Abstract:
:Embryo implantation is a highly synchronized event between an activated blastocyst and a receptive endometrium. The success of this process relies on the dynamic interplay of estrogen (E(2)) and progesterone (P(4)), however, the details of this interaction are not entirely clear. Recent data implicate E(2) and P(4) in the regulation of glucose utilization by affecting facilitative glucose transporter (GLUT) expression. In this study we examine GLUT1 expression in murine and human endometrial stromal cells (ESCs) using a primary culture system. We show that expression of GLUT1 is increased during ESC decidualization in vitro. P(4) up-regulates, whereas E(2) down-regulates, GLUT1 expression. In addition, P(4) increases and E(2) decreases glucose uptake in ESCs, suggesting that GLUT1 may be a major player in glucose utilization in these cells. Moreover, GLUT1 expression is increased in human ESCs when decidualized in vitro with P(4) and dibutyryl cAMP, suggesting a similar role for P(4) in human endometrium. In conclusion, an imbalance between P(4) and E(2) seen in patients with polycystic ovary syndrome, luteal phase defect, and recurrent pregnancy loss may have a critical impact on glucose utilization in the endometrial stroma, and, thus, may be responsible for endometrial dysfunction and failure of embryo implantation in these patient populations.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Frolova A,Flessner L,Chi M,Kim ST,Foyouzi-Yousefi N,Moley KHdoi
10.1210/en.2008-1081subject
Has Abstractpub_date
2009-03-01 00:00:00pages
1512-20issue
3eissn
0013-7227issn
1945-7170pii
en.2008-1081journal_volume
150pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::High-fat diet and intrauterine growth retardation may predispose to obesity, insulin resistance, and type 2 diabetes. Because prenatal ethanol (ETOH) exposure causes intrauterine growth retardation, we investigated its interactions with postnatal high-fat diet on glucose tolerance and adipocyte-derived hormones in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220623
更新日期:2003-02-01 00:00:00
abstract::Naturally occurring and synthetic plant flavonoids, such as EMD 21388, are potent inhibitors of thyroid hormone 5'-deiodinase (5'-D) in vitro, but not when given in vivo, since they are tightly bound by serum transthyretin (TTR). EMD 21388 also inhibits the binding of T4 to human, dog, and rat serum TTR in vitro and w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-2890
更新日期:1990-06-01 00:00:00
abstract::We have previously shown that repeated antenatal synthetic glucocorticoid exposure has sex-specific effects on hypothalamic-pituitary-adrenal development in the fetal and adult guinea pig. However, little is known about the mechanisms that underlie these sex-specific outcomes. In the current study we demonstrated that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0145
更新日期:2003-07-01 00:00:00
abstract::Changes in several extracellular matrix proteins, such as fibronectin in fetal membrane and cervical collagens, occur at term and preterm delivery. However, no studies have evaluated changes in extracellular matrix proteins, in relation to myometrial activation recorded using invasive techniques during parturition in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6697
更新日期:1999-05-01 00:00:00
abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0473
更新日期:2006-09-01 00:00:00
abstract::Steroidogenic acute regulatory protein (StAR) synthesis and steroidogenesis are stimulated by activation of divergent signaling pathways in the adrenal cortex. The two major physiological regulators of aldosterone synthesis in the adrenal zona glomerulosa are angiotensin II (AII) and extracellular K+, which both media...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7085
更新日期:1999-10-01 00:00:00
abstract::Trans-10, cis-12 conjugated linoleic acid (10,12 CLA) is a dietary fatty acid that promotes weight loss and disproportionate fat loss. Obese mice fed a high-fat, high-sucrose (HFHS) diet containing 10,12 CLA are resistant to weight gain and contain markedly reduced subcutaneous fat and adiponectin, with a concurrent l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00213
更新日期:2017-09-01 00:00:00
abstract::Impaired regulation of hepatic glucose production is a characteristic feature of the metabolic syndrome, a cluster of diseases that includes obesity, insulin resistance, type 2 diabetes, and cardiovascular disease. It has been proposed that sustained endoplasmic reticulum stress, which appears to occur in obesity and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1014
更新日期:2006-01-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::Seven adult female rhesus monkeys were laparotomized at days 22, 42, and 157 of pregnancy and blood was collected from the uterine vein and peripheral circulation. Plasma samples were analyzed for monkey chorionic gonadotropin (mCG), monkey chorionic somatomammotropin (mCS), and prolactin by radioimmunoassay. Levels o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-851
更新日期:1977-03-01 00:00:00
abstract::The purpose of this study is to establish a primary LHRH cell culture system using embryonic olfactory placode and to examine whether LHRH cells derived from olfactory placode and the migratory pathway of LHRH neurons mature in vitro. Six monkey fetuses at the ages of E34-E36 were delivered surgically and the area inc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404690
更新日期:1993-11-01 00:00:00
abstract::The purpose of these experiments was to test the hypothesis that estrogen has an anabolic effect on cancellous bone formation in long bones of growing rats. In the present studies, estrogen decreased measurements related to cancellous bone formation, including osteoblast number, fluorochrome-labeled bone perimeter, an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243320
更新日期:1993-12-01 00:00:00
abstract::The recently discovered estrogen receptor-beta (ERbeta) is expressed in rodent and human testes. To obtain insight in the physiological role of ERbeta we have investigated the cell type-specific expression pattern of ERbeta messenger RNA (mRNA) and protein in the testis of rats of various ages by in situ hybridization...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6438
更新日期:1999-01-01 00:00:00
abstract::To analyze the molecular origin of an ER variant, the truncated ER product-1, transiently expressed at the proestrus in lactotrope cells, we generated a 2.5-kb sequence of a genomic region upstream and downstream the specific sequence truncated ER product-1. Genomic Southern blot analysis showed that truncated ER prod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8392
更新日期:2001-09-01 00:00:00
abstract::Multiplication-stimulating activity (MSA), an insulin-like growth factor (IGF) (rat IGF II), binds to extracts of many tissues from fetal lambs. We now report the presence of high concentrations of a glycoprotein receptor with a high affinity for MSA-II in microsomes prepared from fetal lamb liver. The binding of radi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-982
更新日期:1985-09-01 00:00:00
abstract::Similar to insulin, osmotic shock treatment of 3T3-L1 adipocytes causes translocation of GLUT4 protein to the plasma membrane and an increase in glucose transport activity. In our study, we evaluated the effect of chronic insulin treatment on the osmotic shock signaling pathway leading to GLUT4 translocation and gluco...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7801
更新日期:2000-12-01 00:00:00
abstract::In this study the hypothesis that the LHRH neurosecretory system of the prepubertal female monkey has the capacity to function in a manner comparable to that of monkeys in more mature stages of development was tested. Using push-pull perfusion in the stalk-median eminence, effects of electrical stimulation of the medi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3014
更新日期:1990-12-01 00:00:00
abstract::Fibroblast growth factor 9 (FGF9) signal has a role in organogenesis of the mammalian testis by controlling migration of mesonephric cells to the XY gonad, but neither it nor the FGF receptors is expressed sex-specifically. Of the Sprouty genes encoding antagonists of receptor tyrosine kinases including FGFr, mSprouty...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0299
更新日期:2006-08-01 00:00:00
abstract::The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1168
更新日期:1985-03-01 00:00:00
abstract::The amount of corticosterone bound to proteins in the adenohypophysis and dorsal hippocampus was studied concurrently with the plasma ACTH concentration in 4-week adrenalectomized male rats under steady-state conditions achieved by infusing the steroid at a constant rate for 45 min. Corticosterone binding was measured...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-1-223
更新日期:1975-07-01 00:00:00
abstract::The present study used perforated-patch electrophysiology and calcium imaging in GnRH transgenic mouse lines to determine the mechanisms underlying the potent excitatory effects of kisspeptin upon GnRH neurons in the acute brain slice preparation. Kisspeptin (100 nm) depolarized (6 +/- 1 mV) and/or evoked an 87 +/- 4%...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0321
更新日期:2008-09-01 00:00:00
abstract::The importance of a central or peripheral site of action in the blockade of ovulation induced by LHRH antagonists was investigated. To determine if hCG could induce ovulation in cycling animals given antiovulatory doses of 10 or 100 micrograms [N-Ac-D-p-Cl-Phe1,D-p-Cl-Phe2,D-Trp3,D-Arg6,D-Ala10] LHRH, the antagonist w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-756
更新日期:1985-02-01 00:00:00
abstract::It is known that renin is present in fetal membranes, with the highest concentration in the chorion laeve (reflected chorion). The purpose of this study was to identify and localize renin in human chorion laeve. Indirect immunofluorescent analysis, using antiserum against pure human kidney renin, revealed a single lay...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-4-1150
更新日期:1981-10-01 00:00:00
abstract::Progesterone (P(4)) signaling is critical for pregnancy. We previously showed that immunopilin FK506 binding protein (FKBP)52 serves as a cochaperone to optimize progesterone receptor (PR) function in the uterus, and its deficiency leads to P(4) resistance in a pregnancy stage-specific and genetic background-dependent...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1035
更新日期:2012-05-01 00:00:00
abstract::The median eminence (ME), which is the common termination field for adenohypophysiotropic systems, has been shown to produce nitric oxide (NO), a signaling molecule involved in neuroendocrine secretion. Using an ex vivo technique, 17beta-estradiol exposure to ME fragments, including vascular tissues, stimulated NO rel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6484
更新日期:1999-02-01 00:00:00
abstract::The gastrointestinal tract of the pig undergoes enhanced growth as well as morphological and functional differentiation during the perinatal period. Concurrently, porcine neonates ingest physiologically significant amounts of insulin-like growth factor-I (IGF-I) via colostrum and milk. The objectives of this study wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-1125
更新日期:1990-02-01 00:00:00
abstract::Mechanisms underlying lymphocyte infiltration of the thyroid gland and orbit in Graves' disease (GD) are poorly understood. The IGF-I receptor (IGF-IR) is a newly recognized self-antigen that, when activated in GD fibroblasts by IGF-I or GD-IgGs, provokes the expression of IL-16 and RANTES (regulated upon activation, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1375
更新日期:2006-04-01 00:00:00
abstract::The study of epigenetics allows for the understanding of gene × environmental interactions and provides a mechanism by which brief internal or external environmental changes can shape lasting differences in gene function and behavior. Epigenetic processes appear to impact a wide variety of physiological processes with...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2010-0793
更新日期:2011-02-01 00:00:00
abstract::The influence of sex hormones on the immune response to foreign antigens as well as self-antigens is now recognized. In this study, we investigated the influence of gender and sex hormones on the development of antibodies to double-stranded DNA in nonautoimmune C57BL/6J mice. Immunoglobulin G (IgG) anti-dsDNA antibodi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988450
更新日期:1994-12-01 00:00:00
abstract::The effect of fibroblast growth factor (FGF) on LH-stimulated testosterone production was investigated using primary cultures of rat testicular cells. Testicular cells obtained from neonatal rats (8-9 days of age) were maintained in culture for 3 days and then challenged with LH with or without basic FGF. After 3 addi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2935
更新日期:1988-12-01 00:00:00