanaji Y,Kurosawa H,Tsushima T,Shizume K

    更新日期:1989-05-01 00:00:00

  • Synthetic peptides corresponding to human follicle-stimulating hormone (hFSH)-beta-(1-15) and hFSH-beta-(51-65) induce uptake of 45Ca++ by liposomes: evidence for calcium-conducting transmembrane channel formation.

    abstract::We have previously described FSH receptor-mediated influx of 45Ca++ in cultured Sertoli cells from immature rats and receptor-enriched proteoliposomes via activation of voltage-sensitive and voltage-independent calcium channels. We have further shown that this effect of FSH does not require cholera toxin- or pertussis...


    pub_type: 杂志文章


    authors: Grasso P,Santa-Coloma TA,Reichert LE Jr

    更新日期:1991-06-01 00:00:00

  • Failure of growth hormone (GH) to feed back at the level of the pituitary to alter the response of the somatotrophs to GH-releasing factor.

    abstract::GH feeds back at the level of the central nervous system to alter the release of somatostatin and GRF, resulting in altered GH release. The purpose of this study was to see whether the concentration of GH, impinging directly on the somatotrophs of the adenohypophysis, would alter the responsiveness of the somatotrophs...


    pub_type: 杂志文章


    authors: Kraicer J,Lussier B,Moor BC,Cowan JS

    更新日期:1988-04-01 00:00:00

  • Decreased thyroidal response to thyrotropin in diabetic mice.

    abstract::The effect of diabetes mellitus on the synthesis and secretion of thyroid hormone ws investigated in mice with streptozotocin-induced diabetes. Thyroid glands were labeled in vivo with 131I for 2 h. In control animals, TSH stimulated the synthesis of PB127I and 131I-labeled iodothyronines and simultaneously decreased ...


    pub_type: 杂志文章


    authors: Bagchi N,Brown TR,Shivers B,Lucas S,Mack RE

    更新日期:1981-11-01 00:00:00

  • Graves' hyperthyroidism and the hygiene hypothesis in a mouse model.

    abstract::Graves' hyperthyroidism is an organ-specific autoimmune disease mediated by stimulatory autoantibodies against the TSH receptor (TSHR; thyroid-stimulating antibodies), causing thyroid hyperplasia and hyperthyroidism. Development of this ailment is well known to be under polygenic and environmental control. For example...


    pub_type: 杂志文章


    authors: Nagayama Y,McLachlan SM,Rapoport B,Oishi K

    更新日期:2004-11-01 00:00:00

  • Starting the zebrafish pineal circadian clock with a single photic transition.

    abstract::The issue of what starts the circadian clock ticking was addressed by studying the developmental appearance of the daily rhythm in the expression of two genes in the zebrafish pineal gland that are part of the circadian clock system. One encodes the photopigment exorhodopsin and the other the melatonin synthesizing en...


    pub_type: 杂志文章


    authors: Vuilleumier R,Besseau L,Boeuf G,Piparelli A,Gothilf Y,Gehring WG,Klein DC,Falcón J

    更新日期:2006-05-01 00:00:00

  • Alpha, beta, and gamma mineralocorticoid receptor messenger ribonucleic acid splice variants: differential expression and rapid regulation in the developing hippocampus.

    abstract::Two different types of corticoid receptor molecules bind circulating corticosterone in brain: mineralocorticoid receptors (MR) and glucocorticoid receptors. MR exhibit the highest affinity for the endogenous glucocorticoid in the rat, corticosterone. During development, low corticosterone levels influence neurogenesis...


    pub_type: 杂志文章


    authors: Vázquez DM,López JF,Morano MI,Kwak SP,Watson SJ,Akil H

    更新日期:1998-07-01 00:00:00

  • Aprotinin antagonizes the binding of human chorionic gonadotropin to its receptor.

    abstract::Aprotinin caused a dose-dependent inhibition of [125I]hCG binding to its receptor in plasma membranes prepared from luteinized rat ovaries. Displacement of [125I]hCG from its receptor by aprotinin was temperature dependent, with an ID50 of 300 microM at 4 C and an ID50 of 3700 microM at 22 C. Equilibrium binding data ...


    pub_type: 杂志文章


    authors: Wilks JW,Hui OJ

    更新日期:1987-03-01 00:00:00

  • Inactivation of early glucocorticoid feedback by corticotropin-releasing factor in vitro.

    abstract::We have investigated the interaction between hypothalamic ACTH secretagogues and adrenocortical glucocorticoids in rat anterior pituitary tissue using an in vitro perifusion system. Repeated 5 min pulses of 41-residue CRF (CRF-41) or arginine vasopressin (AVP) were applied at 1 h intervals for up to 7 h. Administratio...


    pub_type: 杂志文章


    authors: Shipston MJ,Antoni FA

    更新日期:1992-04-01 00:00:00

  • An intramolecular disulfide bond between conserved extracellular cysteines in the gonadotropin-releasing hormone receptor is essential for binding and activation.

    abstract::In this study, site-directed mutagenesis and biochemical strategies have been used to establish whether disulfide bonding between extracellular Cys residues contributes to the structural integrity of the GnRH receptor (GnRH-R) and, if so, to delineate the nature of the bonding patterns involved. The majority of G prot...


    pub_type: 杂志文章


    authors: Cook JV,Eidne KA

    更新日期:1997-07-01 00:00:00

  • Down regulation of insulin receptors in primary cultures of adult rat hepatocytes in monolayer.

    abstract::Down regulation of insulin receptor number by insulin at physiological concentrating (10(-9) M) has been demonstrated in primary cultures of adult rat liver parenchymal cells in monolayer. However, because insulin is rapidly degraded in this culture system, special efforts are necessary to maintain the concentration o...


    pub_type: 杂志文章


    authors: Blackard WG,Guzelian PS,Small ME

    更新日期:1978-08-01 00:00:00

  • Regulation of the hypothalamic-pituitary-adrenal axis circadian rhythm by endocannabinoids is sexually diergic.

    abstract::We have examined the effects of acute administration of the cannabinoid receptor type 1 (CB(1)) antagonist AM251 on the rat hypothalamic-pituitary-adrenal (HPA) axis with respect to both gender and time of day. Blood samples were collected from conscious male and female rats every 5 min using an automated blood sampli...


    pub_type: 杂志文章


    authors: Atkinson HC,Leggett JD,Wood SA,Castrique ES,Kershaw YM,Lightman SL

    更新日期:2010-08-01 00:00:00

  • Estrogen receptor (ER)alpha and ERbeta exhibit unique pharmacologic properties when coupled to activation of the mitogen-activated protein kinase pathway.

    abstract::The rapid, nongenomic effects of estrogen are increasingly recognized as playing an important role in several aspects of estrogen action. Rapid activation of the mitogen-activated protein kinase (MAPK) signaling pathway by estrogen is among the more recently identified of these effects. To explore the role of estrogen...


    pub_type: 杂志文章


    authors: Wade CB,Robinson S,Shapiro RA,Dorsa DM

    更新日期:2001-06-01 00:00:00

  • Altered endocrine and autocrine metabolism of vitamin D in a mouse model of gastrointestinal inflammation.

    abstract::The active form of vitamin D, 1,25-dihydroxyvitamin D3, [1,25(OH)2D3] has potent actions on innate and adaptive immunity. Although endocrine synthesis of 1,25(OH)2D3 takes place in the kidney, the enzyme that catalyzes this, 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27b1 in humans, Cyp27b1 in mice), is expressed at m...


    pub_type: 杂志文章


    authors: Liu N,Nguyen L,Chun RF,Lagishetty V,Ren S,Wu S,Hollis B,DeLuca HF,Adams JS,Hewison M

    更新日期:2008-10-01 00:00:00

  • Reduced ghrelin, islet amyloid polypeptide, and peptide YY expression in the stomach of gastrin-cholecystokinin knockout mice.

    abstract::The antral hormone gastrin and its intestinal relative, cholecystokinin (CCK), are pivotal in the regulation of gastric functions. Other gastric hormones like ghrelin, peptide YY (PYY), and islet amyloid polypeptide (IAPP), however, also contribute to the regulation of acid secretion, motility, and feeding. Because ga...


    pub_type: 杂志文章


    authors: Friis-Hansen L,Wierup N,Rehfeld JF,Sundler F

    更新日期:2005-10-01 00:00:00

  • A monoclonal antibody against bovine anti-Müllerian hormone.

    abstract::Anti-Müllerian hormone (AMH) was partially purified from incubation medium of calf fetal testes and injected into a BALB/c mouse, whose splenocytes were fused with Sp2/Ag8 myeloma cells. Hybridomas were screened for specific antibody production by double antibody precipitation of labeled AMH, which was obtained by inc...


    pub_type: 杂志文章


    authors: Vigier B,Picard JY,Josso N

    更新日期:1982-01-01 00:00:00

  • Role and regulation of adipokines during zymosan-induced peritoneal inflammation in mice.

    abstract::Adipokines, cytokines mainly produced by adipocytes, are active participants in the regulation of inflammation. Administration of zymosan (ZY) was used to investigate the regulation and role of adipokines during peritonitis in mice. Injection of ZY led to a significant increase in leptin levels in both serum and perit...


    pub_type: 杂志文章


    authors: Pini M,Gove ME,Sennello JA,van Baal JW,Chan L,Fantuzzi G

    更新日期:2008-08-01 00:00:00