Abstract:
:We analyzed the mechanism by which a Graves' disease-associated C/T polymorphism in the Kozak sequence of CD40 affects CD40 expression. CD40 expression levels on B cells in individuals with CT and TT genotypes were decreased by 13.3 and 39.4%, respectively, compared with the levels in CC genotypes (P = 0.012). Similarly, Rat-2 fibroblasts transfected with T-allele cDNA expressed 32.2% less CD40 compared with their C-allele-transfected counterparts (P = 0.004). Additionally, an in vitro transcription/translation system showed that the T-allele makes 15.5% less CD40 than the C-allele (P < 0.001), demonstrating that the effect of the single-nucleotide polymorphism (SNP) on CD40 expression is at the level of translation. However, the SNP did not affect transcription, because the mRNA levels of CD40, as measured by quantitative RT-PCR, were independent of genotype. Therefore, our results may suggest that the C allele of the CD40 Kozak SNP, which is associated with Graves' disease, could predispose to disease by increasing the efficiency of translation of CD40 mRNA.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Jacobson EM,Concepcion E,Oashi T,Tomer Ydoi
10.1210/en.2004-1617subject
Has Abstractpub_date
2005-06-01 00:00:00pages
2684-91issue
6eissn
0013-7227issn
1945-7170pii
en.2004-1617journal_volume
146pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Insulin-like growth factor (IGF) action in the brain is modulated by IGF-binding proteins (IGFBPs) whose abundance can be altered by other locally expressed growth factors. However, the mechanisms involved are unclear. We here employed the neuroblastoma cell line SK-N-MC as a model to define the mechanisms involved in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.7.6771
更新日期:1999-07-01 00:00:00
abstract::Previously, we reported that testicular macrophages constitutively release tumor necrosis factor (TNF) in vitro and are unresponsive to bacterial endotoxins [lipopolysaccharides (LPS)]. These properties are not typical of other tissue macrophages. The goals of the present study were, therefore, to establish 1) if test...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275970
更新日期:1994-01-01 00:00:00
abstract::The consumption of refined sugars continues to pose a significant health risk. However, nearly nothing is known about the effects of sugar intake by healthy women on the oocyte or embryo. Using rhesus monkeys, we show that low-dose sucrose intake over a 6-month period has an impact on the oocyte with subsequent effect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1104
更新日期:2014-07-01 00:00:00
abstract::The dissociation kinetics of uterine estradiol receptor (UER) from young, middle-aged, and old mice have been studied in vitro and correlated with interactions of the steroid receptor with nuclear suspensions in a cell-free system. Furthermore, we have determined in these various age groups of mice the concentration o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-750
更新日期:1986-02-01 00:00:00
abstract::Desensitization and internalization of G protein-coupled receptors can be mediated by phosphorylation within the C-terminal tail, facilitating beta-arrestin binding and targeting the receptor for internalization. Type II GnRH receptors (GnRH-Rs) show such regulation, but type I GnRH-Rs lack C-tails and are not rapidly...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0092
更新日期:2004-08-01 00:00:00
abstract::Progesterone released by cumulus cells surrounding the egg induces a Ca2+ influx into human sperm cells via the cationic channel of sperm (CatSper) Ca2+ channel and controls multiple Ca2+-dependent responses essential for fertilization. We hypothesized that chemical UV filters may mimic the physiological action of pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1473
更新日期:2016-11-01 00:00:00
abstract::The developmental patterns of thyroid-stimulating hormone (TSH) was examined in female Long-Evans rats by radioimmunoassay of pituitary and plasma from 5 through 80 days of age. The effects of hypothyroidism on normal TSH levels were examined in animals radiothyroidectomized at birth. Control animals showed a peak in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-1-237
更新日期:1975-07-01 00:00:00
abstract::In this study, site-directed mutagenesis and biochemical strategies have been used to establish whether disulfide bonding between extracellular Cys residues contributes to the structural integrity of the GnRH receptor (GnRH-R) and, if so, to delineate the nature of the bonding patterns involved. The majority of G prot...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5233
更新日期:1997-07-01 00:00:00
abstract::The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.7689950
更新日期:1993-09-01 00:00:00
abstract::The induction of hepatic drug metabolizing enzymes alters not only the metabolism of the xenobiotic substances that induce them but also the metabolism of various endogenous hormones. The xenobiotic receptor constitutive androstane receptor (CAR) (NR1I3) mediates the well-studied induction of CYP2B genes and other dru...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1350
更新日期:2005-03-01 00:00:00
abstract::The metabolism of [3H]progesterone by the fetal calf adrenal was examined in homogenates and microsomal fractions of adrenals obtained from mid- or late gestational fetal calves. Throughout the course of gestation, there was no detectable difference in the formation of corticosterone from progesterone by adrenal homog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-3-1077
更新日期:1983-09-01 00:00:00
abstract::The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3151
更新日期:1990-12-01 00:00:00
abstract::Previous studies have shown that increased sodium chloride in drinking water failed to suppress serum renin activity (SRA) in the etheranesthetized rat. In the absence of anesthesia, sodium chloride decreased SRA by 76% and serum aldosterone by 72%. Deoxycorticosterone acetate, when given alone, produced similar resul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-6-1394
更新日期:1975-12-01 00:00:00
abstract::In a previous study we found that PTH stimulated bone resorption and release of insulin-like growth factor-I (IGF-I) and IGF-II from cultured neonatal mouse calvaria. Since IGF-I and IGF-II stimulate osteoblast proliferation and collagen synthesis, these results suggested that increased release of IGFs during resorpti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1511
更新日期:1991-03-01 00:00:00
abstract::The present study investigated whether activation of the hexosamine biosynthesis pathway might mediate at least in part the high glucose effect on angiotensinogen (ANG) gene expression and immortalized renal proximal tubular cell (IRPTC) hypertrophy. IRPTC were cultured in monolayer. ANG, renin, and beta-actin mRNA ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0220
更新日期:2003-10-01 00:00:00
abstract::The effect of fibroblast growth factor (FGF) on LH-stimulated testosterone production was investigated using primary cultures of rat testicular cells. Testicular cells obtained from neonatal rats (8-9 days of age) were maintained in culture for 3 days and then challenged with LH with or without basic FGF. After 3 addi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2935
更新日期:1988-12-01 00:00:00
abstract::The purpose of these experiments was to determine if the powerful effect of sodium chloride concentration on angiotensin II- and potassium-stimulated aldosterone secretion by isolated perfused adrenal glands is mediated by the sodium or chloride ion or by the obligatory change in osmolality. We used isolated Ringer's ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1621
更新日期:1985-04-01 00:00:00
abstract::Because there are sex differences in feeding behavior in rats, we looked for a possible sex difference in the response to glucose of melanin-concentrating hormone (MCH) neurons in the lateral hypothalamic area using phosphorylated cAMP response element-binding protein (pCREB) as a marker of neural activity. Intact mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0078
更新日期:2005-08-01 00:00:00
abstract::In the ovarian follicle, oocyte-secreted factors induce cumulus-specific genes and repress mural granulosa cell specific genes to establish these functionally distinct cell lineages. The mechanism establishing this precise morphogenic pattern of oocyte signaling within the follicle is unknown. The present study invest...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1181
更新日期:2012-09-01 00:00:00
abstract::Glutathione (GSH) is the most abundant intracellular thiol and an important regulator of cellular redox status. Mice that lack the modifier subunit of glutamate cysteine ligase (Gclm), the rate-limiting enzyme in GSH synthesis, have decreased GSH synthesis. Nicotinamide nucleotide transhydrogenase, an inner mitochondr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0207
更新日期:2011-07-01 00:00:00
abstract::The time sequence of the metabolism of [4-14C] pregnenolone to testosterone in homogenates of human and rat testis was studied with special emphasis on the chain of events in the early 15 min of incubation. The incubations were performed at 32 C in the presence of NAD and a NADPH-generating system. The various interme...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1909
更新日期:1987-05-01 00:00:00
abstract::The pregnancy hormone relaxin has recently been shown to be cardio-protective. Despite its well-established antifibrotic actions in the heart, the effects of relaxin on cardiomyocytes (CM) remain to be determined. We investigated effects of isoform 2 of the human relaxin (H2-relaxin) on CM hypertrophy and apoptosis. I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1324
更新日期:2007-04-01 00:00:00
abstract::The effect of constant infusions of bovine TSH in different concentrations on the pattern of T4 and T3 secretion was examined in perfused canine thyroids isolated in situ. Each of the thyroid lobes was isolated and perfused in each animal. It was thus possible to examine the effect of two different concentrations of T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-656
更新日期:1977-03-01 00:00:00
abstract::MRL-lpr/lpr mice manifest a systemic lupus-like autoimmune disease. As part of this syndrome, the mice spontaneously develop autoimmune thyroiditis, which is morphologically and biochemically similar to human autoimmune thyroiditis. In this study we investigated whether thyroid tissue obtained from sites of chronic in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628400
更新日期:1995-08-01 00:00:00
abstract::The nonpeptide CRH antagonist antalarmin has been shown to block both behavioral and endocrine responses to CRH. However, it's potential activity in blunting behavioral and endocrine sequelae of stressor exposure has not been assessed. Because antagonism of central CRH by alpha-helical CRH attenuates conditioned fear ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6415
更新日期:1999-01-01 00:00:00
abstract::The extent thyroid hormone receptors (TRs) bind to AGGTCA-related motifs as monomers and/or homodimers, and as heterodimers with retinoid X receptors (RXRs) depends on the number, spacing, and orientation of these half-sites. Here we show that recombinant RXR alpha affects TR binding to DNA in diverse ways; it enhance...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7956930
更新日期:1994-11-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP)-I has been reported to inhibit gastric acid secretion through stimulation of gastric somatostatin-14 (S-14) release. To establish whether some of the effects of CGRP-I on intestinal function might also be mediated through somatostatin, fetal rat intestinal cultures were treated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.7902270
更新日期:1993-12-01 00:00:00
abstract::We have reported that calcitonin (CT) treatment induced downregulation of the CT receptor (CTR) in mouse osteoclast-like cells (OCLs). Here, we studied the features of homologous down-regulation of the CTR in mature mouse OCLs. Treatment with salmon CT (sCT) and human CT (hCT) reduced [125I]sCT specific binding. The d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536630
更新日期:1996-01-01 00:00:00
abstract::Iodine deficiency (ID) induces TSH-independent microvascular activation in the thyroid via the reactive oxygen species/nitric oxide-hypoxia-inducible factor-1α/vascular endothelial growth factor (VEGF) pathway. We hypothesized the additional involvement of mammalian target of rapamycin (mTOR) as a positive regulator o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1911
更新日期:2016-06-01 00:00:00
abstract::Characterization of glycoconjugates synthesized by polarized immature rabbit uterine epithelial (UE) cells in vitro, their vectorial patterns of secretion, and regulation by ovarian steroid hormones are reported. Large (mol wt, greater than 230 kDa) sialomucoglycoproteins and hyaluronate were primarily (86-96%) secret...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1727700
更新日期:1992-01-01 00:00:00