Abstract:
:The purpose of this study is to establish a primary LHRH cell culture system using embryonic olfactory placode and to examine whether LHRH cells derived from olfactory placode and the migratory pathway of LHRH neurons mature in vitro. Six monkey fetuses at the ages of E34-E36 were delivered surgically and the area including the olfactory placode (PL) and the areas that encompass the migratory pathway (MP) were dissected out. The tissues were cut into small pieces and plated on collagen- or poly-L-lysine-coated glass coverslips in medium M199. Cultures were maintained for up to 33 days and immunostained for LHRH, GnRH-associated peptide, neurofilament protein, neuron-specific enolase, and glial fibrillary acidic protein. LHRH positive cells were also positive for neurofilament proteins neuron-specific enolase, and GnRH-associated peptides, but negative for glial fibrillary acidic protein. In the first week of culture, LHRH cells remained within the explants of PL, were rounded (average dimensions: 13.0 x 11.3 microns) and stained lightly. By the second week a number of LHRH cells (15.7 x 13.6 microns) with neurites started to migrate out from PL explants, whereas some still remained in the PL. By the third week a large number of LHRH cells (19.3 x 9.4 microns) had migrated out from the PL. They were fusiform in shape with clear nuclei and extended long varicose neurites up to 500 microns in length. A few "pioneer" LHRH cells appeared to lead the migration of 100-400 LHRH cells forming 1-3 major migratory paths. In contrast, LHRH cells from MP explants migrated out sooner than those from PL explants. LHRH cells from the ventral part of the MP, which is close to the PL, migrated out by 1-2 weeks and formed several migratory paths, whereas LHRH cells from the dorsal part of the MP, which is farther from the PL, were scattered widely around explants and their neurites were extended tortuously. Cultured LHRH cells released LHRH into the media and responded to challenge with high K+. The results indicate that 1) primary LHRH neurons can be obtained from the embryonic PL and their migratory pathway, 2) these neurons migrate and mature in culture and 3) they are accessible for cellular and molecular studies.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Terasawa E,Quanbeck CD,Schulz CA,Burich AJ,Luchansky LL,Claude Pdoi
10.1210/endo.133.5.8404690subject
Has Abstractpub_date
1993-11-01 00:00:00pages
2379-90issue
5eissn
0013-7227issn
1945-7170journal_volume
133pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We previously showed that receptor-like protein tyrosine phosphatase (RPTP)-alpha inhibited insulin-increased prolactin gene transcription. Others suggested that RPTPalpha was a key intermediary between integrins and activation of Src. We present evidence that inhibition of insulin-increased prolactin gene transcripti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1386
更新日期:2005-08-01 00:00:00
abstract::To elucidate the metabolic fate and possible role of androgens and their derivatives during primate fetal development, aromatase (AROM), 5 alpha-reductase (5 alpha R), and androgen receptor (AR; cytosolic) levels were assessed in the brain, heart (HRT), lung (LNG), and skeletal muscle (MUS) of fetal rhesus monkeys. An...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-627
更新日期:1989-02-01 00:00:00
abstract::The two phases of the ovulatory menstrual cycle of the primate are characterized by divergent activities of the GnRH pulse generator. During the luteal phase, LH pulse frequency is significantly reduced below that observed during the follicular phase. In this report we investigate whether the decrease in pulse frequen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-2044
更新日期:1987-05-01 00:00:00
abstract::Circulating levels of calcitonin precursors (CTpr), including procalcitonin (ProCT), increase up to several thousand-fold in human sepsis, and immunoneutralization improves survival in two animal models of this disease. Herein, we analyzed inflammation-mediated calcitonin I gene (CALC I) expression in human adipocyte ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0854
更新日期:2003-12-01 00:00:00
abstract::C-type natriuretic peptide (CNP), found in endothelial cells, chondrocytes, and neurons, binds its cognate transmembrane receptor, natriuretic peptide receptor-B (NPR-B/GC-B), and stimulates the synthesis of the intracellular signaling molecule, cGMP. The known physiologic consequences of this binding event are vasore...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220702
更新日期:2003-01-01 00:00:00
abstract::Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. H...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1016
更新日期:2014-09-01 00:00:00
abstract::Progesterone released by cumulus cells surrounding the egg induces a Ca2+ influx into human sperm cells via the cationic channel of sperm (CatSper) Ca2+ channel and controls multiple Ca2+-dependent responses essential for fertilization. We hypothesized that chemical UV filters may mimic the physiological action of pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1473
更新日期:2016-11-01 00:00:00
abstract::Aldosterone plays a major role in regulating sodium and potassium flux in epithelial tissues such as kidney and colon. Recent evidence suggests that serum- and glucocorticoid-regulated kinase (SGK) is induced by aldosterone and acts as a key mediator of aldosterone action in epithelial tissues. Induction of SGK messen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.4.8095
更新日期:2001-04-01 00:00:00
abstract::The role of GH during the ovine estrous cycle is unknown. In this study, pituitary GH mRNA and peripheral serum GH levels were measured in ewes representing the major phases of the estrous cycle. There was a significant rise (P less than 0.05) in both GH mRNA and serum GH concentrations during the follicular phase of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1474
更新日期:1989-09-01 00:00:00
abstract::The distribution and density of selectively labeled mu-, delta-, and kappa-opioid binding sites were examined by in vitro radioautography in the hypothalamus of normal, estradiol valerate (EV)-injected, and estradiol (E2)-implanted female rats. Hypothalamic beta-endorphin concentration was also examined by RIA in thes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-2969
更新日期:1990-12-01 00:00:00
abstract::These studies have examined the ontogeny of specific GH- and PRL-binding proteins in rabbit liver cytosol across the fetal, early neonatal, and adult periods. The precise hormonal specificity of binding of the somatotropic/lactogenic ligand, [125I]human GH, was determined by displacement with monoclonal antibodies aga...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-516
更新日期:1989-07-01 00:00:00
abstract::Nucleic acid-based therapies offer great potential for treatment of a variety of diseases including cancer by modulating protein expression with DNA or small interfering RNA. However, realization of their full therapeutic potential is currently limited due to an inability to reach the target site in an active form. Id...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2009-1045
更新日期:2010-02-01 00:00:00
abstract::Glucocorticoids are potent inhibitors of prostaglandin (PG) release in vitro, but they fail to influence basal PG production in vivo. To test one possible explanation for this apparent discrepancy in the present study we investigated the effect of dexamethasone (DEX) on basal and antidiuretic hormone-stimulated renal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2856
更新日期:1986-12-01 00:00:00
abstract::Thyroid hormone accelerates energy expenditure (EE) and is critical for cold-induced thermogenesis. To define the metabolic role played by thyroid hormone in the dissipation of calories from diet, hypothyroid mice were studied for 60 d in a comprehensive lab animal monitoring system. Hypothyroidism decreased caloric i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1066
更新日期:2011-09-01 00:00:00
abstract::31P nuclear magnetic resonance (NMR) was used to study the effects of 17 beta-estradiol on the content of phosphates and on the flux catalyzed by creatine kinase in immature rat uteri. Perifusion with oxygenated medium at 36 C maintained the uteri in a viable state for at least 10 h in vitro during 31P NMR measurement...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1631
更新日期:1988-04-01 00:00:00
abstract::Fibrosis (extracellular matrix accumulation) is the final end point in diabetic cardiomyopathy. The current study evaluated the therapeutic effects of the antifibrotic hormone relaxin (RLX) in streptozotocin-treated transgenic mRen-2 rats, which undergo pathological and functional features similar to human diabetes. T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0250
更新日期:2008-07-01 00:00:00
abstract::The Rice H500 rat Leydig cell tumor is a well characterized model of humoral hypercalcemia of malignancy (HHM). Circulating concentrations of PTH-related protein (PTHRP) have not been reported in this or any other animal model of HHM. Taking advantage of the marked N-terminal amino acid homology between rodent and hum...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1444
更新日期:1990-09-01 00:00:00
abstract::Daily administration of an inhibitor of 5 alpha-reductase, 17 beta-testosterone carboxylic acid, significantly attenuated the stimulatory effect of testosterone propionate on penile erections displayed by castrated male rats which were restrained in a supine position. This treatment, however, failed to affect mating b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-4-1047
更新日期:1981-10-01 00:00:00
abstract::Kisspeptin neurons play an essential role in the regulation of fertility through direct regulation of the GnRH neurons. However, the relative contributions of the two functionally distinct kisspeptin neuron subpopulations to this critical regulation are not fully understood. Here we analyzed the specific projection pa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1131
更新日期:2015-07-01 00:00:00
abstract::The effects of FSH, hCG, and PRL on the activity of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH) of separate ovarian components of hypophysectomized, diethylstilbestrol-treated rats were studied. Enzyme activity was found to reside mainly in granulosa cells. FSH induced an increase in enzyme activity. A prepar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-3-711
更新日期:1979-03-01 00:00:00
abstract::We investigated the contribution of corticotropin-releasing factor (CRF) receptors types 1 and 2 (CRF(1) and CRF(2)) in mediating the ACTH response to shock, alcohol injection, or endotoxemia in the rat. Peptidic (Astressin B and Astressin(2)-B) and nonpeptidic (NBI 30775) CRF antagonists were injected iv before the s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0117
更新日期:2003-06-01 00:00:00
abstract::The GH/IGF-I axis has both pre- and postpubertal metabolic effects. However, the differential effects of GH and/or IGF-I on animal physiology or the plasma proteome are still being unraveled. In this report, we analyzed several physiological effects along with the plasma proteome after treatment of mice with recombina...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1217
更新日期:2011-10-01 00:00:00
abstract::P19 EPI-7, a differentiated murine embryonal carcinoma cell line with an epithelioid morphology, does not require external growth factors for proliferation under clonal and subconfluent conditions. At saturation density, however, cells become quiescent in the G1/G0 phase of the cell cycle from which they can be restim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-2029
更新日期:1989-04-01 00:00:00
abstract::Oxytocin (OT) is a hypothalamic nonapeptide that is synthesized as part of a larger precursor protein that also contains an approximately 10-kDa protein called neurophysin at its C-terminus. This precursor protein is trafficked through the regulated secretory pathway into secretory granules and then axonally transport...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8700
更新日期:2002-03-01 00:00:00
abstract::A highly conserved region in the PTH promoter was identified using the basic local alignment search tool (BLAST) 2 Sequences comparison. Strong specific complexes were observed with a DNA probe that contained much of the computer-derived conserved sequence in the EMSA using bovine parathyroid gland (bPTG) nuclear extr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0119
更新日期:2003-07-01 00:00:00
abstract::Cross talks between the vascular and immune system play a critical role in vascular diseases, in particular in atherosclerosis. The osteoclast-associated receptor (OSCAR) is a regulator of osteoclast differentiation and dendritic cell maturation. Whether OSCAR plays a role in vascular biology and has an impact on athe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1282
更新日期:2011-12-01 00:00:00
abstract::Defining the molecular genetics of diabetes gives new insight into the underlying etiology and so should help improve treatment. The genetic etiology is now known for most patients with beta-cell monogenic diabetes, allowing genetic classification. We review how this genetic knowledge alters treatment. Patients with a...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0152
更新日期:2006-06-01 00:00:00
abstract::The expression of the microsomal (M) antigen on the surface and in the cytoplasm of a strain of rat thyroid cells (FRTL-5) is under the regulation of TSH. In the present report the mechanism by which TSH induces such expression was investigated with the use of human microsomal antibody-positive serum and an indirect i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-2-1140
更新日期:1988-08-01 00:00:00
abstract::The levels of the GnRH receptor (GnRHR) and its messenger RNA depend on the pattern of administration of GnRH. In this study, internal deletion mutants in a luciferase reporter gene vector (GnRHR-pXP2) containing a 1226-bp promoter fragment of mouse GnRHR gene were used to examine the regulation of GnRHR gene transcri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6945
更新日期:1999-08-01 00:00:00
abstract::Our previous work suggests that persistent protein malnutrition in immature domestic fowl (Gallus gallus domesticus) alters ACTH-adrenocortical cell interaction, possibly including ACTH receptors. To investigate this possibility, we measured some ACTH receptor parameters in isolated adrenocortical cells from normal an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-681
更新日期:1988-02-01 00:00:00