Glucose-oxidized low-density lipoproteins enhance insulin-like growth factor I-stimulated smooth muscle cell proliferation by inhibiting integrin-associated protein cleavage.


:Prior published reports have demonstrated that glucose-oxidized low-density lipoproteins (g-OxLDL) enhance the proliferative response of vascular smooth muscle cells (SMC) to IGF-I. Our previous studies have determined that the regulation of cleavage of integrin-associated protein (IAP) by matrix-metalloprotease-2 (MMP-2) in diabetic mice in response to hyperglycemia is a key regulator of the response of SMC to IGF-I. Because chronic hyperglycemia enhances glucose-induced LDL oxidation, these studies were conducted to determine whether g-OxLDL modulates the response of SMC to IGF-I by regulating MMP-2-mediated cleavage of IAP. We determined that exposure of SMC to g-OxLDL, but not native LDL, was sufficient to facilitate an increase in cell proliferation in response to IGF-I. Exposure to an anti-CD36 antibody, which has been shown to inhibit g-OxLDL-mediated signaling, inhibited the effects of g-OxLDL on IGF-I-stimulated SMC proliferation. The effect of g-OxLDL could be attributed, in part, to an associated decrease in proteolytic cleavage of IAP leading to increase in the basal association between IAP and Src homology 2 domain-containing protein tyrosine phosphatase substrate-1, which is required for IGF-I-stimulated proliferation. The inhibitory effect of g-OxLDL on IAP cleavage appeared to be due to its ability to decrease the amount of activated MMP-2, the protease responsible for IAP cleavage. In conclusion, these data provide a molecular mechanism to explain previous studies that have reported an enhancing effect of g-OxLDL on IGF-I-stimulated SMC proliferation.






Allen LB,Capps BE,Miller EC,Clemmons DR,Maile LA




Has Abstract


2009-03-01 00:00:00














  • A novel pathway of prostacyclin signaling-hanging out with nuclear receptors.

    abstract::Prostacylin (PGI(2)), one of the major prostaglandins, is derived from arachidonic acid by the action of the cyclooxygenase (COX) system coupled to PGI(2) synthase (PGIS). The presence of the COX-2/PGIS at the nuclear and endoplasmic reticular membrane suggests differential signaling pathways of PGI(2) actions involvi...


    pub_type: 杂志文章,评审


    authors: Lim H,Dey SK

    更新日期:2002-09-01 00:00:00

  • Caffeine inhibits EGF-stimulated trophoblast cell motility through the inhibition of mTORC2 and Akt.

    abstract::Impaired trophoblast invasion is associated with pregnancy disorders such as early pregnancy loss and preeclampsia. There is evidence to suggest that the consumption of caffeine during pregnancy may increase the risk of pregnancy loss; however, little is known about the direct effect of caffeine on normal trophoblast ...


    pub_type: 杂志文章


    authors: Grant I,Cartwright JE,Lumicisi B,Wallace AE,Whitley GS

    更新日期:2012-09-01 00:00:00

  • Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide.

    abstract::To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP-B r...


    pub_type: 杂志文章


    authors: Suga S,Nakao K,Hosoda K,Mukoyama M,Ogawa Y,Shirakami G,Arai H,Saito Y,Kambayashi Y,Inouye K

    更新日期:1992-01-01 00:00:00

  • Endotoxin injection increases growth hormone and somatostatin secretion in sheep.

    abstract::Endotoxin has been shown to stimulate GH secretion in human and sheep. However, changes in hypothalamic neurohormones involved in the GH regulation by endotoxin have never been studied in vivo. In sheep it is possible to collect hypophysial portal blood (HPB) and quantify GH-releasing hormone (GHRH) and somatostatin (...


    pub_type: 杂志文章


    authors: Briard N,Guillaume V,Frachebois C,Rico-Gomez M,Sauze N,Oliver C,Dutour A

    更新日期:1998-06-01 00:00:00

  • Changes in hypophyseal hormones associated with accelerated aging and tumorigenesis of the ovaries in neonatally thymectomized mice.

    abstract::Neonatal thymectomy at 3 days of age in mice results in ovarian dysgenesis, characterized by early follicular and oocyte loss, followed by a high incidence of ovarian tumorigenesis. Thymectomized animals with dysgenetic ovaries had extremely high levels of circulating PRL and gonadotropins before the appearance of the...


    pub_type: 杂志文章


    authors: Michael SD,Taguchi O,Nishizuka Y

    更新日期:1981-06-01 00:00:00

  • Raloxifene enhances material-level mechanical properties of femoral cortical and trabecular bone.

    abstract::We have previously documented that raloxifene enhances the mechanical properties of dog vertebrae independent of changes in bone mass, suggesting a positive effect of raloxifene on material-level mechanical properties. The goal of this study was to determine the separate effects of raloxifene on the material-level mec...


    pub_type: 杂志文章


    authors: Allen MR,Hogan HA,Hobbs WA,Koivuniemi AS,Koivuniemi MC,Burr DB

    更新日期:2007-08-01 00:00:00

  • In vivo generation of hydrogen peroxide in the rat corpus luteum during luteolysis.

    abstract::The hypothesis that hydrogen peroxide generation occurs in the corpora lutea of superovulated rats during luteolysis was tested using a peroxide-dependent inhibitor of catalase, 3-amino-1,2,4-triazole (AT). Luteal regression was induced during midpseudopregnancy by injection of 500 micrograms prostaglandin F2 alpha (P...


    pub_type: 杂志文章


    authors: Riley JC,Behrman HR

    更新日期:1991-04-01 00:00:00

  • Crucial role of activin a in tubulogenesis of endothelial cells induced by vascular endothelial growth factor.

    abstract::The present study was conducted to elucidate the role of activin A in tubulogenesis of vascular endothelial cells. Activin A was produced in bovine aortic endothelial cells (BAEC). These cells also expressed the type I and type II activin receptors. When added to BAEC cultured in a collagen gel, activin A induced capi...


    pub_type: 杂志文章


    authors: Maeshima K,Maeshima A,Hayashi Y,Kishi S,Kojima I

    更新日期:2004-08-01 00:00:00

  • Differential regulation of insulin-like growth factor-I (IGF-I) and IGF-II release from cultured neonatal mouse calvaria by parathyroid hormone, transforming growth factor-beta, and 1,25-dihydroxyvitamin D3.

    abstract::In a previous study we found that PTH stimulated bone resorption and release of insulin-like growth factor-I (IGF-I) and IGF-II from cultured neonatal mouse calvaria. Since IGF-I and IGF-II stimulate osteoblast proliferation and collagen synthesis, these results suggested that increased release of IGFs during resorpti...


    pub_type: 杂志文章


    authors: Linkhart TA,Keffer MJ

    更新日期:1991-03-01 00:00:00

  • Arginine vasopressin triggers intracellular calcium release, a calcium-activated potassium current and exocytosis in identified rat corticotropes.

    abstract::Arginine vasopressin (AVP) stimulates the secretion of ACTH from pituitary corticotropes. We investigated the action of AVP in single corticotropes of male rats. Corticotropes were identified with the reverse hemolytic plaque assay using antibodies against ACTH. Using the whole-cell recording technique in conjunction ...


    pub_type: 杂志文章


    authors: Tse A,Lee AK

    更新日期:1998-05-01 00:00:00

  • Nuclear 3,5,3'-triiodothyronine (T3) in brown adipose tissue: receptor occupancy and sources of T3 as determined by in vivo techniques.

    abstract::The amount and sources of T3 associated with high affinity, low capacity cellular nuclear receptors in brown adipose tissue (BAT) have been estimated by in vivo pulse-labeling techniques. Maximal binding capacity was measured by in vivo saturation analysis. Nuclear receptor occupancy at endogenous levels of T3 and T4 ...


    pub_type: 杂志文章


    authors: Bianco AC,Silva JE

    更新日期:1987-01-01 00:00:00

  • Minireview: Cyclin D1: normal and abnormal functions.

    abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyro...


    pub_type: 杂志文章,评审


    authors: Fu M,Wang C,Li Z,Sakamaki T,Pestell RG

    更新日期:2004-12-01 00:00:00

  • Variation in myometrial response to intravenous oxytocin administration at different times of the day in the pregnant rhesus monkey.

    abstract::To assess a potential underlying rhythm in the sensitivity of the pregnant primate myometrium to oxytocin we infused five pregnant rhesus monkeys (gestational age, 121-142 days) with oxytocin at three different periods of the 24-h day, in the order night, afternoon, and morning, via the maternal inferior vena cava. Ox...


    pub_type: 杂志文章


    authors: Honnebier MB,Myers T,Figueroa JP,Nathanielsz PW

    更新日期:1989-09-01 00:00:00

  • Angiotensin II-induced mitogen-activated protein kinase phosphatase-1 expression in bovine adrenal glomerulosa cells: implications in mineralocorticoid biosynthesis.

    abstract::Angiotensin II (AngII) stimulates aldosterone biosynthesis in the zona glomerulosa of the adrenal cortex. AngII also triggers the MAPK pathways (ERK1/2 and p38). Because ERK1/2 phosphorylation is a transient process, phosphatases could play a crucial role in the acute steroidogenic response. Here we show that the dual...


    pub_type: 杂志文章


    authors: Casal AJ,Ryser S,Capponi AM,Wang-Buholzer CF

    更新日期:2007-11-01 00:00:00

  • Identification and characterization of binding proteins for inhibin and activin in human serum and follicular fluids.

    abstract::Inhibins and activins are produced by a variety of tissues and may have important endocrine and paracrine roles in development, reproduction, and hematopoiesis. However, little is known regarding the physical properties or concentrations of inhibin and activin in biological fluids. Binding proteins for inhibin or acti...


    pub_type: 杂志文章


    authors: Krummen LA,Woodruff TK,DeGuzman G,Cox ET,Baly DL,Mann E,Garg S,Wong WL,Cossum P,Mather JP

    更新日期:1993-01-01 00:00:00

  • Fetal programming: prenatal testosterone treatment causes intrauterine growth retardation, reduces ovarian reserve and increases ovarian follicular recruitment.

    abstract::Exposure to testosterone (T) during d 30-90 of fetal life results in low-birth-weight offspring, hypergonadotropism, multifollicular ovaries, and early cessation of cyclicity. The multifollicular phenotype may result from failure of follicles to regress and consequent follicular persistence or, alternatively, increase...


    pub_type: 杂志文章


    authors: Steckler T,Wang J,Bartol FF,Roy SK,Padmanabhan V

    更新日期:2005-07-01 00:00:00

  • Protease-resistant insulin-like growth factor (IGF)-binding protein-4 inhibits IGF-I actions and neointimal expansion in a porcine model of neointimal hyperplasia.

    abstract::IGF-I has been shown to play a role in the progression of atherosclerosis in experimental animal models. IGF-binding protein-4 (IGFBP-4) binds to IGF-I and prevents its association with receptors. Overexpression of a protease-resistant form of IGFBP-4 has been shown to inhibit the ability of IGF-I to stimulate normal ...


    pub_type: 杂志文章


    authors: Nichols TC,Busby WH Jr,Merricks E,Sipos J,Rowland M,Sitko K,Clemmons DR

    更新日期:2007-10-01 00:00:00

  • Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behavior.

    abstract::The crystal structure of human CG reveals that each subunit is a member of the superfamily of cystine-knot growth factors. Although the distribution of the cysteine residues in all the beta-subunits is conserved, the conformation of the human FSH dimer differs from that of the CG/LH dimers. This suggests that the func...


    pub_type: 杂志文章


    authors: Hiro'oka T,Maassen D,Berger P,Boime I

    更新日期:2000-12-01 00:00:00

  • Isolation and characterization of fragments of reduced and S-carbamidomethylated human growth hormone produced by plasmin digestion. I. Chemistry.

    abstract::Reduced and S-carbamidomethylated human GH (RCAM-hGH) was digested with human plasmin, yielding a mixture of products. These were partially separated by chromatography on DEAE-cellulose, yielding three major fractions: Da and Db, which were equipotent with native hGH in the weight gain test; and Dc, which was about ha...


    pub_type: 杂志文章


    authors: Mills JB,Kostyo JL,Moseley MH,Reagan CR,Wilhelmi AE

    更新日期:1978-05-01 00:00:00

  • The aryl hydrocarbon receptor pathway and sexual differentiation of neuroendocrine functions.

    abstract::Historically, much of the research on health effects of environmental pollutants focused on ascertaining whether compounds were carcinogenic. More recent findings show that environmental contaminants also exert insidious effects by disrupting hormone action. Of particular concern are findings that developmental exposu...


    pub_type: 杂志文章,评审


    authors: Petersen SL,Krishnan S,Hudgens ED

    更新日期:2006-06-01 00:00:00

  • Effect of ACTH and histamine stress on serum corticosterone and adrenal cyclic AMP levels in immature rats.

    abstract::Adrenal cAMP and plasma corticosterone levels were determined in pre-weanling rats subjected to treatment with either ACTH (50 mU/rat) or histamine dihydrochloride (0.2 mg/g body wt). ACTH injection elevated both serum corticosterone and adrenal cAMP levels on all days tested. However, the ACTH-induced elevation of ad...


    pub_type: 杂志文章


    authors: Cote TE,Yasumura S

    更新日期:1975-04-01 00:00:00

  • Graves' hyperthyroidism and the hygiene hypothesis in a mouse model.

    abstract::Graves' hyperthyroidism is an organ-specific autoimmune disease mediated by stimulatory autoantibodies against the TSH receptor (TSHR; thyroid-stimulating antibodies), causing thyroid hyperplasia and hyperthyroidism. Development of this ailment is well known to be under polygenic and environmental control. For example...


    pub_type: 杂志文章


    authors: Nagayama Y,McLachlan SM,Rapoport B,Oishi K

    更新日期:2004-11-01 00:00:00

  • Somatostatin is a regulator of thyrotropin secretion in the perinatal rat.

    abstract::The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set...


    pub_type: 杂志文章


    authors: Theodoropoulos TJ

    更新日期:1985-10-01 00:00:00

  • Effects of transforming growth factors and inhibin-related proteins on rat preovulatory graafian follicles in vitro.

    abstract::In view of recent reports on ovarian production and action of transforming growth factors (TGFs) and inhibin-related proteins (inhibin, activin, and follistatin), we have examined the effects of these hormones on the function of preovulatory follicles in vitro. Individual preovulatory follicles were obtained from PMSG...


    pub_type: 杂志文章


    authors: Tsafriri A,Vale W,Hsueh AJ

    更新日期:1989-10-01 00:00:00

  • Platelet-derived growth factor effects on purified testicular peritubular myoid cells: binding, cytosolic Ca2+ increase, mitogenic activity, and extracellular matrix production enhancement.

    abstract::The response of purified rat testicular peritubular myoid cells (PMC) to platelet-derived growth factor (PDGF) was studied. Freshly isolated PMC were devoid of measurable amounts of PDGF-binding sites. However, after 1 day in culture in serum-free conditions, specific high affinity receptors were detected. The estimat...


    pub_type: 杂志文章


    authors: Gnessi L,Emidi A,Scarpa S,Palleschi S,Ragano-Caracciolo M,Silvestroni L,Modesti A,Spera G

    更新日期:1993-10-01 00:00:00

  • The low-density lipoprotein receptor is regulated by estrogen and forms a functional complex with the estrogen-regulated protein ezrin in pituitary GH3 somatolactotropes.

    abstract::Estrogen regulates the function, growth, and proliferation of lactotropes in the pituitary. We report here that low-density lipoprotein (LDL) receptor (LDLR) gene expression and LDL uptake are strongly up-regulated by estrogen in pituitary somatolactotropic GH(3) cells. The uptake of LDL was significantly inhibited by...


    pub_type: 杂志文章


    authors: Smith PM,Cowan A,White BA

    更新日期:2004-07-01 00:00:00

  • A novel inhibitory protein in adipose tissue, the aldo-keto reductase AKR1B7: its role in adipogenesis.

    abstract::The aldo-keto reductase 1B7 (AKR1B7) encodes an aldose-reductase that has been reported as a detoxification enzyme until now. We have demonstrated that AKR1B7 is differently expressed in various mouse white adipose tissues depending on their location. Its expression is associated with a higher ratio of preadipocytes v...


    pub_type: 杂志文章


    authors: Tirard J,Gout J,Lefrançois-Martinez AM,Martinez A,Begeot M,Naville D

    更新日期:2007-05-01 00:00:00

  • Early short photoperiod effects in female LSH/SsLak Syrian hamsters.

    abstract::Previous experiments showed that LSH/SsLak female hamsters cease ovarian cyclicity after 2-4 weeks of short photoperiod (SP) treatment. This study was designed to reveal early hormonal and histological changes on day 1 of the estrous cycle (estrus) in SP-treated animals and changes brought about by the SP-induced acyc...


    pub_type: 杂志文章


    authors: Hauser UE,Benson B

    更新日期:1987-01-01 00:00:00

  • Hypothalamic agouti-related protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting.

    abstract::Agouti-related protein (AGRP) is a homologue of the agouti gene product and, when overexpressed, promotes obesity. Like neuropeptide Y (NPY) messenger RNA (mRNA), AGRP mRNA is produced in the hypothalamic arcuate nucleus and is elevated in leptin-deficient ob/ob and leptin-resistant db/db mice. These data suggest that...


    pub_type: 杂志文章


    authors: Mizuno TM,Mobbs CV

    更新日期:1999-02-01 00:00:00

  • Transcript expression of the tuberoinfundibular peptide (TIP)39/PTH2 receptor system and non-PTH1 receptor-mediated tonic effects of TIP39 and other PTH2 receptor ligands in renal vessels.

    abstract::Although lower than in brain, the type 2 PTH receptor (PTH2-R) has been shown to be expressed throughout the cardiovascular system. Tuberoinfundibular peptide (TIP) purified from brain is thought to be the endogenous selective ligand of the PTH2-R. In the present studies, TIP and PTH2-R mRNA expressions were evidenced...


    pub_type: 杂志文章


    authors: Eichinger A,Fiaschi-Taesch N,Massfelder T,Fritsch S,Barthelmebs M,Helwig JJ

    更新日期:2002-08-01 00:00:00