Abstract:
:Exposure of adipocytes to glucocorticoid hormones in vitro causes inhibition of glucose transport and metabolsim. Maximal inhibition of glucose oxidations is reduced from 42-50 to 22-25 to 5-8% in young, mature, and senescent rat adipocytes, respectively. Percent values also reflect absolute reductions since basal levels of glucose oxidation per cell are constant at all ages. Adipocytes of CD strain rats continue to increase in size throughout their lifespan, while cell size remains constant during the latter 80% of the Wistar adipocyte lifespan. Thus, cellular age, as well as possibly size, seems to be associated with these changes since they occur in adipocytes of both strains. Concentrations as well as absolute numbers of presumptive glucocorticoid receptors per cell are progressively reduced during maturation and aging of adipocytes in both rat strains. Glucocorticoid effects are known to require about 2 h and can be blocked by various antimetabolites during this period, reminiscent of classical steroid receptor-mediated responses. Thus, gradual loss of glucocorticoid receptors from adipocytes during maturation and aging may be related to progressively decreased glucocorticoid responsiveness.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Roth GS,Livingston JNdoi
10.1210/endo-99-3-831subject
Has Abstractpub_date
1976-09-01 00:00:00pages
831-9issue
3eissn
0013-7227issn
1945-7170journal_volume
99pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::To identify factors that regulate the tempo of growth and puberty, the present study examined how the environment influenced the timing of menarche and first ovulation in rhesus monkeys and how these events were related to differential rates of growth. Spring-born females were raised from 12 months of age under natura...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2653
更新日期:1988-12-01 00:00:00
abstract::The aim of this study was to characterize the dynamics and functional relevance of interleukin-1 beta (IL-1 beta)-induced nitric oxide production in isolated pancreatic islets. Thus, islets were isolated from adult rats, precultured for 3-5 days in medium RPMI-1640 plus 10% fetal calf serum, and then exposed to IL-1 b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3167
更新日期:1991-12-01 00:00:00
abstract::GH feeds back at the level of the central nervous system to alter the release of somatostatin and GRF, resulting in altered GH release. The purpose of this study was to see whether the concentration of GH, impinging directly on the somatotrophs of the adenohypophysis, would alter the responsiveness of the somatotrophs...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1511
更新日期:1988-04-01 00:00:00
abstract::There is strong evidence that kisspeptin acts to regulate GnRH secretion, but whether there is also a component of action on the gonadotropes is not clear. Using quantitative RT-PCR, we found that G protein-coupled receptor-54 mRNA is expressed in ovine pituitary cell fractions enriched for gonadotropes as well as in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1425
更新日期:2008-04-01 00:00:00
abstract::It is well established that endotoxemia disrupts reproductive capability, and several proinflammatory cytokines, especially IL-1 beta, IL-6, and TNF-alpha in the brain, have been implicated in this endocrine aberration. However, no previous study has directly compared the effects of the three major proinflammatory cyt...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0644
更新日期:2003-11-01 00:00:00
abstract::Primary astroglial cells produce insulin-like growth factor (IGF)-binding proteins (IGFBPs), which modulate the biological activity of IGFs on the developing astroglia. Alterations in the synthesis of astroglial IGFBPs by hormones and growth factors may, therefore, influence the paracrine regulatory actions of IGFs. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.7691580
更新日期:1993-10-01 00:00:00
abstract::The hypothesis that hydrogen peroxide generation occurs in the corpora lutea of superovulated rats during luteolysis was tested using a peroxide-dependent inhibitor of catalase, 3-amino-1,2,4-triazole (AT). Luteal regression was induced during midpseudopregnancy by injection of 500 micrograms prostaglandin F2 alpha (P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-4-1749
更新日期:1991-04-01 00:00:00
abstract::Low basal GH secretion and reduced GH responsiveness to different GH secretagogues, including GHRF, have been reported in aged animals and humans. Parallel to the in vivo findings, an impaired GH responsiveness to GHRF is evident in somatotropes from old rats of either sex. We report here that in anterior pituitaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1649
更新日期:1987-11-01 00:00:00
abstract::In rat uterus and prostate, 7 alpha, 17 alpha-dimethyl-19-nortestosterone (DMNT) binds to the androgen receptor specifically and with high affinity. However, this steroid does not bind to glucocorticoid receptors, since it does not displace binding of [3H]triamcinolone acetonide in calf thymus cytosol. In calf uterine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1327
更新日期:1986-04-01 00:00:00
abstract::It has previously been reported that treatment of rat luteal cells and human granulosa luteal cells with hydrogen peroxide (H2O2) results in a significant inhibition of steroid production. The mechanism of inhibition in the former was found to be at the level of cholesterol transport into the mitochondria, whereas in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243310
更新日期:1993-12-01 00:00:00
abstract::Previous studies indicate that magnesium, like calcium, stimulates the release of calcitonin (CT) from the thyroid gland. On the other hand, C-cell hyperplasia has been noted in magnesium-deficient dogs and rats. To explore further possible interrelationships between magnesium and CT, 21-day-old Sprague-Dawley male ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2232
更新日期:1985-06-01 00:00:00
abstract::Invasion of human trophoblasts is promoted through activation of wingless (Wnt) signaling, suggesting a role of the pathway in placental development and morphogenesis. However, details on the process such as involvement of canonical and/or noncanonical Wnt signaling cascades as well as their target genes are largely u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0557
更新日期:2010-01-01 00:00:00
abstract::Cannabinoids (CBs) exert untoward effects on reproduction by reducing LH secretion and suppressing gonadal function. Recent evidence suggests these effects are due primarily to hypothalamic dysfunction; however, the mechanism is obscure. Using immortalized hypothalamic GnRH neurons, we find these cells produce and sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1672
更新日期:2005-10-01 00:00:00
abstract::An estrogen (E2)-dependent 90,000-92,000 M(r) protein is synthesized and released by the sheep oviduct in a temporally and regionally specific manner during the first few days of pregnancy, where it associates with the embryo. The present study was undertaken to further define the nature of this protein and its regula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.6.7750470
更新日期:1995-06-01 00:00:00
abstract::The intermediate filament protein nestin was originally found to be expressed in neuronal progenitor cells, but recent studies have shown that other cell types, including endocrine and vascular endothelial cells, express nestin. In the present study, we examined the expression and localization of nestin in the ovaries...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0774
更新日期:2008-01-01 00:00:00
abstract::This study analyzes the role of the autonomic nervous system, the pituitary gland, ACTH, dexamethasone (DEX), and thyroid hormones in the regulation of 5'deiodinase (5'D) in the hypothalamus (HP) and adrenal gland (AG) of the rat. 5'D activity was analyzed in rats under basal conditions (22 C) and during cold exposure...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628370
更新日期:1995-08-01 00:00:00
abstract::The response of fat tissue to GH or insulin-like growth factor I (IGF-I) differs between humans with hypopituitarism and those with exogenous obesity; the effects of combined GH and IGF-I administration have not been compared in these two situations. In GH-deficient dwarf rats (who have a primary GH deficiency), the e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.7.8770900
更新日期:1996-07-01 00:00:00
abstract::Fibroblasts represent one of the in vivo sites of extrahepatic insulin-like growth factor I (IGF-I) production. In this study, cultured fibroblasts prepared from the skin of neonatal rats were used as a model to assess the role of serum in regulating IGF-I messenger RNA (mRNA) levels. IGF-I mRNA, as demonstrated by No...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-2854
更新日期:1990-12-01 00:00:00
abstract::FSH and IGF-I are both important determinants of testicular development and Sertoli cell function. The present studies were performed to determine the actions of FSH and IGF-I on PI3K/AKT protein kinase signaling in immature rat Sertoli cells. Primary cultures of rat Sertoli cells were prepared from 10-d-old rats. Aft...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8838
更新日期:2002-06-01 00:00:00
abstract::Trans-10, cis-12 conjugated linoleic acid (10,12 CLA) is a dietary fatty acid that promotes weight loss and disproportionate fat loss. Obese mice fed a high-fat, high-sucrose (HFHS) diet containing 10,12 CLA are resistant to weight gain and contain markedly reduced subcutaneous fat and adiponectin, with a concurrent l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00213
更新日期:2017-09-01 00:00:00
abstract::The median eminence (ME), which is the common termination field for adenohypophysiotropic systems, has been shown to produce nitric oxide (NO), a signaling molecule involved in neuroendocrine secretion. Using an ex vivo technique, 17beta-estradiol exposure to ME fragments, including vascular tissues, stimulated NO rel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6484
更新日期:1999-02-01 00:00:00
abstract::A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-6-2210
更新日期:1984-06-01 00:00:00
abstract::A monoclonal antibody which recognizes the [125I]human GH ([125I]hGH)-binding proteins of rabbit liver has been produced using hybridoma technology. A CB6F1/J mouse was immunized over a period of 82 days with a partially purified GH receptor (GHr) preparation. On the 83rd day, spleen cells from the mouse were fused wi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-2137
更新日期:1983-06-01 00:00:00
abstract::Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0334
更新日期:2006-09-01 00:00:00
abstract::Control of trabecular bone volume is mediated by local events. Recently, it has been shown that the cytokines interleukin-1 (IL-1), tumor necrosis factor alpha (TNF alpha), and TNF beta [also called lymphotoxin (LT)] produced by immune cells all stimulate osteoclast activity, whereas the lymphokine interferon-gamma (I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2494
更新日期:1987-06-01 00:00:00
abstract::In previous studies we found that plasma angiotensinogen levels were reduced by lesions of the hypothalamic paraventricular nuclei. To determine if the decrease was caused by decreased secretion of hormones that normally stimulate angiotensinogen secretion by the liver, we correlated the changes in plasma angiotensino...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-901
更新日期:1991-08-01 00:00:00
abstract::The amount and sources of T3 associated with high affinity, low capacity cellular nuclear receptors in brown adipose tissue (BAT) have been estimated by in vivo pulse-labeling techniques. Maximal binding capacity was measured by in vivo saturation analysis. Nuclear receptor occupancy at endogenous levels of T3 and T4 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-1-55
更新日期:1987-01-01 00:00:00
abstract::The effects of secretagogue(s) from mouse decidual tissue on the release of mouse placental lactogen-II (mPL-II) were studied. Decidual tissue was obtained from 10- and 11-day-pregnant mice. The tissue was homogenized, extracted, and the tissue extract was made 50% saturated with ammonium sulfate. Both the precipitate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-3-1257
更新日期:1991-09-01 00:00:00
abstract::Arginine vasopressin (AVP) stimulates the secretion of ACTH from pituitary corticotropes. We investigated the action of AVP in single corticotropes of male rats. Corticotropes were identified with the reverse hemolytic plaque assay using antibodies against ACTH. Using the whole-cell recording technique in conjunction ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.5999
更新日期:1998-05-01 00:00:00
abstract::We examined whether mitogen-activated protein (MAP) kinase was activated by stimulation of the cAMP pathway and whether MAP kinase activation was involved in synthesis of PRL and GH in GH(3) cells. Treatment of the cells with a cAMP analog, 8-(4-chlorophenylthio)cAMP (CPT-cAMP), activated MAP kinase and increased PRL ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8226
更新日期:2001-07-01 00:00:00