Abstract:
:Carboxyl-terminal fragments of PTH (C-PTH) appear to have biological properties different from those mediated by the amino-terminal portions of PTH and PTH-related peptide (PTHrP). To characterize a C-PTH receptor that may be involved in mediating these functions, we performed RRAs and affinity cross-linking studies with several clonal cell lines. Radiolabeled recombinant [Leu8,18,Tyr34]human PTH-(1-84)[mutPTH-(1-84) and [Tyr34] human PTH-(19-84)[mutPTH-(19-84) showed little or no specific binding to stably expressed recombinant PTH/PTHrP receptors. However, high affinity binding was observed using osteoblast-like and rat parathyroid (PT-r3) cells. The apparent Kd values were 20-30 nM for PTH-(1-84), mutPTH-(1-84), and mutPTH-(19-84), respectively; 400-800 nM for PTH-(39-84); and more than 5000 nM for PTH-(53-84). [Nle8,18,Tyr34]bovine PTH-(1-34)amide [PTH-(1-34)], PTH-(44-68), PTHrP-(37-74), and PTHrP-(109-141) showed no displacement of either radioligand. C-PTH receptor number was increased up to 2-fold by pretreating ROS 17/2.8 cells with increasing doses of PTH-(1-34), PTH-(1-84), or 8-bromo-cAMP, whereas no change was observed in response to dexamethasone or PTH-(39-84). Cross-linking studies using radiolabeled mutPTH-(1-84) or mutPTH-(19-84) revealed specific labeling of two proteins in ROS 17/2.8 cells that were approximately 40 and 90 kilodaltons in size (including the radioligand of approximately 10 kilodaltons). The intensity of affinity labeling of both proteins was dose dependently inhibited by increasing concentrations of unlabeled PTH-(1-84) and several carboxyl-terminal PTH-(1-84) fragments, but not by PTH-(1-34). Similar studies with PT-r3 cells revealed only a single protein band of about 90 kilodaltons. These data indicate that the carboxyl-terminal portion of PTH-(1-84) binds specifically to a unique receptor/binding protein distinct from the previously isolated PTH/PTHrP receptor.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Inomata N,Akiyama M,Kubota N,Jüppner Hdoi
10.1210/endo.136.11.7588200subject
Has Abstractpub_date
1995-11-01 00:00:00pages
4732-40issue
11eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Elevated activin A levels in inhibin-deficient mice promote the development of gonadal tumors and induce cachexia by reducing muscle, liver, stomach, and fat mass. Because activin A is an important regulator of tissue growth, inhibiting the actions of this TGFβ family ligand may halt or reverse pathology in diseased t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1052
更新日期:2011-10-01 00:00:00
abstract::We studied the interactions of two natural thyroid hormone receptor (TR) mutants from patients with resistance to thyroid hormone (RTH) and an artificial TR mutant with a nuclear receptor corepressor, N-CoR, and a steroid receptor coactivator, SRC-1. In electrophoretic mobility shift assays, wild-type TRbeta-1 interac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.10.6218
更新日期:1998-10-01 00:00:00
abstract::The effect of [D-Trp6]LHRH, a potent agonist of LHRH, on pituitary-testicular responses was investigated in male rhesus monkeys. Acute administration of 5, 25, or 500 micrograms [D-Trp6]LHRH produced dose-related increases in serum testosterone and bioassayable LH levels. The administration of 500 micrograms [D-Trp6]L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1308
更新日期:1982-04-01 00:00:00
abstract::Mutations in Na(+)-glucose transporters (SGLT)-2 and hepatocyte nuclear factor (HNF)-1alpha genes have been related to renal glycosuria and maturity-onset diabetes of the young 3, respectively. However, the expression of these genes have not been investigated in type 1 and type 2 diabetes. Here in kidney of diabetic r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1088
更新日期:2008-02-01 00:00:00
abstract::In the corpus luteum, prostaglandin F2 alpha (PGF2 alpha) appears to be a physiological agent with both antisteroidogenic and luteolytic actions. It is hypothesized that the antisteroidogenic action of PGF2 alpha acts through altered transport of cholesterol to the mitochondrial cytochrome P450 side-chain cleavage enz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588230
更新日期:1995-11-01 00:00:00
abstract::Recent evidence suggests that inflammation may be a common underlying cause of many obesity-associated conditions. To test whether obesity changes the response to inflammation, we investigated its effects on the acute phase of the inflammatory response to an endogenous pathogen, lipopolysaccharide (LPS). Diet-induced ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0526
更新日期:2009-11-01 00:00:00
abstract::We studied the effects of 17 beta-estradiol (E2) and PTH on alkaline phosphatase activity in SaOS-2 cells that had been passaged and grown continually in the presence (SaOS + Dex) or absence (SaOS - Dex) of 10(-8) M dexamethasone (Dex). We showed that the more differentiated SaOS + Dex cells had higher alkaline phosph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299560
更新日期:1994-02-01 00:00:00
abstract::Exposure of adipocytes to glucocorticoid hormones in vitro causes inhibition of glucose transport and metabolsim. Maximal inhibition of glucose oxidations is reduced from 42-50 to 22-25 to 5-8% in young, mature, and senescent rat adipocytes, respectively. Percent values also reflect absolute reductions since basal lev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-3-831
更新日期:1976-09-01 00:00:00
abstract::Androgens play a critical role in the development and progression of prostate cancer (PCa), and androgen deprivation therapy via surgical or medical castration is front-line therapy for patients with advanced PCa. However, intratumoral testosterone levels are elevated in metastases from patients with castration-resist...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2014-1337
更新日期:2014-11-01 00:00:00
abstract::Properties of cyclic 3',5'-nucleotide phosphodiesterase in the 100,500 X g supernatant of the bovine thyroid were investigated. The enzyme activity was measured by a radioisotopic method using an anionic-exchange resin, and it was found that the activity was stimulated by Mg2+. Sephadex G-200 gel filtration separated ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-5-1534
更新日期:1978-11-01 00:00:00
abstract::Dopaminergic inhibition of PRL release stimulated by agents that affect cytosolic Ca2+ concentrations, C-kinase activity, and cAMP levels was studied in perifused rat anterior pituitary cells cultured on cytodex beads. We used A23187 (20 microM) to increase intracellular Ca2+, the phorbol ester 12-O-tetradecanoyl-phor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-439
更新日期:1985-08-01 00:00:00
abstract::The present study was undertaken to identify the mechanisms underlying the effect of insulin-like growth factor (IGF-I) on LH receptor in rat granulosa cells. Treatment with FSH, as expected, produced a substantial increase in LH receptor messenger RNA (mRNA) level, and concurrent treatment with increasing concentrati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7112
更新日期:1999-11-01 00:00:00
abstract::Angiotensin II (AII) receptors in adrenal glomerulosa cells are coupled to adenylate cyclase inhibition. We investigated the importance of cyclase inhibition in adrenal steroidogenesis by treating adrenal glomerulosa cells with the toxin of Bordetella pertussis (20 ng/ml) for 3 and 18 h. This treatment prevented inhib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1697
更新日期:1986-10-01 00:00:00
abstract::The genetically obese Zucker rat is a widely used model of early-onset obesity. Like obese children, these obese rats are hyperinsulinemic and have low GH secretion. However, data on linear growth and insulin-like growth factor-I (IGF-I) levels in this model are scanty and contradictory. In the present study, we inves...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.7509740
更新日期:1994-03-01 00:00:00
abstract::Otsuka Long-Evans Tokushima fatty (OLETF) rats lacking cholecystokinin-A receptors are hyperphagic, obese, and diabetic. Although exercise attenuates OLETF rats' obesity, the mechanisms underlying the effects of exercise are unclear. In this study, we determined the effects of running wheel activity on patterns of bod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1441
更新日期:2005-04-01 00:00:00
abstract::Cripto-1 (TDGF1) is a multifunctional signaling factor that stimulates cellular effects, including proliferation, migration, survival, epithelial-to-mesenchymal transition, and angiogenesis, to regulate embryogenesis, tissue homeostasis, and tumorigenesis. Those cell behaviors are also associated with implantation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03039
更新日期:2018-04-01 00:00:00
abstract::To assess a potential underlying rhythm in the sensitivity of the pregnant primate myometrium to oxytocin we infused five pregnant rhesus monkeys (gestational age, 121-142 days) with oxytocin at three different periods of the 24-h day, in the order night, afternoon, and morning, via the maternal inferior vena cava. Ox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1498
更新日期:1989-09-01 00:00:00
abstract::Insulinopenic diabetes mellitus in the rat is associated with reduced circulating levels of insulin-like growth factor-I (IGF-I), resulting primarily from decreased IGF-I synthesis in liver and extrahepatic sites. Plasma GH levels in these animals are also suppressed, with loss of episodic secretion and decreased pitu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-923
更新日期:1991-02-01 00:00:00
abstract::To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LH beta mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the correspondi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-773
更新日期:1990-02-01 00:00:00
abstract::Sulfonylureas are drugs widely used in the treatment of patients with type 2 diabetes mellitus. In addition to their pancreatic effect of stimulating insulin secretion, many studies suggest that sulfonylureas also have extrapancreatic actions. We have previously reported that gliclazide, a second-generation sulfonylur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0755
更新日期:2004-02-01 00:00:00
abstract::Arginine-vasopressin (AVP) is known to be involved in maintaining glucose homeostasis, and AVP-resistance is observed in poorly controlled non-insulin-dependent diabetes mellitus subjects, resulting in a lowered plasma volume. Recently we reported that V1a vasopressin receptor-deficient (V1aR(-/-)) mice exhibited a de...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1315
更新日期:2007-05-01 00:00:00
abstract::Intravascular injections of adrenocorticotropic hormone in hypophysectomized rats were approximately 3 times more effective in stimulating increases in plasma corticosteroid concentration when given at the offset rather than the onset of the daily photoperiod (12 h:12 h light-dark cycle). Reversal of the photoperiodic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-5-1875
更新日期:1978-11-01 00:00:00
abstract::Impaired regulation of hepatic glucose production is a characteristic feature of the metabolic syndrome, a cluster of diseases that includes obesity, insulin resistance, type 2 diabetes, and cardiovascular disease. It has been proposed that sustained endoplasmic reticulum stress, which appears to occur in obesity and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1014
更新日期:2006-01-01 00:00:00
abstract::Glucocorticoids regulate hippocampal function in part by modulating gene expression through the glucocorticoid receptor (GR). GR binding is highly cell type specific, directed to accessible chromatin regions established during tissue differentiation. Distinct classes of GR binding sites are dependent on the activity o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1929
更新日期:2017-05-01 00:00:00
abstract::Groups of female ferrets born in the laboratory received sc Silastic capsules containing testosterone (T), 5 alpha-dihydrotestosterone (DHT), 17 beta-estradiol (E), or no steroid for 15 days beginning on the day of birth; an additional group of male ferrets received empty sc capsules neonatally. All ferrets were gonad...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-3-773
更新日期:1982-09-01 00:00:00
abstract::Repeated injections of recombinant human (rh) activin-A over a 2- to 3-day period are reportedly needed to stimulate the in vivo secretion of FSH. In this paper we present results showing that acute treatment with rh-activin-A caused marked and dose-dependent increases in plasma FSH, but not LH, levels in adult female...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2463
更新日期:1991-11-01 00:00:00
abstract::Using a rat model of chronic central leptin infusion in which neuropeptide Y neurons develop leptin resistance, we examined whether leptin signal transduction mechanism in the hypothalamus is altered during central leptin infusion. Adult male rats were infused chronically into the lateral cerebroventricle with leptin ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0148
更新日期:2003-09-01 00:00:00
abstract::Somatostatin was infused via the portal vein at a rate of 50 ng/min in a group of eight conscious dogs beginning 30 min before and continuing for 6 h after the ingestion of an 800-g fat-protein meal. The fasting and postprandial levels of plasma somatostatin-like immunoreactivity (SLI), insulin, glucagon, and triglyce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-6-1705
更新日期:1979-06-01 00:00:00
abstract::PTH-related peptide (PTHrP) has been shown to be the major mediator of hypercalcemia of malignancy, but may also exert effects on cell growth and differentiation. The Leydig cell tumor H-500, when implanted in Fischer rats, produces abundant PTHrP and eventually causes the death of the host animal. In the present stud...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588290
更新日期:1995-12-01 00:00:00
abstract::The effects of cilostazol, a dual inhibitor of type 3 phosphodiesterase and adenosine uptake, on ion currents were investigated in pituitary GH(3) cells and pheochromocytoma PC12 cells. In whole-cell configuration, cilostazol (10 microm) reversibly increased the amplitude of Ca(2+)-activated K(+) current [I(K(Ca))]. C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1430
更新日期:2004-03-01 00:00:00