Abstract:
:Ovary-specific acidic protein (OSAP) is a novel molecule discovered from a genomic project designed to identify ovary-selective genes in mice. Whereas public databases suggest extraovarian expression of OSAP, its tissue distribution has not yet been well documented. Thus, the expression profile of mouse and human OSAP was determined by quantitative real-time RT-PCR using RNAs isolated from various tissues. The results demonstrate that the human and mouse OSAP expression profiles are similar; OSAP is prominently expressed in steroidogenic tissues with the highest level of expression observed in the adrenal gland. Placenta served as an exception and possessed minimal level of OSAP mRNA. Immunohistochemical studies show that mouse OSAP localizes almost exclusively to the steroid-producing cells of the ovary, adrenal gland, and testis. Consistent with predictions made by several subcellular localization algorithms, dual labeling studies in Y-1 mouse adrenocortical cells indicate OSAP resides in the mitochondria. Because of its abundant expression in steroidogenic cells and mitochondrial localization, a role for OSAP in steroidogenesis was determined. OSAP silencing by specific small interfering RNAs significantly inhibits 8-bromoadenosine-cAMP-induced progesterone production in Y-1 cells. Reduction in OSAP levels results in mitochondrial fragmentation and a decrease in the cellular content of mitochondrial DNA, indicative of decreased mitochondrial abundance. Lastly, 8-bromoadenosine-cAMP does not regulate OSAP protein expression in Y-1 cells as is the case for other steroidogenic components known to be induced by cAMP. Collectively these results suggest that OSAP is involved in steroidogenesis, potentially through its ability to maintain mitochondrial abundance and morphology.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Matsumoto T,Minegishi K,Ishimoto H,Tanaka M,Hennebold JD,Teranishi T,Hattori Y,Furuya M,Higuchi T,Asai S,Kim SH,Miyakoshi K,Yoshimura Ydoi
10.1210/en.2008-1584subject
Has Abstractpub_date
2009-07-01 00:00:00pages
3353-9issue
7eissn
0013-7227issn
1945-7170pii
en.2008-1584journal_volume
150pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Thyroid hormones are essential for normal development and metabolism. Their synthesis requires transport of iodide into thyroid follicles. The mechanisms involving the apical efflux of iodide into the follicular lumen are poorly elucidated. The discovery of mutations in the SLC26A4 gene in patients with Pendred syndro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1548
更新日期:2012-01-01 00:00:00
abstract::Antisera to porcine relaxin were produced in rabbits injected with different fractions that had been separated by polyacrylamide gel electrophoresis (PAGE). Analyses by agar double immunodiffusion demonstrated that the different fractions of relaxin separated by PAGE have similar antigenic sites and the individual fra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-3-679
更新日期:1977-09-01 00:00:00
abstract::Osteoclast precursors have not been well characterized because there are no known markers that can detect them. We have used osteoclast-like cells formed in vitro to develop a panel of specific antibodies that react with mature osteoclasts, osteoclast precursors, and other cells in the osteoclast lineage. Monoclonal a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-630
更新日期:1989-08-01 00:00:00
abstract::PTH upregulates the expression of the receptor activator of nuclear factor κB ligand (Rankl) in cells of the osteoblastic lineage, but the precise differentiation stage of the PTH target cell responsible for RANKL-mediated stimulation of bone resorption remains undefined. We report that constitutive activation of PTH ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1046
更新日期:2014-08-01 00:00:00
abstract::Nonesterified fatty acids (NEFAs) have been recently shown in the rat to be involved in steroid hormone expression, having effects on plasma transport and intracellular activity. This study examines the influence of saturated and unsaturated NEFAs on estradiol (E2) binding to cytosol from human uterus, breast, and mel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-1
更新日期:1986-01-01 00:00:00
abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-2014
更新日期:1981-05-01 00:00:00
abstract::Disruption of the pituitary adenylate cyclase-activating polypeptide (PACAP) gene in mice has demonstrated a role for this highly conserved neuropeptide in the regulation of metabolism and temperature control. Localization of PACAP neurons within hypothalamic nuclei that regulate appetite suggest PACAP may affect feed...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0515
更新日期:2008-04-01 00:00:00
abstract::Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5275
更新日期:1997-07-01 00:00:00
abstract::Axonal degeneration often leads to the death of neuronal cell bodies. Previous studies demonstrated the substantial protective role of 17β-estradiol (E2) in several types of neuron. However, most studies examined cell body protection, and the role of 17β-E2 in axonal degeneration of retinal ganglion cells (RGC) remain...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0046
更新日期:2011-07-01 00:00:00
abstract::In previous studies we demonstrated a much greater rate of glucose cycling (glucose-->glucose-6-P-->glucose) in islets from ob/ob mice than from lean litter mates. Cycling was further augmented by dexamethasone treatment. To determine whether these findings could be accounted for by increased islet glucose-6-phosphata...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720640
更新日期:1995-05-01 00:00:00
abstract::These studies have examined the ontogeny of specific GH- and PRL-binding proteins in rabbit liver cytosol across the fetal, early neonatal, and adult periods. The precise hormonal specificity of binding of the somatotropic/lactogenic ligand, [125I]human GH, was determined by displacement with monoclonal antibodies aga...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-516
更新日期:1989-07-01 00:00:00
abstract::Mitochondrial reactive oxygen species have been implicated in both diabetic complications and the progression of the underlying diabetic state. However, it is not clear whether mitochondria of diabetic origin are intrinsically altered to generate excess reactive oxygen species independent of the surrounding diabetic m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0404
更新日期:2009-01-01 00:00:00
abstract::The purpose of this study is to establish a primary LHRH cell culture system using embryonic olfactory placode and to examine whether LHRH cells derived from olfactory placode and the migratory pathway of LHRH neurons mature in vitro. Six monkey fetuses at the ages of E34-E36 were delivered surgically and the area inc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404690
更新日期:1993-11-01 00:00:00
abstract::Diabetes is associated with increased generation of cytokines and tissue inflammation, but it is unclear how increased cytokine synthesis is causally related to the development of diabetic complications. Here, we report that exposure to high (25 mm) glucose, but not iso-osmotic concentrations of mannitol or 3-methyl g...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0512
更新日期:2007-09-01 00:00:00
abstract::A subline of an x-ray-induced transplantable rat insulinoma has been studied in vivo and in vitro. Tumors grew rapidly after sc transplantation and were rich in insulin, but contained only small amounts of glucagon and somatostatin. Despite marked basal hyperinsulinemia, iv glucose administration caused a further incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2091
更新日期:1982-12-01 00:00:00
abstract::Previously, we have demonstrated an inhibitory effect of oxytocin (OXT) on progesterone (P) and androstenedione (A) release of porcine luteal cell cultures. The present study examines whether OXT modulates P, A, or estradiol (E2) release of so-called small luteal cells (SLC) or of granulosa-derived large luteal cells ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2343
更新日期:1990-05-01 00:00:00
abstract::This study was initiated to investigate the significance of uterine cell death and proliferation during the estrous cycle and early pregnancy and their correlation with sex steroids in hamsters where blastocyst implantation occurs in only progesterone-primed uteri. The results obtained in hamsters were also compared w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1555
更新日期:2006-05-01 00:00:00
abstract::Steroid hormone synthesis is a vital function of the adrenal cortex, serves a critical role in gonadal function, and maintains pregnancy if normally executed in the placenta. The substrate for the synthesis of all steroid hormones is cholesterol, and its conversion to the first steroid, pregnenolone, by the cholestero...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0541
更新日期:2009-02-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::Mediators of lymphocyte infiltration in inflammatory thyroid disease have yet to be identified. Here we examine the ability of IL-1beta to enhance the production of chemoattractants by human thyrocytes. Primary cultures, when treated with the cytokine, release T lymphocyte chemotactic activity. The effect of IL-1beta ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0199
更新日期:2003-07-01 00:00:00
abstract::The glycoprotein FSH, a product of pituitary gonadotrope cells, regulates ovarian follicle development in females and spermatogenesis in males. FSH is a heterodimer of the common α gonadotropin subunit and the hormone-specific FSHβ subunit (a product of the Fshb gene). Using a conditional knockout approach (Cre-lox), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00100
更新日期:2018-07-01 00:00:00
abstract::Plasma chromogranin-A (CgA) concentrations correlate with severe cardiovascular diseases, whereas CgA-derived vasostatin-I and catestatin elicit cardiosuppression via an antiadrenergic/nitric oxide-cGMP mediated mechanism. Whether these phenomena are related is unknown. We here investigated whether and to what extent ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2210
更新日期:2013-09-01 00:00:00
abstract::PTH-related peptide (PTHrP) has been shown to be the major mediator of hypercalcemia of malignancy, but may also exert effects on cell growth and differentiation. The Leydig cell tumor H-500, when implanted in Fischer rats, produces abundant PTHrP and eventually causes the death of the host animal. In the present stud...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588290
更新日期:1995-12-01 00:00:00
abstract::Rat uterine and anterior pituitary microsomes each contain a population of specific estrogen-binding sites. Saturation binding of estradiol is demonstrable, with an affinity similar to that of the cytosol estrogen receptor (Ka = 1-2 X 10(10) M-1). Dissociation rate kinetic determinations, however, revealed that estrog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1341
更新日期:1985-10-01 00:00:00
abstract::The possibility that the responsiveness of plasma aldosterone concentration to angiotensin II alters with changes in sodium balance was investigated in male beagle dogs under conditions of controlled sodium and potassium intake. Angiotensin II was infused at four different rates (usually 3, 6, 12, and 24 ng/kg/min), e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-2-485
更新日期:1978-02-01 00:00:00
abstract::A serially transplantable, chemically induced pancreatic islet cell tumor was developed in Lewis rats. The original tumor was induced by the administration of streptozotocin and nicotinamide. It was subsequently maintained by ip or sc transplantation of tissue fragments into recipient animals. Tumors generally grew to...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-4-954
更新日期:1980-10-01 00:00:00
abstract::The mechanisms by which bacterial endotoxin [lipopolysaccharide (LPS)] stimulates the hypothalamo-pituitary-adrenal axis (HPAA) have not been elucidated. The present study was designed to investigate the involvement of macrophages in plasma ACTH and corticosterone responses to LPS administration in rats using selectiv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-1-330
更新日期:1991-07-01 00:00:00
abstract::To obtain large amounts of hTSH and to study the role of the N-linked oligosaccharides for its biological activity, hTSH was produced using recombinant baculovirus containing the human alpha-subunit and a hTSH beta-minigene, respectively, both under the control of the polyhedrin promoter. Expression in insect cells wa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.1.4897
更新日期:1997-01-01 00:00:00
abstract::The CRH-binding protein (CRH-BP) antagonizes the ACTH-releasing activity of the neuropeptide CRH in vitro. However, the function of CRH-BP in vivo and the molecular mechanisms that regulate CRH-BP expression are not well understood. In this study, the rat CRH-BP gene was characterized, and CRH-BP promoter sequences we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5128
更新日期:1997-05-01 00:00:00
abstract::We investigated whether genetic selection for growth influences pituitary GH secretion in two strains of rats, LL (large) and SS (small). Animals were bled every 15 min for 6 h via an indwelling atrial Silastic catheter, and GH levels were determined by RIA. LL and SS males displayed a low frequency, high amplitude ep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-1948
更新日期:1986-11-01 00:00:00