Facilitative glucose transporter type 1 is differentially regulated by progesterone and estrogen in murine and human endometrial stromal cells.


:Embryo implantation is a highly synchronized event between an activated blastocyst and a receptive endometrium. The success of this process relies on the dynamic interplay of estrogen (E(2)) and progesterone (P(4)), however, the details of this interaction are not entirely clear. Recent data implicate E(2) and P(4) in the regulation of glucose utilization by affecting facilitative glucose transporter (GLUT) expression. In this study we examine GLUT1 expression in murine and human endometrial stromal cells (ESCs) using a primary culture system. We show that expression of GLUT1 is increased during ESC decidualization in vitro. P(4) up-regulates, whereas E(2) down-regulates, GLUT1 expression. In addition, P(4) increases and E(2) decreases glucose uptake in ESCs, suggesting that GLUT1 may be a major player in glucose utilization in these cells. Moreover, GLUT1 expression is increased in human ESCs when decidualized in vitro with P(4) and dibutyryl cAMP, suggesting a similar role for P(4) in human endometrium. In conclusion, an imbalance between P(4) and E(2) seen in patients with polycystic ovary syndrome, luteal phase defect, and recurrent pregnancy loss may have a critical impact on glucose utilization in the endometrial stroma, and, thus, may be responsible for endometrial dysfunction and failure of embryo implantation in these patient populations.






Frolova A,Flessner L,Chi M,Kim ST,Foyouzi-Yousefi N,Moley KH




Has Abstract


2009-03-01 00:00:00














  • Stanozolol and danazol, unlike natural androgens, interact with the low affinity glucocorticoid-binding sites from male rat liver microsomes.

    abstract::Some 17 alpha-alkylated androgens used as anabolic agents, such as stanozolol (ST) and danazol (DA), have specific effects on the liver that are not exerted by testosterone. This gives rise to the possibility that a steroid-binding protein, other than the androgen receptor, could modulate the intracellular actions of ...


    pub_type: 杂志文章


    authors: Fernández L,Chirino R,Boada LD,Navarro D,Cabrera N,del Rio I,Díaz-Chico BN

    更新日期:1994-03-01 00:00:00

  • Circadian and Ultradian Variations in Corticosterone Level Influence Functioning of the Male Mouse Basolateral Amygdala.

    abstract::The hypothalamic-pituitary-adrenal axis involves timed signaling between the hypothalamus, pituitary, and adrenal glands and back to the brain, causing an inherently oscillating system. Corticosteroids such as corticosterone (CORT) are secreted in a circadian rhythm, characterized by low and high levels at the start o...


    pub_type: 杂志文章


    authors: den Boon FS,de Vries T,Baelde M,Joëls M,Karst H

    更新日期:2019-04-01 00:00:00

  • Hypothalamic interleukin-1 beta and tumor necrosis factor-alpha, but not interleukin-6, mediate the endotoxin-induced suppression of the reproductive axis in rats.

    abstract::It is well established that endotoxemia disrupts reproductive capability, and several proinflammatory cytokines, especially IL-1 beta, IL-6, and TNF-alpha in the brain, have been implicated in this endocrine aberration. However, no previous study has directly compared the effects of the three major proinflammatory cyt...


    pub_type: 杂志文章


    authors: Watanobe H,Hayakawa Y

    更新日期:2003-11-01 00:00:00

  • Dissociation of actin polymerization and enzyme inactivation in the hormonal regulation of type II iodothyronine 5'-deiodinase activity in astrocytes.

    abstract::T4 dynamically regulates the levels of type II iodothyronine 5'-deiodinase in the brain. Using an astrocyte cell culture model, we have shown that thyroxine increases inactivation of this enzyme through a mechanism using the actin cytoskeleton. In the absence of T4, the filamentous actin (F-actin) stress fibers are ab...


    pub_type: 杂志文章


    authors: Farwell AP,Leonard JL

    更新日期:1992-08-01 00:00:00

  • Pituitary corticotrope tumor (AtT20) cells as a model system for the study of early inhibition by glucocorticoids.

    abstract::The utility of the established ACTH secreting mouse pituitary tumor cell line AtT20 for investigating early glucocorticoid inhibition was examined. Three different strains of the cell line D1, D16v, and D16:16, respectively, were analyzed. In initial studies CRF and phorbol esters were used as secretagogues to examine...


    pub_type: 杂志文章


    authors: Woods MD,Shipston MJ,Mullens EL,Antoni FA

    更新日期:1992-12-01 00:00:00

  • Characterization of an estrogen-binding protein in the yeast Candida albicans.

    abstract::An estrogen-binding protein (EBP) has been identified and characterized in the cytosol of the pathogenic yeast Candida albicans. Binding of [3H]estradiol was found to be optimal at pH 7.4 in the presence of 0.3 M KCl and was linearly related to protein concentration. Binding was very rapid, reaching maximal levels in ...


    pub_type: 杂志文章


    authors: Skowronski R,Feldman D

    更新日期:1989-04-01 00:00:00

  • Elevated levels of epidermal growth factor receptor/c-erbB2 heterodimers mediate an autocrine growth regulatory pathway in tamoxifen-resistant MCF-7 cells.

    abstract::The development of acquired resistance to antihormonal agents in breast cancer is a major therapeutic problem. We have developed a tamoxifen-resistant (TAM-R) MCF-7 breast cancer cell line to investigate the mechanisms behind this condition. Both epidermal growth factor receptor (EGFR) and c-erbB2 mRNA and protein exp...


    pub_type: 杂志文章


    authors: Knowlden JM,Hutcheson IR,Jones HE,Madden T,Gee JM,Harper ME,Barrow D,Wakeling AE,Nicholson RI

    更新日期:2003-03-01 00:00:00

  • Activation of cell adhesion kinase beta by mechanical stretch in vascular smooth muscle cells.

    abstract::We have studied whether activation of cell adhesion kinase beta (CAKbeta) is involved in stretch-induced signaling pathway in cultured rat vascular smooth muscle cells. Cyclic stretch (1 Hz) induced a rapid (within 1 min) phosphorylation of CAKbeta, whose effect was time and strength dependent. Both Ca(2+) and Na(+) i...


    pub_type: 杂志文章


    authors: Iwasaki H,Yoshimoto T,Sugiyama T,Hirata Y

    更新日期:2003-06-01 00:00:00

  • Local effects of cholesterol carrier system on binding of lactogens and epidermal growth factor to the developing mammary gland.

    abstract::Cholesterol pellets inserted directly into mammary glands of 4- and 10-week-old mice resulted in lactogens, such as human growth hormone and prolactin, and epidermal growth factor. The effects of cholesterol the binding of lactogens and epidermal growth factor to the livers of these same animals was unaffected. Pellet...


    pub_type: 杂志文章


    authors: Vonderhaar BK

    更新日期:1993-07-01 00:00:00

  • Evidence for a possible role for Ca++ in the 3,5,3'-triiodothyronine inhibition of thyrotropin-releasing hormone-induced secretion of thyrotropin by rat anterior pituitary in vitro.

    abstract::The present study was undertaken to determine whether T3 could modify anterior pituitary Ca++ metabolism under basal conditions and in the presence of TRH. Paired hemipituitaries from hypothyroid rats were preincubated with T3 (10(-7) M) for 2 h, allowed to accumulate 45Ca++ for the third hour, and repeatedly washed i...


    pub_type: 杂志文章


    authors: Schrey MP,Larsen PR

    更新日期:1981-05-01 00:00:00

  • A putative binding site for Sp1 is involved in transcriptional regulation of CYP17 gene expression in bovine ovary.

    abstract::In the bovine ovary, thecal cells are the only cell type capable of expressing the CYP17 gene in response to LH. With the onset of ovulation and luteinization in the cow, there is complete loss of P450c17alpha expression. To characterize the molecular mechanisms involved in tissue-specific regulation of the CYP17 gene...


    pub_type: 杂志文章


    authors: Borroni R,Liu Z,Simpson ER,Hinshelwood MM

    更新日期:1997-05-01 00:00:00

  • Expression of calbindin-D decreases with age in intestine and kidney.

    abstract::The calbindins are Ca-binding proteins whose expression is regulated by 1,25-dihydroxyvitamin D3, the active metabolite of vitamin D3. The calbindins are found in high amounts in the proximal intestine (calbindin-D-9k) and the kidney (calbindin-D-28k), and they are thought to play a role in Ca transport by these tissu...


    pub_type: 杂志文章


    authors: Armbrecht HJ,Boltz M,Strong R,Richardson A,Bruns ME,Christakos S

    更新日期:1989-12-01 00:00:00

  • Reversal of defective glucagon responses to hypoglycemia in insulin-dependent autoimmune diabetic BB rats.

    abstract::The intraislet insulin hypothesis has been proposed to explain absent glucagon responses to hypoglycemia. Recently we directly confirmed this hypothesis by restoring glucagon secretion via provision of a pancreatic artery insulin infusion, which was switched off at the time of hypoglycemia in Wistar rats made diabetic...


    pub_type: 杂志文章


    authors: Zhou H,Zhang T,Oseid E,Harmon J,Tonooka N,Robertson RP

    更新日期:2007-06-01 00:00:00

  • Transactivation of the parathyroid hormone promoter by specificity proteins and the nuclear factor Y complex.

    abstract::We previously identified a highly conserved specificity protein 1 (Sp1) DNA element in mammalian PTH promoters that acted as an enhancer of gene transcription and bound Sp1 and Sp3 proteins present in parathyroid gland nuclear extracts. More recently, a nuclear factor (NF)-Y element (NF-Y(prox)) was also described by ...


    pub_type: 杂志文章


    authors: Alimov AP,Park-Sarge OK,Sarge KD,Malluche HH,Koszewski NJ

    更新日期:2005-08-01 00:00:00

  • Thyroid hormone enhances aggrecanase-2/ADAM-TS5 expression and proteoglycan degradation in growth plate cartilage.

    abstract::Effects of thyroid hormone on proteoglycan degradation in various regions of cartilage were investigated. In propylthiouracil-treated rats with hypothyroidism, proteoglycan degradation in epiphyseal cartilage during endochondral ossification was markedly suppressed. However, injections of T(4) reversed this effect of ...


    pub_type: 杂志文章


    authors: Makihira S,Yan W,Murakami H,Furukawa M,Kawai T,Nikawa H,Yoshida E,Hamada T,Okada Y,Kato Y

    更新日期:2003-06-01 00:00:00

  • Developmental study of androgen responsiveness in the submandibular gland of the mouse.

    abstract::The development of androgen responsiveness in the submandibular gland of normal and androgen-resistant (Tfm) mice of different ages was studied after varying hormonal treatments. Total esteroproteolytic (tamase) activity of submandibular gland homogenates was used as a marker for androgen action. Newborn mice of all f...


    pub_type: 杂志文章


    authors: Dunn JF,Wilson JD

    更新日期:1975-06-01 00:00:00

  • Angiotensin II increases lipogenesis in 3T3-L1 and human adipose cells.

    abstract::Angiotensin II (Ang II) is one of numerous hormones recently shown to be synthesized and secreted by adipose cells. Although the function of Ang II in adipose tissue is unknown, several studies indirectly suggest that it may be involved in control of adiposity. Little is known, however, about direct actions of Ang II ...


    pub_type: 杂志文章


    authors: Jones BH,Standridge MK,Moustaid N

    更新日期:1997-04-01 00:00:00

  • Thyrotropin-induced acceleration of calcium efflux from mouse thyroid: evidence for inhibition by excess iodide.

    abstract::We investigated the effect of TSH on calcium transport in mouse thyroid, as well as the influence of iodide thereupon. Thyroid lobes were incubated in Krebs-Ringer bicarbonate buffer containing [45Ca2+] and the time-dependent uptake of [45Ca2+] by the lobes was observed. In the presence of 0.5 mU/ml TSH, the uptake of...


    pub_type: 杂志文章


    authors: Hashizume K,Ichikawa K,Komiya I,Onaya T

    更新日期:1984-05-01 00:00:00

  • Caudal brainstem processing is sufficient for behavioral, sympathetic, and parasympathetic responses driven by peripheral and hindbrain glucagon-like-peptide-1 receptor stimulation.

    abstract::The effects of peripheral glucagon like peptide-1 receptor (GLP-1R) stimulation on feeding, gastric emptying, and energetic responses involve vagal transmission and central nervous system processing. Despite a lack of studies aimed at determining which central nervous system regions are critical for the GLP-1R respons...


    pub_type: 杂志文章


    authors: Hayes MR,Skibicka KP,Grill HJ

    更新日期:2008-08-01 00:00:00

  • N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.

    abstract::For successful human placentation, invasion of trophoblast cells into the uterus and its associated vasculature is essential, and the regulation of this process is controlled by many factors at the fetal-maternal interface. N-acetylglucosaminyltransferase V (GnT-V) is a key enzyme that catalyzes beta1, 6-N-acetylgluco...


    pub_type: 杂志文章


    authors: Yamamoto E,Ino K,Miyoshi E,Inamori K,Abe A,Sumigama S,Iwase A,Kajiyama H,Shibata K,Nawa A,Kikkawa F

    更新日期:2009-02-01 00:00:00

  • Effects of somatostatin and urotensin II on tilapia pituitary prolactin release and interactions between somatostatin, osmotic pressure Ca++, and adenosine 3',5'-monophosphate in prolactin release in vitro.

    abstract::Both somatostatin (SRIF) and urotensin II, a dodecapeptide from the teleost caudal neurosecretory system, inhibit PRL release from the organ-cultured rostral pars distalis of the tilapia, Sarotherodon mossambicus, in a dose-related manner. The inhibitory action of SRIF on PRL release was completely prevented by the pr...


    pub_type: 杂志文章


    authors: Grau EG,Nishioka RS,Bern HA

    更新日期:1982-03-01 00:00:00

  • Liver X receptor agonist T0901317 inhibition of glucocorticoid receptor expression in hepatocytes may contribute to the amelioration of diabetic syndrome in db/db mice.

    abstract::The glucocorticoid receptor (GR) is a crucial target gene for glucocorticoid-induced insulin resistance and hepatic gluconeogenesis linked to the development of type 2 diabetes. The liver X receptors (LXRs) are nuclear receptors that play an important role in the regulation of the metabolic gene linked to carbohydrate...


    pub_type: 杂志文章


    authors: Liu Y,Yan C,Wang Y,Nakagawa Y,Nerio N,Anghel A,Lutfy K,Friedman TC

    更新日期:2006-11-01 00:00:00

  • The type 2 iodothyronine deiodinase is expressed in the rat uterus and induced during pregnancy.

    abstract::Thyroid hormones are of considerable importance for vertebrate reproductive function and during development. To further assess the role of these compounds in this capacity, we examined the expression pattern of the type 2 iodothyronine deiodinase (D2), which converts T(4) to the more active hormone T(3), in the rat ut...


    pub_type: 杂志文章


    authors: Galton VA,Martinez E,Hernandez A,St Germain EA,Bates JM,St Germain DL

    更新日期:2001-05-01 00:00:00

  • Effect of bisphosphonates on the rapidly growing male murine skeleton.

    abstract::Bisphosphonates are effective for preventing and treating skeletal disorders associated with hyperresorption. Their safety and efficacy has been studied in adults where the growth plate is fused and there is no longitudinal bone growth and little appositional growth. Although bisphosphonate use in the pediatric popula...


    pub_type: 杂志文章


    authors: Zhu ED,Louis L,Brooks DJ,Bouxsein ML,Demay MB

    更新日期:2014-04-01 00:00:00

  • Positive and negative regulation of the rat vasopressin gene promoter.

    abstract::To study the transcriptional regulation of the vasopressin gene in vitro, 3 kb of the 5' regulatory region of the rat vasopressin gene was isolated and subcloned, along with a series of various deletion mutants, into vectors containing the luciferase reporter gene. After transfecting these genes transiently into the h...


    pub_type: 杂志文章


    authors: Iwasaki Y,Oiso Y,Saito H,Majzoub JA

    更新日期:1997-12-01 00:00:00

  • Diurnal variation in resting levels of corticosterone is not mediated by variation in adrenal responsiveness to adrenocorticotropin but involves splanchnic nerve integrity.

    abstract::To study possible mechanisms controlling diurnal changes in corticosterone (CORT) levels, we tested the CORT responses to ACTH in the morning (AM) and evening (PM) in male Wistar and Sprague-Dawley rats. Rat ACTH-(1-39) or human ACTH-(1-24) (3.75-15 ng/rat) was given as an iv bolus or an intraarterial infusion to (un)...


    pub_type: 杂志文章


    authors: Dijkstra I,Binnekade R,Tilders FJ

    更新日期:1996-02-01 00:00:00

  • GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes.

    abstract::The expression of short-chain fatty acid receptors GPR41/FFAR3 and GPR43/ free fatty acid receptor 2 (FFAR2) was studied in the gastrointestinal tract of transgenic monomeric red fluorescent protein (mRFP) reporter mice. In the stomach free fatty acid receptor 3 (FFAR3)-mRFP was expressed in a subpopulation of ghrelin...


    pub_type: 杂志文章


    authors: Nøhr MK,Pedersen MH,Gille A,Egerod KL,Engelstoft MS,Husted AS,Sichlau RM,Grunddal KV,Poulsen SS,Han S,Jones RM,Offermanns S,Schwartz TW

    更新日期:2013-10-01 00:00:00

  • Possible mechanism of action of metoclopramide-induced aldosterone secretion: in vivo and in vitro studies in the sheep.

    abstract::To define further the mechanism by which metoclopramide, a dopamine antagonist, stimulates aldosterone secretion, seven lambs were injected iv with metoclopramide during a constant infusion of 5% dextrose in water and again during a constant infusion of trimethapan, a ganglionic blocker. In addition, suspensions of ad...


    pub_type: 杂志文章


    authors: Wilson TA,Kaiser DL,Peach MJ,Wright EM,Carey RM

    更新日期:1983-09-01 00:00:00

  • Detection of a kallikrein in the mouse lactating mammary gland: a possible processing enzyme for the epidermal growth factor precursor.

    abstract::Kallikreins are a multigene subfamily of serine proteases that may have a role in processing precursors of polypeptide hormones and growth factors. The epidermal growth factor (EGF) immunoreactivity in mouse milk is derived from the membrane-bound EGF precursor located on the lumenal border of the alveolar cells in th...


    pub_type: 杂志文章


    authors: Jahnke GD,Chao J,Walker MP,Diaugustine RP

    更新日期:1994-11-01 00:00:00

  • Glucocorticoids inhibit keratinocyte growth factor production in primary dermal fibroblasts.

    abstract::The participation of growth factors in wound healing and tissue repair has been well established. Previous studies demonstrated that the expression of keratinocyte growth factor (KGF) was greatly elevated shortly after injury and that topical application of KGF accelerated healing. Steroidal antiinflammatory agents, s...


    pub_type: 杂志文章


    authors: Chedid M,Hoyle JR,Csaky KG,Rubin JS

    更新日期:1996-06-01 00:00:00