Abstract:
:The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set-point. To study the effect of SS in the immature model, we passively immunized pregnant rats with SS antiserum to neutralize endogenous SS. Acute iv administration of 0.3 ml SS antiserum to pregnant rats (day 20 of pregnancy) resulted in a significant serum TSH elevation in both mother and fetus 60 min after injection compared to the control values [maternal TSH, 62 +/- 6 muU/ml vs. control, 43 +/- 4 (mean +/- SE; P less than 0.05); fetal TSH, 95 +/- 8 vs. control, 66 +/- 6 (P less than 0.02)]. The binding capacity of the AS in the fetal serum far exceeded the circulating SS in the fetus. Similar results were obtained when neonates 5 and 10 days of age were injected with 0.1 ml AS, ip. Further experiments were done with the use of synthetic SS. Acute iv injection of synthetic synthetic SS (1 microgram) to pregnant rats made hypothyroid by feeding of a low iodine-propylthiouracil diet for the last week of gestation suppressed the elevated serum TSH level in both mother and fetus 15 min after the injection [maternal TSH, 102 +/- 11 vs. control, 163 +/- 22 (P less than 0.05); fetal TSH, 99 +/- 9 vs. control, 143 +/- 13 (P less than 0.02)]. Similar results were obtained when hypothyroid neonates 5 and 10 days of age were injected with 1 microgram SS, ip. These findings suggest that there is similarity in the effects of SS on TSH secretion (suppression) in mother, fetus, and neonate and dependence of the perinatal TSH on SS, in contrast to TRH. It is, then, likely that TSH secretion in the perinatal rat is under the inhibitory influence of endogenous SS.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Theodoropoulos TJdoi
10.1210/endo-117-4-1683subject
Has Abstractpub_date
1985-10-01 00:00:00pages
1683-6issue
4eissn
0013-7227issn
1945-7170journal_volume
117pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The atrophy and hypofunction of the adrenal cortex following long-term pharmacologic glucocorticoid therapy is a major health problem necessitating chronic glucocorticoid replacement that often prolongs the ultimate return of endogenous adrenocortical function. Underlying this functional recovery is anatomic regenerat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03061
更新日期:2018-02-01 00:00:00
abstract::Both Snell (Pit-1(dw) or (dwj), dw/dw) and Ames (Prophet of Pit-1(df), df/df) dwarf mice fail to produce prolactin (PRL) as well as GH due to deficient transcription factor Pit-1 activity and have reduced numbers of hypothalamic PRL-inhibiting area A12 tuberoinfundibular dopaminergic (TIDA) neurons. It has been report...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0931
更新日期:2004-12-01 00:00:00
abstract::We recently identified a 32 K mol wt insulin-like growth factor (IGF)-binding protein (BP) which is markedly increased in the serum of streptozotocin-diabetic rats and recognized by antiserum against the human amniotic fluid IGFBP (hIGFBP-1). In the present study we sought to confirm that this protein represents the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-789
更新日期:1990-08-01 00:00:00
abstract::Early life exposure to immunological challenge has programming effects on the adult hypothalamo-pituitary-adrenocortical axis stress responsivity, and stress is known to suppress GnRH pulse generator activity, especially LH pulses. We investigated the effects of neonatal exposure to endotoxin on stress-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0710
更新日期:2007-12-01 00:00:00
abstract::The two peptides pancreastatin and diazepam binding inhibitor (DBI) were recently demonstrated in pancreatic islets and were shown to inhibit insulin secretion in short term experiments. In the present study we investigated long term effects of pancreastatin and DBI on the DNA synthesis, polyamine content, and insulin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3277
更新日期:1991-06-01 00:00:00
abstract::Ghrelin is a growth hormone-releasing peptide recently discovered in the stomach of rat and human as an endogenous ligand for growth hormone-secretagogue receptor. In the present study, a full-length cDNA for mouse ghrelin has been cloned from the stomach using the oligo-capping and rapid amplification methods, and th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8433
更新日期:2001-08-01 00:00:00
abstract::Ovary-specific acidic protein (OSAP) is a novel molecule discovered from a genomic project designed to identify ovary-selective genes in mice. Whereas public databases suggest extraovarian expression of OSAP, its tissue distribution has not yet been well documented. Thus, the expression profile of mouse and human OSAP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1584
更新日期:2009-07-01 00:00:00
abstract::We have analyzed the factors responsible for the circadian variation in rat hepatic mRNA-S14. Regulation of this sequence, which is found in lipogenic tissues and encodes a protein (S14) believed to be associated with fatty acid synthesis, is an excellent model of the interaction of thyroid hormone and dietary factors...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1563
更新日期:1987-04-01 00:00:00
abstract::Functional disruption of either MC3R or MC4R results in obesity, implicating both in the control of energy homeostasis. The ligands for these receptors are derived from the prohormone proopiomelancortin (POMC), which is posttranslationally processed to produce a set of melanocortin peptides with a range of activities ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0866
更新日期:2006-12-01 00:00:00
abstract::The signals that regulate cardiac myocyte maturation in the neonatal heart are not completely understood. In our study we examined the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] on primary cultures of ventricular myocytes isolated from neonatal rat hearts. Our data show that 1,25-(OH)2D3 inhibited an increase ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.2.7835280
更新日期:1995-02-01 00:00:00
abstract::During the preovulatory and estradiol-progesterone-induced GnRH-LH surge, a subpopulation of GnRH neurons transiently expresses the transcription factor c-fos, which is a useful marker of cell activation. To further characterize this subpopulation of GnRH neurons, multiple immunohistochemical procedures were applied t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7299
更新日期:2000-02-01 00:00:00
abstract::Transcriptional regulation of metabolic genes in the liver is the key to maintaining systemic energy homeostasis during starvation. The membrane-bound transcription factor cAMP-responsive element-binding protein 3-like 3 (CREB3L3) has been reported to be activated during fasting and to regulate triglyceride metabolism...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1113
更新日期:2014-12-01 00:00:00
abstract::LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867570
更新日期:1995-03-01 00:00:00
abstract::In rats, protein hydrolysates (peptones) stimulate cholecystokinin (CCK) release both in vivo and in a model of isolated vascularly perfused duodeno-jejunum. However, the mechanisms involved in peptone-induced stimulation of CCK cells are not well understood. In particular, the possibility that peptones may directly i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.5023
更新日期:1997-03-01 00:00:00
abstract::Serum LH and FSH were measured at 60, 30, and 0 min before, at 5, 15, and 30 min during, and at 10, 45, and 90 min after bilateral electrical stimulation (ES) of various hypothalamic regions in 12 unanesthetized ovariectomized rhesus monkeys. ES of the arcuate-ventromedial nuclei (medial basal hypothalamus; MBH) induc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-314
更新日期:1977-02-01 00:00:00
abstract::A miniature thermoelectric probe was used to record continuously tissue blood flow in the anterior pituitary gland of anesthetized dogs. The output of the flow probe is linear in the range of tissue blood flow encountered in the pituitary. Probe placement required minimal tissue trauma, and zero blood flow was recorde...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-3-630
更新日期:1975-09-01 00:00:00
abstract::It is established that androgens and unidentified Sertoli cell (SC)-derived factors can influence the development of adult Leydig cells (LC) in rodents, but the mechanisms are unclear. We evaluated adult LC development and function in SC-selective androgen receptor (AR) knockout (SCARKO) and complete AR knockout (ARKO...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0300
更新日期:2005-09-01 00:00:00
abstract::A detailed understanding of the mechanism of action of estrogen on bones is still lacking. The present study was designed to examine possible modulation by 17 beta-estradiol (E2) on the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and on vitamin D receptors (VDR) in the ROS 17/2.8 osteoblast-like cell line. Cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315250
更新日期:1992-05-01 00:00:00
abstract::In virtually all studies with MA-10 cells, progesterone RIAs have been used to measure steroid synthesis. To test whether progesterone is a stable end product, we investigated the metabolism of added tritiated progesterone and pregnenolone in MA-10 cells over a period of 3 h. Steroids were then extracted, separated by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8545
更新日期:2001-12-01 00:00:00
abstract::Androgen signaling in prostate smooth muscle cells (pSMCs) is critical for the maintenance of prostate homeostasis, the alterations of which are a central aspect in the development of pathological conditions. Testosterone can act through the classic androgen receptor (AR) in the cytoplasm, eliciting genomic signaling,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00718
更新日期:2018-02-01 00:00:00
abstract::We employed a short term system of dispersed adult mouse hypothalamic cells, as previously described in the rat, to examine the roles of calcium and calmodulin in SRIF release. Incubation studies were performed 24 h after hypothalamic cell dispersion. SRIF release, as determined by RIA was stimulated in a dose-depende...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-369
更新日期:1985-07-01 00:00:00
abstract::Various hormones and growth factors act at least in part by raising the cytosolic pH of the target cell. In the present studies we examined the influence of the muscarinic cholinergic agonist carbachol on intracellular pH (pHi) in chicken granulosa cells. The pHi in granulosa cells from the two largest preovulatory fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.1.1612000
更新日期:1992-07-01 00:00:00
abstract::The hepatic extractions of gastric inhibitory polypeptide (GIP) and insulin were determined using in vitro and in vivo methods to assess the role of the liver in GIP metabolism and the possible effect of GIP on the hepatic extraction of insulin. During in vitro studies using the isolated perfused rat liver, infusion o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1011
更新日期:1984-09-01 00:00:00
abstract::The study of the biochemical mechanisms regulating the differentiation of the ovarian androgen-producing cells has been difficult because of the lack of a method for isolating the theca-interstitial cells (TIC) from the granulosa and other cell types. We report here a simple, rapid and reproducible method for obtainin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2345
更新日期:1988-05-01 00:00:00
abstract::Although it is evident that androgens increase muscle mass and strength, little is known about the critical molecular targets of androgens in skeletal muscle. In rodents, the skeletal alpha-actin gene is a tissue-specific gene expressed only in the levator ani and other skeletal muscles but not in the prostate or prep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0530
更新日期:2008-03-01 00:00:00
abstract::Depletion of hypothalamic norepinephrine (NE) and epinephrine by administration of diethyldithiocarbamate abolished the stimulatory effects of intraventricular (IVT) angiotensin II (AII) on LH release in ovariectomized rats pretreated with estradiol and progesterone. The increase in blood LH produced by IVT NE or iv L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2728
更新日期:1986-12-01 00:00:00
abstract::Previous studies showed that epidermal growth factor (EGF) and TGFalpha mimic the action of LH on the resumption of oocyte maturation. We tested whether EGF-like agents, such as amphiregulin (AR), epiregulin (ER), and betacellulin (BTC), also mediate the LH stimulation of the ovulatory response in the rat. LH induced ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0588
更新日期:2005-01-01 00:00:00
abstract::We investigated the effect of central serotonin (5-hydroxytryptamine, 5-HT) administration on hypothalamic tuberoinfundibular dopamine neurons and related changes in neuronal activity to circulating PRL levels in two physiological models: 1) pregnant rats expressing (day 8) or not expressing (days 11 and 16) PRL surge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1359960
更新日期:1992-12-01 00:00:00
abstract::We compared the somatomedin (SM) activity of perfusates of livers from normal and hypophysectomized (hypox) rats with and without the addition of growth hormone (GH) to the perfusions. Livers were perfused for a total of 3 hours, with a change of Waymouth's medium MB 752/l every 30 min, in a continously recirculating ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-3-606
更新日期:1976-03-01 00:00:00
abstract::The potential role of basic fibroblast growth factor (bFGF) as a mediator of cell-cell interactions in the growth and development of the testis was examined. Nuclease protection analysis was used to evaluate bFGF gene expression in the testis and other male reproductive tract tissues. bFGF expression was evident in se...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446630
更新日期:1992-12-01 00:00:00