Abstract:
:Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effect. In both the absence and presence of insulin, there was a linear relationship between PLMT activity and lipolysis. PLMT activity was also increased in response to oxytocin, which does not activate adenylate cyclase in adipocytes and does not stimulate lipolysis. The effects of oxytocin were inhibited by insulin and were additive with those of isoproterenol on PLMT. These data support the hypothesis that in adipocytes, PLMT is activated by a cAMP-dependent protein kinase and a cAMP-independent mechanism, both of which can be regulated independently, and both of which are sensitive to inhibition by insulin.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Kelly KL,Wong EHdoi
10.1210/endo-120-6-2421subject
Has Abstractpub_date
1987-06-01 00:00:00pages
2421-7issue
6eissn
0013-7227issn
1945-7170journal_volume
120pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Neurotensin (NT) is a peptide expressed in the brain and in the gastrointestinal tract. Brain NT inhibits food intake, but the effects of peripheral NT are less investigated. In this study, peripheral NT decreased food intake in both mice and rats, which was abolished by a NT antagonist. Using c-Fos immunohistochemist...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1329
更新日期:2016-09-01 00:00:00
abstract::A basic somatomedin preparation results in a 2-fold stimulation of incorporation of both [3H]leucine into protein and 35SO4 into glycosaminoglycans by cultured chick sternal chondrocytes. The stimulation is seen in the presence of 20 mM 4-methylumbelliferyl-beta-D-xyloside alone but is not observable in the presence o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-3-744
更新日期:1980-03-01 00:00:00
abstract::Somatostatin (SRIF) inhibits vasoactive intestinal peptide (VIP)-stimulated cAMP accumulation in the GH4C1 strain of rat pituitary tumor cells, and this effect is responsible for SRIF inhibition of VIP-stimulated hormone release. In this study we examined the interaction between the SRIF receptor and adenylate cyclase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1784
更新日期:1984-05-01 00:00:00
abstract::This study was undertaken to identify uterine insulin-like growth factor-I (IGF-I) receptors and examine the regulation of these receptors throughout the estrous cycle and after 17 beta-estradiol (E2) administration to immature rats. We have demonstrated type I IGF receptors in crude uterine membranes by binding and c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-597
更新日期:1989-08-01 00:00:00
abstract::It is generally accepted that cholesterol affects dynamic membrane properties and the function of membrane bound proteins involved in secretion processes. In the present study we employed GH4C1 cells treated with human lipoprotein-deficient serum (h-LPDS), exogenous cholesterol and high density lipoprotein (HDL3) to i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.4.8462470
更新日期:1993-04-01 00:00:00
abstract::The effects of endothelin-1 (ET-1), a potent vasoconstrictor and mitogen to various cell types, on proliferation and differentiated functions of the murine Leydig tumor cell line MA-10 were investigated. Kinetic binding experiments at room temperature showed that [125I] ET-1 bound to MA-10 cells and reached equilibriu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425480
更新日期:1993-02-01 00:00:00
abstract::The existence of renin in a number of extrarenal tissues has been well documented, but the physiological role of extrarenal renin remains unknown. To study the physiological role of adrenal renin, we developed a serum-free culture system for adrenal capsular/zona glomrulosa explants. Explants showed good viability in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-486
更新日期:1989-07-01 00:00:00
abstract::The effect of collagenase dissociation of virgin mouse mammary glands on the level of mammary epithelial cytosolic estrogen receptors (ER) and progesterone receptors (PR) was assessed. After cell dissociation, ER was present in mammary epithelial cells at concentrations similar to those found in the whole gland. Howev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-105
更新日期:1985-01-01 00:00:00
abstract::Tobacco smoking favorably influences the course of Hashimoto thyroiditis, possibly through the antiinflammatory proprieties of nicotine. In this study we tested anatabine, another tobacco alkaloid, in a model of experimental autoimmune thyroiditis. Experimental autoimmune thyroiditis was induced by different doses of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1452
更新日期:2012-09-01 00:00:00
abstract::The possibility that chronic, multigenerational exposure to environmental estrogens selects for adaptive hormone-response phenotypes is a critical unanswered question. Embryos/larvae of killifish from an estrogenic-polluted environment (New Bedford Harbor, MA [NBH]) compared with those from a reference site overexpres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1052
更新日期:2016-06-01 00:00:00
abstract::An immunomodulatory role for LHRH was suggested when we reported the presence of immunoactive and bioactive LHRH and its mRNA in rat splenic and thymic lymphocytes. In this paper we report that human peripheral T-cells as well as its subsets CD4+ and CD8+ contained immunoactive and bioactive LHRH. Furthermore, analysi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8319570
更新日期:1993-07-01 00:00:00
abstract::Recent studies have demonstrated that passive immunization of neonatal rats to GRF inhibited their somatic growth through the suppression of GH secretion. In this study, we investigated the changes in pituitary GRF receptor (GRFR) expression in GRF antibody (GRF-ab) treated rats. Neonatal rats were treated from day 1 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641220
更新日期:1996-06-01 00:00:00
abstract::The mechanisms whereby tissue sensitivity to PRL is controlled are not well understood. Here we report that expression of mRNA and protein for members of the SOCS/CIS/JAB family of cytokine signaling inhibitors is increased by PRL administration in ovary and adrenal gland of the lactating rat deprived of circulating P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.11.8466
更新日期:2001-11-01 00:00:00
abstract::In agonadal juvenile male monkeys, continuous administration of human metastin 45-54 (hu metastin 45-54) leads to desensitization of its receptor, G protein-coupled receptor 54 (GPR54), and decreased LH. The present study extended this observation to the adult male monkey, a more preclinically relevant model in which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0207
更新日期:2007-07-01 00:00:00
abstract::The effects of electrical stimulation of the brain on the ovarian venous plasma concentrations of estradiol (E2) and progesterone (P) were investigated in female rats hypophysectomized and adrenalectomized at 1000 h on the day of proestrus. Stimulation was applied during the proestrous critical period under pentobarbi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-136
更新日期:1981-07-01 00:00:00
abstract::The direct actions of kit-ligand/stem cell factor (KL) in developing ovarian follicles were investigated. Previous studies have shown that granulosa cells express KL that can support oocyte development. The current study demonstrates that KL can also act directly on theca cells to promote cellular growth and different...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5368
更新日期:1997-09-01 00:00:00
abstract::During lung development there is tension between positive and negative regulators of fibroblast-epithelial communication controlling type II cell differentiation. A clinical consequence of imbalance of this tension is the increased risk for respiratory distress syndrome in male infants. We hypothesized that chronic in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.8.7615
更新日期:2000-08-01 00:00:00
abstract::Recent studies from our laboratory indicate that high density lipoproteins (HDL) and apolipoproteins-AI, -AII, and -CI stimulate the release of human placental lactogen (hPL) from human placental explants and trophoblast cells. However, the intracellular mechanisms by which HDL stimulate hPL release are unknown. Since...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-4-1879
更新日期:1988-10-01 00:00:00
abstract::Experimental evidence indicates that the neonatal gonadal steroid environment is an important determinant of the sexual dimorphism of GH secretion and body growth. However, the influence of the sex steroids in GH control during adult life and their mechanism/site of action are largely unknown. In the present study we ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1345780
更新日期:1992-01-01 00:00:00
abstract::RNA blot hybridization analysis with cloned rat CRF precursor (prepro-CRF) cDNA as a probe showed that prepro-CRF mRNA existed in rat hypothalamic and extrahypothalamic brain tissue, whereas it was undetectable in the pituitary and adrenal. To study the effect of glucocorticoid on the level of prepro-CRF mRNA in the h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1314
更新日期:1985-10-01 00:00:00
abstract::Although the Sertoli cell is a key cell mediating the actions of FSH- and LH-stimulated testosterone (T) in the testis, there is little information to indicate how this cell responds structurally to hormonal insufficiency. The present study used morphometric techniques to study the structural manifestations of the Ser...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.1.1612030
更新日期:1992-07-01 00:00:00
abstract::The effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the 5'-deiodinase (5'D) activity was studied in rat astroglial cells cultured in chemically defined medium. TPA promoted a large increase in the type II 5'D activity, which was maximal 5-10 h after addition of TPA and then d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1277
更新日期:1989-09-01 00:00:00
abstract::Ghrelin is produced by the gastrointestinal tract, and its systemic concentrations are mainly regulated by nutritional factors. Our aim was to investigate: 1) endogenous portal and systemic acylated and unacylated ghrelin levels (AG and UAG, respectively); 2) whether an iv glucose tolerance test (IVGTT) modifies AG an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0225
更新日期:2007-11-01 00:00:00
abstract::Cysteamine [2-aminoethanethiol (CySH)] reduces measurable PRL concentrations in vivo and in vitro. Since secretion is also inhibited, CySH may block conversion from a poorly assayable hormone storage form(s) to readily assayable, releasable PRL. This would represent a previously unrecognized mechanism for secretory re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1492
更新日期:1984-10-01 00:00:00
abstract::The response of fat tissue to GH or insulin-like growth factor I (IGF-I) differs between humans with hypopituitarism and those with exogenous obesity; the effects of combined GH and IGF-I administration have not been compared in these two situations. In GH-deficient dwarf rats (who have a primary GH deficiency), the e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.7.8770900
更新日期:1996-07-01 00:00:00
abstract::Amplification of insulin secretion by cAMP is mediated by protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). Using selective activators, we determined how each effector influences the cytosolic free Ca(2+) concentration ([Ca(2+)]c) and insulin secretion in mouse islets. Alone PKA activator ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1247
更新日期:2014-09-01 00:00:00
abstract::Continuously superfused rat anterior pituitary cells were used to study the effects of prostaglandins (PGs) and a thromboxane (TX) on the secretion of TSH. Indomethacin, a blocker of PG synthetase, inhibited the amount of TSH secreted in response to TRH. This reduction in TRH responsiveness was overcome by administrat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-637
更新日期:1981-08-01 00:00:00
abstract::The occurrence of the thyroid hormone-binding plasma protein transthyretin in the bloodstream was investigated for four American marsupial species. Serum samples were analyzed by incubation with radioactive T4, followed by electrophoresis, then autoradiography, and Western blotting. Transthyretin was found in serum fr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754780
更新日期:1996-08-01 00:00:00
abstract::The uptake of [125I]triiodothyroacetic acid ([125I]Triac) in anterior pituitary cells was investigated and compared with that of [125I]T3. Furthermore, the effects of Triac, T3, and T4 on TSH release were compared. Cells isolated from adult male Wistar rats were cultured for 3 days in medium with 10% fetal calf serum....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988460
更新日期:1994-12-01 00:00:00
abstract::PTH-related protein (PTHrP), the major mediator of hypercalcemia of malignancy, reduces tubular phosphate (Pi) reabsorption through its PTH-like renotropic actions. Another peptide detected in tumoral cells, transforming growth factor-beta (TGF beta), has been shown to considerably suppress the sodium-dependent Pi tra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8391420
更新日期:1993-07-01 00:00:00