Abstract:
:Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on HOB cocultured with endothelial cells. When HOB with high alkaline phosphatase (Al-P) activity and human umbilical vein endothelial cells (HUVEC) with little activity were cultured together, Al-P activity increased, accompanied by an increase in cell number. When HOB and HUVEC were cultured separately, 1,25-(OH)2D3 did not directly stimulate [3H]thymidine incorporation into HUVEC, but stimulated it in the presence of HOB. VEGF did not directly stimulate the Al-P activity of HOB but stimulated it in the presence of HUVEC. The conditioned medium of HOB stimulated the proliferation of HUVEC, and this was partially blocked by anti-VEGF antibody. Conversely, the conditioned medium of HUVEC increased Al-P activity and [3H]thymidine incorporation into HOB, and this was partially blocked by antiinsulin-like growth factor I antibody and BQ-123, a specific antagonist of the endothelin-1 (ET-1) receptor. 1,25-(OH)2D3 stimulated the release of VEGF and ET-1 from HOB and HUVEC, respectively. Furthermore, the 1,25-(OH)2D3-induced release of VEGF was enhanced in HOB cocultured with HUVEC. A quantitative reverse transcription-PCR study revealed that genes for VEGF receptors (Flt-1 and KDR) were expressed in HUVEC, but not in HOB, and that 1,25-(OH)2D3 increased the levels of expression of VEGF receptor genes in endothelial cells only when cocultured with HOB. In summary, we demonstrated that 1,25-(OH)2D3 exerts an anabolic effect on osteoblasts by enhancing their production of VEGF, which stimulates its receptors on endothelial cells, followed by increased production of osteotropic growth factors, such as insulin-like growth factor I and ET-1. These in vitro findings suggest that the VEGF/VEGF receptor system may be involved in both bone formation and bone remodeling in vivo.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Wang DS,Miura M,Demura H,Sato Kdoi
10.1210/endo.138.7.5275subject
Has Abstractpub_date
1997-07-01 00:00:00pages
2953-62issue
7eissn
0013-7227issn
1945-7170journal_volume
138pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Fasting increases hypothalamic neuropeptide Y (NPY) and agouti-related peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.6966
更新日期:1999-10-01 00:00:00
abstract::Studies in rodents demonstrate that the mitogen, IGF-I, stimulates intestinal peptide YY (PYY) expression. To investigate whether the stimulatory influence of IGF-I is exerted at the level of gene transcription, rat PYY 5'-upstream sequences (-2800/+37 bp, -770/+37 bp, -127/+37 bp) fused to the firefly luciferase (luc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0770
更新日期:2004-02-01 00:00:00
abstract::It was shown previously that epidermal growth factor (EGF) plays a role in the testosterone-dependent fetal Wolffian duct differentiation. In this communication, the role of EGF was further investigated by determining whether EGF receptor plays a role in mediating the effect of EGF during Wolffian duct (male duct) dif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404620
更新日期:1993-10-01 00:00:00
abstract::To address controversies of estrogen therapy, in vitro models of perimenopause and prevention vs. treatment modes of 17beta-estradiol (E(2)) exposure were developed and used to assess the neuroprotective efficacy of E(2) against beta-amyloid-1-42 (Abeta(1-42))-induced neurodegeneration in rat primary hippocampal neuro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0495
更新日期:2006-11-01 00:00:00
abstract::In adult male rhesus monkeys, 1 day of fasting leads to a profound suppression of LH secretion that is rapidly reversed with refeeding. To test the hypothesis that changes in insulin secretion during fasting and refeeding mediate the changes in LH secretion, blood samples for LH measurement were obtained between 0900-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641210
更新日期:1996-06-01 00:00:00
abstract::The time sequence of the metabolism of [4-14C] pregnenolone to testosterone in homogenates of human and rat testis was studied with special emphasis on the chain of events in the early 15 min of incubation. The incubations were performed at 32 C in the presence of NAD and a NADPH-generating system. The various interme...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1909
更新日期:1987-05-01 00:00:00
abstract::Thyroid hormones are essential for normal development and metabolism. Their synthesis requires transport of iodide into thyroid follicles. The mechanisms involving the apical efflux of iodide into the follicular lumen are poorly elucidated. The discovery of mutations in the SLC26A4 gene in patients with Pendred syndro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1548
更新日期:2012-01-01 00:00:00
abstract::In previous studies we have shown that aromatase cytochrome P450 (P450arom) mRNA and protein increase markedly in luteal tissue between days 10-19 of gestation, whereas cholesterol side-chain cleavage cytochrome P450 (P450scc) appears to be constitutively maintained regardless of hormonal changes occurring during preg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1673
更新日期:1989-09-01 00:00:00
abstract::To assess a potential underlying rhythm in the sensitivity of the pregnant primate myometrium to oxytocin we infused five pregnant rhesus monkeys (gestational age, 121-142 days) with oxytocin at three different periods of the 24-h day, in the order night, afternoon, and morning, via the maternal inferior vena cava. Ox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1498
更新日期:1989-09-01 00:00:00
abstract::The ontogeny of expression of the CRF gene in the paraventricular hypothalamic nucleus and POMC gene in the pituitary was studied in rats using in situ hybridization histochemistry and Northern blotting techniques, respectively. CRF mRNA was first detected on day 17 of gestation (E17) in the paraventricular nucleus of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-60
更新日期:1989-01-01 00:00:00
abstract::Testicular LH receptor occupancy and steroidogenic responses were measured in adult male rats after intracardiac injections of [125I]iodo-hCG (0.5--5 x 10(6) cpm) mixed with known amounts of nonradioactive hCG to yield doses ranging from 10 ng to 300 micrograms. Uptake of the hormone by the testis was measured in the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-3-982
更新日期:1982-09-01 00:00:00
abstract::Once reserved solely for the treatment of short stature, the now readily available recombinant GH has expanded the use of the hormone to include the treatment of cardiovascular, renal, muscular, skeletal, immunological, psychosocial, and metabolic abnormalities associated with GH deficiency. There are also proposals f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1333
更新日期:2007-06-01 00:00:00
abstract::Functional disruption of either MC3R or MC4R results in obesity, implicating both in the control of energy homeostasis. The ligands for these receptors are derived from the prohormone proopiomelancortin (POMC), which is posttranslationally processed to produce a set of melanocortin peptides with a range of activities ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0866
更新日期:2006-12-01 00:00:00
abstract::Dispersed cells from whole testes or from isolated interstitial tissue of mature rats, yielded two distinct populations of Leydig cells when subjected to centrifugation in a 0-40% metrizamide gradient. One population (I) was found in a fractions with a density of 1.085-1.117 g/cm3, and the other population (II) was fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-5-1424
更新日期:1980-05-01 00:00:00
abstract::Removal of the 18-hydroxy group of the hemiacetal form of aldosterone transforms its activity from that of pure agonist to predominant antagonist. The 18-deoxy derivative possesses one third of the binding affinity of aldosterone for the cytoplasmic mineralocorticoid receptor of rat kidney and exhibits an approximate ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-5-1352
更新日期:1979-05-01 00:00:00
abstract::A 28,000 mol wt vitamin D-dependent calcium-binding protein (CaBP), first isolated from avian intestine, has recently been shown to exist in kidney and other tissues. To study the mechanism regulating the production of renal CaBP, we used primary cultures of chick kidney cells as an in vitro model. Renal cortical tubu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-894
更新日期:1987-03-01 00:00:00
abstract::Diagnostic tests of hypothalamic-pituitary-adrenocortical function in psychiatric illness largely report the interaction of hypothalamic secretagogues with glucocorticoids at the pituitary level. This study investigated whether the efficiency of glucocorticoid inhibition is subject to modulation by intracellular proce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220489
更新日期:2002-10-01 00:00:00
abstract::Estrogens have been implicated in the regulation of prolactin gene expression in man, although previous studies have not defined the molecular mechanism whereby estradiol activates the human prolactin gene promoter (hPrl). We found that estradiol induced a reproducible 1.8-fold activation of the hPrl gene promoter, us...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1066
更新日期:2008-02-01 00:00:00
abstract::Bovine placental lactogen (bPL) was found to be as potent as human GH (hGH) in its ability to bind to soluble full-size recombinant hGH-binding protein (hGHBP) and to membrane-embedded hGH receptor in intact IM-9 human lymphocytes. bPL was also capable of forming a 1:2 complex with hGHBP, although the structure of thi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867580
更新日期:1995-03-01 00:00:00
abstract::The current study was designed to investigate the ability of prostaglandin F2 alpha (PGF2 alpha) to activate a second messenger system (phosphatidylinositol pathway) in corpora lutea (CL) of rhesus monkeys. Activation of this pathway was assessed by monitoring the hydrolysis of phosphatidylinositol to inositol phospha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.2.1639020
更新日期:1992-08-01 00:00:00
abstract::Insulin and epidermal growth factor (EGF) share a number of metabolic actions, including stimulation of protein synthesis and growth in certain tissues, and activation of apparent receptor tyrosine kinase activities. We have shown that insulin and EGF promote the phosphorylation of a number of intracellular proteins i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-141
更新日期:1984-01-01 00:00:00
abstract::The intermediary role of the putative inhibitory regulatory membrane protein "Ni" in the "antigonadal" activity of the neurohypophysial hormones was investigated in vitro with the use of a primary culture of rat testicular cells. To this end, use was made of the pertussis toxin (PT) probe, an exotoxin of Bordetella pe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-839
更新日期:1984-08-01 00:00:00
abstract::Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of the u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00396
更新日期:2017-11-01 00:00:00
abstract::Extensive remodeling of the follicular extracellular matrix occurs during the process of ovulation. This remodeling involves the breakdown of collagen, which is regulated, in part, by the action of the metalloproteinase collagenase and its associated inhibitors. In the present study, follicular metalloproteinase inhib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-1-63
更新日期:1990-07-01 00:00:00
abstract::In young adult female rats, the patterns of inhibin subunit mRNA expression during the estrous cycle are regulated by cyclic changes in gonadotropin secretion and follicular development. Since there are distinct alterations in profiles of both hormone secretion and folliculogenesis during the reproductive lifespan of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419130
更新日期:1993-01-01 00:00:00
abstract::Peptide-YY (PYY) is a novel enteric peptide that is structurally related to pancreatic polypeptide and neuropeptide-Y. The objectives of the present experiments were to characterize the following aspects of PYY metabolism: the distribution of PYY in the canine gastrointestinal tract, the release of PYY in response to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1438
更新日期:1989-03-01 00:00:00
abstract::Glucocorticoid (Gc) excess, from endogenous overproduction in disorders of the hypothalamic-pituitary-adrenal axis or exogenous medical therapy, is recognized to cause adverse metabolic side effects. The Gc receptor (GR) is widely expressed throughout the body, including brain regions such as the hypothalamus. However...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1571
更新日期:2016-11-01 00:00:00
abstract::Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunoh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8588
更新日期:2002-01-01 00:00:00
abstract::Alpha-calcitonin gene-related peptide (alphaCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary physiological role has thus far remained obscure. Increased lipid content, particularly in skeletal muscle and liver, is ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0583
更新日期:2008-01-01 00:00:00
abstract::We have previously demonstrated that CGP 42112 (AT(2) agonist > or =1 nM) markedly reduces catecholamine biosynthesis through AT(2), which is the major angiotensin II (AngII) receptor subtype in cultured porcine chromaffin cells. Also, we have shown that CGP 42112 (> or =1 nM) induces a reduction in cGMP production in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8263
更新日期:2001-07-01 00:00:00