Abstract:
:A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and 7.42, and thus, it is a basic protein, in contrast to either renal or plasma renin which are acidic proteins. This brain enzyme does not react with antibodies specific for dog kidney renin. Since the brain enzyme forms angiotensin I from renin substrate at neutral pH, yet can be separated from and has isoelectric points different from renal renin, it is an isoenzyme of the kidney counterpart. The majority of the renin-like activity of dog brain is due to this isoenzyme.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Husain A,Smeby RR,Wilk D,Dzau VJ,Bumpus FMdoi
10.1210/endo-114-6-2210subject
Has Abstractpub_date
1984-06-01 00:00:00pages
2210-5issue
6eissn
0013-7227issn
1945-7170journal_volume
114pub_type
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