Abstract:
:Previously, we demonstrated that excision of the GnRH first intron (intron A) was largely attenuated in non-GnRH-producing tissues but accelerated in GnRH neurons. In the present study, we examined the splicing rate of GnRH pre-mRNA in developing normal mice and adult hypogonadal mice. The preoptic area and cerebral cortex were removed from mice at ages 1-7 wk. GnRH pre-mRNA splicing was examined by competitive RT-PCR using a variety of primer sets. The ratio of mature mRNA to intron A-containing RNA species in the preoptic area was lowest in 1- and 2-wk-old mice, significantly augmented in 3-wk-old mice, and further increased until adulthood. In contrast, the ratio of mRNA to intron A-containing RNA in the cerebral cortex was extremely low, drastically decreased in 3-wk-old mice, and remained at low levels until adulthood. These data indicate a preoptic area-specific increase in intron A excision during development. Intron B or C excision in the preoptic area was not significantly changed during development. To elucidate the possible involvement of the exonic splicing enhancers located in GnRH exons 3 and 4 in the developmental increase in intron A excision, we examined the splicing rate of GnRH pre-mRNA in hypogonadal mice whose GnRH exons 3 and 4 were truncated. The intron A excision in the preoptic area of hypogonadal mice was significantly lower than that of normal mice but similar to that in other tissues, such as cerebral cortex and olfactory bulb. To support the functional relevance of intron A-containing RNA species, we examined the translation efficiency of intron A-containing RNA. Insertion of intron A sequence into the upstream portion of the luciferase open reading frame significantly decreased translation efficiency. The present study demonstrates that intron A excision in the preoptic area is developmentally regulated in normal mice but largely attenuated in hypogonadal mice, indicating the functional importance of intron A excision in GnRH pre-mRNA splicing.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Seong JY,Kim BW,Park S,Son GH,Kim Kdoi
10.1210/endo.142.10.8449subject
Has Abstractpub_date
2001-10-01 00:00:00pages
4454-61issue
10eissn
0013-7227issn
1945-7170journal_volume
142pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0473
更新日期:2006-09-01 00:00:00
abstract::The procession of round spermatids through stages VII and VIII of the rat spermatogenic cycle is critically dependent on testosterone (T). When intratesticular T levels are reduced, round spermatids appear to slough from the seminiferous epithelium, resulting in the disappearance of elongated spermatids. We hypothesiz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.9.8756560
更新日期:1996-09-01 00:00:00
abstract::Transforming growth factor-beta (TGFbeta) and adrenomedullin (AM) are multifunctional regulatory peptides that are secreted by a variety of normal and malignant cells. The TGFbetas are expressed in developing organs and adults, and their tissue distribution pattern has possible significance for signaling roles in many...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.9.6205
更新日期:1998-09-01 00:00:00
abstract::Compelling evidence indicates a crucial role of prostaglandin F2α (PGF2α) in parturition. Both the maternal and fetal sides of the fetal membranes synthesize PGF2α, which exerts effects via the prostaglandin F2α receptor (FP) that is coupled to the activation of protein kinase C (PKC). Cyclooxygenase-2 (COX-2) catalyz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1441
更新日期:2012-10-01 00:00:00
abstract::The maternal skeleton resorbs during lactation to provide calcium to milk and the lost mineral content is restored after weaning. The changes are particularly marked in Ctcgrp null mice, which lose 50% of spine mineral content during lactation but restore it fully. The known calciotropic hormones are not required for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1931
更新日期:2013-04-01 00:00:00
abstract::We describe a new animal model of obesity and GH deficiency and report the effects on body fat of administering (GH) and insulin-like growth factor (IGF-I) in the model. Female GH-deficient dwarf rats fed a high-fat diet became obese and insulin-resistant compared with chow-fed controls. They were treated with recombi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612530
更新日期:1996-05-01 00:00:00
abstract::A midregion fragment of PTH-related protein (PTHrP), which is intensively conserved across species, has been identified as a secretory product of several different cell types, including keratinocytes and squamous carcinomas. As recent data suggest that a midregion PTHrP fragment may be biologically active, we hypothes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940360
更新日期:1996-12-01 00:00:00
abstract::Estrogen inhibits postmenopausal bone loss and decreases fracture risk. Unfortunately, estrogen replacement therapy has many undesirable side effects, the majority of which are due to stimulation of reproductive tissues. Tissue specific estrogen agonists provide a promising new alternative to natural estrogens for hor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5109
更新日期:1997-05-01 00:00:00
abstract::The purpose of these experiments was to determine if the powerful effect of sodium chloride concentration on angiotensin II- and potassium-stimulated aldosterone secretion by isolated perfused adrenal glands is mediated by the sodium or chloride ion or by the obligatory change in osmolality. We used isolated Ringer's ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1621
更新日期:1985-04-01 00:00:00
abstract::Neuropeptide Y (NPY) peptide concentrations in the arcuate nucleus have recently been shown to be modulated by gonadal steroids in the male rat. The present study was designed to determine whether NPY messenger RNA (mRNA)-synthesizing cells in the arcuate nucleus (Arc) of the male rat are regulated by testosterone (T)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419120
更新日期:1993-01-01 00:00:00
abstract::Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells. Death factors such as Fas ligand induce apoptosis by triggering a series of intracellular protein-protein interactions mediated by defined motifs found in the signaling molecules. One of these motifs is the death effector d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6335
更新日期:1998-12-01 00:00:00
abstract::GnRH autoregulates GnRH neurons through an ultrashort feedback loop. One potential mechanism is the regulation of K(+) channel activity through the GnRH receptor. Whereas GnRH inhibits the activity of the M-current in peripheral neurons, there is no direct evidence that the M-current is involved in the autoregulatory ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1178
更新日期:2008-05-01 00:00:00
abstract::Even though functional CRH receptors have been identified in several brain regions by ligand binding, the identity of brain areas expressing the CRH receptor gene has not been described. The recent cloning of the rat CRH receptor gene has permitted us to conduct an in situ hybridization histochemistry study to localiz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7956950
更新日期:1994-11-01 00:00:00
abstract::T3 is required for normal skeletal development, but its cellular targets in bone are unknown. T3 regulates target gene transcription via a specific nuclear receptor (T3R), which can heterodimerize with 9-cis-retinoic acid, 1 alpha, 25-dihydroxyvitamin D3, or retinoic acid receptors to modify T3 responsiveness. Serum-f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988420
更新日期:1994-12-01 00:00:00
abstract::The regression of the tadpole tail is under the direct control of the thyroid hormones and offers a unique system for the study of the action of these hormones. We have examined the binding of L-triiodothyronine (T3) and L-thyroxine (T4) in vitro using tail fin bricks which included epidermal and connective cells. The...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-1030
更新日期:1975-10-01 00:00:00
abstract::We have examined the regulation of an insulin-like growth factor-binding protein-3 (IGFBP-3) protease secreted by MCF-7 human breast cancer cells using a ligand-binding assay that relies on the decrease in affinity for des(1-3)IGF-I that occurs when IGFBP-3 becomes proteolyzed. IGFBP-3 protease activity was not altere...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.9.7684
更新日期:2000-09-01 00:00:00
abstract::Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2421
更新日期:1987-06-01 00:00:00
abstract::We analyzed the mechanism by which a Graves' disease-associated C/T polymorphism in the Kozak sequence of CD40 affects CD40 expression. CD40 expression levels on B cells in individuals with CT and TT genotypes were decreased by 13.3 and 39.4%, respectively, compared with the levels in CC genotypes (P = 0.012). Similar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1617
更新日期:2005-06-01 00:00:00
abstract::The ovulatory LH surge results in follicular inflammation with an increase in cytokines and PGs. Reactive oxygen species (ROS) are also produced during inflammatory processes. To study ROS generation during the ovulatory cascade, preovulatory follicles were dissected from immature female rats primed with PMSG. Follicl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.7961
更新日期:2001-02-01 00:00:00
abstract::The influence of LY117018 and tamoxifen on established uterotropic influence of estradiol was examined in immature ovariectomized rats. When LY117018 was introduced on the fourth day of estradiol treatment, it regressed the uterotropic effect of estradiol. However, tamoxifen did not attenuate estradiol activity under ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-3-987
更新日期:1981-09-01 00:00:00
abstract::The lipid phosphatase, phosphatase and tensin homolog (PTEN), is a key element in controlling cell growth and survival and has a well established role as tumor suppressor protein in many neoplasia. Several data indicate that silencing of PTEN gene expression may be relevant in follicular thyroid cell transformation. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0282
更新日期:2004-10-01 00:00:00
abstract::Protein kinase-C (PKC) has been suggested as a possible mediator of the antigonadotropic action of prostaglandin F2 alpha (PGF2 alpha) in luteal cells. To examine this possibility, we evaluated the effects of phorbol ester [12-O-tetradecanoylphorbol-13-acetate (TPA)] in relation to those of PGF2 alpha on cAMP accumula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1388
更新日期:1990-03-01 00:00:00
abstract::Angiotensin II and extracellular potassium stimulate aldosterone production in adrenal glomerulosa cells by mobilizing the calcium messenger system. This response requires calcium influx across the plasma membrane, followed by calcium uptake into the mitochondria. It has been proposed that calcium is transported to th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0268
更新日期:2003-10-01 00:00:00
abstract::Lanosterol 14alpha-demethylase (CYP51) responds to cholesterol feedback regulation through sterol regulatory element binding proteins (SREBPs). The proximal promoter of CYP51 contains a conserved region with clustered regulatory elements: GC box, cAMP-response elements (CRE-like), and sterol regulatory element (SRE). ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0781
更新日期:2005-12-01 00:00:00
abstract::Prolonged exposure to elevated glucocorticoid levels is known to produce insulin resistance (IR), a hallmark of diabetes mellitus. Although not fully elucidated, the underlying molecular mechanisms by which glucocorticoids induce IR may provide potential targets for pharmacological interventions. Here we characterized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1214
更新日期:2008-02-01 00:00:00
abstract::Pheochromocytomas (PCCs) are tumors arising from neural crest-derived chromaffin cells. There are currently few animal models of PCC that recapitulate the key features of human tumors. Because such models may be useful for investigations of molecular pathomechanisms and development of novel therapeutic interventions, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1108
更新日期:2016-08-01 00:00:00
abstract::The BB/Wor rat develops spontaneous autoimmune diabetes mellitus and also frequently develops lymphocytic thyroiditis. To clarify the role of T cell lymphopenia and the major histocompatibility complex (MHC) in the development of these autoimmune disorders, we studied back-cross animals between the inbred thyroiditis ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588330
更新日期:1995-12-01 00:00:00
abstract::Dispersed cells from whole testes or from isolated interstitial tissue of mature rats, yielded two distinct populations of Leydig cells when subjected to centrifugation in a 0-40% metrizamide gradient. One population (I) was found in a fractions with a density of 1.085-1.117 g/cm3, and the other population (II) was fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-5-1424
更新日期:1980-05-01 00:00:00
abstract::Whole saliva collected from rat, mouse, and human sources was found to contain high concentrations of transforming growth factor-alpha (TGF alpha) when analyzed by RIA. The concentrations of TGF alpha in unstimulated human saliva (age, 30-45 yr; n = 10; 1.5 +/- 3.1 nM) was reduced with age (age, 55-70 yr; n = 10; 0.4 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299590
更新日期:1994-02-01 00:00:00
abstract::Embryo implantation is a highly synchronized event between an activated blastocyst and a receptive endometrium. The success of this process relies on the dynamic interplay of estrogen (E(2)) and progesterone (P(4)), however, the details of this interaction are not entirely clear. Recent data implicate E(2) and P(4) in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1081
更新日期:2009-03-01 00:00:00