Abstract:
:Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells. Death factors such as Fas ligand induce apoptosis by triggering a series of intracellular protein-protein interactions mediated by defined motifs found in the signaling molecules. One of these motifs is the death effector domain (DED), a stretch of about 80 amino acids that is shared by adaptors, regulators, and executors of the death factor pathway. We have identified the human and rat complementary DNAs encoding a novel protein termed DEFT (Death EFfector domain-containing Testicular molecule). The N-terminus of DEFT shows a high degree of homology to the DEDs found in FADD (an adaptor molecule) as well as procaspase-8/FLICE and procaspase-10/Mch4 (executors of the death program). Northern blot hybridization experiments have shown that the DEFT messenger RNA (mRNA) is expressed in a variety of human and rat tissues, with particularly abundant expression in the testis. In situ hybridization analysis further indicated the expression of DEFT mRNA in meiotic male germ cells. In a model of germ cell apoptosis induction, an increase in testis DEFT mRNA was found in immature rats after 2 days of treatment with a GnRH antagonist. Unlike FADD and procaspase-8/FLICE, overexpression of DEFT did not induce apoptosis in Chinese hamster ovary cells. Although cotransfection studies indicated that DEFT is incapable of modulating apoptosis effected by FADD and procaspase-8/FLICE, interactions between DEFT and uncharacterized DED-containing molecules in the testis remain to be studied in the future. In conclusion, we have identified a novel DED-containing protein with high expression in testis germ cells. This protein may be important in the regulation of death factor-induced apoptosis in the testis and other tissues.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Leo CP,Hsu SY,McGee EA,Salanova M,Hsueh AJdoi
10.1210/endo.139.12.6335subject
Has Abstractpub_date
1998-12-01 00:00:00pages
4839-48issue
12eissn
0013-7227issn
1945-7170journal_volume
139pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::CRF dose-dependently enhanced corticosterone (B) secretion by rat adrenal slices including both cortex and medulla. Conversely, CRF did not exert any B response by fragments of adrenocortical autotransplants, which are completely deprived of chromaffin tissue. However, autotransplant quarters exhibited a dose-dependen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.1.1319330
更新日期:1992-07-01 00:00:00
abstract::The objective of these experiments was to investigate the influence of activation of three second messenger systems (protein kinase-C, adenylate cyclase-cAMP, and calcium mobilization) on the secretion of pancreastatin (PST) and chromogranin-A (CGA) by a human pancreatic carcinoid cell line (BON) in tissue culture. St...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-220
更新日期:1991-01-01 00:00:00
abstract::Clinically, approximately 30% of women who receive synthetic glucocorticoids (sGC) for risk of preterm labor carry to term. In vitro studies have shown that sGC acutely regulate the placental system A amino acid transporter, but there are no comparable data in vivo. Hence, the objective of our study was to examine the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0104
更新日期:2011-09-01 00:00:00
abstract::In developed countries, the increasing incidence of obesity is a serious health problem. Leptin exposure in the perinatal period affects long-term regulation of appetite and energy expenditure, but control of leptin production in utero is unclear. This study investigated perirenal adipose tissue (PAT) and placental le...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0310
更新日期:2007-08-01 00:00:00
abstract::Thyroid hormone (T3) is required for postnatal skeletal growth. It exerts its effect by binding to nuclear receptors, TRs including TRα1 and TRβ1, which are present in most cell types. These cell types include chondrocytes and osteoblasts, the interactions of which are known to regulate endochondral bone formation. In...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1109
更新日期:2014-08-01 00:00:00
abstract::To explore the role of transforming growth factor-beta (TGF beta) isoforms and other growth-related genes during prostate morphogenesis in the mouse, we examined mRNA levels in fetal day 17 urogenital sinus, mesenchyme (UGM), and epithelium (UGE) as well as in the ventral, dorsal, and anterior lobes of the adult prost...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119140
更新日期:1994-03-01 00:00:00
abstract::The availability of a mitogenic bioassay for PRL in which multiple, small samples could be analyzed led us to examine circulating and pituitary PRL bioactivity during the estrous cycle of the rat. Bio- (B) and immuno (I)-activities were compared in individual samples using rat PRL RP-1 standard. B:I ratios ranged betw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-5-1886
更新日期:1986-05-01 00:00:00
abstract::Dopamine D2 receptor agonists are commonly used in the control of PRL-secreting adenomas, and the sensitivity of dopamine agonists during long term therapy is exquisite. However, the molecular mechanisms responsible for the maintenance of this cellular sensitivity to dopamine agonists remain poorly understood. In the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5433
更新日期:1997-10-01 00:00:00
abstract::A protein [steroidogenesis-inducing protein (SIP)] has been isolated from human ovarian follicular fluid and shown previously to stimulate steroidogenesis in Leydig cells, adrenal cells, and early luteal cells. Since proteins and peptides known to regulate steroidogenesis, such as gonadotropins and growth factors, als...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1733710
更新日期:1992-02-01 00:00:00
abstract::The action of sex steroids on the growth and differentiation of target tissues requires the presence of specific intracellular receptors. We compared the distribution of cells containing estrogen receptor (ER) and/or progesterone receptor (PR) in rabbit uterus by immunohistochemistry using monoclonal antibodies direct...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2728
更新日期:1989-11-01 00:00:00
abstract::A complementary DNA, uterine-ovarian-specific gene 44 (UO-44), has been isolated from tamoxifen-induced rat uterine complementary DNA library using differential display techniques. UO-44 transcripts are found to be abundant in the uterus and ovary. UO-44 gene expression in the uterus is strictly regulated by estrogens...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8247
更新日期:2001-07-01 00:00:00
abstract::Exposure of quiescent bovine parathyroid cells to serum or a serum substitute caused an elevation in [3H] thymidine incorporation, followed by an increase in cell number. This was preceded by a rapid transient rise in c-myc and c-fos proto-oncogene mRNA levels. Alterations in the medium calcium concentration had no ef...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-935
更新日期:1989-08-01 00:00:00
abstract::Dispersed pancreatic islet cells were analyzed for their low forward angle light scatter using flow cytometry. The cells produced a distinct light scatter pattern which appeared to be a function of cell size and not cell granularity. RIA of hormone content of cells collected from different regions of the pattern revea...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-5-1791
更新日期:1983-11-01 00:00:00
abstract::Thyroid hormones regulate bone homeostasis, and exogenously induced hyperthyroidism and hypothyroidism in mice was recently found to be associated with an altered expression of the Wnt inhibitor Dickkopf-1 (Dkk1), a determinant of bone mass. Here, we assessed the role of Dkk1 in thyroid hormone-induced changes in bone...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00998
更新日期:2019-03-01 00:00:00
abstract::Aldosterone plays a major role in regulating sodium and potassium flux in epithelial tissues such as kidney and colon. Recent evidence suggests that serum- and glucocorticoid-regulated kinase (SGK) is induced by aldosterone and acts as a key mediator of aldosterone action in epithelial tissues. Induction of SGK messen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.4.8095
更新日期:2001-04-01 00:00:00
abstract::Vasoactive intestinal polypeptide (VIP) was measured by RIA in the hypophysial portal blood of rats after total hypophysectomy. The mean (+/- SE) VIP concentrations were 1332 +/- 171 pg/ml (range, 300-3868 pg/ml) under urethane anesthesia and 1735 +/- 707 pg/ml under pentobarbital anesthesia. The concentrations of VIP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-395
更新日期:1981-02-01 00:00:00
abstract::Carboxyl-terminal fragments of PTH (C-PTH) appear to have biological properties different from those mediated by the amino-terminal portions of PTH and PTH-related peptide (PTHrP). To characterize a C-PTH receptor that may be involved in mediating these functions, we performed RRAs and affinity cross-linking studies w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588200
更新日期:1995-11-01 00:00:00
abstract::GnRH neurons active in the preovulatory LH surge have been identified in several species using the early intermediate gene product, Fos, but the GnRH neurons active during episodic LH secretion remain unknown. In this study, we have used Fos and Fos-related antigens (FRA) to determine whether a subset of GnRH neurons ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7216
更新日期:1999-12-01 00:00:00
abstract::Previous studies have shown that cysteamine [2-aminoethanethiol (CSH)], given in vivo or in vitro, rapidly but reversibly depletes immunoreactive PRL from the anterior pituitary (AP) through a mechanism which, in vitro, does not appear to involve the dopamine (DA) receptor. In the present investigation, these effects ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-6-2161
更新日期:1983-12-01 00:00:00
abstract::Reproductive fitness in females is susceptible to obesogenic diets. Energy balance and reproduction are tightly regulated, in part, by hypothalamic neurons in the arcuate nucleus (ARC), and high-fat diet (HFD) can steadily increase estradiol levels in rodents. Estradiol regulates the reproductive axis via negative fee...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa146
更新日期:2020-10-01 00:00:00
abstract::Perturbing the endoplasmic reticulum homeostasis of thyroid cell lines with thapsigargin, a specific inhibitor of the sarcoendoplasmic reticulum Ca(2+) adenosine triphosphatases, and tunicamycin, an inhibitor of the N-linked glycosylation, blocked Tg in the endoplasmic reticulum. This event was signaled outside the en...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8825
更新日期:2002-06-01 00:00:00
abstract::The levels of the GnRH receptor (GnRHR) and its messenger RNA depend on the pattern of administration of GnRH. In this study, internal deletion mutants in a luciferase reporter gene vector (GnRHR-pXP2) containing a 1226-bp promoter fragment of mouse GnRHR gene were used to examine the regulation of GnRHR gene transcri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6945
更新日期:1999-08-01 00:00:00
abstract::Sterol regulatory element binding protein 1c (SREBP1c) is a master regulator of lipogenic gene expression in liver and adipose tissue, where its expression is regulated by a heterodimer of nuclear receptor-type transcription factors retinoid X receptor-alpha (RXRalpha) and liver X receptor-alpha (LXRalpha). Despite th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1461
更新日期:2008-05-01 00:00:00
abstract::As a metabolic sensor, the serine/threonine protein kinase AMP-activated protein kinase (AMPK) promotes the adaptation of cells to signals arising from nutrients, hormones, and growth factors. The ability of IGF-I to stimulate protein synthesis is suppressed by AMPK, therefore, these studies were undertaken to determi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0155
更新日期:2011-08-01 00:00:00
abstract::Mouse fibroblast cell lines which secrete bovine (b) GH have been generated. This was accomplished by cotransforming mouse L cells (thymidine kinase-negative [TK-] and adenine phosphoribosyl transferase-negative [APRT-]) with DNA molecules encoding the Rous sarcoma virus-long-terminal repeat and bGH genes along with t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1489
更新日期:1986-10-01 00:00:00
abstract::Studies were carried out to determine the origin of the immediate increase in plasma calcium following acute calcitonin deficiency in mature rats. Animals were pre-labelled with 45calcium 24 hours and 4 weeks before thyroparathyroidectomy (TPTX) and 1 hour, 24 hours, 2 and 4 weeks before nephrectomy and TPTX and bled ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-2-534
更新日期:1976-02-01 00:00:00
abstract::Task3 K(+) channels are highly expressed in the adrenal cortex and contribute to the angiotensin II and K(+) sensitivity of aldosterone-producing glomerulosa cells. Adult Task3(-/-) mice display a partially autonomous aldosterone secretion, subclinical hyperaldosteronism, and salt-sensitive hypertension. Here, we inve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1101
更新日期:2013-08-01 00:00:00
abstract::Exposure to environmental toxicants during fetal development alters gene expression and promotes disease later in life. Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer widely used for the manufacturing of consumer products. Exposure to DEHP has been associated with obesity, asthma, and low T levels. In utero expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1436
更新日期:2015-01-01 00:00:00
abstract::To examine further the relationship between developmental changes in LH release and the onset of puberty, effects of posterior hypothalamic lesions were tested in ovariectomized (OVX), sexually immature female monkeys. In OVX females (n = 3) with sham hypothalamic lesions basal LH levels were suppressed during the pre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-445
更新日期:1988-07-01 00:00:00
abstract::The contributions of estradiol and testosterone to atherosclerotic lesion progression are not entirely understood. Cross-sex hormone therapy (XHT) for transgender individuals dramatically alters estrogen and testosterone levels and consequently could have widespread consequences for cardiovascular health. Yet, no prec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00884
更新日期:2018-02-01 00:00:00