Abstract:
:The occurrence of the thyroid hormone-binding plasma protein transthyretin in the bloodstream was investigated for four American marsupial species. Serum samples were analyzed by incubation with radioactive T4, followed by electrophoresis, then autoradiography, and Western blotting. Transthyretin was found in serum from Monodelphis domestica, Didelphis virginiana, Caluromys lanatus, and Dromiciops australis. For unambiguous identification, transthyretin from D, virginiana was purified from serum and its N-terminal amino acid sequence was determined. The obtained results suggest that the initiation of transthyretin gene expression in the liver of marsupials occurred independently in several lineages of American marsupials, all of which are at the ends of phylogenetic branches. The expression of the transthyretin gene in the liver of the American polyprotodont marsupials contrasts with the lack of transthyretin gene expression in the liver of all 22 previously investigated Australian Polyprotodonta.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Richardson SJ,Wettenhall RE,Schreiber Gdoi
10.1210/endo.137.8.8754780subject
Has Abstractpub_date
1996-08-01 00:00:00pages
3507-12issue
8eissn
0013-7227issn
1945-7170journal_volume
137pub_type
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