Abstract:
:A complementary DNA, uterine-ovarian-specific gene 44 (UO-44), has been isolated from tamoxifen-induced rat uterine complementary DNA library using differential display techniques. UO-44 transcripts are found to be abundant in the uterus and ovary. UO-44 gene expression in the uterus is strictly regulated by estrogens, tamoxifen, and GH, whereas the pure antiestrogen ICI 182780 is inhibitory. Treatment of ovariectomized rats and hypophysectomized rats with tamoxifen and GH, respectively, resulted in up-regulation of UO-44 expression in a dose-dependent manner. In situ hybridization revealed that UO-44 gene expression was restricted to the luminal and glandular epithelial cells of the uterus and to granulosa cells of medium-size ovarian follicles. Transfection studies showed that UO-44 was a membrane-associated protein. Because estrogens, tamoxifen, and GH are stimulators of uterine luminal epithelial cell growth in vivo, UO-44 protein may serve as a mediator of the effect of these compounds in inducing epithelial proliferation and differentiation in these tissues.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Huynh H,Ng CY,Lim KB,Ong CK,Ong CS,Tran E,Tuyen Nguyen TT,Chan TWdoi
10.1210/endo.142.7.8247subject
Has Abstractpub_date
2001-07-01 00:00:00pages
2985-95issue
7eissn
0013-7227issn
1945-7170journal_volume
142pub_type
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