Abstract:
:Sulfonylureas are drugs widely used in the treatment of patients with type 2 diabetes mellitus. In addition to their pancreatic effect of stimulating insulin secretion, many studies suggest that sulfonylureas also have extrapancreatic actions. We have previously reported that gliclazide, a second-generation sulfonylurea, stimulates the glucose uptake by rat hindquarter skeletal muscle directly and immediately by promoting the translocation of glucose transporter 4 to the plasma membrane. The aim of our study was to approach the gliclazide intracellular signaling pathway. For this purpose, we incubated clamped and isolated soleus muscle from rat with gliclazide. The following results were obtained: 1) gliclazide stimulates insulin receptor substrate (IRS)-1-phosphatidylinositol 3 (PI3)-kinase-associated activity, and this activity is necessary for gliclazide-stimulated glucose transport; 2) gliclazide treatment produces a gradual translocation of the diacylglycerol (DAG)-dependent isoforms protein kinase C (PKC) alpha, theta, and epsilon from cytosolic to membrane fraction that is dependent on PI3-kinase and phospholipase C (PLC)-gamma activation; and 3) PKC and PLC-gamma activation is necessary for gliclazide-stimulated glucose transport. We propose a hypothetical signaling pathway by which gliclazide could stimulate IRS-1 that would allow its association with PI3-kinase, promoting its activation. PI3-kinase products could induce PLC-gamma activation, whose hydrolytic activity could activate the DAG-dependent isoforms PKC alpha, theta, and epsilon.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Rodríguez E,Pulido N,Romero R,Arrieta F,Panadero A,Rovira Adoi
10.1210/en.2003-0755subject
Has Abstractpub_date
2004-02-01 00:00:00pages
679-85issue
2eissn
0013-7227issn
1945-7170pii
en.2003-0755journal_volume
145pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Leptin regulates food intake and other activities through its hypothalamic receptor. Leptin receptors are also found in other organs, including the ovary. Direct effects of leptin in ovarian steroid production were studied in primary rat granulosa cells and in rat and human granulosa cell lines. Leptin (0.6-18 nM) sup...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6614
更新日期:1999-04-01 00:00:00
abstract::Prolactin (Prl) secreting cells in a mixed pituitary cell culture form microscopically-identifiable plaques (zones of hemolysis around the lactotropes) when incubated in a monolayer with staphylococcal protein-A-coated ovine erythrocytes in the presence of Prl antiserum and complement. Plaques form first at 15-30 min ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-1135
更新日期:1983-03-01 00:00:00
abstract::The localization of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the adrenal gland of the frog, Rana ridibunda, was examined by the indirect immunofluorescence technique. Using an antiserum directed against rat alpha-CGRP, the presence of a network of positive fibers was observed in the adrenal pare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013380
更新日期:1994-07-01 00:00:00
abstract::To examine the role of androgen receptor (AR) in Sertoli cells (SC), we used a SC-specific AR knockout (S-AR-/y) mouse to further evaluate the chronological changes of seminiferous tubules and the molecular mechanisms of SC androgen/AR signals on spermatogenesis. Testes morphology began changing as early as postnatal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0138
更新日期:2006-12-01 00:00:00
abstract::The effects of endothelin-1 (ET-1), a potent vasoconstrictor and mitogen to various cell types, on proliferation and differentiated functions of the murine Leydig tumor cell line MA-10 were investigated. Kinetic binding experiments at room temperature showed that [125I] ET-1 bound to MA-10 cells and reached equilibriu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425480
更新日期:1993-02-01 00:00:00
abstract::The enzyme nitric oxide (NO) synthase is present in the paraventricular nucleus, while nitric oxide has recently been shown to inhibit the stimulated release of corticotrophin-releasing hormone (CRH) in vitro. Thus the possible role of NO in regulating, vasopressin (AVP), which also plays an important role in pituitar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.7689960
更新日期:1993-09-01 00:00:00
abstract::Starvation depresses thyroid gland function. In addition, the peripheral turnover of thyroxine (T4) is reduced, in part due to decreased fecal elimination of T4. The present studies were performed to determine if starvation also affects the deiodinative pathway for T4 degradation. Rats were isotopically equilibrated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1525
更新日期:1975-06-01 00:00:00
abstract::In humans, there is a significant decrease in serum T(3) and increase in rT(3) at different time points after myocardial infarction, whereas serum TSH and T(4) remain unaltered. We report here a time course study of pituitary-thyroid function and thyroid hormone metabolism in rats subjected to myocardial infarction by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0043
更新日期:2007-10-01 00:00:00
abstract::We have found notable sex difference in peptidylarginine deiminase (EC 3.5.3.15) in rat pituitaries. Pituitaries collected from 3-week to 6-month-old male rats, and those from 3-week-old females showed negligible enzyme activities. The enzyme activity of female pituitaries increased markedly by 2 months, and even furt...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2666
更新日期:1989-06-01 00:00:00
abstract::Progesterone (P4) inhibits apoptosis of rat granulosa cells and spontaneously immortalized granulosa cells (SIGCs), which were derived from rat granulosa cells. Defining the mechanism through which P4 mediates its action has been difficult because these cells do not express the classic nuclear P4 receptor. Previous st...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0067
更新日期:2004-06-01 00:00:00
abstract::The differential regulation of neurotensin (NT), calcitonin (CT), and CT gene-related peptide (CGRP) production was studied in the clonal, rat C cell-derived, 44-2C cell line. Two experimental paradigms were used: cells were incubated with maximally effective concentrations of calcium (4.0 mM); alternatively, cells we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-1-361
更新日期:1987-07-01 00:00:00
abstract::Chloroquine is widely used as a lysosomal enzyme inhibitor, and while effects on DNA repair and membrane recycling have also been reported, there has been no investigation of effects on the processing of secretory products. To determine whether chloroquine had any effect on secretion and secretory organelles, we monit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-3-725
更新日期:1984-03-01 00:00:00
abstract::Activators of peroxisome proliferator activated receptor (PPAR) regulate fatty acid metabolism and can induce adipocyte differentiation. We show here that the gamma subtype of PPAR is expressed at high levels in adipose tissue in contrast to a variety of other tissues, where little gene expression was noted. In additi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.2.8033830
更新日期:1994-08-01 00:00:00
abstract::Germ cells were isolated from 30-day-old rat testes and purified by a Percoll gradient. Germ cells were cultured for 24 h to eliminate contaminant cells by utilizing the property of many nongerm cells of attaching to plastic surfaces. Germ cells were subsequently seeded on top of 7-day-old Sertoli cell monolayers. RNA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1796
更新日期:1985-11-01 00:00:00
abstract::Morphoregulator genes like members of the Hox gene family regulate uterine development and are associated with endocrine-related processes such as endometrial proliferation and differentiation in the adult uterus. Exposure to neonatal endocrine disruptors could affect signaling events governed by Hox genes, altering t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0651
更新日期:2008-11-01 00:00:00
abstract::Sertoli cell intracellular protein 1 (SCc1) and 2 (SCc2) are polypeptides found in rat Sertoli cell cultures incubated with either FSH or (Bu)2cAMP. They were first identified in [35S]methionine-labeled Sertoli cell lysates using two-dimensional gel electrophoresis. Here we extend these observations by showing that SC...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1330490
更新日期:1992-11-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::A model for the study of testicular function in experimental uremia induced by subtotal nephrectomy in mature male rats is described. Glomerular filtration rate was reduced by 87%, resulting in stable uremia for up to 13 weeks with characteristic biochemical changes and reduction in weight of testes, prostate, seminal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1974
更新日期:1985-11-01 00:00:00
abstract::A proteinaceous secretion from phagocytizing polymorphonuclear leukocytes, termed "leukocytic endogenous mediator" (LEM), has been shown to have marked effects on hepatic amino acid transport and RNA and protein synthesis. A single injection of LEM results in a marked accumulation of labeled nonmetabolizable model ami...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-3-651
更新日期:1975-03-01 00:00:00
abstract::The FOXO family of forkhead transcription factors stimulates the transcription of target genes involved in many fundamental cell processes, including cell survival, cell cycle progression, DNA repair, and insulin sensitivity. The activity of FOXO proteins is principally regulated by activation of protein kinase B (PKB...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0481
更新日期:2003-12-01 00:00:00
abstract::Leptin has been shown to activate multiple signaling molecules in cultured cells, including Janus kinase-2, STAT (signal transducer and activator of transcription) proteins, and mitogen-activated protein kinase, and to stimulate the DNA-binding activity of STAT3 in mouse hypothalamus. In this study, the activation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.11.6301
更新日期:1998-11-01 00:00:00
abstract::The circadian rhythm of blood corticosterone was examined in rats whose feeding was restricted to a specific time of day. The peak of the corticosterone level was found just before feeding time regardless of whether, the rats were kept on a 12-h light, 12-h dark cycle or in constant light or were blinded. It took at l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-3-918
更新日期:1980-03-01 00:00:00
abstract::The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.7689950
更新日期:1993-09-01 00:00:00
abstract::Studies in rodents demonstrate that the mitogen, IGF-I, stimulates intestinal peptide YY (PYY) expression. To investigate whether the stimulatory influence of IGF-I is exerted at the level of gene transcription, rat PYY 5'-upstream sequences (-2800/+37 bp, -770/+37 bp, -127/+37 bp) fused to the firefly luciferase (luc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0770
更新日期:2004-02-01 00:00:00
abstract::To evaluate the possible existence of masked gonadotropin receptors in the corpus luteum, we characterized the effects of alcohols and neuraminidase on [125I]iodohuman LH binding to in vitro preparations of luteal tissue from the rhesus monkey and pseudopregnant rat. The presence of 1-8% (vol/vol) ethanol enhanced spe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-747
更新日期:1985-08-01 00:00:00
abstract::A [3H]colchicine-binding assay was employed for estimating the relative amounts of soluble and polymerized tubulin present in an individual anterior pituitary lobe. Colchicine binding to these two tubulin pools was time dependent and dose responsive. Scatchard analysis of the binding revealed that [3H]colchicine bound...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-1-114
更新日期:1988-01-01 00:00:00
abstract::Synthetic glucocorticoids (GCs), including dexamethasone (DEX), are powerful anti-inflammatory drugs. Long-term use of GCs, however, can result in metabolic side effects such as hyperglycemia, hepatosteatosis, and insulin resistance. The GC receptor (GR) and liver X receptors (LXRα and LXRβ) regulate overlapping genes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00094
更新日期:2017-04-01 00:00:00
abstract::Interaction between Sertoli cells and germ cells is likely to be critical for normal development of the testis. We have established and characterized cocultures of neonatal Sertoli cells and gonocytes and have begun to study the physical and functional relationship between these cells in vitro. Cells were isolated fro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-2812
更新日期:1990-12-01 00:00:00
abstract::The influences of mating on serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels were evaluated in 4-day cyclic proestrous rats of the Wistar strain. Blood samples were collected via guillotine from mated and unmated controls at 1900-2000 h or 2100--2200 h. Serum LH levels were determined by tw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-394
更新日期:1977-02-01 00:00:00
abstract::Recent studies suggest that hyperinsulinemia and insulin resistance are linked to Alzheimer's disease (AD). In this study, we used Tg2576 transgenic (Tg) mice, a widely used transgenic mouse model for AD, to explore the relationship between increased amyloid beta-peptide (Abeta) and insulin resistance. When fed a high...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1196
更新日期:2010-04-01 00:00:00