Abstract:
:Prolactin (Prl) secreting cells in a mixed pituitary cell culture form microscopically-identifiable plaques (zones of hemolysis around the lactotropes) when incubated in a monolayer with staphylococcal protein-A-coated ovine erythrocytes in the presence of Prl antiserum and complement. Plaques form first at 15-30 min and are maximal in size and number at 2 h. Approximately 70% of the adenohypophyseal cells form plaques under these conditions. TRH increases, and dopamine decreases, the size and number of plaques at early times during incubation. This reverse hemolytic plaque assay probably can be used to detect any cell secretion for which an antibody is available. This technique, or a modified version of it in which sequential plaque assays are performed on identified cells--used together with immunocytochemistry, autoradiography or electron microscopy of those cells--should provide better answers to commonly asked questions about secretory systems: Do all or only a subset of cells containing the same hormone respond to a particular secretagogue? Can cells that contain two hormones release one of them preferentially?
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Neill JD,Frawley LSdoi
10.1210/endo-112-3-1135subject
Has Abstractpub_date
1983-03-01 00:00:00pages
1135-7issue
3eissn
0013-7227issn
1945-7170journal_volume
112pub_type
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