Cholesterol cell content affects prolactin but not growth hormone release in GH4C1 cells.

abstract：:Female Chinese hamsters (n = 10) were immunized with Chinese hamster ovary (CHO) cells that expressed the human TSH receptor (TSHR) to generate a model of Graves' disease. TSHR-autoantibodies (TSHR-Ab) were determined by CHO-TSHR. Two hamsters with stimulating TSHR-Ab showed thyrocyte hypertrophy associated with a foc...

journal_title：Endocrinology

authors： Ando T,Imaizumi M,Graves P,Unger P,Davies TF

更新日期：2003-02-01 00:00:00

abstract：:The Src homology 2-containing tyrosine phosphatase, Shp-2, is a crucial enzyme that mediates intracellular signaling and is implicated in cell proliferation and differentiation. Here we investigated the involvement of the Shp-2 tyrosine phosphatase in determining the downstream signaling pathways initiated by the Ret ...

journal_title：Endocrinology

authors： D'Alessio A,Califano D,Incoronato M,Santelli G,Florio T,Schettini G,Carlomagno MS,Cerchia L,de Franciscis V

更新日期：2003-10-01 00:00:00

abstract：:Studies were carried out to determine the origin of the immediate increase in plasma calcium following acute calcitonin deficiency in mature rats. Animals were pre-labelled with 45calcium 24 hours and 4 weeks before thyroparathyroidectomy (TPTX) and 1 hour, 24 hours, 2 and 4 weeks before nephrectomy and TPTX and bled ...

journal_title：Endocrinology

authors： Kalu DN,Hadji-Georgopoulos A,Foster GV

更新日期：1976-02-01 00:00:00

abstract：:Daily treatment with 1 mg of the GnRH antagonist [Ac-delta 3-Pro1,pC1-D-Phe2,D-Trp3,6,N alpha MeLeu7]-GnRH abolished mating behavior and disrupted spermatogenesis in male rats within 2 weeks. This was accompanied by a sharp decrease in plasma testosterone (T) levels as well as sexual organ weights while body weight re...

journal_title：Endocrinology

authors： Rivier C,Rivier J,Vale W

更新日期：1981-05-01 00:00:00

abstract：:Oxytocin (OT) has a dual action in the uterus: a uterotonic action on myometrial cells and a prostaglandin (PG)-releasing action on endometrial/decidual cells. It had not been determined whether the OT-binding sites or receptors on the myometrial and the endometrial/decidual membranes are of the same type or may repre...

journal_title：Endocrinology

authors： Chan WY,Chen DL,Manning M

更新日期：1993-03-01 00:00:00

abstract：:The availability of a mitogenic bioassay for PRL in which multiple, small samples could be analyzed led us to examine circulating and pituitary PRL bioactivity during the estrous cycle of the rat. Bio- (B) and immuno (I)-activities were compared in individual samples using rat PRL RP-1 standard. B:I ratios ranged betw...

journal_title：Endocrinology

authors： Blank MS,Ching MC,Dufau ML

更新日期：1986-05-01 00:00:00

abstract：:The conceptus and endometrium secrete large amounts of prostaglandin E₂ (PGE₂) into the porcine uterine lumen during the periimplantation period. We hypothesized that PGE₂ acts on conceptus/trophoblast cells through auto- and paracrine mechanisms. Real-time RT-PCR analysis revealed that PGE₂ receptor (PTGER)2 mRNA was...

journal_title：Endocrinology

authors： Waclawik A,Kaczynski P,Jabbour HN

更新日期：2013-10-01 00:00:00

abstract：:The effect of constant infusions of bovine TSH in different concentrations on the pattern of T4 and T3 secretion was examined in perfused canine thyroids isolated in situ. Each of the thyroid lobes was isolated and perfused in each animal. It was thus possible to examine the effect of two different concentrations of T...

journal_title：Endocrinology

authors： Laurberg P

更新日期：1977-03-01 00:00:00

abstract：:In healthy individuals, peripheral insulin resistance evoked by dietary saturated lipid can be accompanied by increased insulin secretion such that glucose tolerance is maintained. Substitution of long-chain omega-3 fatty acids for a small percentage of dietary saturated fat prevents insulin resistance in response to ...

journal_title：Endocrinology

authors： Holness MJ,Greenwood GK,Smith ND,Sugden MC

更新日期：2003-09-01 00:00:00

abstract：:Brain-derived neurotropic factor (BDNF) is a member of the nerve growth factor family that is important for neuronal survival and plasticity. We recently demonstrated that stress decreases BDNF messenger RNA (mRNA) levels in the hippocampus, which raises the possibility that BDNF may play a role in regulation of the h...

journal_title：Endocrinology

authors： Smith MA,Makino S,Kim SY,Kvetnansky R

更新日期：1995-09-01 00:00:00

abstract：:Previous studies have shown that aldosterone plus salt loading cause cardiac hypertrophy in rats in vivo, and that in vitro, both aldosterone and glucose stimulate fibroblast growth. The present studies examined the effects of adrenal steroids via mineralocorticoid and glucocorticoid receptors (MR and GR) on [3H]leuci...

journal_title：Endocrinology

authors： Sato A,Funder JW

更新日期：1996-10-01 00:00:00

abstract：:The effect of osmotic changes on aldosterone production, [Ca2+]i and voltage-gated Ca2+ currents, was studied in cultured rat glomerulosa cells. Alteration of osmolarity by sucrose addition in the 250-330 mosM range did not influence aldosterone production per se, but it substantially affected K+-stimulated aldosteron...

journal_title：Endocrinology

authors： Makara JK,Petheö GL,Tóth A,Spät A

更新日期：2000-05-01 00:00:00

abstract：:The antigenic epitopes of human GH (hGH) have been investigated using monoclonal antibodies (Mabs) to hGH. A panel of 12 Mabs was incorporated into two-site binding assays in order to investigate the antigenic surface of hGH. Nine reaction patterns were observed. The Mabs were further characterized in terms of their c...

journal_title：Endocrinology

authors： Strasburger CJ,Kostyo J,Vogel T,Barnard GJ,Kohen F

更新日期：1989-03-01 00:00:00

abstract：:Recent studies have indicated that growth factors such as insulin-like growth factors (IGFs) increase the growth rate of cultured preimplantation embryos. We therefore hypothesized that the fallopian tube may produce IGFs which in turn participate in the regulation of preimplantation embryo development in vivo. In the...

journal_title：Endocrinology

authors： Carlsson B,Hillensjö T,Nilsson A,Törnell J,Billig H

更新日期：1993-11-01 00:00:00

abstract：:To obtain large amounts of hTSH and to study the role of the N-linked oligosaccharides for its biological activity, hTSH was produced using recombinant baculovirus containing the human alpha-subunit and a hTSH beta-minigene, respectively, both under the control of the polyhedrin promoter. Expression in insect cells wa...

journal_title：Endocrinology

authors： Grossmann M,Wong R,Teh NG,Tropea JE,East-Palmer J,Weintraub BD,Szkudlinski MW

更新日期：1997-01-01 00:00:00

abstract：:The structural requirements for the inhibition of net bone collagen synthesis by parathyroid hormone (PTH) in vitro have been examined by study of the effects of selected fragments and analogs of bovine PTH (bPTH) upon the incorporation of [3H]proline into collagenase-digestible and -nondigestible proteins by neonatal...

journal_title：Endocrinology

authors： Bringhurst FR,Potts JT Jr

更新日期：1981-01-01 00:00:00

abstract：:Biological actions of natriuretic peptide (NP) are determined by the condition of the receptor as well as that of the hormone. Although we previously demonstrated in hypertensive rats the up-regulation of NP-A receptor that mediates various biological actions of NPs, the pathophysiologic significance of NP-C receptor,...

journal_title：Endocrinology

authors： Yoshimoto T,Naruse M,Naruse K,Shionoya K,Tanaka M,Tanabe A,Hagiwara H,Hirose S,Muraki T,Demura H

更新日期：1996-03-01 00:00:00

abstract：:Mice with the recessively inherited obese-hyperglycemic syndrome (ob/ob) and their nonobese litter mates were studied over a 26-week period. The body weights and serum glucose levels of ob/ob mice began to rise markedly at 5-6 weeks of age and remained elevated throughout the period of study. Obese mice were significa...

journal_title：Endocrinology

authors： Larson BA,Sinha YN,Vanderlaan WP

更新日期：1976-01-01 00:00:00

abstract：:We recently reported that fetal sheep corticotrophs (ACTH-producing cells) at 108 +/- 5 d (days) of gestation are relatively more responsive to CRH than to AVP, whereas those at 139 +/- 0 d (term = 145 d) and in the adult are more responsive to AVP. To further characterize these developmental changes, we used immunocy...

journal_title：Endocrinology

authors： Perez FM,Schwartz J,Rose JC

更新日期：1997-03-01 00:00:00

abstract：:A central step in the development and sexual differentiation of the brain is the intraneuronal conversion of testosterone to estrogen. This conversion is catalyzed by an enzyme complex comprised of cytochrome P450 aromatase and the ubiquitous NADPH-dependent cytochrome P450 reductase. Brain aromatase activity is devel...

journal_title：Endocrinology

authors： Lauber ME,Lichtensteiger W

更新日期：1994-10-01 00:00:00

abstract：:Quantitative thyroid cell transplantation was used to evaluate the reestablishment of thyroid function in surgically thyroidectomized rats. Donor thyroid glands were enzymatically dispersed, and the cells were quantified, serially diluted, and inoculated into the sc fat pads of syngeneic recipient rats 1 day after sur...

journal_title：Endocrinology

authors： Domann FE,Mitchen JM,Clifton KH

更新日期：1990-12-01 00:00:00

abstract：:We have investigated the role of protein kinase C (PKC) isoenzymes in the differential growth regulation of human pancreatic carcinoma cell lines by all-trans retinoic acid (RA). RA treatment results in dose-dependent stimulation of anchorage-independent growth in AsPc1 cells and growth inhibition in Capan 2 cells. Bo...

journal_title：Endocrinology

authors： Rosewicz S,Brembeck F,Kaiser A,Marschall ZV,Riecken EO

更新日期：1996-08-01 00:00:00

abstract：:Insulin is a potent regulator of Sp1 transcription factor. To examine if glucagon, which usually antagonizes insulin, regulates Sp1, we assessed the levels of Sp1 by Western blotting from H-411E cells exposed to glucagon with or without insulin. Glucagon alone (1.5 x 10(-9) to 1.5 x 10(-5) M) stimulated Sp1 accumulati...

journal_title：Endocrinology

authors： Keembiyehetty CN,Candelaria RP,Majumdar G,Raghow R,Martinez-Hernandez A,Solomon SS

更新日期：2002-04-01 00:00:00

abstract：:Canine renal cortical PTH receptors were solubilized after occupancy of membrane-associated receptors with the agonist ligand [125I]bovine (b) PTH-(1-34). Stabilization of binding during solubilization required the use of high concentrations of BSA (optimally 5%) and appropriate detergents (0.5% 3-[(3-cholamidopropyl)...

journal_title：Endocrinology

authors： Nissenson RA,Mann E,Winer J,Teitelbaum AP,Arnaud CD

更新日期：1986-03-01 00:00:00

abstract：:Although renal hypertrophy occurs rapidly after uninephrectomy, restoring the majority of renal excretory function, it remains unknown whether similar compensatory mechanisms maintain 1,25-dihydroxyvitamin D production (and calcium homeostasis). To address this issue we compared plasma calcitriol levels and renal 25-h...

journal_title：Endocrinology

authors： Almond JR,Bales CW,Lobaugh B,Klotman PE,Drezner MK

更新日期：1989-05-01 00:00:00

abstract：:L-700,653 is a potent nonpeptidyl GH secretagogue consisting of a benzolactam structure: (4'-[[3(R)-[[3-[(2(S),3-dihydroxypropyl) amino]3-methyl-1-oxobutyl]amino]2,3,4,5-tetrahydro-2-oxo-1H-1-benzaze pin- 1-yl]methyl][1,1'-biphenyl]2-carboxamide hydrochloride). When administered sc by a Medi-Jector device at 0, 0.003,...

journal_title：Endocrinology

authors： Chang CH,Rickes EL,Marsilio F,McGuire L,Cosgrove S,Taylor J,Chen H,Feighner S,Clark JN,De Vita R

更新日期：1995-03-01 00:00:00

abstract：:The somatic Sertoli cells of the testis are major targets for FSH and are important for the regulation of spermatogenesis. The binding of FSH to Sertoli cells activates the cAMP-dependent protein kinase A signaling pathway, resulting in phosphorylation of the cAMP response element-binding protein (CREB), which is requ...

journal_title：Endocrinology

authors： Walker WH,Fucci L,Habener JF

更新日期：1995-08-01 00:00:00

abstract：:We have investigated the interaction of estrogen with GnRH on the regulation of LH subunit mRNA in female hypogonadal (hpg) mice receiving constant frequency and amplitude pulsatile GnRH treatment for up to 18 days. The level of cytosolic common alpha mRNA in female hpg mouse pituitaries was 45 +/- 6% of normal female...

journal_title：Endocrinology

authors： Saade G,London DR,Clayton RN

更新日期：1989-04-01 00:00:00

abstract：:Although factors that regulate cAMP and steroid production in granulosa cells of hen preovulatory follicles have been well studied, much less is known of the mechanisms that control steroidogenesis in the adjacent thecal layer. These studies were conducted to examine the involvement and interaction of cAMP and protein...

journal_title：Endocrinology

authors： Tilly JL,Johnson AL

更新日期：1989-09-01 00:00:00

abstract：:GH controls its own secretion through a mechanism involving short-loop feedback regulation of the synthesis and release of GH-releasing hormone (GHRH). GHRH neurons coexpress the peptide galanin, but the functional significance of this coexpression is unknown. In this study, we tested the hypotheses that 1) galanin ge...

journal_title：Endocrinology

authors： Chan YY,Grafstein-Dunn E,Delemarre-van de Waal HA,Burton KA,Clifton DK,Steiner RA

更新日期：1996-12-01 00:00:00